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P33379

- ACTA_LISMO

UniProt

P33379 - ACTA_LISMO

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Protein

Actin assembly-inducing protein

Gene

actA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Virulence factor required for host cell microfilament interaction. It induces actin assembly around the bacteria to allow it to move within the cytoplasm. It is involved in the actin polymerization process. It seems to act as a nucleator that induces the reorganization of the actin cytoskeleton.

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciLMON169963:LMO0204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin assembly-inducing protein
Gene namesi
Name:actA
Synonyms:prtB
Ordered Locus Names:lmo0204
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO0204.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 639610Actin assembly-inducing proteinPRO_0000020625Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0204.

Structurei

3D structure databases

ProteinModelPortaliP33379.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei613 – 63321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati264 – 298351Add
BLAST
Repeati299 – 333352Add
BLAST
Repeati334 – 378453; approximateAdd
BLAST
Repeati379 – 417394; approximateAdd
BLAST
Repeati418 – 42255; truncated

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 333705 X approximate tandem repeats, Pro-richAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi360 – 3623Cell attachment siteSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi266 – 33974Pro-richAdd
BLAST

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000033794.
KOiK16644.
OMAiSFEFPPP.
OrthoDBiEOG6VMTG2.

Family and domain databases

InterProiIPR007752. Virulence_actor_ActA.
[Graphical view]
PfamiPF05058. ActA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33379-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLNRFMRAM MVVFITANCI TINPDIIFAA TDSEDSSLNT DEWEEEKTEE
60 70 80 90 100
QPSEVNTGPR YETAREVSSR DIKELEKSNK VRNTNKADLI AMLKEKAEKG
110 120 130 140 150
PNINNNNSEQ TENAAINEEA SGADRPAIQV ERRHPGLPSD SAAEIKKRRK
160 170 180 190 200
AIASSDSELE SLTYPDKPTK VNKKKVAKES VADASESDLD SSMQSADESS
210 220 230 240 250
PQPLKANQQP FFPKVFKKIK DAGKWVRDKI DENPEVKKAI VDKSAGLIDQ
260 270 280 290 300
LLTKKKSEEV NASDFPPPPT DEELRLALPE TPMLLGFNAP ATSEPSSFEF
310 320 330 340 350
PPPPTDEELR LALPETPMLL GFNAPATSEP SSFEFPPPPT EDELEIIRET
360 370 380 390 400
ASSLDSSFTR GDLASLRNAI NRHSQNFSDF PPIPTEEELN GRGGRPTSEE
410 420 430 440 450
FSSLNSGDFT DDENSETTEE EIDRLADLRD RGTGKHSRNA GFLPLNPFAS
460 470 480 490 500
SPVPSLSPKV SKISAPALIS DITKKTPFKN PSQPLNVFNK KTTTKTVTKK
510 520 530 540 550
PTPVKTAPKL AELPATKPQE TVLRENKTPF IEKQAETNKQ SINMPSLPVI
560 570 580 590 600
QKEATESDKE EMKPQTEEKM VEESESANNA NGKNRSAGIE EGKLIAKSAE
610 620 630
DEKAKEEPGN HTTLILAMLA IGVFSLGAFI KIIQLRKNN
Length:639
Mass (Da):70,349
Last modified:February 1, 1994 - v1
Checksum:i5A06CF78BC5F3C91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti465 – 4662AP → DR in CAA42407. (PubMed:1582425)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82881 Genomic DNA. Translation: AAA25269.1.
X59723 Genomic DNA. Translation: CAA42407.1.
AL591974 Genomic DNA. Translation: CAD00731.1.
PIRiAE1100.
S20887.
RefSeqiNP_463735.1. NC_003210.1.
WP_010989374.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00731; CAD00731; CAD00731.
GeneIDi987035.
KEGGilmo:lmo0204.
PATRICi20309415. VBILisMon69206_0209.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82881 Genomic DNA. Translation: AAA25269.1 .
X59723 Genomic DNA. Translation: CAA42407.1 .
AL591974 Genomic DNA. Translation: CAD00731.1 .
PIRi AE1100.
S20887.
RefSeqi NP_463735.1. NC_003210.1.
WP_010989374.1. NC_003210.1.

3D structure databases

ProteinModelPortali P33379.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo0204.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00731 ; CAD00731 ; CAD00731 .
GeneIDi 987035.
KEGGi lmo:lmo0204.
PATRICi 20309415. VBILisMon69206_0209.

Organism-specific databases

GenoListi LMO0204.

Phylogenomic databases

HOGENOMi HOG000033794.
KOi K16644.
OMAi SFEFPPP.
OrthoDBi EOG6VMTG2.

Enzyme and pathway databases

BioCyci LMON169963:LMO0204-MONOMER.

Family and domain databases

InterProi IPR007752. Virulence_actor_ActA.
[Graphical view ]
Pfami PF05058. ActA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread."
    Vazquez-Boland J.-A., Kocks C., Dramsi S., Ohayon H., Geoffroy C., Mengaud J., Cossart P.
    Infect. Immun. 60:219-230(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. "L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein."
    Kocks C., Gouin E., Tabouret M., Berche P., Ohayon H., Cossart P.
    Cell 68:521-531(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin."
    Domann E., Wehland J., Rohde M., Pistor S., Hartl M., Goebel W., Leimeister-Waechter M., Wuensher M., Chakraborty T.
    EMBO J. 11:1981-1990(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-59.
    Strain: EGD / Serovar 1/2a.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  5. "The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton."
    Pistor S., Chakraborty T., Niebuhr K., Domann E., Wehland J.
    EMBO J. 13:758-763(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiACTA_LISMO
AccessioniPrimary (citable) accession number: P33379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

External Data

Dasty 3