P33417 (IXR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intrastrand cross-link recognition protein

Alternative name(s):
Structure-specific recognition protein
Short name=SSRP

Gene names

Name:	IXR1
Synonyms:	ORD1
Ordered Locus Names:	YKL032C
ORF Names:	YKL245

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to platinated DNA and confers sensitivity to the anticancer drug cisplatin. Activate the expression of the COX5B gene.
Subcellular location	Nucleus Ref.6.
Sequence similarities	Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from genetic interaction PubMed 8538651. Source: SGD cellular response to hypoxia Inferred from mutant phenotype PubMed 19807692. Source: SGD negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11115121. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	damaged DNA binding Inferred from direct assay PubMed 8634251. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 11115121PubMed 19807692. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Intrastrand cross-link recognition protein		PRO_0000048573
Regions
	DNA binding	361 – 429	69	HMG box 1
	DNA binding	434 – 502	69	HMG box 2
	Compositional bias	52 – 118	67	Gln-rich
	Compositional bias	281 – 336	56	Gln-rich
	Compositional bias	565 – 580	16	Gln-rich
Amino acid modifications
	Modified residue	532	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	73	1	Missing in AAA02859. Ref.1
	Sequence conflict	93	1	Y → F in AAA02859. Ref.1
	Sequence conflict	114 – 118	5	Missing Ref.1
	Sequence conflict	207	1	A → T in AAA02859. Ref.1
	Sequence conflict	220 – 221	2	AA → TT in AAA02859. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33417 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: AF3B705AA2C5AC74	FASTA	597	67,856
	10 20 30 40 50 60 MNTGISPKQD DASNSNLLNI GQDHSLQYQG LEHNDSQYRD ASHQTPHQYL NQFQAQPQQQ 70 80 90 100 110 120 QQQQQQQQQQ QQQAPYQGHF QQSPQQQQQN VYYPLPPQSL TQPTSQSQQQ QQQQQQQQYA 130 140 150 160 170 180 NSNSNSNNNV NVNALPQDFG YMQQTGSGQN YPTINQQQFS EFYNSFLSHL TQKQTNPSVT 190 200 210 220 230 240 GTGASSNNNS NNNNVSSGNN STSSNPAQLA ASQLNPATAA AAAANNAAGP ASYLSQLPQV 250 260 270 280 290 300 QRYYPNNMNA LSSLLDPSSA GNAAGNANTA THPGLLPPNL QPQLTHHQQQ MQQQLQLQQQ 310 320 330 340 350 360 QQLQQQQQLQ QQHQLQQQQQ LQQQHHHLQQ QQQQQQHPVV KKLSSTQSRI ERRKQLKKQG 370 380 390 400 410 420 PKRPSSAYFL FSMSIRNELL QQFPEAKVPE LSKLASARWK ELTDDQKKPF YEEFRTNWEK 430 440 450 460 470 480 YRVVRDAYEK TLPPKRPSGP FIQFTQEIRP TVVKENPDKG LIEITKIIGE RWRELDPAKK 490 500 510 520 530 540 AEYTETYKKR LKEWESCYPD ENDPNGNPTG HSHKAMNMNL NMDTKIMENQ DSIEHITANA 550 560 570 580 590 IDSVTGSNSN STNPNTPVSP PISLQQQPLQ QQQQQQQQQQ HMLLADPTTN GSIIKNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ixr1, a yeast protein that binds to platinated DNA and confers sensitivity to cisplatin." Brown S.J., Kellett P.J., Lippard S.J. Science 261:603-605(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The ORD1 gene encodes a transcription factor involved in oxygen regulation and is identical to IXR1, a gene that confers cisplatin sensitivity to Saccharomyces cerevisiae." Lambert J.R., Bilanchone V.W., Cumsky M.G. Proc. Natl. Acad. Sci. U.S.A. 91:7345-7349(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Analysis of an 11.7 kb DNA fragment of chromosome XI reveals a new tRNA gene and four new open reading frames including a leucine zipper protein and a homologue to the yeast mitochondrial regulator ABF2." Purnelle B., Skala J., van Dyck L., Goffeau A. Yeast 10:125-130(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71622 Genomic DNA. Translation: CAB37853.1. L16900 Unassigned DNA. Translation: AAA02859.1. Z28032 Genomic DNA. Translation: CAA81867.1. BK006944 Genomic DNA. Translation: DAA09124.1.
PIR	S37849.
RefSeq	NP_012893.3. NM_001179598.3.
3D structure databases
ProteinModelPortal	P33417.
SMR	P33417. Positions 362-503.
ModBase	Search...
Protein-protein interaction databases
IntAct	P33417. 52 interactions.
STRING	4932.YKL032C.
Proteomic databases
PaxDb	P33417.
PeptideAtlas	P33417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL032C; YKL032C; YKL032C.
GeneID	853836.
KEGG	sce:YKL028W. sce:YKL032C.
Organism-specific databases
CYGD	YKL032c.
SGD	S000001515. IXR1.
Phylogenomic databases
eggNOG	COG5648.
GeneTree	ENSGT00680000100562.
KO	K03136.
OrthoDB	EOG483HF0.
Gene expression databases
Genevestigator	P33417.
GermOnline	YKL032C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.30.10. 2 hits.
InterPro	IPR009071. HMG_box_dom. [Graphical view]
Pfam	PF00505. HMG_box. 2 hits. [Graphical view]
SMART	SM00398. HMG. 2 hits. [Graphical view]
SUPFAM	SSF47095. HMG-box. 2 hits.
PROSITE	PS50118. HMG_BOX_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	975043.

Entry information

Entry name

IXR1_YEAST

Accession

Primary (citable) accession number: P33417
Secondary accession number(s): D6VXQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program