P33399 (LHP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
La protein homolog

Alternative name(s):
La autoantigen homolog
La ribonucleoprotein

Gene names

Name:	LHP1
Synonyms:	LAH1, YLA1
Ordered Locus Names:	YDL051W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone that binds nascent RNA polymerase III transcripts, stabilizing these RNAs against exonucleases. Required for the 3' endonucleolytic cleavage that matures tRNA precursors and for efficient folding of certain pre-tRNAs. Cooperaes with GCD14 in the maturation of a subset of RNA polymerase III transcripts. Functions also in the assembly of certain RNA polymerase II-transcribed RNAs into RNPs. Binds and stabilizes newly synthesized U6 snRNA as well as precursors of U3 snRNA from degradation. Facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP. Required fot Ty3 normal transposition frequency. Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.24 Ref.26
Subunit structure	Interacts with SXM1. Ref.8 Ref.11
Subcellular location	Nucleus. Note: Imported into nucleus by the nuclear transport factor SXM1. Ref.8 Ref.11 Ref.13
Post-translational modification	Phosphorylation is not required for the roles tRNA and snRNP biogenesis.
Sequence similarities	Contains 1 HTH La-type RNA-binding domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.18. Source: SGD nucleoplasm Inferred from direct assay Ref.18. Source: SGD ribonucleoprotein complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from direct assay PubMed 19749380. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	2	EBI-10046,EBI-701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	La protein homolog		PRO_0000207608
Regions
	Domain	23 – 112	90	HTH La-type RNA-binding
	Domain	123 – 216	94	RRM
	Motif	240 – 256	17	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.18 Ref.28 Ref.30
	Modified residue	19	1	Phosphoserine Ref.18 Ref.25 Ref.27 Ref.28 Ref.29 Ref.30
	Modified residue	230	1	Phosphoserine Ref.28
	Modified residue	233	1	Phosphoserine Ref.28
	Modified residue	235	1	Phosphoserine Ref.18 Ref.28
Experimental info
	Mutagenesis	15	1	S → A: Prevents phosphorylation; when associated with A-19 and A-235. Ref.18
	Mutagenesis	19	1	S → A: Prevents phosphorylation; when associated with A-15 and A-235. Ref.18
	Mutagenesis	235	1	S → A: Prevents phosphorylation; when associated with A-15 and A-19. Ref.18

