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Protein

72 kDa type IV collagenase

Gene

Mmp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).By similarity
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).By similarity
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.1 Publication

Catalytic activityi

Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi102Zinc 2; in inhibited formBy similarity1
Metal bindingi134Calcium 1By similarity1
Metal bindingi168Calcium 2By similarity1
Metal bindingi178Zinc 1By similarity1
Metal bindingi180Zinc 1By similarity1
Metal bindingi185Calcium 3By similarity1
Metal bindingi186Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi193Zinc 1By similarity1
Metal bindingi200Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi202Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi204Calcium 2By similarity1
Metal bindingi206Zinc 1By similarity1
Metal bindingi208Calcium 3By similarity1
Metal bindingi209Calcium 1By similarity1
Metal bindingi211Calcium 3By similarity1
Metal bindingi403Zinc 2; catalyticBy similarity1
Active sitei404PROSITE-ProRule annotation1
Metal bindingi407Zinc 2; catalyticBy similarity1
Metal bindingi413Zinc 2; catalyticBy similarity1
Metal bindingi478Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi523Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi571Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi620Calcium 4; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • blood vessel maturation Source: MGI
  • bone trabecula formation Source: MGI
  • cellular response to amino acid stimulus Source: MGI
  • collagen catabolic process Source: MGI
  • embryo implantation Source: MGI
  • endodermal cell differentiation Source: Ensembl
  • face morphogenesis Source: MGI
  • intramembranous ossification Source: MGI
  • positive regulation of innate immune response Source: MGI
  • positive regulation of vascular smooth muscle cell proliferation Source: MGI
  • proteolysis Source: MGI
  • response to hypoxia Source: MGI
  • skeletal system morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Angiogenesis, Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-3928665. EPH-ephrin mediated repulsion of cells.

Protein family/group databases

MEROPSiM10.003.

Names & Taxonomyi

Protein namesi
Recommended name:
72 kDa type IV collagenase (EC:3.4.24.24)
Alternative name(s):
72 kDa gelatinase
Gelatinase A
Matrix metalloproteinase-2
Short name:
MMP-2
Cleaved into the following chain:
Gene namesi
Name:Mmp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97009. Mmp2.

Subcellular locationi

Isoform 1 :
  • Secretedextracellular spaceextracellular matrix By similarity
  • Membrane By similarity
  • Nucleus By similarity

  • Note: Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular matrix Source: MGI
  • extracellular space Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
  • sarcomere Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3095.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29By similarityAdd BLAST29
PropeptideiPRO_000002871630 – 109Activation peptideAdd BLAST80
ChainiPRO_0000028717110 – 66272 kDa type IV collagenaseAdd BLAST553
ChainiPRO_0000391627445 – 662PEXBy similarityAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi233 ↔ 259PROSITE-ProRule annotation
Disulfide bondi247 ↔ 274PROSITE-ProRule annotation
Disulfide bondi291 ↔ 317PROSITE-ProRule annotation
Disulfide bondi305 ↔ 332PROSITE-ProRule annotation
Disulfide bondi349 ↔ 375PROSITE-ProRule annotation
Disulfide bondi363 ↔ 390PROSITE-ProRule annotation
Disulfide bondi471 ↔ 662PROSITE-ProRule annotation
Glycosylationi575N-linked (GlcNAc...)Sequence analysis1
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro (By similarity).By similarity
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3) (By similarity). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3 (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP33434.
PeptideAtlasiP33434.
PRIDEiP33434.

PTM databases

PhosphoSitePlusiP33434.

Expressioni

Developmental stagei

Present in unfertilized eggs and at the zygote and cleavage stages. Levels increase at the blastocyst stage and with endoderm differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000031740.
CleanExiMM_MMP2.
ExpressionAtlasiP33434. baseline and differential.
GenevisibleiP33434. MM.

Interactioni

Subunit structurei

Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B (By similarity).By similarity

Protein-protein interaction databases

IntActiP33434. 1 interactor.
STRINGi10090.ENSMUSP00000034187.

Chemistry databases

BindingDBiP33434.

Structurei

3D structure databases

ProteinModelPortaliP33434.
SMRiP33434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini228 – 276Fibronectin type-II 1PROSITE-ProRule annotationAdd BLAST49
Domaini286 – 334Fibronectin type-II 2PROSITE-ProRule annotationAdd BLAST49
Domaini344 – 392Fibronectin type-II 3PROSITE-ProRule annotationAdd BLAST49
Repeati474 – 518Hemopexin 1Add BLAST45
Repeati519 – 565Hemopexin 2Add BLAST47
Repeati567 – 615Hemopexin 3Add BLAST49
Repeati616 – 662Hemopexin 4Add BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 221Collagenase-like 1Add BLAST112
Regioni222 – 396Collagen-bindingAdd BLAST175
Regioni397 – 467Collagenase-like 2Add BLAST71
Regioni414 – 662Required for inhibitor TIMP2 bindingBy similarityAdd BLAST249

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi100 – 107Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 3 fibronectin type-II domains.PROSITE-ProRule annotation
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217926.
HOVERGENiHBG052484.
InParanoidiP33434.
KOiK01398.
OMAiKHESCTS.
OrthoDBiEOG091G03DP.
PhylomeDBiP33434.
TreeFamiTF315428.

