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Protein

Actin assembly-inducing protein

Gene

actA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Virulence factor required for host cell microfilament interaction. It induces actin assembly around the bacteria to allow it to move within the cytoplasm. It is involved in the actin polymerization process. It seems to act as a nucleator that induces the reorganization of the actin cytoskeleton.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Actin assembly-inducing protein
Gene namesi
Name:actA
Synonyms:prtB
Ordered Locus Names:lmo0204
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei613 – 633HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000002062530 – 639Actin assembly-inducing proteinAdd BLAST610

Proteomic databases

PaxDbiP33379.

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0204.

Structurei

3D structure databases

ProteinModelPortaliP33379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati264 – 2981Add BLAST35
Repeati299 – 3332Add BLAST35
Repeati334 – 3783; approximateAdd BLAST45
Repeati379 – 4174; approximateAdd BLAST39
Repeati418 – 4225; truncated5

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 3335 X approximate tandem repeats, Pro-richAdd BLAST70

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi360 – 362Cell attachment siteSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi266 – 339Pro-richAdd BLAST74

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000033794.
KOiK16644.
OMAiSLTYPDK.

Family and domain databases

InterProiIPR007752. Virulence_actor_ActA.
[Graphical view]
PfamiPF05058. ActA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLNRFMRAM MVVFITANCI TINPDIIFAA TDSEDSSLNT DEWEEEKTEE
60 70 80 90 100
QPSEVNTGPR YETAREVSSR DIKELEKSNK VRNTNKADLI AMLKEKAEKG
110 120 130 140 150
PNINNNNSEQ TENAAINEEA SGADRPAIQV ERRHPGLPSD SAAEIKKRRK
160 170 180 190 200
AIASSDSELE SLTYPDKPTK VNKKKVAKES VADASESDLD SSMQSADESS
210 220 230 240 250
PQPLKANQQP FFPKVFKKIK DAGKWVRDKI DENPEVKKAI VDKSAGLIDQ
260 270 280 290 300
LLTKKKSEEV NASDFPPPPT DEELRLALPE TPMLLGFNAP ATSEPSSFEF
310 320 330 340 350
PPPPTDEELR LALPETPMLL GFNAPATSEP SSFEFPPPPT EDELEIIRET
360 370 380 390 400
ASSLDSSFTR GDLASLRNAI NRHSQNFSDF PPIPTEEELN GRGGRPTSEE
410 420 430 440 450
FSSLNSGDFT DDENSETTEE EIDRLADLRD RGTGKHSRNA GFLPLNPFAS
460 470 480 490 500
SPVPSLSPKV SKISAPALIS DITKKTPFKN PSQPLNVFNK KTTTKTVTKK
510 520 530 540 550
PTPVKTAPKL AELPATKPQE TVLRENKTPF IEKQAETNKQ SINMPSLPVI
560 570 580 590 600
QKEATESDKE EMKPQTEEKM VEESESANNA NGKNRSAGIE EGKLIAKSAE
610 620 630
DEKAKEEPGN HTTLILAMLA IGVFSLGAFI KIIQLRKNN
Length:639
Mass (Da):70,349
Last modified:February 1, 1994 - v1
Checksum:i5A06CF78BC5F3C91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465 – 466AP → DR in CAA42407 (PubMed:1582425).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82881 Genomic DNA. Translation: AAA25269.1.
X59723 Genomic DNA. Translation: CAA42407.1.
AL591974 Genomic DNA. Translation: CAD00731.1.
PIRiAE1100.
S20887.
RefSeqiNP_463735.1. NC_003210.1.
WP_010989374.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00731; CAD00731; CAD00731.
GeneIDi987035.
KEGGilmo:lmo0204.
PATRICi20309415. VBILisMon69206_0209.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82881 Genomic DNA. Translation: AAA25269.1.
X59723 Genomic DNA. Translation: CAA42407.1.
AL591974 Genomic DNA. Translation: CAD00731.1.
PIRiAE1100.
S20887.
RefSeqiNP_463735.1. NC_003210.1.
WP_010989374.1. NC_003210.1.

3D structure databases

ProteinModelPortaliP33379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0204.

Proteomic databases

PaxDbiP33379.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00731; CAD00731; CAD00731.
GeneIDi987035.
KEGGilmo:lmo0204.
PATRICi20309415. VBILisMon69206_0209.

Phylogenomic databases

HOGENOMiHOG000033794.
KOiK16644.
OMAiSLTYPDK.

Family and domain databases

InterProiIPR007752. Virulence_actor_ActA.
[Graphical view]
PfamiPF05058. ActA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACTA_LISMO
AccessioniPrimary (citable) accession number: P33379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.