Reviewed,
UniProtKB/Swiss-Prot P33400 (PACC_YEAST)
Last modified
December 15, 2009.
Version 90.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: pH-response transcription factor pacC/RIM101 Alternative name(s): pH-response regulator protein RIM101 Regulator of IME2 protein 1 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 625 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth. Ref.5 Ref.7 |
| Subunit structure | Binds to DNA. Interacts with RIM20, which probably binds to the two YPX[LI] motifs and is required for proteolytic processing. Ref.6 |
| Subcellular location | |
| Post-translational modification | Activated by C-terminal proteolytic cleavage. At neutral to alkaline ambient pH, the signaling protease (probably RIM13) cleaves RIM101, removing a C-terminal 8 kDa peptide to yield the active form. |
| Miscellaneous | Present with 1822 molecules/cell in log phase SD medium. Ref.9 |
| Sequence similarities | Belongs to the pacC/RIM101 family. Contains 3 C2H2-type zinc fingers. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| LSB3 | P43603 | 1 | EBI-14422,EBI-22980 | |
| RIM20 | Q12033 | 1 | EBI-14422,EBI-38947 | |
| RVS167 | P39743 | 1 | EBI-14422,EBI-14500 | |
| YSC84 | P32793 | 1 | EBI-14422,EBI-24460 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 625 | 625 | pH-response transcription factor pacC/RIM101 | PRO_0000046847 | |||||
Regions | |||||||||
| Zinc finger | 146 – 171 | 26 | C2H2-type 1 | ||||||
| Zinc finger | 182 – 206 | 25 | C2H2-type 2 | ||||||
| Zinc finger | 212 – 234 | 23 | C2H2-type 3 | ||||||
| Motif | 228 – 234 | 7 | Nuclear localization signal By similarity | ||||||
| Motif | 450 – 453 | 4 | YPX[LI] motif 1 | ||||||
| Motif | 620 – 623 | 4 | YPX[LI] motif 2 | ||||||
| Compositional bias | 76 – 120 | 45 | Ser-rich | ||||||
| Compositional bias | 131 – 140 | 10 | Poly-Asp | ||||||
| Compositional bias | 579 – 601 | 23 | Asp/Glu-rich (acidic) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 42 | 1 | Phosphoserine Ref.10 | ||||||
Experimental info | |||||||||
| Mutagenesis | 153 | 1 | C → S: Abolishes transcriptional activation of target genes. Ref.1 | ||||||
| Mutagenesis | 189 | 1 | C → S: Abolishes transcriptional activation of target genes. Ref.1 | ||||||
| Mutagenesis | 217 | 1 | C → S: Abolishes transcriptional activation of target genes. Ref.1 | ||||||
| Mutagenesis | 255 | 1 | S → A: Reduces transcriptional activation of target genes. Ref.1 | ||||||
| Sequence conflict | 68 | 1 | N → K in CAA51462. Ref.1 | ||||||
| Sequence conflict | 90 | 1 | T → S in CAA51462. Ref.1 | ||||||
| Sequence conflict | 111 | 1 | S → P in CAA51462. Ref.1 | ||||||
| Sequence conflict | 249 | 1 | W → L in CAA51462. Ref.1 | ||||||
| Sequence conflict | 261 | 1 | C → S in CAA51462. Ref.1 | ||||||
| Sequence conflict | 276 | 1 | A → G in CAA51462. Ref.1 | ||||||
| Sequence conflict | 308 | 1 | Q → QQQQ Ref.1 | ||||||
| Sequence conflict | 358 | 1 | R → Q in CAA51462. Ref.1 | ||||||
| Sequence conflict | 486 | 1 | V → I in CAA51462. Ref.1 | ||||||
| Sequence conflict | 519 | 1 | G → E in CAA51462. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of the yeast meiotic regulatory gene RIM1." Su S.S.Y., Mitchell A.P. Nucleic Acids Res. 21:3789-3797(1993) [PubMed: 8367297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-153; CYS-189; CYS-217 AND SER-255. Strain: SK1. |
| [2] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M.Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth." Li W., Mitchell A.P. Genetics 145:63-73(1997) [PubMed: 9017390] [Abstract] Cited for: PROTEOLYTIC PROCESSING. |
| [5] | "Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway." Lamb T.M., Xu W., Diamond A., Mitchell A.P. J. Biol. Chem. 276:1850-1856(2001) [PubMed: 11050096] [Abstract] Cited for: FUNCTION. |
| [6] | "Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage." Xu W., Mitchell A.P. J. Bacteriol. 183:6917-6923(2001) [PubMed: 11698381] [Abstract] Cited for: INTERACTION WITH RIM20. |
| [7] | "The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae." Lamb T.M., Mitchell A.P. Mol. Cell. Biol. 23:677-686(2003) [PubMed: 12509465] [Abstract] Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION, DNA-BINDING. |
| [8] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [9] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X72960 Genomic DNA. Translation: CAA51462.1. U11583 Genomic DNA. Translation: AAB65039.1. AY693138 Genomic DNA. Translation: AAT93157.1. | |
| PIR | S48941. |
| RefSeq | NP_011836.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-1564N. |
| IntAct | P33400. 12 interactions. |
| STRING | P33400. |
Genome annotation databases | |
| Ensembl | YHL027W; YHL027W; YHL027W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856358. |
| KEGG | sce:YHL027W. |
| NMPDR | fig|4932.3.peg.2974. |
Organism-specific databases | |
| CYGD | YHL027w. |
| SGD | S000001019. RIM101. |
Phylogenomic databases | |
| OrthoDB | EOG96HHTC. |
Gene expression databases | |
| ArrayExpress | P33400. |
| Genevestigator | P33400. |
| GermOnline | YHL027W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit. |
| Pfam | PF00096. zf-C2H2. 2 hits. [Graphical view] |
| SMART | SM00355. ZnF_C2H2. 3 hits. [Graphical view] |
| PROSITE | PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 981811. |
Entry information
| Entry name | PACC_YEAST | ||||||||
| Accession | Primary (citable) accession number: P33400 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VIII Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names |

Clusters with


