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Protein

Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial

Gene

SDH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-anchoring mono-heme cytochrome b subunit of succinate dehydrogenase (SDH) that is involved in system II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH3 and SDH4 form the membrane dimer that anchors the catalytic dimer formed by SDH1 and SDH2 to the matrix surface of the mitochondrial inner membrane. Electrons originating from the catalytic dimer enter the membrane dimer for ubiquinone reduction.

Cofactori

hemeNote: The heme is bound between the two transmembrane subunits.

Pathwayi: tricarboxylic acid cycle

This protein is involved in the pathway tricarboxylic acid cycle, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93UbiquinoneBy similarity1
Binding sitei97UbiquinoneBy similarity1
Metal bindingi156Iron (heme axial ligand); shared with second transmembrane subunitBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular respiration Source: SGD
  • mitochondrial electron transport, succinate to ubiquinone Source: GO_Central
  • protein import into mitochondrial inner membrane Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YKL141W-MONOMER.
YEAST:YKL141W-MONOMER.
UniPathwayiUPA00223.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial
Gene namesi
Name:SDH3
Synonyms:CYB3
Ordered Locus Names:YKL141W
ORF Names:YKL4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL141W.
SGDiS000001624. SDH3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini51 – 99Mitochondrial matrixSequence analysisAdd BLAST49
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 139Mitochondrial intermembraneSequence analysisAdd BLAST19
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 175Mitochondrial matrixSequence analysisAdd BLAST15
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 198Mitochondrial intermembraneSequence analysis2

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96H → A or D: Decreases quinone reductase activity. 1 Publication1
Mutagenesisi153F → V: Decreases quinone reductase activity. Little effect on complex assembly. 1 Publication1
Mutagenesisi156H → A: Decreases SDH cytochrome b content. 1 Publication1
Mutagenesisi163H → Q: Decreases quinone reductase activity. Little effect on complex assembly. 1 Publication1
Mutagenesisi166W → R: Decreases quinone reductase activity. Little effect on complex assembly. 1 Publication1
Mutagenesisi167D → V: Reduces SDH FAD content. Probably impairs complex assembly. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50Mitochondrion1 PublicationAdd BLAST50
ChainiPRO_000000363851 – 198Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrialAdd BLAST148

Keywords - PTMi

Quinone

Proteomic databases

MaxQBiP33421.
PRIDEiP33421.

Interactioni

Subunit structurei

Forms part of complex II containing four subunits: a flavoprotein (FP), an iron-sulfur protein (IP) and a cytochrome b composed of two integral membrane proteins.

Protein-protein interaction databases

BioGridi33995. 61 interactors.
DIPiDIP-5310N.
IntActiP33421. 1 interactor.
MINTiMINT-502608.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-C51-198[»]
1PB4model-C51-198[»]
ProteinModelPortaliP33421.
SMRiP33421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b560 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000134690.
HOGENOMiHOG000160253.
InParanoidiP33421.
KOiK00236.
OMAiVHRISHL.
OrthoDBiEOG092C5NHG.

Family and domain databases

InterProiIPR018495. Succ_DH_cyt_bsu_CS.
IPR000701. Succ_DH_Fumarate_Rdtase_TM-su.
[Graphical view]
PfamiPF01127. Sdh_cyt. 1 hit.
[Graphical view]
PROSITEiPS01000. SDH_CYT_1. 1 hit.
PS01001. SDH_CYT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAMMVKLGL NKSALLLKPS AFSRAAALSS SRRLLFNTAR TNFLSTSPLK
60 70 80 90 100
NVASEMNTKA AIAEEQILNK QRAKRPISPH LTIYQPQLTW YLSSLHRISL
110 120 130 140 150
VLMGLGFYLF TILFGVSGLL GLGLTTEKVS NWYHQKFSKI TEWSIKGSFA
160 170 180 190
YLFAIHYGGA IRHLIWDTAK ELTLKGVYRT GYALIGFTAV LGTYLLTL
Length:198
Mass (Da):22,068
Last modified:February 1, 1994 - v1
Checksum:iF210FE99AE97607A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → A in CAA52088 (PubMed:8195189).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25464 Genomic DNA. Translation: CAA80957.1.
X73884 Genomic DNA. Translation: CAA52088.1.
Z28141 Genomic DNA. Translation: CAA81982.1.
AY693024 Genomic DNA. Translation: AAT93043.1.
BK006944 Genomic DNA. Translation: DAA09021.1.
PIRiS37970.
RefSeqiNP_012781.1. NM_001179707.1.

Genome annotation databases

EnsemblFungiiYKL141W; YKL141W; YKL141W.
GeneIDi853716.
KEGGisce:YKL141W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25464 Genomic DNA. Translation: CAA80957.1.
X73884 Genomic DNA. Translation: CAA52088.1.
Z28141 Genomic DNA. Translation: CAA81982.1.
AY693024 Genomic DNA. Translation: AAT93043.1.
BK006944 Genomic DNA. Translation: DAA09021.1.
PIRiS37970.
RefSeqiNP_012781.1. NM_001179707.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-C51-198[»]
1PB4model-C51-198[»]
ProteinModelPortaliP33421.
SMRiP33421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33995. 61 interactors.
DIPiDIP-5310N.
IntActiP33421. 1 interactor.
MINTiMINT-502608.

Proteomic databases

MaxQBiP33421.
PRIDEiP33421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL141W; YKL141W; YKL141W.
GeneIDi853716.
KEGGisce:YKL141W.

Organism-specific databases

EuPathDBiFungiDB:YKL141W.
SGDiS000001624. SDH3.

Phylogenomic databases

GeneTreeiENSGT00860000134690.
HOGENOMiHOG000160253.
InParanoidiP33421.
KOiK00236.
OMAiVHRISHL.
OrthoDBiEOG092C5NHG.

Enzyme and pathway databases

UniPathwayiUPA00223.
BioCyciMetaCyc:YKL141W-MONOMER.
YEAST:YKL141W-MONOMER.

Miscellaneous databases

PROiP33421.

Family and domain databases

InterProiIPR018495. Succ_DH_cyt_bsu_CS.
IPR000701. Succ_DH_Fumarate_Rdtase_TM-su.
[Graphical view]
PfamiPF01127. Sdh_cyt. 1 hit.
[Graphical view]
PROSITEiPS01000. SDH_CYT_1. 1 hit.
PS01001. SDH_CYT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDH3_YEAST
AccessioniPrimary (citable) accession number: P33421
Secondary accession number(s): D6VX55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.