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Protein

Glycine betaine-binding protein YehZ

Gene

yehZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Binds glycine betaine with low affinity.1 Publication

GO - Molecular functioni

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • cellular hyperosmotic response Source: EcoCyc
  • glycine betaine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEHZ-MONOMER.
ECOL316407:JW2119-MONOMER.
RETL1328306-WGS:GSTH-497-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.15. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine-binding protein YehZCurated
Gene namesi
Name:yehZ
Synonyms:osmF
Ordered Locus Names:b2131, JW2119
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12012. osmF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 305282Glycine betaine-binding protein YehZPRO_0000013871Add
BLAST

Proteomic databases

EPDiP33362.
PaxDbiP33362.
PRIDEiP33362.

Expressioni

Inductioni

Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ).Curated

Protein-protein interaction databases

BioGridi4260450. 93 interactions.
IntActiP33362. 3 interactions.
STRINGi511145.b2131.

Structurei

Secondary structure

1
305
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 348Combined sources
Helixi35 – 5016Combined sources
Beta strandi55 – 639Combined sources
Helixi64 – 718Combined sources
Turni72 – 743Combined sources
Beta strandi77 – 826Combined sources
Helixi83 – 853Combined sources
Helixi86 – 894Combined sources
Helixi96 – 994Combined sources
Helixi101 – 11616Combined sources
Beta strandi118 – 1203Combined sources
Beta strandi129 – 1357Combined sources
Helixi136 – 1416Combined sources
Helixi147 – 1559Combined sources
Beta strandi162 – 1654Combined sources
Helixi166 – 1705Combined sources
Helixi175 – 1828Combined sources
Helixi188 – 1903Combined sources
Beta strandi191 – 1944Combined sources
Helixi199 – 2079Combined sources
Helixi210 – 2123Combined sources
Beta strandi215 – 2195Combined sources
Helixi223 – 2275Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi245 – 2506Combined sources
Helixi251 – 2566Combined sources
Helixi260 – 26910Combined sources
Helixi273 – 28412Combined sources
Helixi290 – 30011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WEPX-ray1.50A/B24-305[»]
ProteinModelPortaliP33362.
SMRiP33362. Positions 27-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OsmX family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107SI7. Bacteria.
COG1732. LUCA.
HOGENOMiHOG000015716.
InParanoidiP33362.
KOiK05845.
OMAiAAFFFEK.
PhylomeDBiP33362.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLKLWAGS LVMLAAVSLP LQAASPVKVG SKIDTEGALL GNIILQVLES
60 70 80 90 100
HGVPTVNKVQ LGTTPVVRGA ITSGELDIYP EYTGNGAFFF KDENDAAWKN
110 120 130 140 150
AQQGYEKVKK LDSEHNKLIW LTPAPANNTW TIAVRQDVAE KNKLTSLADL
160 170 180 190 200
SRYLQEGGTF KLAASAEFIE RADALPAFEK AYGFKLGQDQ LLSLAGGDTA
210 220 230 240 250
VTIKAAAQQT SGVNAAMAYG TDGPVAALGL QTLSDPQGVQ PIYAPAPVVR
260 270 280 290 300
ESVLREYPQM AQWLQPVFAS LDAKTLQQLN ASIAVEGLDA KKVAADYLKQ

KGWTK
Length:305
Mass (Da):32,609
Last modified:February 1, 1994 - v1
Checksum:i7823749E8575B784
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60494.1.
U00096 Genomic DNA. Translation: AAC75192.1.
AP009048 Genomic DNA. Translation: BAE76608.1.
PIRiB64981.
RefSeqiNP_416635.1. NC_000913.3.
WP_001130308.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75192; AAC75192; b2131.
BAE76608; BAE76608; BAE76608.
GeneIDi946681.
KEGGiecj:JW2119.
eco:b2131.
PATRICi32119601. VBIEscCol129921_2211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60494.1.
U00096 Genomic DNA. Translation: AAC75192.1.
AP009048 Genomic DNA. Translation: BAE76608.1.
PIRiB64981.
RefSeqiNP_416635.1. NC_000913.3.
WP_001130308.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WEPX-ray1.50A/B24-305[»]
ProteinModelPortaliP33362.
SMRiP33362. Positions 27-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260450. 93 interactions.
IntActiP33362. 3 interactions.
STRINGi511145.b2131.

Protein family/group databases

TCDBi3.A.1.12.15. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP33362.
PaxDbiP33362.
PRIDEiP33362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75192; AAC75192; b2131.
BAE76608; BAE76608; BAE76608.
GeneIDi946681.
KEGGiecj:JW2119.
eco:b2131.
PATRICi32119601. VBIEscCol129921_2211.

Organism-specific databases

EchoBASEiEB1950.
EcoGeneiEG12012. osmF.

Phylogenomic databases

eggNOGiENOG4107SI7. Bacteria.
COG1732. LUCA.
HOGENOMiHOG000015716.
InParanoidiP33362.
KOiK05845.
OMAiAAFFFEK.
PhylomeDBiP33362.

Enzyme and pathway databases

BioCyciEcoCyc:YEHZ-MONOMER.
ECOL316407:JW2119-MONOMER.
RETL1328306-WGS:GSTH-497-MONOMER.

Miscellaneous databases

PROiP33362.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYEHZ_ECOLI
AccessioniPrimary (citable) accession number: P33362
Secondary accession number(s): Q2MAU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be part of an osmoprotectant uptake system (PubMed:15251200). However, it was shown later that the complex does not mediate osmotic stress protection (PubMed:26325238).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.