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Protein

pH-response transcription factor pacC/RIM101

Gene

RIM101

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri146 – 171C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri182 – 206C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri212 – 234C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • ascospore formation Source: SGD
  • barrier septum assembly Source: SGD
  • cellular response to alkaline pH Source: SGD
  • cellular response to anoxia Source: SGD
  • fungal-type cell wall biogenesis Source: SGD
  • meiotic nuclear division Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31047-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
pH-response transcription factor pacC/RIM101
Alternative name(s):
Regulator of IME2 protein 1
pH-response regulator protein RIM101
Gene namesi
Name:RIM101
Synonyms:RIM1
Ordered Locus Names:YHL027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL027W.
SGDiS000001019. RIM101.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153C → S: Abolishes transcriptional activation of target genes. 1 Publication1
Mutagenesisi189C → S: Abolishes transcriptional activation of target genes. 1 Publication1
Mutagenesisi217C → S: Abolishes transcriptional activation of target genes. 1 Publication1
Mutagenesisi255S → A: Reduces transcriptional activation of target genes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468471 – 625pH-response transcription factor pacC/RIM101Add BLAST625

Post-translational modificationi

Activated by C-terminal proteolytic cleavage. At neutral to alkaline ambient pH, the signaling protease (probably RIM13) cleaves RIM101, removing a C-terminal 8 kDa peptide to yield the active form.1 Publication

Proteomic databases

MaxQBiP33400.
PRIDEiP33400.

PTM databases

iPTMnetiP33400.

Interactioni

Subunit structurei

Binds to DNA. Interacts with RIM20, which probably binds to the two YPX[LI] motifs and is required for proteolytic processing.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB3P436032EBI-14422,EBI-22980
RIM20Q120333EBI-14422,EBI-38947
YSC84P327932EBI-14422,EBI-24460

Protein-protein interaction databases

BioGridi36395. 369 interactors.
DIPiDIP-1564N.
IntActiP33400. 10 interactors.
MINTiMINT-386832.

Structurei

3D structure databases

ProteinModelPortaliP33400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 234Nuclear localization signalBy similarity7
Motifi450 – 453YPX[LI] motif 14
Motifi620 – 623YPX[LI] motif 24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 120Ser-richAdd BLAST45
Compositional biasi131 – 140Poly-Asp10
Compositional biasi579 – 601Asp/Glu-rich (acidic)Add BLAST23

Sequence similaritiesi

Belongs to the pacC/RIM101 family.Curated
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri146 – 171C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri182 – 206C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri212 – 234C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

InParanoidiP33400.
OrthoDBiEOG092C1EDI.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPLEDLLNK ENGTAAPQHS RESIVENGTD VSNVTKKDGL PSPNLSKRSS
60 70 80 90 100
DCSKRPRIRC TTEAIGLNGQ EDERMSPGST SSSCLPYHST SHLNTPPYDL
110 120 130 140 150
LGASAVSPTT SSSSDSSSSS PLAQAHNPAG DDDDADNDGD SEDITLYCKW
160 170 180 190 200
DNCGMIFNQP ELLYNHLCHD HVGRKSHKNL QLNCHWGDCT TKTEKRDHIT
210 220 230 240 250
SHLRVHVPLK PFGCSTCSKK FKRPQDLKKH LKIHLESGGI LKRKRGPKWG
260 270 280 290 300
SKRTSKKNKS CASDAVSSCS ASVPSAIAGS FKSHSTSPQI LPPLPVGISQ
310 320 330 340 350
HLPSQQQQRA ISLNQLCSDE LSQYKPVYSP QLSARLQTIL PPLYYNNGST
360 370 380 390 400
VSQGANSRSM NVYEDGCSNK TIANATQFFT KLSRNMTNNY ILQQSGGSTE
410 420 430 440 450
SSSSSGRIPV AQTSYVQPPN APSYQSVQGG SSISATANTA TYVPVRLAKY
460 470 480 490 500
PTGPSLTEHL PPLHSNTAGG VFNRQSQYAM PHYPSVRAAP SYSSSGCSIL
510 520 530 540 550
PPLQSKIPML PSRRTMAGGT SLKPNWEFSL NQKSCTNDII MSKLAIEEVD
560 570 580 590 600
DESEIEDDFV EMLGIVNIIK DYLLCCVMED LDDEESEDKD EENAFLQESL
610 620
EKLSLQNQMG TNSVRILTKY PKILV
Length:625
Mass (Da):68,232
Last modified:February 1, 1995 - v2
Checksum:iAD71E7B2D679E17E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68N → K in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti90T → S in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti111S → P in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti249W → L in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti261C → S in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti276A → G in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti308Q → QQQQ (PubMed:8367297).Curated1
Sequence conflicti358R → Q in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti486V → I in CAA51462 (PubMed:8367297).Curated1
Sequence conflicti519G → E in CAA51462 (PubMed:8367297).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72960 Genomic DNA. Translation: CAA51462.1.
U11583 Genomic DNA. Translation: AAB65039.1.
AY693138 Genomic DNA. Translation: AAT93157.1.
BK006934 Genomic DNA. Translation: DAA06658.1.
PIRiS48941.
RefSeqiNP_011836.1. NM_001179107.1.

Genome annotation databases

EnsemblFungiiYHL027W; YHL027W; YHL027W.
GeneIDi856358.
KEGGisce:YHL027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72960 Genomic DNA. Translation: CAA51462.1.
U11583 Genomic DNA. Translation: AAB65039.1.
AY693138 Genomic DNA. Translation: AAT93157.1.
BK006934 Genomic DNA. Translation: DAA06658.1.
PIRiS48941.
RefSeqiNP_011836.1. NM_001179107.1.

3D structure databases

ProteinModelPortaliP33400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36395. 369 interactors.
DIPiDIP-1564N.
IntActiP33400. 10 interactors.
MINTiMINT-386832.

PTM databases

iPTMnetiP33400.

Proteomic databases

MaxQBiP33400.
PRIDEiP33400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL027W; YHL027W; YHL027W.
GeneIDi856358.
KEGGisce:YHL027W.

Organism-specific databases

EuPathDBiFungiDB:YHL027W.
SGDiS000001019. RIM101.

Phylogenomic databases

InParanoidiP33400.
OrthoDBiEOG092C1EDI.

Enzyme and pathway databases

BioCyciYEAST:G3O-31047-MONOMER.

Miscellaneous databases

PROiP33400.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPACC_YEAST
AccessioniPrimary (citable) accession number: P33400
Secondary accession number(s): D3DKU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1822 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.