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Protein

Protein NIP100

Gene

NIP100

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Motor-binding component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding (By similarity). The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint.By similarity

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • microtubule binding Source: SGD
  • motor activity Source: UniProtKB-KW

GO - Biological processi

  • establishment of mitotic spindle orientation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-34069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NIP100
Alternative name(s):
Protein NIP80
Gene namesi
Name:NIP100
Synonyms:NIP80
Ordered Locus Names:YPL174C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL174C.
SGDiS000006095. NIP100.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835251 – 868Protein NIP100Add BLAST868

Proteomic databases

MaxQBiP33420.

Interactioni

Subunit structurei

Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of the ARP1 filament attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi36009. 519 interactors.
DIPiDIP-1473N.
IntActiP33420. 16 interactors.
MINTiMINT-389199.
STRINGi4932.YPL174C.

Structurei

3D structure databases

ProteinModelPortaliP33420.
SMRiP33420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 84CAP-GlyPROSITE-ProRule annotationAdd BLAST51

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili101 – 175Sequence analysisAdd BLAST75
Coiled coili207 – 375Sequence analysisAdd BLAST169
Coiled coili645 – 776Sequence analysisAdd BLAST132

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00760000119173.
InParanoidiP33420.
KOiK04648.
OMAiTIAHESA.
OrthoDBiEOG092C5PGK.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiView protein in InterPro
IPR036859. CAP-Gly_dom_sf.
IPR000938. CAP-Gly_domain.
PfamiView protein in Pfam
PF01302. CAP_GLY. 1 hit.
SMARTiView protein in SMART
SM01052. CAP_GLY. 1 hit.
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiView protein in PROSITE
PS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P33420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNAGVQVDT NMQKISLQDT VLVNEMKGRV KFIGETQFAK GIWYGIELDK
60 70 80 90 100
PLGKNDGSAN GIRYFDIDLK KANSNGGYYG LFCKKDTLQF YKPDDDEHSL
110 120 130 140 150
LNGNAAQETI KNLQVKCESL ASKLNKIKIE NHELKTSVEK LSTNETVLLS
160 170 180 190 200
KISRLDKLVK ELKVENGNMK THLDNFNHLL DASDSVMAPD LDKGTLLERS
210 220 230 240 250
HLLQGLLDQT KLSYDKAMKV QEDLLEENTQ LLEENAVLSK KISDLGLQLQ
260 270 280 290 300
QTNNTIGDLA LQIEAQSKSS NIVDKLTNDN ILLTSNIKAL NNELEELQAK
310 320 330 340 350
EKLDENLRIT YEQLEQELRL QLSNLQSALE NEKEIAGTYI EENSRLKATL
360 370 380 390 400
ESIEAKTSHK FQSLELKVNT LQEELYQNKL LKKFYQIYEP FAQPHLAALS
410 420 430 440 450
SQLQYLAEVI ESENFGKLEN IEIHIILKVL SSISYALHIY TIKNTPDHLE
460 470 480 490 500
TTLQCFKVNI APISMWLSEF LQRKFSSKQE TAFSICQFLE DNKFLDKDVT
510 520 530 540 550
LILKILHPIL ETTVPKLLAF LRTNSNFNDN DTLCLIGSLY ERSLSLIARI
560 570 580 590 600
DKLIGKEEIS KQDNRLFLYP SCDITLSSIL TILFSDALFL RQDYKRISSL
610 620 630 640 650
KKLEVFFQGI ESLLENITIF PEQPSQQTSD SESQCNIKEG NFSNSLLSDR
660 670 680 690 700
LNEENIRLKE VLVQKENMLT ELETKIKIII GRDLERKTLE ENIKTLKVEL
710 720 730 740 750
NNKNEENCGK TEILNKLKEE NFNLVNRLKN MELKLYQIKD NNTLNKIYLD
760 770 780 790 800
REKVDRVNLV SEIMELRETI RRQIKEQKRV SIDFSWLDEL PAVENKQPFK
810 820 830 840 850
EHINHSLDTL GIEMFNFVST SRILDLKLDQ PLAEDELWHE RDHSYISYLK
860
RKRKNIRLKS QNVVTYYK
Length:868
Mass (Da):100,290
Last modified:November 1, 1997 - v2
Checksum:iA72EA9E938845081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72227 Genomic DNA. Translation: CAA51030.1.
Z73530 Genomic DNA. Translation: CAA97881.1.
BK006949 Genomic DNA. Translation: DAA11260.1.
PIRiS65186.
RefSeqiNP_015151.1. NM_001183988.1.

Genome annotation databases

EnsemblFungiiYPL174C; YPL174C; YPL174C.
GeneIDi855929.
KEGGisce:YPL174C.

Similar proteinsi

Entry informationi

Entry nameiNIP80_YEAST
AccessioniPrimary (citable) accession number: P33420
Secondary accession number(s): D6W3J4, Q08917
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names