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Protein

La protein homolog

Gene

LHP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone that binds nascent RNA polymerase III transcripts, stabilizing these RNAs against exonucleases. Required for the 3' endonucleolytic cleavage that matures tRNA precursors and for efficient folding of certain pre-tRNAs. Cooperaes with GCD14 in the maturation of a subset of RNA polymerase III transcripts. Functions also in the assembly of certain RNA polymerase II-transcribed RNAs into RNPs. Binds and stabilizes newly synthesized U6 snRNA as well as precursors of U3 snRNA from degradation. Facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP. Required fot Ty3 normal transposition frequency.16 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

  • tRNA processing Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29469-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
La protein homolog
Alternative name(s):
La autoantigen homolog
La ribonucleoprotein
Gene namesi
Name:LHP1
Synonyms:LAH1, YLA1
Ordered Locus Names:YDL051W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL051W.
SGDiS000002209. LHP1.

Subcellular locationi

GO - Cellular componenti

  • intracellular ribonucleoprotein complex Source: InterPro
  • nucleolus Source: SGD
  • nucleoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15S → A: Prevents phosphorylation; when associated with A-19 and A-235. 1 Publication1
Mutagenesisi19S → A: Prevents phosphorylation; when associated with A-15 and A-235. 1 Publication1
Mutagenesisi235S → A: Prevents phosphorylation; when associated with A-15 and A-19. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002076082 – 275La protein homologAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei15PhosphoserineCombined sources1 Publication1
Modified residuei19PhosphoserineCombined sources1 Publication1
Modified residuei104Omega-N-methylarginine1 Publication1
Modified residuei230PhosphoserineCombined sources1
Modified residuei233PhosphoserineCombined sources1
Modified residuei235PhosphoserineCombined sources1 Publication1

Post-translational modificationi

Phosphorylation is not required for the roles tRNA and snRNP biogenesis.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP33399.
PRIDEiP33399.

PTM databases

iPTMnetiP33399.

Interactioni

Subunit structurei

Interacts with SXM1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332032EBI-10046,EBI-701

Protein-protein interaction databases

BioGridi32008. 96 interactors.
DIPiDIP-6444N.
IntActiP33399. 19 interactors.
MINTiMINT-652314.

Structurei

3D structure databases

ProteinModelPortaliP33399.
SMRiP33399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 112HTH La-type RNA-bindingPROSITE-ProRule annotationAdd BLAST90
Domaini123 – 216RRMPROSITE-ProRule annotationAdd BLAST94

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi240 – 256Nuclear localization signalSequence analysisAdd BLAST17

Sequence similaritiesi

Contains 1 HTH La-type RNA-binding domain.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00830000128380.
InParanoidiP33399.
KOiK11090.
OMAiILTFGRM.
OrthoDBiEOG092C4I3J.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002344. Lupus_La.
IPR006630. Lupus_La_RNA-bd.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR00302. LUPUSLA.
SMARTiSM00715. LA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKPQQEEQ EKPQSRRNSF AVIEFTPEVL DRCLKQVEFY FSEFNFPYDR
60 70 80 90 100
FLRTTAEKND GWVPISTIAT FNRMKKYRPV DKVIEALRSS EILEVSADGE
110 120 130 140 150
NVKRRVPLDL TAARNARIEQ NQRTLAVMNF PHEDVEASQI PELQENLEAF
160 170 180 190 200
FKKLGEINQV RLRRDHRNKK FNGTVLVEFK TIPECEAFLK SYSNDDESNE
210 220 230 240 250
ILSYEGKKLS VLTKKQFDLQ REASKSKNFS GRSRSFNGHK KKNLPKFPKN
260 270
KKKNGKEESK EDSSAIADDD EEHKE
Length:275
Mass (Da):32,104
Last modified:October 1, 1994 - v2
Checksum:i43CDB2E6C740978D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33023 Genomic DNA. Translation: AAA21777.1.
Z74099 Genomic DNA. Translation: CAA98612.1.
AY558373 Genomic DNA. Translation: AAS56699.1.
L13282 Unassigned DNA. Translation: AAA16515.1.
X80801 Genomic DNA. Translation: CAA56782.1.
BK006938 Genomic DNA. Translation: DAA11805.1.
PIRiB48600.
RefSeqiNP_010232.1. NM_001180110.1.

Genome annotation databases

EnsemblFungiiYDL051W; YDL051W; YDL051W.
GeneIDi851509.
KEGGisce:YDL051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33023 Genomic DNA. Translation: AAA21777.1.
Z74099 Genomic DNA. Translation: CAA98612.1.
AY558373 Genomic DNA. Translation: AAS56699.1.
L13282 Unassigned DNA. Translation: AAA16515.1.
X80801 Genomic DNA. Translation: CAA56782.1.
BK006938 Genomic DNA. Translation: DAA11805.1.
PIRiB48600.
RefSeqiNP_010232.1. NM_001180110.1.

3D structure databases

ProteinModelPortaliP33399.
SMRiP33399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32008. 96 interactors.
DIPiDIP-6444N.
IntActiP33399. 19 interactors.
MINTiMINT-652314.

PTM databases

iPTMnetiP33399.

Proteomic databases

MaxQBiP33399.
PRIDEiP33399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL051W; YDL051W; YDL051W.
GeneIDi851509.
KEGGisce:YDL051W.

Organism-specific databases

EuPathDBiFungiDB:YDL051W.
SGDiS000002209. LHP1.

Phylogenomic databases

GeneTreeiENSGT00830000128380.
InParanoidiP33399.
KOiK11090.
OMAiILTFGRM.
OrthoDBiEOG092C4I3J.

Enzyme and pathway databases

BioCyciYEAST:G3O-29469-MONOMER.

Miscellaneous databases

PROiP33399.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002344. Lupus_La.
IPR006630. Lupus_La_RNA-bd.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR00302. LUPUSLA.
SMARTiSM00715. LA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLHP1_YEAST
AccessioniPrimary (citable) accession number: P33399
Secondary accession number(s): D6VRU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.