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P33368 (YOHF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized oxidoreductase YohF

EC=1.-.-.-
Gene names
Name:yohF
Synonyms:yohE
Ordered Locus Names:b2137, JW2125
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence caution

The sequence AAA60499.1 differs from that shown. Reason: Frameshift at position 185. Produces two separate ORFs.

Ontologies

Keywords
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 253253Uncharacterized oxidoreductase YohF
PRO_0000054840

Regions

Nucleotide binding6 – 3025NAD or NADP By similarity

Sites

Active site1531Proton acceptor By similarity
Binding site1401Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P33368 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: B030F9EAD7E8F42A

FASTA25326,951
        10         20         30         40         50         60 
MAQVAIITAS DSGIGKECAL LLAQQGFDIG ITWHSDEEGA KDTAREVVSH GVRAEIVQLD 

        70         80         90        100        110        120 
LGNLPEGALA LEKLIQRLGR IDVLVNNAGA MTKAPFLDMA FDEWRKIFTV DVDGAFLCSQ 

       130        140        150        160        170        180 
IAARQMVKQG QGGRIINITS VHEHTPLPDA SAYTAAKHAL GGLTKAMALE LVRHKILVNA 

       190        200        210        220        230        240 
VAPGAIATPM NGMDDSDVKP DAEPSIPLRR FGATHEIASL VVWLCSEGAN YTTGQSLIVD 

       250 
GGFMLANPQF NPE 

« Hide

References

[1]"Automated multiplex sequencing of the E.coli genome."
Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / BHB2600.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00007 Genomic DNA. Translation: AAA60500.1. Frameshift.
U00007 Genomic DNA. Translation: AAA60499.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75198.1.
AP009048 Genomic DNA. Translation: BAE76614.1.
PIRH64981.
RefSeqNP_416641.1. NC_000913.3.
YP_490376.1. NC_007779.1.

3D structure databases

ProteinModelPortalP33368.
SMRP33368. Positions 2-242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12806N.
IntActP33368. 6 interactions.
STRING511145.b2137.

Proteomic databases

PaxDbP33368.
PRIDEP33368.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75198; AAC75198; b2137.
BAE76614; BAE76614; BAE76614.
GeneID12931460.
949126.
KEGGecj:Y75_p2099.
eco:b2137.
PATRIC32119617. VBIEscCol129921_2219.

Organism-specific databases

EchoBASEEB1955.
EcoGeneEG12019. yohF.

Phylogenomic databases

eggNOGCOG1028.
KOK00540.
OMAYTTGQSF.
OrthoDBEOG6N3CR8.
PhylomeDBP33368.
ProtClustDBPRK12743.

Enzyme and pathway databases

BioCycEcoCyc:EG12019-MONOMER.
ECOL316407:JW2125-MONOMER.

Gene expression databases

GenevestigatorP33368.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP33368.

Entry information

Entry nameYOHF_ECOLI
AccessionPrimary (citable) accession number: P33368
Secondary accession number(s): P33367, P76437, Q2MAU2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene