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Protein

Uncharacterized oxidoreductase YohF

Gene

yohF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401SubstrateBy similarity
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 3025NAD or NADPBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:EG12019-MONOMER.
ECOL316407:JW2125-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized oxidoreductase YohF (EC:1.-.-.-)
Gene namesi
Name:yohF
Synonyms:yohE
Ordered Locus Names:b2137, JW2125
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12019. yohF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Uncharacterized oxidoreductase YohFPRO_0000054840Add
BLAST

Proteomic databases

PaxDbiP33368.
PRIDEiP33368.

Expressioni

Gene expression databases

GenevestigatoriP33368.

Interactioni

Protein-protein interaction databases

DIPiDIP-12806N.
IntActiP33368. 6 interactions.
STRINGi511145.b2137.

Structurei

3D structure databases

ProteinModelPortaliP33368.
SMRiP33368. Positions 2-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP33368.
KOiK00540.
OMAiMNGMDDG.
OrthoDBiEOG6N3CR8.
PhylomeDBiP33368.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVAIITAS DSGIGKECAL LLAQQGFDIG ITWHSDEEGA KDTAREVVSH
60 70 80 90 100
GVRAEIVQLD LGNLPEGALA LEKLIQRLGR IDVLVNNAGA MTKAPFLDMA
110 120 130 140 150
FDEWRKIFTV DVDGAFLCSQ IAARQMVKQG QGGRIINITS VHEHTPLPDA
160 170 180 190 200
SAYTAAKHAL GGLTKAMALE LVRHKILVNA VAPGAIATPM NGMDDSDVKP
210 220 230 240 250
DAEPSIPLRR FGATHEIASL VVWLCSEGAN YTTGQSLIVD GGFMLANPQF

NPE
Length:253
Mass (Da):26,951
Last modified:November 1, 1997 - v2
Checksum:iB030F9EAD7E8F42A
GO

Sequence cautioni

The sequence AAA60499.1 differs from that shown. Reason: Frameshift at position 185. Produces two separate ORFs.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60500.1. Frameshift.
U00007 Genomic DNA. Translation: AAA60499.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75198.1.
AP009048 Genomic DNA. Translation: BAE76614.1.
PIRiH64981.
RefSeqiNP_416641.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75198; AAC75198; b2137.
BAE76614; BAE76614; BAE76614.
GeneIDi949126.
KEGGiecj:Y75_p2099.
eco:b2137.
PATRICi32119617. VBIEscCol129921_2219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60500.1. Frameshift.
U00007 Genomic DNA. Translation: AAA60499.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75198.1.
AP009048 Genomic DNA. Translation: BAE76614.1.
PIRiH64981.
RefSeqiNP_416641.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP33368.
SMRiP33368. Positions 2-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12806N.
IntActiP33368. 6 interactions.
STRINGi511145.b2137.

Proteomic databases

PaxDbiP33368.
PRIDEiP33368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75198; AAC75198; b2137.
BAE76614; BAE76614; BAE76614.
GeneIDi949126.
KEGGiecj:Y75_p2099.
eco:b2137.
PATRICi32119617. VBIEscCol129921_2219.

Organism-specific databases

EchoBASEiEB1955.
EcoGeneiEG12019. yohF.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP33368.
KOiK00540.
OMAiMNGMDDG.
OrthoDBiEOG6N3CR8.
PhylomeDBiP33368.

Enzyme and pathway databases

BioCyciEcoCyc:EG12019-MONOMER.
ECOL316407:JW2125-MONOMER.

Miscellaneous databases

PROiP33368.

Gene expression databases

GenevestigatoriP33368.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYOHF_ECOLI
AccessioniPrimary (citable) accession number: P33368
Secondary accession number(s): P33367, P76437, Q2MAU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: May 27, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.