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P33379

- ACTA_LISMO

UniProt

P33379 - ACTA_LISMO

Protein

Actin assembly-inducing protein

Gene

actA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Virulence factor required for host cell microfilament interaction. It induces actin assembly around the bacteria to allow it to move within the cytoplasm. It is involved in the actin polymerization process. It seems to act as a nucleator that induces the reorganization of the actin cytoskeleton.

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Biological processi

    Virulence

    Enzyme and pathway databases

    BioCyciLMON169963:LMO0204-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin assembly-inducing protein
    Gene namesi
    Name:actA
    Synonyms:prtB
    Ordered Locus Names:lmo0204
    OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
    Taxonomic identifieri169963 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000817: Chromosome

    Organism-specific databases

    GenoListiLMO0204.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29291 PublicationAdd
    BLAST
    Chaini30 – 639610Actin assembly-inducing proteinPRO_0000020625Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi169963.lmo0204.

    Structurei

    3D structure databases

    ProteinModelPortaliP33379.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei613 – 63321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati264 – 298351Add
    BLAST
    Repeati299 – 333352Add
    BLAST
    Repeati334 – 378453; approximateAdd
    BLAST
    Repeati379 – 417394; approximateAdd
    BLAST
    Repeati418 – 42255; truncated

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni264 – 333705 X approximate tandem repeats, Pro-richAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi360 – 3623Cell attachment siteSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi266 – 33974Pro-richAdd
    BLAST

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000033794.
    KOiK16644.
    OMAiSFEFPPP.
    OrthoDBiEOG6VMTG2.

    Family and domain databases

    InterProiIPR007752. Virulence_actor_ActA.
    [Graphical view]
    PfamiPF05058. ActA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33379-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLNRFMRAM MVVFITANCI TINPDIIFAA TDSEDSSLNT DEWEEEKTEE    50
    QPSEVNTGPR YETAREVSSR DIKELEKSNK VRNTNKADLI AMLKEKAEKG 100
    PNINNNNSEQ TENAAINEEA SGADRPAIQV ERRHPGLPSD SAAEIKKRRK 150
    AIASSDSELE SLTYPDKPTK VNKKKVAKES VADASESDLD SSMQSADESS 200
    PQPLKANQQP FFPKVFKKIK DAGKWVRDKI DENPEVKKAI VDKSAGLIDQ 250
    LLTKKKSEEV NASDFPPPPT DEELRLALPE TPMLLGFNAP ATSEPSSFEF 300
    PPPPTDEELR LALPETPMLL GFNAPATSEP SSFEFPPPPT EDELEIIRET 350
    ASSLDSSFTR GDLASLRNAI NRHSQNFSDF PPIPTEEELN GRGGRPTSEE 400
    FSSLNSGDFT DDENSETTEE EIDRLADLRD RGTGKHSRNA GFLPLNPFAS 450
    SPVPSLSPKV SKISAPALIS DITKKTPFKN PSQPLNVFNK KTTTKTVTKK 500
    PTPVKTAPKL AELPATKPQE TVLRENKTPF IEKQAETNKQ SINMPSLPVI 550
    QKEATESDKE EMKPQTEEKM VEESESANNA NGKNRSAGIE EGKLIAKSAE 600
    DEKAKEEPGN HTTLILAMLA IGVFSLGAFI KIIQLRKNN 639
    Length:639
    Mass (Da):70,349
    Last modified:February 1, 1994 - v1
    Checksum:i5A06CF78BC5F3C91
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti465 – 4662AP → DR in CAA42407. (PubMed:1582425)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82881 Genomic DNA. Translation: AAA25269.1.
    X59723 Genomic DNA. Translation: CAA42407.1.
    AL591974 Genomic DNA. Translation: CAD00731.1.
    PIRiAE1100.
    S20887.
    RefSeqiNP_463735.1. NC_003210.1.
    WP_010989374.1. NC_003210.1.

    Genome annotation databases

    EnsemblBacteriaiCAD00731; CAD00731; CAD00731.
    GeneIDi987035.
    KEGGilmo:lmo0204.
    PATRICi20309415. VBILisMon69206_0209.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82881 Genomic DNA. Translation: AAA25269.1 .
    X59723 Genomic DNA. Translation: CAA42407.1 .
    AL591974 Genomic DNA. Translation: CAD00731.1 .
    PIRi AE1100.
    S20887.
    RefSeqi NP_463735.1. NC_003210.1.
    WP_010989374.1. NC_003210.1.

    3D structure databases

    ProteinModelPortali P33379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 169963.lmo0204.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD00731 ; CAD00731 ; CAD00731 .
    GeneIDi 987035.
    KEGGi lmo:lmo0204.
    PATRICi 20309415. VBILisMon69206_0209.

    Organism-specific databases

    GenoListi LMO0204.

    Phylogenomic databases

    HOGENOMi HOG000033794.
    KOi K16644.
    OMAi SFEFPPP.
    OrthoDBi EOG6VMTG2.

    Enzyme and pathway databases

    BioCyci LMON169963:LMO0204-MONOMER.

    Family and domain databases

    InterProi IPR007752. Virulence_actor_ActA.
    [Graphical view ]
    Pfami PF05058. ActA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread."
      Vazquez-Boland J.-A., Kocks C., Dramsi S., Ohayon H., Geoffroy C., Mengaud J., Cossart P.
      Infect. Immun. 60:219-230(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LO28 / Serovar 1/2c.
    2. "L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein."
      Kocks C., Gouin E., Tabouret M., Berche P., Ohayon H., Cossart P.
      Cell 68:521-531(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin."
      Domann E., Wehland J., Rohde M., Pistor S., Hartl M., Goebel W., Leimeister-Waechter M., Wuensher M., Chakraborty T.
      EMBO J. 11:1981-1990(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-59.
      Strain: EGD / Serovar 1/2a.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-679 / EGD-e.
    5. "The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton."
      Pistor S., Chakraborty T., Niebuhr K., Domann E., Wehland J.
      EMBO J. 13:758-763(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiACTA_LISMO
    AccessioniPrimary (citable) accession number: P33379
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    External Data

    Dasty 3