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P33400

- PACC_YEAST

UniProt

P33400 - PACC_YEAST

Protein

pH-response transcription factor pacC/RIM101

Gene

RIM101

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri146 – 17126C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri182 – 20625C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri212 – 23423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. barrier septum assembly Source: SGD
    3. cellular response to alkaline pH Source: SGD
    4. cellular response to anoxia Source: SGD
    5. fungal-type cell wall biogenesis Source: SGD
    6. meiotic nuclear division Source: SGD
    7. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    8. positive regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Meiosis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31047-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    pH-response transcription factor pacC/RIM101
    Alternative name(s):
    Regulator of IME2 protein 1
    pH-response regulator protein RIM101
    Gene namesi
    Name:RIM101
    Synonyms:RIM1
    Ordered Locus Names:YHL027W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHL027w.
    SGDiS000001019. RIM101.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi153 – 1531C → S: Abolishes transcriptional activation of target genes. 1 Publication
    Mutagenesisi189 – 1891C → S: Abolishes transcriptional activation of target genes. 1 Publication
    Mutagenesisi217 – 2171C → S: Abolishes transcriptional activation of target genes. 1 Publication
    Mutagenesisi255 – 2551S → A: Reduces transcriptional activation of target genes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625pH-response transcription factor pacC/RIM101PRO_0000046847Add
    BLAST

    Post-translational modificationi

    Activated by C-terminal proteolytic cleavage. At neutral to alkaline ambient pH, the signaling protease (probably RIM13) cleaves RIM101, removing a C-terminal 8 kDa peptide to yield the active form.1 Publication

    Proteomic databases

    MaxQBiP33400.
    PaxDbiP33400.

    Expressioni

    Gene expression databases

    GenevestigatoriP33400.

    Interactioni

    Subunit structurei

    Binds to DNA. Interacts with RIM20, which probably binds to the two YPX[LI] motifs and is required for proteolytic processing.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LSB3P436032EBI-14422,EBI-22980
    RIM20Q120333EBI-14422,EBI-38947
    YSC84P327932EBI-14422,EBI-24460

    Protein-protein interaction databases

    BioGridi36395. 365 interactions.
    DIPiDIP-1564N.
    IntActiP33400. 9 interactions.
    MINTiMINT-386832.
    STRINGi4932.YHL027W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33400.
    SMRiP33400. Positions 148-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi228 – 2347Nuclear localization signalBy similarity
    Motifi450 – 4534YPX[LI] motif 1
    Motifi620 – 6234YPX[LI] motif 2

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi76 – 12045Ser-richAdd
    BLAST
    Compositional biasi131 – 14010Poly-Asp
    Compositional biasi579 – 60123Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the pacC/RIM101 family.Curated
    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri146 – 17126C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri182 – 20625C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri212 – 23423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG245897.
    OMAiTIANATQ.
    OrthoDBiEOG75F4P2.

