P33368 (YOHF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized oxidoreductase YohF
EC=1.-.-.-

Gene names

Name:	yohF
Synonyms:	yohE
Ordered Locus Names:	b2137, JW2125

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence caution	The sequence AAA60499.1 differs from that shown. Reason: Frameshift at position 185. Produces two separate ORFs.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Uncharacterized oxidoreductase YohF		PRO_0000054840
Regions
	Nucleotide binding	6 – 30	25	NAD or NADP By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33368 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B030F9EAD7E8F42A	FASTA	253	26,951
	10 20 30 40 50 60 MAQVAIITAS DSGIGKECAL LLAQQGFDIG ITWHSDEEGA KDTAREVVSH GVRAEIVQLD 70 80 90 100 110 120 LGNLPEGALA LEKLIQRLGR IDVLVNNAGA MTKAPFLDMA FDEWRKIFTV DVDGAFLCSQ 130 140 150 160 170 180 IAARQMVKQG QGGRIINITS VHEHTPLPDA SAYTAAKHAL GGLTKAMALE LVRHKILVNA 190 200 210 220 230 240 VAPGAIATPM NGMDDSDVKP DAEPSIPLRR FGATHEIASL VVWLCSEGAN YTTGQSLIVD 250 GGFMLANPQF NPE

References

[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00007 Genomic DNA. Translation: AAA60500.1. Frameshift. U00007 Genomic DNA. Translation: AAA60499.1. Frameshift. U00096 Genomic DNA. Translation: AAC75198.1. AP009048 Genomic DNA. Translation: BAE76614.1.
PIR	H64981.
RefSeq	NP_416641.1. NC_000913.2. YP_490376.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P33368.
SMR	P33368. Positions 2-242.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12806N.
IntAct	P33368. 6 interactions.
STRING	511145.b2137.
Proteomic databases
PaxDb	P33368.
PRIDE	P33368.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75198; AAC75198; b2137. BAE76614; BAE76614; BAE76614.
GeneID	12931460. 949126.
KEGG	ecj:Y75_p2099. eco:b2137.
PATRIC	32119617. VBIEscCol129921_2219.
Organism-specific databases
EchoBASE	EB1955.
EcoGene	EG12019. yohF.
Phylogenomic databases
eggNOG	COG1028.
KO	K00540.
OMA	FIIDGGF.
ProtClustDB	PRK12743.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12019-MONOMER. ECOL316407:JW2125-MONOMER.
Gene expression databases
Genevestigator	P33368.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YOHF_ECOLI

Accession

Primary (citable) accession number: P33368
Secondary accession number(s): P33367, P76437, Q2MAU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program