P33363 (BGLX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic beta-glucosidase
EC=3.2.1.21

Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase

Gene names

Name:	bglX
Synonyms:	yohA
Ordered Locus Names:	b2132, JW2120

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.
Sequence caution	The sequence AAA60495.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from direct assay. Source: EcoliWiki
Molecular function	beta-glucosidase activity Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nrdA	P00452	1	EBI-1114600,EBI-370018

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 765	745	Periplasmic beta-glucosidase		PRO_0000011781
Sites
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33363 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 0E89B0AB42B8F8F3	FASTA	765	83,460
	10 20 30 40 50 60 MKWLCSVGIA VSLALQPALA DDLFGNHPLT PEARDAFVTE LLKKMTVDEK IGQLRLISVG 70 80 90 100 110 120 PDNPKEAIRE MIKDGQVGAI FNTVTRQDIR AMQDQVMELS RLKIPLFFAY DVLHGQRTVF 130 140 150 160 170 180 PISLGLASSF NLDAVKTVGR VSAYEAADDG LNMTWAPMVD VSRDPRWGRA SEGFGEDTYL 190 200 210 220 230 240 TSTMGKTMVE AMQGKSPADR YSVMTSVKHF AAYGAVEGGK EYNTVDMSPQ RLFNDYMPPY 250 260 270 280 290 300 KAGLDAGSGA VMVALNSLNG TPATSDSWLL KDVLRDQWGF KGITVSDHGA IKELIKHGTA 310 320 330 340 350 360 ADPEDAVRVA LKSGINMSMS DEYYSKYLPG LIKSGKVTMA ELDDAARHVL NVKYDMGLFN 370 380 390 400 410 420 DPYSHLGPKE SDPVDTNAES RLHRKEAREV ARESLVLLKN RLETLPLKKS ATIAVVGPLA 430 440 450 460 470 480 DSKRDVMGSW SAAGVADQSV TVLTGIKNAV GENGKVLYAK GANVTSDKGI IDFLNQYEEA 490 500 510 520 530 540 VKVDPRSPQE MIDEAVQTAK QSDVVVAVVG EAQGMAHEAS SRTDITIPQS QRDLIAALKA 550 560 570 580 590 600 TGKPLVLVLM NGRPLALVKE DQQADAILET WFAGTEGGNA IADVLFGDYN PSGKLPMSFP 610 620 630 640 650 660 RSVGQIPVYY SHLNTGRPYN ADKPNKYTSR YFDEANGALY PFGYGLSYTT FTVSDVKLSA 670 680 690 700 710 720 PTMKRDGKVT ASVQVTNTGK REGATVVQMY LQDVTASMSR PVKQLKGFEK ITLKPGETQT 730 740 750 760 VSFPIDIEAL KFWNQQMKYD AEPGKFNVFI GTDSARVKKG EFELL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yang M., Luoh S., Goddard A., Reilly D., Henzel W., Bass S. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15049 Genomic DNA. Translation: AAB38487.1. U00007 Genomic DNA. Translation: AAA60495.1. Different initiation. U00096 Genomic DNA. Translation: AAC75193.1. AP009048 Genomic DNA. Translation: BAE76609.1.
PIR	C64981.
RefSeq	NP_416636.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P33363.
SMR	P33363. Positions 22-764.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9218N.
IntAct	P33363. 16 interactions.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Proteomic databases
PRIDE	P33363.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004477; EBESCP00000004477; EBESCG00000003653. EBESCT00000016990; EBESCP00000016281; EBESCG00000016049.
GeneID	946682.
GenomeReviews	Gene locus JW2120 in contig AP009048_GR. Gene locus b2132 in contig U00096_GR.
KEGG	ecj:JW2120. eco:b2132.
PATRIC	32119603. VBIEscCol129921_2212.
Organism-specific databases
EchoBASE	EB1951.
EcoGene	EG12013. bglX.
Phylogenomic databases
eggNOG	COG1472.
GeneTree	EBGT00050000011144.
HOGENOM	HBG749096.
OMA	RTNITIP.
PhylomeDB	P33363.
ProtClustDB	PRK15098.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12013-MONOMER. MetaCyc:EG12013-MONOMER.
Gene expression databases
Genevestigator	P33363.
Family and domain databases
InterPro	IPR019800. Glyco_hydro_3_AS. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.40.50.1700. G3DSA:3.40.50.1700. 1 hit. G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
KO	K05349.
Pfam	PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SUPFAM	SSF52279. Glyco_hydro_3_C. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLX_ECOLI

Accession

Primary (citable) accession number: P33363
Secondary accession number(s): Q2MAU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program