P33361 (YEHY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative osmoprotectant uptake system permease protein YehY

Gene names

Name:	yehY
Ordered Locus Names:	b2130, JW2118

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be part of an ABC transporter complex involved in uptake of osmoprotectant molecules. Probably responsible for the translocation of the substrate across the membrane. Ref.4
Subunit structure	The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (OsmF) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Induction	Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase. Ref.4
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 1 ABC transmembrane type-1 domain.
Sequence caution	The sequence AAA60493.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Putative osmoprotectant uptake system permease protein YehY		PRO_0000060256
Regions
	Topological domain	1 – 5	5	Cytoplasmic Potential
	Transmembrane	6 – 26	21	Helical; Potential
	Topological domain	27 – 44	18	Periplasmic Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Topological domain	66 – 69	4	Cytoplasmic Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Topological domain	91 – 106	16	Periplasmic Potential
	Transmembrane	107 – 127	21	Helical; Potential
	Topological domain	128 – 137	10	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 180	22	Periplasmic Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Topological domain	202 – 224	23	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Topological domain	246 – 247	2	Periplasmic Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Topological domain	269 – 322	54	Cytoplasmic Potential
	Transmembrane	323 – 343	21	Helical; Potential
	Topological domain	344 – 345	2	Periplasmic Potential
	Transmembrane	346 – 366	21	Helical; Potential
	Topological domain	367 – 385	19	Cytoplasmic Potential
	Domain	177 – 372	196	ABC transmembrane type-1
Experimental info
	Sequence conflict	346 – 385	40	LLSSA…VKRND → AAKQRH in AAA60493. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33361 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E57055E3A2B141CC	FASTA	385	41,139
	10 20 30 40 50 60 MTYFRINPVL ALLLLLTAIA AALPFISYAP NRLVSGEGRH LWQLWPQTIW MLVGVGCAWL 70 80 90 100 110 120 TACFIPGKKG SICALILAQF VFVLLVWGAG KAATQLAQNG SALARTSLGS GFWLAAALAL 130 140 150 160 170 180 LACSDAIRRI STHPLWRWLL HMQIAIIPLW LLYSGTLNDL SLMKEYANRQ DVFDDALAQH 190 200 210 220 230 240 LTLLFGAVLP ALVIGVPLGI WCYFSTARQG AIFSLLNVIQ TVPSVALFGL LIAPLAALVT 250 260 270 280 290 300 AFPWLGTLGI AGTGMTPALI ALVLYALLPL VRGVVVGLNQ IPRDVLESAR AMGMSGAQRF 310 320 330 340 350 360 LHVQLPLALP VFLRSLRVVM VQTVGMAVIA ALIGAGGFGA LVFQGLLSSA IDLVLLGVIP 370 380 VIVLAVLTDA LFDLLIALLK VKRND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sigma(s)-dependent regulation of yehZYXW, which encodes a putative osmoprotectant ABC transporter of Escherichia coli." Checroun C., Gutierrez C. FEMS Microbiol. Lett. 236:221-226(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00007 Genomic DNA. Translation: AAA60493.1. Different initiation. U00096 Genomic DNA. Translation: AAC75191.1. AP009048 Genomic DNA. Translation: BAE76607.1.
PIR	A64981.
RefSeq	NP_416634.1. NC_000913.2. YP_490369.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P33361.
SMR	P33361. Positions 179-327.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11913N.
IntAct	P33361. 1 interaction.
MINT	MINT-1271646.
STRING	511145.b2130.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75191; AAC75191; b2130. BAE76607; BAE76607; BAE76607.
GeneID	12931455. 946660.
KEGG	ecj:Y75_p2092. eco:b2130.
PATRIC	32119599. VBIEscCol129921_2210.
Organism-specific databases
EchoBASE	EB1949.
EcoGene	EG12011. yehY.
Phylogenomic databases
eggNOG	COG1174.
HOGENOM	HOG000218077.
KO	K05846.
OMA	VVANTVV.
ProtClustDB	CLSK880307.
Enzyme and pathway databases
BioCyc	EcoCyc:YEHY-MONOMER. ECOL316407:JW2118-MONOMER.
Gene expression databases
Genevestigator	P33361.
Family and domain databases
InterPro	IPR000515. BPD_transp. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YEHY_ECOLI

Accession

Primary (citable) accession number: P33361
Secondary accession number(s): P76435, Q2MAU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program