Reviewed, UniProtKB/Swiss-Prot P33438 (GLT_DROME)

Names and origin

Protein names

Recommended name:
    Glutactin

Gene names

Name:	Glt
ORF Names:	CG9280

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1026 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Not known. Binds calcium ions.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane.
Post-translational modification	Extensively O-glycosylated and also N-glycosylated. About four tyrosines are sulfated.
Sequence similarities	In the N-terminal section; belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Signal
Ligand	Calcium
PTM	Disulfide bond Glycoprotein Sulfation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	basement membrane Ref.1 Inferred from direct assay. Source: FlyBase extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17
	Chain	18 – 1026	1009	Glutactin		PRO_0000008639
Regions
	Compositional bias	595 – 615	21	Thr-rich
	Compositional bias	616 – 1026	411	Gln/Glu-rich
	Compositional bias	647 – 749	103	Arg-rich
Amino acid modifications
	Modified residue	26	1	Sulfotyrosine Potential
	Modified residue	29	1	Sulfotyrosine Potential
	Modified residue	182	1	Sulfotyrosine Potential
	Modified residue	559	1	Sulfotyrosine Potential
	Modified residue	645	1	Sulfotyrosine Potential
	Modified residue	727	1	Sulfotyrosine Potential
	Modified residue	836	1	Sulfotyrosine Potential
	Modified residue	862	1	Sulfotyrosine Potential
	Modified residue	865	1	Sulfotyrosine Potential
	Modified residue	868	1	Sulfotyrosine Potential
	Modified residue	922	1	Sulfotyrosine Potential
	Modified residue	928	1	Sulfotyrosine Potential
	Modified residue	981	1	Sulfotyrosine Potential
	Modified residue	984	1	Sulfotyrosine Potential
	Modified residue	1006	1	Sulfotyrosine Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential
	Glycosylation	402	1	N-linked (GlcNAc...) Potential
	Glycosylation	810	1	N-linked (GlcNAc...) Potential
	Disulfide bond	123 ↔ 145		By similarity
	Disulfide bond	298 ↔ 316		By similarity
Experimental info
	Sequence conflict	919 – 921	3	Missing in CAA37380. Ref.1
	Sequence conflict	961	1	S → L in CAA37380. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33438-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: EEB11216C4DC9DF6	FASTA	1,026	118,749
	10 20 30 40 50 60 MKPLLLVLAL CGAQVHAHSV GLRPDYNDYS DEDTRRDWLP EPLKPVPWQS ETRYAQPQEA 70 80 90 100 110 120 VVQAPEVGQI LGISGHKTIA NRPVNAFLGI RYGTVGGGLA RFQAAQPIGY QGRVNATVQS 130 140 150 160 170 180 PNCAQFPELD RLRLSESRGE NVDDCLTLDI YAPEGANQLP VLVFVHGEML FDGGSEEAQP 190 200 210 220 230 240 DYVLEKDVLL VSINYRLAPF GFLSALTDEL PGNVALSDLQ LALEWLQRNV VHFGGNAGQV 250 260 270 280 290 300 TLVGQAGGAT LAHALSLSGR AGNLFQQLIL QSGTALNPYL IDNQPLDTLS TFARLARCPP 310 320 330 340 350 360 PSINPSAQGL KPLYDCLARL PTSQLVAAFE QLLLQNEHLG LTQLGGFKLV VGDPLGFLPS 370 380 390 400 410 420 HPASLATNSS LALPMIIGAT KDASAFIVSR IYDQLARLQS RNVSDYIDVV LRHTAPPSEH 430 440 450 460 470 480 RLWKQWALRE IFTPIQEQTA SLQTVAPGLL ELSNYILYRA PVINSISQSY RSVPAYLYTF 490 500 510 520 530 540 DYRGEHHRFG HLSNPLPFGV DASLSDDSVY LFPYPPEASR LNPLDRSLSR ALVTMWVNFA 550 560 570 580 590 600 TTGVPNPSSG VWPQATSEYG PFLRFTNNQQ SPLELDPHFG EGIYLPNYRV IYKPTTNFSP 610 620 630 640 650 660 PITTTTTTTT TTTTTSRPYA YNPYANWQNR PSQQHPNWHP ADPEYVRAQE ARQQEFIRER 670 680 690 700 710 720 EQRRREQQLR DQQRYPQQEP REQQDERIRQ QREQEERLRQ QREQEERLRQ QRELEERIRQ 730 740 750 760 770 780 QQEREQYERE QQEREQRERE ELERQQRERE QQQPEQQPEY NPEPVNPWGY PVQEPQPDDN 790 800 810 820 830 840 PEDGRLPYPS YEQYGPEGNE NLPETDANRN FSEEDREQQQ QEQLRREQQE QQEREYQLQL 850 860 870 880 890 900 EREQQEREQQ ERGQQEPGPE EYPSYEEYSR ALQEKNAERD RIYAEEQERE RQQQETLLQE 910 920 930 940 950 960 NQQHPEQSLP EEQPTHPNYE AYDGDRSYAE EQEREQQRRD QVEQEREEQP DEDQGEEYER 970 980 990 1000 1010 1020 SPDEEEAAEQ DVLKVEDFPS YEAYLEAATK LREEQEEQEK LEEERYRAQQ EEEDRIQAER ERNSRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Glutactin, a novel Drosophila basement membrane-related glycoprotein with sequence similarity to serine esterases." Olson P.F., Fessler L.I., Nelson R.E., Sterne R.E., Campbell A.G., Fessler J.H. EMBO J. 9:1219-1227(1990) [PubMed: 2108864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, SULFATION. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53286 Genomic DNA. Translation: CAA37380.1. AE014134 Genomic DNA. Translation: AAN10678.1. AE014134 Genomic DNA. Translation: AAN10679.1. AY069776 mRNA. Translation: AAL39921.1.
PIR	S12519.
RefSeq	NP_477504.1. NP_723408.1. NP_723409.1.
UniGene	Dm.14600
3D structure databases
HSSP	HSSP built from PDB template 1F8U based on UniProtKB P22303.
ModBase	Search...
Protein-protein interaction databases
IntAct	P33438. 4 interactions.
Genome annotation databases
Ensembl	FBgn0001114. Drosophila melanogaster. [Contig view]
GeneID	34193.
KEGG	dme:Dmel_CG9280.
Organism-specific databases
FlyBase	FBgn0001114. Glt.
Phylogenomic databases
HOGENOM	P33438.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-008072-MON. DMEL-XXX-02:DMEL-XXX-02-008073-MON. DMEL-XXX-02:DMEL-XXX-02-008074-MON.
Gene expression databases
ArrayExpress	P33438.
GermOnline	CG9280. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019819. Carboxylesterase_B_CS. [Graphical view]
PANTHER	PTHR11559. CarbesteraseB. 1 hit.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	787340.

Entry information

Entry name

GLT_DROME

Accession

Primary (citable) accession number: P33438
Secondary accession number(s): A4V0G5, Q9VLJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	March 1, 2004
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project