P33377 (PHL3_BACCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelinase C
Short name=SMase
EC=3.1.4.12

Alternative name(s):
Cereolysin B
SMPLC
Sphingomyelin phosphodiesterase

Gene names

Name:

cerB

Organism

Bacillus cereus

Taxonomic identifier

1396 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium.
Enzyme regulation	Activated by cobalt and manganese ions.
Subcellular location	Secreted.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 333	307	Sphingomyelinase C		PRO_0000019901
Amino acid modifications
	Disulfide bond	150 ↔ 186		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33377 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 05F8AEA5ECC4A3B8	FASTA	333	36,821
	10 20 30 40 50 60 MKGKLLKGVL SLGVGLGALY SGTSAQAEAS TNQNDTLKVM THNVYMLSTN LYPNWGQTER 70 80 90 100 110 120 ADLFGAADYI KNQDVVILNE VFDNSASDRL LGNLKKEYPN QTAVLGRSSG SEWDKTLGNY 130 140 150 160 170 180 SSSTPEDGGV AIVSKWPIAE KIQYVFAKGC GPDNLSNKGF VYTKIKKNDR FVHVIGTHLQ 190 200 210 220 230 240 AEDSMCGKTS PASVRTNQLK EIQDFIKNKN IPNNEYVLIG GDMNVNKINA ENNNDSEYAS 250 260 270 280 290 300 MFKTLNASVP SYTGHTATWD ATTNSIAKYN FPDSLAEYLD YIIASKDHAN PSYIENKVLQ 310 320 330 PKSPQWTVTS WFKNIRIMIT LIIIQVEATI SMK

References

[1]

"A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage." Gilmore M.S., Cruz-Rodz A.L., Leimeister-Waechter M., Kreft J., Goebel W. J. Bacteriol. 171:744-753(1989) [PubMed: 2536680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GP-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24149 Genomic DNA. Translation: AAA91820.1.
3D structure databases
ProteinModelPortal	P33377.
SMR	P33377. Positions 34-332.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR017766. Sphingomyelinase/PLipase_C. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
TIGRFAMs	TIGR03395. Sphingomy. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHL3_BACCE

Accession

Primary (citable) accession number: P33377

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 19, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program