Urea active transporter - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P33413 (DUR3_YEAST)

Last modified November 3, 2009. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Urea active transporter

Gene names

Name:	DUR3
Ordered Locus Names:	YHL016C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for active transport of urea.
Subunit structure	May polymerize.
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	By allophanate or its non-metabolized analog oxalurate. Sensitive to nitrogen catabolite repression.
Sequence similarities	Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

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Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine transport Inferred from direct assay. Source: SGD spermidine transport Inferred from direct assay. Source: SGD urea catabolic process Ref.1 Inferred from expression pattern. Source: SGD urea transport Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct putrescine transmembrane transporter activity Inferred from direct assay. Source: SGD spermidine transmembrane transporter activity Inferred from direct assay. Source: SGD urea transmembrane transporter activity Ref.1 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
FMP45	Q07651	1	EBI-2060312,EBI-2051056
FTR1	P40088	1	EBI-2060312,EBI-7138
GAP1	P19145	1	EBI-2060312,EBI-7314
GPA2	P10823	1	EBI-2060312,EBI-7382
HNM1	P19807	1	EBI-2060312,EBI-8409
KEX1	P09620	1	EBI-2060312,EBI-9653
MEP3	P53390	1	EBI-2060312,EBI-10729
MUP1	P50276	1	EBI-2060312,EBI-11624
OSH7	P38755	1	EBI-2060312,EBI-12641
SDS23	P53172	1	EBI-2060312,EBI-23782
SFK1	P35735	1	EBI-2060312,EBI-26678
SLM1	P40485	1	EBI-2060312,EBI-25172
SNQ2	P32568	1	EBI-2060312,EBI-17590
TCB3	Q03640	1	EBI-2060312,EBI-27872
TPO1	Q07824	1	EBI-2060312,EBI-38106

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 735

735

Urea active transporter

PRO_0000105397

Regions

Topological domain

1 – 14

Cytoplasmic Potential

Transmembrane

15 – 35

Potential

Topological domain

36 – 85

Extracellular Potential

Transmembrane

86 – 106

Potential

Topological domain

107 – 130

Cytoplasmic Potential

Transmembrane

131 – 151

Potential

Topological domain

152 – 166

Extracellular Potential

Transmembrane

167 – 187

Potential

Topological domain

188 – 189

Cytoplasmic Potential

Transmembrane

190 – 210

Potential

Topological domain

211 – 253

Extracellular Potential

Transmembrane

254 – 274

Potential

Topological domain

275 – 295

Cytoplasmic Potential

Transmembrane

296 – 316

Potential

Topological domain

317 – 343

Extracellular Potential

Transmembrane

344 – 364

Potential

Topological domain

365 – 403

Cytoplasmic Potential

Transmembrane

404 – 424

Potential

Topological domain

425

Extracellular Potential

Transmembrane

426 – 446

Potential

Topological domain

447 – 454

Cytoplasmic Potential

Transmembrane

455 – 475

Potential

Topological domain

476 – 496

Extracellular Potential

Transmembrane

497 – 517

Potential

Topological domain

518 – 618

101

Cytoplasmic Potential

Transmembrane

619 – 639

Potential

Topological domain

640 – 650

Extracellular Potential

Transmembrane

651 – 671

Potential

Topological domain

672 – 735

Cytoplasmic Potential

Experimental info

Sequence conflict

58 – 63

GLVAAA → RLSGCS in AAA34582. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P33413-1 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: FD1252DB838BF5CF
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FASTA

735

80,617

        10         20         30         40         50         60 
MGEFKPPLPQ GAGYAIVLGL GAVFAGMMVL TTYLLKRYQK EIITAEEFTT AGRSVKTGLV 

        70         80         90        100        110        120 
AAAVVSSWIW CSTLLTSSTK EYADGIFGGY AYAAGACFQI IAFAILAIKT KQMAPNAHTY 

       130        140        150        160        170        180 
LELVRTRYGK IGHGCYLFYA IATNILVTSM LLTSGSAVFS DLTGMNTIAS CFLLPVGVVV 

       190        200        210        220        230        240 
YTLFGGIKAT FLTDYMHTCV IIIIVLVFAF KVYATSDVLG SPGKVYDLVR EAAKRHPVDG 

       250        260        270        280        290        300 
NYQGEYMTMT SKSAGILLII NLIGNFGTVF LDNGYWNKAI SASPAASLKA YAIGGLAWFA 

       310        320        330        340        350        360 
VPSLISLTMG LACLAVETSP NFPTYPDPLT SFQANSGLVL PAAAIAIMGK GGAVASLLMI 

       370        380        390        400        410        420 
FMAVTSAMSA ELIAVSSVFT YDIYREYIDP RASGKKLIYT SHVACIFFGL AMSGFSVGLY 

       430        440        450        460        470        480 
YGGISMGYIY EMMGIIISSA VLPVVLTLCS KDMNLVAAVV SPILGTGLAI MSWLVCTKSL 

       490        500        510        520        530        540 
YKELTVDTTF MDYPMLTGNL VALLSPAIFI PILTYVFKPQ NFDWEKMKDI TRVDETAELV 

       550        560        570        580        590        600 
QADPDIQLYD AEANDKEQEE ETNSLVSDSE KNDVRVNNEK LIEPNLGVVI SNAIFQEDDT 

       610        620        630        640        650        660 
QLQNELDEEQ RELARGLKIA YFLCVFFALA FLVVWPMPMY GSKYIFSKKF FTGWVVVMII 

       670        680        690        700        710        720 
WLFFSAFAVC IYPLWEGRHG IYTTLRGLYW DLSGQTYKLR EWQNSNPQDL HVVTSQISAR 

       730 
AHRQSSHFGQ VDEII

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae." Elberry H.M., Majumdar M.L., Cunningham T.S., Sumrada R.A., Cooper T.G. J. Bacteriol. 175:4688-4698(1993) [PubMed: 8335627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TCY1.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L19875 Genomic DNA. Translation: AAA34582.1. U11582 Genomic DNA. Translation: AAB65069.1. AY693170 Genomic DNA. Translation: AAT93189.1.
PIR	S46830.
RefSeq	NP_011847.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P33413. 20 interactions.
STRING	P33413.
Protein family/group databases
TCDB	2.A.21.6.1. solute:sodium symporter (SSS) family.
Genome annotation databases
Ensembl	YHL016C; YHL016C; YHL016C; Saccharomyces cerevisiae. [Genome view]
GeneID	856370.
GenomeReviews	Gene locus YHL016C in contig U00093_GR.
KEGG	sce:YHL016C.
NMPDR	fig\|4932.3.peg.2986.
Organism-specific databases
CYGD	YHL016c.
SGD	S000001008. DUR3.
Phylogenomic databases
HOGENOM	P33413.
OMA	GQTYKLR.
Gene expression databases
ArrayExpress	P33413.
Genevestigator	P33413.
GermOnline	YHL016C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001734. Na/solute_symporter. IPR018212. Na/solute_symporter_CS. IPR019900. Na/solute_symporter_subgr. [Graphical view]
PANTHER	PTHR11819. Na/solut_symport. 1 hit.
Pfam	PF00474. SSF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00813. sss. 1 hit.
PROSITE	PS00456. NA_SOLUT_SYMP_1. False negative. PS00457. NA_SOLUT_SYMP_2. False negative. PS50283. NA_SOLUT_SYMP_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981846.

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Entry information

Entry name

DUR3_YEAST

Accession

Primary (citable) accession number: P33413

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents