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Protein

Exportin-T

Gene

LOS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Preferentially interacts with tRNAs with mature 5'- and 3'-termini and does not distinguish between intron-containing and spliced tRNAs. In the nucleus binds to tRNA and to the Ran-GTPases GSP1 or GSP2 in their active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the tRNA from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.16 Publications

GO - Molecular functioni

  • Ran GTPase binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

  • tRNA export from nucleus Source: SGD
  • tRNA processing Source: UniProtKB-KW
  • tRNA re-export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport, tRNA processing

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31964-MONOMER.

Protein family/group databases

TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Exportin-T
Alternative name(s):
Exportin(tRNA)
Karyopherin-beta
tRNA exportin
Gene namesi
Name:LOS1
Ordered Locus Names:YKL205W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL205W.
SGDiS000001688. LOS1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Shuttles between the nucleus and the cytoplasm and the localization is regulated by SNF1 kinase, nutrient supply and stress.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11001100Exportin-TPRO_0000084460Add
BLAST

Proteomic databases

MaxQBiP33418.
PeptideAtlasiP33418.

Interactioni

Subunit structurei

Interacts with CEX1, GSP1, GSP2, NSP1, NUP2 and UTP8.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CEX1Q124532EBI-10188,EBI-31271

GO - Molecular functioni

  • Ran GTPase binding Source: SGD

Protein-protein interaction databases

BioGridi33918. 82 interactions.
DIPiDIP-760N.
IntActiP33418. 5 interactions.
MINTiMINT-399788.

Structurei

3D structure databases

ProteinModelPortaliP33418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the exportin family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007890.
HOGENOMiHOG000111186.
InParanoidiP33418.
KOiK14288.
OMAiCRSFIES.
OrthoDBiEOG7PZS5Q.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
[Graphical view]
PfamiPF08389. Xpo1. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

P33418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERIQQLVN AVNDPRSDVA TKRQAIELLN GIKSSENALE IFISLVINEN
60 70 80 90 100
SNDLLKFYGL STLIELMTEG VNANPNGLNL VKFEITKWLK FQVLGNKQTK
110 120 130 140 150
LPDFLMNKIS EVLTTLFMLM YSDCNGNQWN SFFDDLMSLF QVDSAISNTS
160 170 180 190 200
PSTDGNILLG LEFFNKLCLM INSEIADQSF IRSKESQLKN NNIKDWMRDN
210 220 230 240 250
DIMKLSNVWF QCLKLDEQIV SQCPGLINST LDCIGSFISW IDINLIIDAN
260 270 280 290 300
NYYLQLIYKF LNLKETKISC YNCILAIISK KMKPMDKLAF LNMINLTNEL
310 320 330 340 350
TYYHQAISMN PQIITFDNLE VWESLTKLIT SFGIEFTIII EQVNDDQKLD
360 370 380 390 400
TLYKQSVISN VDSILLEKII PILLEFMNNE FDSITAKTFP FWSNYLAFLK
410 420 430 440 450
KYKASSPNFV PLHKDFLDNF QQICFKRMKF SDDEVTQDDF EEFNETVRFK
460 470 480 490 500
LKNFQEIIVV IDPSLFLNNI SQEISANLMN CKNESWQIFE LTIYQIFNLS
510 520 530 540 550
ECTKNNYFGL NKNEIMTSQP SLTLVRFLNE LLMMKDFLLA IDNEQIQILF
560 570 580 590 600
MELIVKNYNF IFSTSANTAN ATDDDEKYLL ILNIFMSSFA MFNKRENVRL
610 620 630 640 650
RSWYLFTRFL KLTRINLKKI LFANKNLVNE ITNKISPLLH IKVTSINAQG
660 670 680 690 700
TDDNDTIFDN QLYIFEGIGF IITLNNSSQE LTAATANTPI DYDILDQILT
710 720 730 740 750
PLFTQLEGCI TQGASPVVIL ECHHILMAIG TLARGLHIGL VPENQVNNMV
760 770 780 790 800
VNKKLINDSL IHKFSNIAEV ILVTFSFFNK FENIRDASRF TFARLIPILS
810 820 830 840 850
NKILPFINKL IELILSSTDL KSWEMIDFLG FLSQLIHMFH TDTDCYQLFN
860 870 880 890 900
QLLTPLINKV HSIIEEIDEQ HDQQSSSNKP IDTAVTATSV NKNIVVTDSY
910 920 930 940 950
RDKILLKKAY CTFLQSFTNN SVTSILLSDI NRAILPVILN DLVTYTPQEI
960 970 980 990 1000
QETSMMKVSL NVLCNFIKCF GNGTCLDNDD INKDPNLKID GLNEYFIMKC
1010 1020 1030 1040 1050
VPIIFEIPFN PIYKFNIKEG NFKTMAYDLA RLLRELFIVS SNPTTNENEC
1060 1070 1080 1090 1100
VKYLTQIYLP QIQLPQELTI QLVNMLTTMG QKQFEKWFVD NFISVLKQGQ
Length:1,100
Mass (Da):126,820
Last modified:February 1, 1994 - v1
Checksum:i01A91E5FAC78FEDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13941 Unassigned DNA. Translation: AAC37342.1.
Z28205 Genomic DNA. Translation: CAA82050.1.
BK006944 Genomic DNA. Translation: DAA08964.1.
PIRiS38043.
RefSeqiNP_012717.1. NM_001179770.1.

