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Protein

Glycine betaine uptake system permease protein YehY

Gene

yehY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Probably responsible for the translocation of the substrate across the membrane.1 Publication

Caution

Was originally thought to be part of an osmoprotectant uptake system (PubMed:15251200). However, it was shown later that the complex does not mediate osmotic stress protection (PubMed:26325238).2 Publications

GO - Molecular functioni

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • glycine betaine transport Source: EcoCyc
  • transmembrane transport Source: InterPro

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEHY-MONOMER
MetaCyc:YEHY-MONOMER

Protein family/group databases

TCDBi3.A.1.12.15 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine uptake system permease protein YehYCurated
Gene namesi
Name:yehY
Ordered Locus Names:b2130, JW2118
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12011 yehY

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicCurated5
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Topological domaini27 – 44PeriplasmicCuratedAdd BLAST18
Transmembranei45 – 65HelicalSequence analysisAdd BLAST21
Topological domaini66 – 69CytoplasmicCurated4
Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Topological domaini91 – 106PeriplasmicCuratedAdd BLAST16
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 137CytoplasmicCurated10
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 180PeriplasmicCuratedAdd BLAST22
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 224CytoplasmicCuratedAdd BLAST23
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 247PeriplasmicCurated2
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Topological domaini269 – 322CytoplasmicCuratedAdd BLAST54
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Topological domaini344 – 345PeriplasmicCurated2
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 385Cytoplasmic1 PublicationAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000602561 – 385Glycine betaine uptake system permease protein YehYAdd BLAST385

Proteomic databases

PaxDbiP33361
PRIDEiP33361

Expressioni

Inductioni

Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ).Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
bolAP0ABE23EBI-545876,EBI-545774

Protein-protein interaction databases

BioGridi4260449, 12 interactors
DIPiDIP-11913N
IntActiP33361, 2 interactors
STRINGi316385.ECDH10B_2286

Structurei

3D structure databases

ProteinModelPortaliP33361
SMRiP33361
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 372ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST196

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107T5X Bacteria
COG1174 LUCA
HOGENOMiHOG000218077
InParanoidiP33361
KOiK05846
OMAiSPGSGLW

Family and domain databases

CDDicd06261 TM_PBP2, 1 hit
Gene3Di1.10.3720.10, 1 hit
InterProiView protein in InterPro
IPR000515 MetI-like
IPR035906 MetI-like_sf
PfamiView protein in Pfam
PF00528 BPD_transp_1, 1 hit
SUPFAMiSSF161098 SSF161098, 1 hit
PROSITEiView protein in PROSITE
PS50928 ABC_TM1, 1 hit

Sequencei

Sequence statusi: Complete.

P33361-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYFRINPVL ALLLLLTAIA AALPFISYAP NRLVSGEGRH LWQLWPQTIW
60 70 80 90 100
MLVGVGCAWL TACFIPGKKG SICALILAQF VFVLLVWGAG KAATQLAQNG
110 120 130 140 150
SALARTSLGS GFWLAAALAL LACSDAIRRI STHPLWRWLL HMQIAIIPLW
160 170 180 190 200
LLYSGTLNDL SLMKEYANRQ DVFDDALAQH LTLLFGAVLP ALVIGVPLGI
210 220 230 240 250
WCYFSTARQG AIFSLLNVIQ TVPSVALFGL LIAPLAALVT AFPWLGTLGI
260 270 280 290 300
AGTGMTPALI ALVLYALLPL VRGVVVGLNQ IPRDVLESAR AMGMSGAQRF
310 320 330 340 350
LHVQLPLALP VFLRSLRVVM VQTVGMAVIA ALIGAGGFGA LVFQGLLSSA
360 370 380
IDLVLLGVIP VIVLAVLTDA LFDLLIALLK VKRND
Length:385
Mass (Da):41,139
Last modified:November 1, 1997 - v2
Checksum:iE57055E3A2B141CC
GO

Sequence cautioni

The sequence AAA60493 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti346 – 385LLSSA…VKRND → AAKQRH in AAA60493 (Ref. 1) CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA Translation: AAA60493.1 Different initiation.
U00096 Genomic DNA Translation: AAC75191.1
AP009048 Genomic DNA Translation: BAE76607.1
PIRiA64981
RefSeqiNP_416634.1, NC_000913.3
WP_000220837.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75191; AAC75191; b2130
BAE76607; BAE76607; BAE76607
GeneIDi946660
KEGGiecj:JW2118
eco:b2130
PATRICifig|1411691.4.peg.114

Similar proteinsi

Entry informationi

Entry nameiYEHY_ECOLI
AccessioniPrimary (citable) accession number: P33361
Secondary accession number(s): P76435, Q2MAU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health