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Protein

La protein homolog

Gene

LHP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone that binds nascent RNA polymerase III transcripts, stabilizing these RNAs against exonucleases. Required for the 3' endonucleolytic cleavage that matures tRNA precursors and for efficient folding of certain pre-tRNAs. Cooperaes with GCD14 in the maturation of a subset of RNA polymerase III transcripts. Functions also in the assembly of certain RNA polymerase II-transcribed RNAs into RNPs. Binds and stabilizes newly synthesized U6 snRNA as well as precursors of U3 snRNA from degradation. Facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP. Required fot Ty3 normal transposition frequency.16 Publications

GO - Molecular functioni

  • RNA binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

  • tRNA processing Source: SGD

Keywordsi

Molecular functionRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29469-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
La protein homolog
Alternative name(s):
La autoantigen homolog
La ribonucleoprotein
Gene namesi
Name:LHP1
Synonyms:LAH1, YLA1
Ordered Locus Names:YDL051W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL051W.
SGDiS000002209. LHP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15S → A: Prevents phosphorylation; when associated with A-19 and A-235. 1 Publication1
Mutagenesisi19S → A: Prevents phosphorylation; when associated with A-15 and A-235. 1 Publication1
Mutagenesisi235S → A: Prevents phosphorylation; when associated with A-15 and A-19. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002076082 – 275La protein homologAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei15PhosphoserineCombined sources1 Publication1
Modified residuei19PhosphoserineCombined sources1 Publication1
Modified residuei104Omega-N-methylarginine1 Publication1
Modified residuei230PhosphoserineCombined sources1
Modified residuei233PhosphoserineCombined sources1
Modified residuei235PhosphoserineCombined sources1 Publication1

Post-translational modificationi

Phosphorylation is not required for the roles tRNA and snRNP biogenesis.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP33399.
PRIDEiP33399.

PTM databases

iPTMnetiP33399.

Interactioni

Subunit structurei

Interacts with SXM1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332032EBI-10046,EBI-701

Protein-protein interaction databases

BioGridi32008. 140 interactors.
DIPiDIP-6444N.
IntActiP33399. 20 interactors.
MINTiMINT-652314.
STRINGi4932.YDL051W.

Structurei

3D structure databases

ProteinModelPortaliP33399.
SMRiP33399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 112HTH La-type RNA-bindingPROSITE-ProRule annotationAdd BLAST90
Domaini123 – 216RRMPROSITE-ProRule annotationAdd BLAST94

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi240 – 256Nuclear localization signalSequence analysisAdd BLAST17

Phylogenomic databases

GeneTreeiENSGT00830000128380.
InParanoidiP33399.
KOiK11090.
OMAiNDGWVEL.
OrthoDBiEOG092C4I3J.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR006630. La_HTH.
IPR002344. Lupus_La.
IPR035979. RBD_domain_sf.
IPR000504. RRM_dom.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
PRINTSiPR00302. LUPUSLA.
SMARTiView protein in SMART
SM00715. LA. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKPQQEEQ EKPQSRRNSF AVIEFTPEVL DRCLKQVEFY FSEFNFPYDR
60 70 80 90 100
FLRTTAEKND GWVPISTIAT FNRMKKYRPV DKVIEALRSS EILEVSADGE
110 120 130 140 150
NVKRRVPLDL TAARNARIEQ NQRTLAVMNF PHEDVEASQI PELQENLEAF
160 170 180 190 200
FKKLGEINQV RLRRDHRNKK FNGTVLVEFK TIPECEAFLK SYSNDDESNE
210 220 230 240 250
ILSYEGKKLS VLTKKQFDLQ REASKSKNFS GRSRSFNGHK KKNLPKFPKN
260 270
KKKNGKEESK EDSSAIADDD EEHKE
Length:275
Mass (Da):32,104
Last modified:October 1, 1994 - v2
Checksum:i43CDB2E6C740978D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33023 Genomic DNA. Translation: AAA21777.1.
Z74099 Genomic DNA. Translation: CAA98612.1.
AY558373 Genomic DNA. Translation: AAS56699.1.
L13282 Unassigned DNA. Translation: AAA16515.1.
X80801 Genomic DNA. Translation: CAA56782.1.
BK006938 Genomic DNA. Translation: DAA11805.1.
PIRiB48600.
RefSeqiNP_010232.1. NM_001180110.1.

Genome annotation databases

EnsemblFungiiYDL051W; YDL051W; YDL051W.
GeneIDi851509.
KEGGisce:YDL051W.

Similar proteinsi

Entry informationi

Entry nameiLHP1_YEAST
AccessioniPrimary (citable) accession number: P33399
Secondary accession number(s): D6VRU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names