Genome annotation databases

EnsemblFungiiYKL205W; YKL205W; YKL205W.
GeneIDi853630.
KEGGisce:YKL205W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13941 Unassigned DNA. Translation: AAC37342.1.
Z28205 Genomic DNA. Translation: CAA82050.1.
BK006944 Genomic DNA. Translation: DAA08964.1.
PIRiS38043.
RefSeqiNP_012717.1. NM_001179770.1.

3D structure databases

ProteinModelPortaliP33418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33918. 82 interactions.
DIPiDIP-760N.
IntActiP33418. 5 interactions.
MINTiMINT-399788.

Protein family/group databases

TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Proteomic databases

MaxQBiP33418.
PeptideAtlasiP33418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL205W; YKL205W; YKL205W.
GeneIDi853630.
KEGGisce:YKL205W.

Organism-specific databases

EuPathDBiFungiDB:YKL205W.
SGDiS000001688. LOS1.

Phylogenomic databases

GeneTreeiENSGT00390000007890.
HOGENOMiHOG000111186.
InParanoidiP33418.
KOiK14288.
OMAiCRSFIES.
OrthoDBiEOG7PZS5Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-31964-MONOMER.

Miscellaneous databases

NextBioi974505.
PROiP33418.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
[Graphical view]
PfamiPF08389. Xpo1. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix."
    Shen W.-C., Selvakumar D., Stanford D.R., Hopper A.K.
    J. Biol. Chem. 268:19436-19444(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs."
    Hopper A.K., Schultz L.D., Shapiro R.A.
    Cell 19:741-751(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing."
    Hurt D.J., Wang S.S., Lin Y.-H., Hopper A.K.
    Mol. Cell. Biol. 7:1208-1216(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Nuclear pore proteins are involved in the biogenesis of functional tRNA."
    Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C.
    EMBO J. 15:2270-2284(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family."
    Shen W.-C., Stanford D.R., Hopper A.K.
    Genetics 143:699-712(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "tRNA nuclear export in saccharomyces cerevisiae: in situ hybridization analysis."
    Sarkar S., Hopper A.K.
    Mol. Biol. Cell 9:3041-3055(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA."
    Hellmuth K., Lau D.M., Bischoff F.R., Kuenzler M., Hurt E.C., Simos G.
    Mol. Cell. Biol. 18:6374-6386(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GSP1; GSP2; NSP1 AND NUP2.
  10. "A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants."
    Hurt E.C., Hannus S., Schmelzl B., Lau D.M., Tollervey D., Simos G.
    J. Cell Biol. 144:389-401(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Nucleotides of the tRNA D-stem that play an important role in nuclear-tRNA export in Saccharomyces cerevisiae."
    Cleary J.D., Mangroo D.
    Biochem. J. 347:115-122(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "An aminoacylation-dependent nuclear tRNA export pathway in yeast."
    Grosshans H., Hurt E.C., Simos G.
    Genes Dev. 14:830-840(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2."
    Qiu H., Hu C., Anderson J., Bjoerk G.R., Sarkar S., Hopper A.K., Hinnebusch A.G.
    Mol. Cell. Biol. 20:2505-2516(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  15. "The nuclear tRNA aminoacylation-dependent pathway may be the principal route used to export tRNA from the nucleus in Saccharomyces cerevisiae."
    Steiner-Mosonyi M., Mangroo D.
    Biochem. J. 378:809-816(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Retrograde movement of tRNAs from the cytoplasm to the nucleus in Saccharomyces cerevisiae."
    Shaheen H.H., Hopper A.K.
    Proc. Natl. Acad. Sci. U.S.A. 102:11290-11295(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "tRNA actively shuttles between the nucleus and cytosol in yeast."
    Takano A., Endo T., Yoshihisa T.
    Science 309:140-142(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "The localization of nuclear exporters of the importin-beta family is regulated by Snf1 kinase, nutrient supply and stress."
    Quan X., Yu J., Bussey H., Stochaj U.
    Biochim. Biophys. Acta 1773:1052-1061(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  19. "Impaired tRNA nuclear export links DNA damage and cell-cycle checkpoint."
    Ghavidel A., Kislinger T., Pogoutse O., Sopko R., Jurisica I., Emili A.
    Cell 131:915-926(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  20. "Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery."
    McGuire A.T., Mangroo D.
    EMBO J. 26:288-300(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CEX1.
  21. "Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae."
    Strub B.R., Eswara M.B.K., Pierce J.B., Mangroo D.
    Mol. Biol. Cell 18:3845-3859(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GSP1 AND UTP8.

Entry informationi

Entry nameiXPOT_YEAST
AccessioniPrimary (citable) accession number: P33418
Secondary accession number(s): D6VWZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3490 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.