Reviewed, UniProtKB/Swiss-Prot P33371 (DUSC_ECOLI)

Names and origin

Protein names

Recommended name:
    tRNA-dihydrouridine synthase C
    EC=1.-.-.-

Gene names

Name:	dusC
Synonyms:	yohI
Ordered Locus Names:	b2140, JW2128

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Ref.4
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family. DusC subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-562765,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	tRNA-dihydrouridine synthase C		PRO_0000162109

Sequences

	Sequence		Length	Mass (Da)
	P33371-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 83B33E5034E53565	FASTA	315	35,199
	10 20 30 40 50 60 MRVLLAPMEG VLDSLVRELL TEVNDYDLCI TEFVRVVDQL LPVKVFHRIC PELQNASRTP 70 80 90 100 110 120 SGTLVRVQLL GQFPQWLAEN AARAVELGSW GVDLNCGCPS KTVNGSGGGA TLLKDPELIY 130 140 150 160 170 180 QGAKAMREAV PAHLPVSVKV RLGWDSGEKK FEIADAVQQA GATELVVHGR TKEQGYRAEH 190 200 210 220 230 240 IDWQAIGDIR QRLNIPVIAN GEIWDWQSAQ QCMAISGCDA VMIGRGALNI PNLSRVVKYN 250 260 270 280 290 300 EPRMPWPEVV ALLQKYTRLE KQGDTGLYHV ARIKQWLSYL RKEYDEATEL FQHVRVLNNS 310 PDIARAIQAI DIEKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of the tRNA-dihydrouridine synthase family." Bishop A.C., Xu J., Johnson R.C., Schimmel P., de Crecy-Lagard V. J. Biol. Chem. 277:25090-25095(2002) [PubMed: 11983710] [Abstract] Cited for: FUNCTION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00007 Genomic DNA. Translation: AAA60503.1. U00096 Genomic DNA. Translation: AAC75201.1. AP009048 Genomic DNA. Translation: BAE76617.1.
PIR	C64982.
RefSeq	AP_002737.1. NP_416645.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12808N.
IntAct	P33371. 24 interactions.
Genome annotation databases
GeneID	945458.
GenomeReviews	Gene locus JW2128 in contig AP009048_GR. Gene locus b2140 in contig U00096_GR.
KEGG	ecj:JW2128. eco:b2140.
Organism-specific databases
EchoBASE	EB1957.
EcoGene	EG12022. dusC.
CMR	Search...
Phylogenomic databases
HOGENOM	P33371.
OMA	P33371. VGGIDWC.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12022-MON.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11082. Du_synth. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSC_ECOLI

Accession

Primary (citable) accession number: P33371
Secondary accession number(s): Q2MAT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)