    Family and domain databases

    Gene3Di3.30.160.60. 1 hit.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33400-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPLEDLLNK ENGTAAPQHS RESIVENGTD VSNVTKKDGL PSPNLSKRSS    50
    DCSKRPRIRC TTEAIGLNGQ EDERMSPGST SSSCLPYHST SHLNTPPYDL 100
    LGASAVSPTT SSSSDSSSSS PLAQAHNPAG DDDDADNDGD SEDITLYCKW 150
    DNCGMIFNQP ELLYNHLCHD HVGRKSHKNL QLNCHWGDCT TKTEKRDHIT 200
    SHLRVHVPLK PFGCSTCSKK FKRPQDLKKH LKIHLESGGI LKRKRGPKWG 250
    SKRTSKKNKS CASDAVSSCS ASVPSAIAGS FKSHSTSPQI LPPLPVGISQ 300
    HLPSQQQQRA ISLNQLCSDE LSQYKPVYSP QLSARLQTIL PPLYYNNGST 350
    VSQGANSRSM NVYEDGCSNK TIANATQFFT KLSRNMTNNY ILQQSGGSTE 400
    SSSSSGRIPV AQTSYVQPPN APSYQSVQGG SSISATANTA TYVPVRLAKY 450
    PTGPSLTEHL PPLHSNTAGG VFNRQSQYAM PHYPSVRAAP SYSSSGCSIL 500
    PPLQSKIPML PSRRTMAGGT SLKPNWEFSL NQKSCTNDII MSKLAIEEVD 550
    DESEIEDDFV EMLGIVNIIK DYLLCCVMED LDDEESEDKD EENAFLQESL 600
    EKLSLQNQMG TNSVRILTKY PKILV 625
    Length:625
    Mass (Da):68,232
    Last modified:February 1, 1995 - v2
    Checksum:iAD71E7B2D679E17E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681N → K in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti90 – 901T → S in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti111 – 1111S → P in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti249 – 2491W → L in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti261 – 2611C → S in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti276 – 2761A → G in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti308 – 3081Q → QQQQ(PubMed:8367297)Curated
    Sequence conflicti358 – 3581R → Q in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti486 – 4861V → I in CAA51462. (PubMed:8367297)Curated
    Sequence conflicti519 – 5191G → E in CAA51462. (PubMed:8367297)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72960 Genomic DNA. Translation: CAA51462.1.
    U11583 Genomic DNA. Translation: AAB65039.1.
    AY693138 Genomic DNA. Translation: AAT93157.1.
    BK006934 Genomic DNA. Translation: DAA06658.1.
    PIRiS48941.
    RefSeqiNP_011836.1. NM_001179107.1.

    Genome annotation databases

    EnsemblFungiiYHL027W; YHL027W; YHL027W.
    GeneIDi856358.
    KEGGisce:YHL027W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72960 Genomic DNA. Translation: CAA51462.1 .
    U11583 Genomic DNA. Translation: AAB65039.1 .
    AY693138 Genomic DNA. Translation: AAT93157.1 .
    BK006934 Genomic DNA. Translation: DAA06658.1 .
    PIRi S48941.
    RefSeqi NP_011836.1. NM_001179107.1.

    3D structure databases

    ProteinModelPortali P33400.
    SMRi P33400. Positions 148-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36395. 365 interactions.
    DIPi DIP-1564N.
    IntActi P33400. 9 interactions.
    MINTi MINT-386832.
    STRINGi 4932.YHL027W.

    Proteomic databases

    MaxQBi P33400.
    PaxDbi P33400.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL027W ; YHL027W ; YHL027W .
    GeneIDi 856358.
    KEGGi sce:YHL027W.

    Organism-specific databases

    CYGDi YHL027w.
    SGDi S000001019. RIM101.

    Phylogenomic databases

    eggNOGi NOG245897.
    OMAi TIANATQ.
    OrthoDBi EOG75F4P2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31047-MONOMER.

    Miscellaneous databases

    NextBioi 981811.

    Gene expression databases

    Genevestigatori P33400.

    Family and domain databases

    Gene3Di 3.30.160.60. 1 hit.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the yeast meiotic regulatory gene RIM1."
      Su S.S.Y., Mitchell A.P.
      Nucleic Acids Res. 21:3789-3797(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-153; CYS-189; CYS-217 AND SER-255.
      Strain: SK1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth."
      Li W., Mitchell A.P.
      Genetics 145:63-73(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING.
    6. "Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway."
      Lamb T.M., Xu W., Diamond A., Mitchell A.P.
      J. Biol. Chem. 276:1850-1856(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage."
      Xu W., Mitchell A.P.
      J. Bacteriol. 183:6917-6923(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RIM20.
    8. "The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae."
      Lamb T.M., Mitchell A.P.
      Mol. Cell. Biol. 23:677-686(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION, DNA-BINDING.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPACC_YEAST
    AccessioniPrimary (citable) accession number: P33400
    Secondary accession number(s): D3DKU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1822 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3