Family and domain databases

CDDicd00062. FN2. 3 hits.
cd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di1.10.101.10. 1 hit.
2.10.10.10. 3 hits.
2.110.10.10. 1 hit.
3.40.390.10. 2 hits.
InterProiIPR028708. 72kDa_collagenase.
IPR000562. FN_type2_col-bd.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR013806. Kringle-like.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF29. PTHR10201:SF29. 2 hits.
PfamiPF00040. fn2. 3 hits.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00059. FN2. 3 hits.
SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
SSF57440. SSF57440. 3 hits.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00023. FN2_1. 3 hits.
PS51092. FN2_2. 3 hits.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33434-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEARVAWGAL AGPLRVLCVL CCLLGRAIAA PSPIIKFPGD VAPKTDKELA
60 70 80 90 100
VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP
110 120 130 140 150
RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARALKV
160 170 180 190 200
WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG
210 220 230 240 250
VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GREYSSCTDT
260 270 280 290 300
GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNADGQP CKFPFRFQGT
310 320 330 340 350
SYNSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV
360 370 380 390 400
FPFTFLGNKY ESCTSAGRND GKVWCATTTN YDDDRKWGFC PDQGYSLFLV
410 420 430 440 450
AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSHDDIKG IQELYGPSPD
460 470 480 490 500
ADTDTGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT
510 520 530 540 550
PRDKPTGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWVYSASTL
560 570 580 590 600
ERGYPKPLTS LGLPPDVQQV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM
610 620 630 640 650
DPGFPKLIAD SWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV
660
KFGSIKSDWL GC
Length:662
Mass (Da):74,102
Last modified:February 1, 1994 - v1
Checksum:iC630A7DBDB272F02
GO
Isoform 2 (identifier: P33434-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: Induced by oxidative stress.
Show »
Length:586
Mass (Da):65,883
Checksum:iB8EE47CF5723C3D6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0446321 – 76Missing in isoform 2. CuratedAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84324 mRNA. Translation: AAA39338.1.
BC070430 mRNA. Translation: AAH70430.1.
CCDSiCCDS22523.1. [P33434-1]
PIRiA42496.
RefSeqiNP_032636.1. NM_008610.3. [P33434-1]
UniGeneiMm.29564.

Genome annotation databases

EnsembliENSMUST00000034187; ENSMUSP00000034187; ENSMUSG00000031740. [P33434-1]
GeneIDi17390.
KEGGimmu:17390.
UCSCiuc009mue.1. mouse. [P33434-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84324 mRNA. Translation: AAA39338.1.
BC070430 mRNA. Translation: AAH70430.1.
CCDSiCCDS22523.1. [P33434-1]
PIRiA42496.
RefSeqiNP_032636.1. NM_008610.3. [P33434-1]
UniGeneiMm.29564.

3D structure databases

ProteinModelPortaliP33434.
SMRiP33434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP33434. 1 interactor.
STRINGi10090.ENSMUSP00000034187.

Chemistry databases

BindingDBiP33434.
ChEMBLiCHEMBL3095.

Protein family/group databases

MEROPSiM10.003.

PTM databases

PhosphoSitePlusiP33434.

Proteomic databases

PaxDbiP33434.
PeptideAtlasiP33434.
PRIDEiP33434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034187; ENSMUSP00000034187; ENSMUSG00000031740. [P33434-1]
GeneIDi17390.
KEGGimmu:17390.
UCSCiuc009mue.1. mouse. [P33434-1]

Organism-specific databases

CTDi4313.
MGIiMGI:97009. Mmp2.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217926.
HOVERGENiHBG052484.
InParanoidiP33434.
KOiK01398.
OMAiKHESCTS.
OrthoDBiEOG091G03DP.
PhylomeDBiP33434.
TreeFamiTF315428.

Enzyme and pathway databases

ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-3928665. EPH-ephrin mediated repulsion of cells.

Miscellaneous databases

ChiTaRSiMmp2. mouse.
PROiP33434.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031740.
CleanExiMM_MMP2.
ExpressionAtlasiP33434. baseline and differential.
GenevisibleiP33434. MM.

Family and domain databases

CDDicd00062. FN2. 3 hits.
cd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di1.10.101.10. 1 hit.
2.10.10.10. 3 hits.
2.110.10.10. 1 hit.
3.40.390.10. 2 hits.
InterProiIPR028708. 72kDa_collagenase.
IPR000562. FN_type2_col-bd.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR013806. Kringle-like.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF29. PTHR10201:SF29. 2 hits.
PfamiPF00040. fn2. 3 hits.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00059. FN2. 3 hits.
SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
SSF57440. SSF57440. 3 hits.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00023. FN2_1. 3 hits.
PS51092. FN2_2. 3 hits.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP2_MOUSE
AccessioniPrimary (citable) accession number: P33434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.