Sequences

	Sequence		Length	Mass (Da)
	P33399 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 43CDB2E6C740978D	FASTA	275	32,104
	10 20 30 40 50 60 MSEKPQQEEQ EKPQSRRNSF AVIEFTPEVL DRCLKQVEFY FSEFNFPYDR FLRTTAEKND 70 80 90 100 110 120 GWVPISTIAT FNRMKKYRPV DKVIEALRSS EILEVSADGE NVKRRVPLDL TAARNARIEQ 130 140 150 160 170 180 NQRTLAVMNF PHEDVEASQI PELQENLEAF FKKLGEINQV RLRRDHRNKK FNGTVLVEFK 190 200 210 220 230 240 TIPECEAFLK SYSNDDESNE ILSYEGKKLS VLTKKQFDLQ REASKSKNFS GRSRSFNGHK 250 260 270 KKNLPKFPKN KKKNGKEESK EDSSAIADDD EEHKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a yeast homolog of the La autoantigen is dispensable for growth." Yoo C.J., Wolin S.L. Mol. Cell. Biol. 14:5412-5424(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: YPH501.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"A yeast RNA binding protein that resembles the human autoantigen La." Lin-Marq N., Clarkson S.G. J. Mol. Biol. 245:81-85(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 101-275, CHARACTERIZATION.
[6]	"A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase." Nagiec M.M., Wells G.B., Lester R.L., Dickson R.C. J. Biol. Chem. 268:22156-22163(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-252.
[7]	"The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors." Yoo C.J., Wolin S.L. Cell 89:393-402(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"A nuclear import pathway for a protein involved in tRNA maturation." Rosenblum J.S., Pemberton L.F., Blobel G. J. Cell Biol. 139:1655-1661(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SXM1, FUNCTION, SUBCELLULAR LOCATION.
[9]	"A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts." Pannone B.K., Xue D., Wolin S.L. EMBO J. 17:7442-7453(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, U6 RNA-BINDING.
[10]	"The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA." Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G. Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Nuclear import and the evolution of a multifunctional RNA-binding protein." Rosenblum J.S., Pemberton L.F., Bonifaci N., Blobel G. J. Cell Biol. 143:887-899(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SXM1, SUBCELLULAR LOCATION.
[12]	"Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway." Dechampesme A.M., Koroleva O., Leger-Silvestre I., Gas N., Camier S. J. Cell Biol. 145:1369-1380(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[13]	"Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes." Sobel S.G., Wolin S.L. Mol. Biol. Cell 10:3849-3862(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[14]	"GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae." Calvo O., Cuesta R., Anderson J., Gutierrez N., Garcia-Barrio M.T., Hinnebusch A.G., Tamame M. Mol. Cell. Biol. 19:4167-4181(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"U snRNP assembly in yeast involves the La protein." Xue D., Rubinson D.A., Pannone B.K., Yoo C.J., Wolin S.L. EMBO J. 19:1650-1660(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[16]	"Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding." Kufel J., Allmang C., Chanfreau G., Petfalski E., Lafontaine D.L., Tollervey D. Mol. Cell. Biol. 20:5415-5424(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[17]	"Multiple functional interactions between components of the Lsm2-Lsm8 complex, U6 snRNA, and the yeast La protein." Pannone B.K., Kim S.D., Noe D.A., Wolin S.L. Genetics 158:187-196(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[18]	"Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization." Long K.S., Cedervall T., Walch-Solimena C., Noe D.A., Huddleston M.J., Annan R.S., Wolin S.L. RNA 7:1589-1602(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-15; SER-19 AND SER-235, MASS SPECTROMETRY, FUNCTION, MUTAGENESIS OF SER-15; SER-19 AND SER-235.
[19]	"Lsm proteins are required for normal processing of pre-tRNAs and their efficient association with La-homologous protein Lhp1p." Kufel J., Allmang C., Verdone L., Beggs J.D., Tollervey D. Mol. Cell. Biol. 22:5248-5256(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[20]	"A La protein requirement for efficient pre-tRNA folding." Chakshusmathi G., Kim S.D., Rubinson D.A., Wolin S.L. EMBO J. 22:6562-6572(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[21]	"Ty3 requires yeast La homologous protein for wild-type frequencies of transposition." Aye M., Sandmeyer S.B. Mol. Microbiol. 49:501-515(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[22]	"A complex pathway for 3' processing of the yeast U3 snoRNA." Kufel J., Allmang C., Verdone L., Beggs J., Tollervey D. Nucleic Acids Res. 31:6788-6797(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[23]	"Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs." Inada M., Guthrie C. Proc. Natl. Acad. Sci. U.S.A. 101:434-439(2004) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING.
[24]	"Host factors that affect Ty3 retrotransposition in Saccharomyces cerevisiae." Aye M., Irwin B., Beliakova-Bethell N., Chen E., Garrus J., Sandmeyer S. Genetics 168:1159-1176(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[25]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Strain: YAL6B.
[26]	"The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability." Copela L.A., Chakshusmathi G., Sherrer R.L., Wolin S.L. RNA 12:644-654(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[27]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Strain: ADR376.
[28]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-19; SER-230; SER-233 AND SER-235, MASS SPECTROMETRY.
[29]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY.
[30]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-19, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33023 Genomic DNA. Translation: AAA21777.1. Z74099 Genomic DNA. Translation: CAA98612.1. AY558373 Genomic DNA. Translation: AAS56699.1. L13282 Unassigned DNA. Translation: AAA16515.1. X80801 Genomic DNA. Translation: CAA56782.1. BK006938 Genomic DNA. Translation: DAA11805.1.
PIR	B48600.
RefSeq	NP_010232.1. NM_001180110.1.
3D structure databases
ProteinModelPortal	P33399.
SMR	P33399. Positions 35-202.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6444N.
IntAct	P33399. 20 interactions.
MINT	MINT-652314.
STRING	4932.YDL051W.
Proteomic databases
PaxDb	P33399.
PeptideAtlas	P33399.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL051W; YDL051W; YDL051W.
GeneID	851509.
KEGG	sce:YDL051W.
Organism-specific databases
CYGD	YDL051w.
SGD	S000002209. LHP1.
Phylogenomic databases
eggNOG	COG5193.
GeneTree	ENSGT00530000063464.
KO	K11090.
OMA	NDGWVEL.
OrthoDB	EOG43JGF6.
Gene expression databases
Genevestigator	P33399.
GermOnline	YDL051W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 3.30.70.330. 1 hit.
InterPro	IPR002344. Lupus_La. IPR006630. Lupus_La_RNA-bd. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF05383. La. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
PRINTS	PR00302. LUPUSLA.
SMART	SM00715. LA. 1 hit. SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50961. HTH_LA. 1 hit. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968869.

Entry information

Entry name

LHP1_YEAST

Accession

Primary (citable) accession number: P33399
Secondary accession number(s): D6VRU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program