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Protein

Protein NIP100

Gene

NIP100

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Motor-binding component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding (By similarity). The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint.By similarity

GO - Molecular functioni

  • microtubule binding Source: SGD
  • motor activity Source: UniProtKB-KW

GO - Biological processi

  • establishment of mitotic spindle orientation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-34069-MONOMER.
ReactomeiR-SCE-5626467. RHO GTPases activate IQGAPs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NIP100
Alternative name(s):
Protein NIP80
Gene namesi
Name:NIP100
Synonyms:NIP80
Ordered Locus Names:YPL174C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL174C.
SGDiS000006095. NIP100.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • dynactin complex Source: SGD
  • dynein complex Source: UniProtKB-KW
  • microtubule Source: UniProtKB-KW
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868Protein NIP100PRO_0000083525Add
BLAST

Proteomic databases

MaxQBiP33420.
PRIDEiP33420.

Interactioni

Subunit structurei

Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of the ARP1 filament attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP1P386964EBI-12049,EBI-2920
JNM1P362243EBI-12049,EBI-9415

GO - Molecular functioni

  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi36009. 281 interactions.
DIPiDIP-1473N.
IntActiP33420. 15 interactions.
MINTiMINT-389199.

Structurei

3D structure databases

ProteinModelPortaliP33420.
SMRiP33420. Positions 19-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 8451CAP-GlyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili101 – 17575Sequence analysisAdd
BLAST
Coiled coili207 – 375169Sequence analysisAdd
BLAST
Coiled coili645 – 776132Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00760000119173.
InParanoidiP33420.
KOiK04648.
OMAiTIAHESA.
OrthoDBiEOG7B31ZC.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNAGVQVDT NMQKISLQDT VLVNEMKGRV KFIGETQFAK GIWYGIELDK
60 70 80 90 100
PLGKNDGSAN GIRYFDIDLK KANSNGGYYG LFCKKDTLQF YKPDDDEHSL
110 120 130 140 150
LNGNAAQETI KNLQVKCESL ASKLNKIKIE NHELKTSVEK LSTNETVLLS
160 170 180 190 200
KISRLDKLVK ELKVENGNMK THLDNFNHLL DASDSVMAPD LDKGTLLERS
210 220 230 240 250
HLLQGLLDQT KLSYDKAMKV QEDLLEENTQ LLEENAVLSK KISDLGLQLQ
260 270 280 290 300
QTNNTIGDLA LQIEAQSKSS NIVDKLTNDN ILLTSNIKAL NNELEELQAK
310 320 330 340 350
EKLDENLRIT YEQLEQELRL QLSNLQSALE NEKEIAGTYI EENSRLKATL
360 370 380 390 400
ESIEAKTSHK FQSLELKVNT LQEELYQNKL LKKFYQIYEP FAQPHLAALS
410 420 430 440 450
SQLQYLAEVI ESENFGKLEN IEIHIILKVL SSISYALHIY TIKNTPDHLE
460 470 480 490 500
TTLQCFKVNI APISMWLSEF LQRKFSSKQE TAFSICQFLE DNKFLDKDVT
510 520 530 540 550
LILKILHPIL ETTVPKLLAF LRTNSNFNDN DTLCLIGSLY ERSLSLIARI
560 570 580 590 600
DKLIGKEEIS KQDNRLFLYP SCDITLSSIL TILFSDALFL RQDYKRISSL
610 620 630 640 650
KKLEVFFQGI ESLLENITIF PEQPSQQTSD SESQCNIKEG NFSNSLLSDR
660 670 680 690 700
LNEENIRLKE VLVQKENMLT ELETKIKIII GRDLERKTLE ENIKTLKVEL
710 720 730 740 750
NNKNEENCGK TEILNKLKEE NFNLVNRLKN MELKLYQIKD NNTLNKIYLD
760 770 780 790 800
REKVDRVNLV SEIMELRETI RRQIKEQKRV SIDFSWLDEL PAVENKQPFK
810 820 830 840 850
EHINHSLDTL GIEMFNFVST SRILDLKLDQ PLAEDELWHE RDHSYISYLK
860
RKRKNIRLKS QNVVTYYK
Length:868
Mass (Da):100,290
Last modified:November 1, 1997 - v2
Checksum:iA72EA9E938845081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72227 Genomic DNA. Translation: CAA51030.1.
Z73530 Genomic DNA. Translation: CAA97881.1.
BK006949 Genomic DNA. Translation: DAA11260.1.
PIRiS65186.
RefSeqiNP_015151.1. NM_001183988.1.

Genome annotation databases

EnsemblFungiiYPL174C; YPL174C; YPL174C.
GeneIDi855929.
KEGGisce:YPL174C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72227 Genomic DNA. Translation: CAA51030.1.
Z73530 Genomic DNA. Translation: CAA97881.1.
BK006949 Genomic DNA. Translation: DAA11260.1.
PIRiS65186.
RefSeqiNP_015151.1. NM_001183988.1.

3D structure databases

ProteinModelPortaliP33420.
SMRiP33420. Positions 19-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36009. 281 interactions.
DIPiDIP-1473N.
IntActiP33420. 15 interactions.
MINTiMINT-389199.

Proteomic databases

MaxQBiP33420.
PRIDEiP33420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL174C; YPL174C; YPL174C.
GeneIDi855929.
KEGGisce:YPL174C.

Organism-specific databases

EuPathDBiFungiDB:YPL174C.
SGDiS000006095. NIP100.

Phylogenomic databases

GeneTreeiENSGT00760000119173.
InParanoidiP33420.
KOiK04648.
OMAiTIAHESA.
OrthoDBiEOG7B31ZC.

Enzyme and pathway databases

BioCyciYEAST:G3O-34069-MONOMER.
ReactomeiR-SCE-5626467. RHO GTPases activate IQGAPs.

Miscellaneous databases

PROiP33420.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Schlenstedt G., Silver P.A.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Silver P.A.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B."
    Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A.
    Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNIP80_YEAST
AccessioniPrimary (citable) accession number: P33420
Secondary accession number(s): D6W3J4, Q08917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.