Reviewed, UniProtKB/Swiss-Prot P33374 (MBHL_ALCHY)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit

Gene names

Name:

hupL

Organism

Alcaligenes hydrogenophilus

Taxonomic identifier

516 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Uptake hydrogenase large subunit		PRO_0000199707
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	600	1	Nickel Potential
	Metal binding	603	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P33374-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 906AC4414285D4A3	FASTA	621	68,925
	10 20 30 40 50 60 MATYETQGFK LNDSGRRIIV DPVTRIEGHM RCEVNLDANN VIRNAVSTGT MWRGLEVILK 70 80 90 100 110 120 RADPADAWAF VERICRVCTG CHALASVRAV EDALGIKIPK NAHLIREMMA KTLQVHDHVV 130 140 150 160 170 180 HFYHLHALDW VDVVSALNAD PKRTSALQQT VSPAHPLSSP GYFRDVQIRL KKFVESGQLG 190 200 210 220 230 240 PFMNGYWGNP AYKLPPEANL MAVTHYLEAL DLQKEWVKIH TIFGGKNPHP NYLVGGMPCV 250 260 270 280 290 300 DSNLDGSGAA GAPLNMERLN FVRARIEEAI EFVKNVYLPD VLAIGTIYKD AGWLYGGGLS 310 320 330 340 350 360 ALNVMDYGTY PRVNYDPTTD QLPGGAILNG NWDEIFPVDP RDPEQVQEFV AHSWYKYADE 370 380 390 400 410 420 TKGLHPWDGV TEPNFVLGPK AVGTPTDIKQ LDEDAKYSWI KVAALAGHAM EVGPLVALHP 430 440 450 460 470 480 RIRARAEDPK SYRAHYLREQ VENSARAINT GIPQALGLKQ TDYTVKQLLP TTIGRTLARA 490 500 510 520 530 540 LEAQYCGNMM LDDWHEMMAN IKAGDLTTAN VDKWEPSAWP KEAKGVGHVA APRGACGHWI 550 560 570 580 590 600 RIKDGKIENY QCVVPTTWNG SPRDSKGQIG AFEASLMNTP MAKPEEPVEI LRTVHSFDPC 610 620 LACSTHVIRP DGQERVVVKV R

References

[1]	"Nucleotide sequences of membrane-bound hydrogenase gene in Alcaligenes hydrogenophilus." Yagi K., Seto T., Terakado M., Umeda F., Doi T., Imanishi T., Miura Y. Chem. Pharm. Bull. 40:3292-3296(1992) [PubMed: 1294332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	S56898 Genomic DNA. Translation: AAB25780.1. Sequence problems.
PIR	JH0776.
3D structure databases
HSSP	HSSP built from PDB template 1UBL based on UniProtKB P21852.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 289463.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_ALCHY

Accession

Primary (citable) accession number: P33374

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)