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P33413 (DUR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urea active transporter
Gene names
Name:DUR3
Ordered Locus Names:YHL016C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for active transport of urea.

Subunit structure

May polymerize.

Subcellular location

Membrane; Multi-pass membrane protein.

Induction

By allophanate or its non-metabolized analog oxalurate. Sensitive to nitrogen catabolite repression.

Sequence similarities

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Urea active transporter
PRO_0000105397

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Helical; Potential
Topological domain36 – 8550Extracellular Potential
Transmembrane86 – 10621Helical; Potential
Topological domain107 – 13024Cytoplasmic Potential
Transmembrane131 – 15121Helical; Potential
Topological domain152 – 16615Extracellular Potential
Transmembrane167 – 18721Helical; Potential
Topological domain188 – 1892Cytoplasmic Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 25343Extracellular Potential
Transmembrane254 – 27421Helical; Potential
Topological domain275 – 29521Cytoplasmic Potential
Transmembrane296 – 31621Helical; Potential
Topological domain317 – 34327Extracellular Potential
Transmembrane344 – 36421Helical; Potential
Topological domain365 – 40339Cytoplasmic Potential
Transmembrane404 – 42421Helical; Potential
Topological domain4251Extracellular Potential
Transmembrane426 – 44621Helical; Potential
Topological domain447 – 4548Cytoplasmic Potential
Transmembrane455 – 47521Helical; Potential
Topological domain476 – 49621Extracellular Potential
Transmembrane497 – 51721Helical; Potential
Topological domain518 – 618101Cytoplasmic Potential
Transmembrane619 – 63921Helical; Potential
Topological domain640 – 65011Extracellular Potential
Transmembrane651 – 67121Helical; Potential
Topological domain672 – 73564Cytoplasmic Potential

Experimental info

Sequence conflict58 – 636GLVAAA → RLSGCS in AAA34582. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P33413 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: FD1252DB838BF5CF

FASTA73580,617
        10         20         30         40         50         60 
MGEFKPPLPQ GAGYAIVLGL GAVFAGMMVL TTYLLKRYQK EIITAEEFTT AGRSVKTGLV 

        70         80         90        100        110        120 
AAAVVSSWIW CSTLLTSSTK EYADGIFGGY AYAAGACFQI IAFAILAIKT KQMAPNAHTY 

       130        140        150        160        170        180 
LELVRTRYGK IGHGCYLFYA IATNILVTSM LLTSGSAVFS DLTGMNTIAS CFLLPVGVVV 

       190        200        210        220        230        240 
YTLFGGIKAT FLTDYMHTCV IIIIVLVFAF KVYATSDVLG SPGKVYDLVR EAAKRHPVDG 

       250        260        270        280        290        300 
NYQGEYMTMT SKSAGILLII NLIGNFGTVF LDNGYWNKAI SASPAASLKA YAIGGLAWFA 

       310        320        330        340        350        360 
VPSLISLTMG LACLAVETSP NFPTYPDPLT SFQANSGLVL PAAAIAIMGK GGAVASLLMI 

       370        380        390        400        410        420 
FMAVTSAMSA ELIAVSSVFT YDIYREYIDP RASGKKLIYT SHVACIFFGL AMSGFSVGLY 

       430        440        450        460        470        480 
YGGISMGYIY EMMGIIISSA VLPVVLTLCS KDMNLVAAVV SPILGTGLAI MSWLVCTKSL 

       490        500        510        520        530        540 
YKELTVDTTF MDYPMLTGNL VALLSPAIFI PILTYVFKPQ NFDWEKMKDI TRVDETAELV 

       550        560        570        580        590        600 
QADPDIQLYD AEANDKEQEE ETNSLVSDSE KNDVRVNNEK LIEPNLGVVI SNAIFQEDDT 

       610        620        630        640        650        660 
QLQNELDEEQ RELARGLKIA YFLCVFFALA FLVVWPMPMY GSKYIFSKKF FTGWVVVMII 

       670        680        690        700        710        720 
WLFFSAFAVC IYPLWEGRHG IYTTLRGLYW DLSGQTYKLR EWQNSNPQDL HVVTSQISAR 

       730 
AHRQSSHFGQ VDEII

« Hide

References

« Hide 'large scale' references
[1]"Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae."
Elberry H.M., Majumdar M.L., Cunningham T.S., Sumrada R.A., Cooper T.G.
J. Bacteriol. 175:4688-4698(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: TCY1.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L19875 Genomic DNA. Translation: AAA34582.1.
U11582 Genomic DNA. Translation: AAB65069.1.
AY693170 Genomic DNA. Translation: AAT93189.1.
BK006934 Genomic DNA. Translation: DAA06669.1.
PIRS46830.
RefSeqNP_011847.1. NM_001179096.1.

3D structure databases

ProteinModelPortalP33413.
ModBaseSearch...

Protein-protein interaction databases

IntActP33413. 15 interactions.
STRING4932.YHL016C.

Protein family/group databases

TCDB2.A.21.6.1. solute:sodium symporter (SSS) family.

Proteomic databases

PaxDbP33413.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL016C; YHL016C; YHL016C.
GeneID856370.
KEGGsce:YHL016C.

Organism-specific databases

CYGDYHL016c.
SGDS000001008. DUR3.

Phylogenomic databases

eggNOGCOG0591.
HOGENOMHOG000106394.
OMACLAVETS.
OrthoDBEOG46T68V.

Gene expression databases

GenevestigatorP33413.
GermOnlineYHL016C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001734. Na/solute_symporter.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERPTHR11819. PTHR11819. 1 hit.
PfamPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00813. sss. 1 hit.
PROSITEPS00456. NA_SOLUT_SYMP_1. False negative.
PS00457. NA_SOLUT_SYMP_2. False negative.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981846.

Entry information

Entry nameDUR3_YEAST
AccessionPrimary (citable) accession number: P33413
Secondary accession number(s): D3DKP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents