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Protein

Phospholipase C

Gene

plcB

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Important role in the infectious process. May contribute to efficient lysis of the two-membrane vacuoles that surround the bacteria after direct cell-to-cell spread.

Catalytic activityi

A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine.

Cofactori

Zn2+PROSITE-ProRule annotationNote: Binds 3 Zn2+ ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Zinc 3PROSITE-ProRule annotation
Metal bindingi65 – 651Zinc 3PROSITE-ProRule annotation
Metal bindingi106 – 1061Zinc 1PROSITE-ProRule annotation
Metal bindingi120 – 1201Zinc 1PROSITE-ProRule annotation
Metal bindingi169 – 1691Zinc 1PROSITE-ProRule annotation
Metal bindingi173 – 1731Zinc 1PROSITE-ProRule annotation
Metal bindingi173 – 1731Zinc 3PROSITE-ProRule annotation
Metal bindingi179 – 1791Zinc 2PROSITE-ProRule annotation
Metal bindingi193 – 1931Zinc 2PROSITE-ProRule annotation
Metal bindingi197 – 1971Zinc 2PROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciLMON169963:LMO0205-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase C (EC:3.1.4.3)
Short name:
PLC
Alternative name(s):
Lecithinase
Phosphatidylcholine cholinephosphohydrolase
Gene namesi
Name:plcB
Synonyms:prtC
Ordered Locus Names:lmo0205
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 5126By similarityPRO_0000023937Add
BLAST
Chaini52 – 289238Phospholipase CPRO_0000023938Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP33378.

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0205.

Structurei

3D structure databases

ProteinModelPortaliP33378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 289238Zn-dependent PLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bacterial zinc-metallophospholipase C family.PROSITE-ProRule annotation
Contains 1 Zn-dependent PLC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107HBJ. Bacteria.
ENOG41100NF. LUCA.
KOiK01114.
OMAiCAYENYV.
OrthoDBiEOG6PS5WX.

Family and domain databases

Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR001531. PLipaseC_domain.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFKKVVLGM CLIASVLVFP VTIKANACCD EYLQTPAAPH DIDSKLPHKL
60 70 80 90 100
SWSADNPTNT DVNTHYWLFK QAEKILAKDV NHMRANLMNE LKKFDKQIAQ
110 120 130 140 150
GIYDADHKNP YYDTSTFLSH FYNPDRDNTY LPGFANAKIT GAKYFNQSVT
160 170 180 190 200
DYREGKFDTA FYKLGLAIHY YTDISQPMHA NNFTAISYPP GYHCAYENYV
210 220 230 240 250
DTIKHNYQAT EDMVAKRFCS DDVKDWLYEN AKRAKADYPK IVNAKTKKSY
260 270 280
LVGNSEWKKD TVEPTGARLR DSQQTLAGFL EFWSKKTNE
Length:289
Mass (Da):33,278
Last modified:February 1, 1994 - v1
Checksum:i0F35A2A3EDA6E372
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51K → N in CAA42408 (PubMed:1582425).Curated
Sequence conflicti13 – 131I → T in CAA42408 (PubMed:1582425).Curated
Sequence conflicti222 – 2221D → G in CAA42408 (PubMed:1582425).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82881 Genomic DNA. Translation: AAA25270.1.
X59723 Genomic DNA. Translation: CAA42408.1.
AL591974 Genomic DNA. Translation: CAD00732.1.
PIRiAF1100.
C43868.
S20888.
RefSeqiNP_463736.1. NC_003210.1.
WP_010989375.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00732; CAD00732; CAD00732.
GeneIDi987036.
KEGGilmo:lmo0205.
PATRICi20309417. VBILisMon69206_0210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82881 Genomic DNA. Translation: AAA25270.1.
X59723 Genomic DNA. Translation: CAA42408.1.
AL591974 Genomic DNA. Translation: CAD00732.1.
PIRiAF1100.
C43868.
S20888.
RefSeqiNP_463736.1. NC_003210.1.
WP_010989375.1. NC_003210.1.

3D structure databases

ProteinModelPortaliP33378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0205.

Proteomic databases

PaxDbiP33378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00732; CAD00732; CAD00732.
GeneIDi987036.
KEGGilmo:lmo0205.
PATRICi20309417. VBILisMon69206_0210.

Phylogenomic databases

eggNOGiENOG4107HBJ. Bacteria.
ENOG41100NF. LUCA.
KOiK01114.
OMAiCAYENYV.
OrthoDBiEOG6PS5WX.

Enzyme and pathway databases

BioCyciLMON169963:LMO0205-MONOMER.

Family and domain databases

Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR001531. PLipaseC_domain.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread."
    Vazquez-Boland J.-A., Kocks C., Dramsi S., Ohayon H., Geoffroy C., Mengaud J., Cossart P.
    Infect. Immun. 60:219-230(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. "A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin."
    Domann E., Wehland J., Rohde M., Pistor S., Hartl M., Goebel W., Leimeister-Waechter M., Wuensher M., Chakraborty T.
    EMBO J. 11:1981-1990(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD / Serovar 1/2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  4. "Purification and characterization of an extracellular 29-kilodalton phospholipase C from Listeria monocytogenes."
    Geoffroy C., Raveneau J., Beretti J.-L., Lecroisey A., Vazquez-Boland J.-A., Alouf J.E., Berche P.
    Infect. Immun. 59:2382-2388(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 144-152.

Entry informationi

Entry nameiPHLC_LISMO
AccessioniPrimary (citable) accession number: P33378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.