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Protein

Phospholipase C

Gene

cerA

Organism
Bacillus cereus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).

Catalytic activityi

A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine.

Cofactori

Zn2+Note: Binds 3 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Zinc 31
Metal bindingi52Zinc 31
Metal bindingi93Zinc 11
Metal bindingi107Zinc 11
Metal bindingi156Zinc 11
Metal bindingi160Zinc 11
Metal bindingi160Zinc 31
Metal bindingi166Zinc 21
Metal bindingi180Zinc 21
Metal bindingi184Zinc 21

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytolysis, Hemolysis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase C (EC:3.1.4.3)
Short name:
PLC
Alternative name(s):
Cereolysin A
Phosphatidylcholine cholinephosphohydrolase
Gene namesi
Name:cerA
OrganismiBacillus cereus
Taxonomic identifieri1396 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002392925 – 38By similarityAdd BLAST14
ChainiPRO_000002393039 – 283Phospholipase CAdd BLAST245

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi226900.BC0670.

Structurei

3D structure databases

ProteinModelPortaliP33376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 283Zn-dependent PLCPROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Belongs to the bacterial zinc-metallophospholipase C family.PROSITE-ProRule annotation
Contains 1 Zn-dependent PLC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd11009. Zn_dep_PLPC. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR029002. PLPC/GPLD1.
IPR001531. Zn_PLipaseC.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKVLALGA AITLVAPLQS VAFAHENDGG QRFGVIPRWS AEDKHKEGVN
60 70 80 90 100
SHLWIVNRAI DIMSRNTTLV KQDRVALLNE WRTELENGIY AADYENPYYD
110 120 130 140 150
NSTFASHFYD PDNGKTYIPY AKQAKETGAK YFKLAGESYK NKDMKQAFFY
160 170 180 190 200
LGLSLHYLGD VNQPMHAANF TNLSYPQGFH SKYENFVDTI KDNYKVTDGN
210 220 230 240 250
GYWNWKGTNP EDWIHGAAVV AKQDYAGIVN DNTKDWFVRA AVSQEYADKW
260 270 280
RAEVTPMTGK RLMDAQRVTA GYIQLWFDTY GNR
Length:283
Mass (Da):32,373
Last modified:February 1, 1994 - v1
Checksum:i24D2E753402A6A44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24149 Genomic DNA. Translation: AAA91819.1.
M35411 Genomic DNA. Translation: AAA22524.1.
PIRiS18978. PS0197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24149 Genomic DNA. Translation: AAA91819.1.
M35411 Genomic DNA. Translation: AAA22524.1.
PIRiS18978. PS0197.

3D structure databases

ProteinModelPortaliP33376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC0670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd11009. Zn_dep_PLPC. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR029002. PLPC/GPLD1.
IPR001531. Zn_PLipaseC.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHLD_BACCE
AccessioniPrimary (citable) accession number: P33376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.