Reviewed, UniProtKB/Swiss-Prot P33375 (MBHS_ALCHY)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:

hupS

Organism

Alcaligenes hydrogenophilus

Taxonomic identifier

516 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 43	43	Tat-type signal Potential
	Chain	44 – 363	320	Uptake hydrogenase small subunit		PRO_0000013422
Sites
	Metal binding	60	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	192	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	230	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	233	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	258	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	264	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	273	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	292	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	295	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33375-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1327F5062D81A61B	FASTA	363	39,696
	10 20 30 40 50 60 MIETFYEVMR RQGISRRSFL KYCSLTATSL GLSPVFVPKI VHAMETKPRT PVLWLHGLEC 70 80 90 100 110 120 TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILGEVMTKY KGNYILAVEG 130 140 150 160 170 180 NPPLNQDGMS CIIGGKPFID QLRHVAKDAK AIISWGSCAS WGCVQAAKAN PTQATPIHKV 190 200 210 220 230 240 ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RFPELDRQGR PKMFYSQRIH DKCYRRPHFD 250 260 270 280 290 300 AGQFVESWDD ESARKGYCLY KVGCKGPTTY NACSTTRWNG GTSFPIQSGH GCIGCSEDGF 310 320 330 340 350 360 WDKGSFYSRL TNIHQFGIEA NADSVGVTAV GVVGAATAAH AAVSAIKRAR HKDAAQDTAA TQK

References

[1]	"Nucleotide sequences of membrane-bound hydrogenase gene in Alcaligenes hydrogenophilus." Yagi K., Seto T., Terakado M., Umeda F., Doi T., Imanishi T., Miura Y. Chem. Pharm. Bull. 40:3292-3296(1992) [PubMed: 1294332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	S56898 Genomic DNA. Translation: AAB25779.1.
PIR	JH0775.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 289463.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_ALCHY

Accession

Primary (citable) accession number: P33375

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)