Reviewed, UniProtKB/Swiss-Prot P33380 (LDH1_LISMO)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldh1
Synonyms:	ldh
Ordered Locus Names:	lmo0210

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000168365
Regions
	Nucleotide binding	13 – 41	29	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	121	1	NAD or substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
Amino acid modifications
	Modified residue	218	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33380-1 [UniParc]. Last modified November 16, 2001. Version 2. Checksum: B05D3AEFAAADBBB1	FASTA	313	34,192
	10 20 30 40 50 60 MKDHQKIILV GDGAVGSSYA FACVNLSIGQ EFGIIDIDKD RTIGDAMDLS HAVPFSTPKK 70 80 90 100 110 120 IYSANYSDCH DADLVVVTAG TAQKPGETRL DLVNRNIKIM KGIVDEVMAS GFDGIFLIAS 130 140 150 160 170 180 NPVDILTYAT WKFSGLPKER VIGSGTSLDT ARFRMSIADY LKVDARNVHG YILGEHGDTE 190 200 210 220 230 240 FPAWSHTTVG GLPITEWISE DEQGAMDTIF VSVRDAAYEI INKKGATFYG VAAALARITK 250 260 270 280 290 300 AILNNENAIL PLSVYLDGHY GMNDIYIGAP AVVNRQGVRH IVEMNLNDKE KEQMKNSADT 310 LKKVLDDAMK QID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
[2]	"Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread." Vazquez-Boland J.-A., Kocks C., Dramsi S., Ohayon H., Geoffroy C., Mengaud J., Cossart P. Infect. Immun. 60:219-230(1992) [PubMed: 1309513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 180-313. Strain: LO28 / Serovar 1/2c.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591974 Genomic DNA. Translation: CAD00737.1. M82881 Genomic DNA. Translation: AAA25274.1.
PIR	AC1101. G43868.
RefSeq	NP_463741.1. YP_002756946.1.
3D structure databases
HSSP	HSSP built from PDB template 2LDB based on UniProtKB P00344.
ModBase	Search...
Genome annotation databases
GeneID	7703645. 987043.
GenomeReviews	Gene locus lmo0210 in contig AL591824_GR.
KEGG	lmo:lmo0210.
Organism-specific databases
ListiList	LMO00210.
CMR	Search...
Phylogenomic databases
HOGENOM	P33380.
OMA	P33380. WINEDEQ.
Enzyme and pathway databases
BioCyc	LMON169963:LMO0210-MON.
BRENDA	1.1.1.27. 96770.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_LISMO

Accession

Primary (citable) accession number: P33380

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 16, 2001
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)