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Protein

Glycine betaine uptake system permease protein YehW

Gene

yehW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Probably responsible for the translocation of the substrate across the membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • glycine betaine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEHW-MONOMER.
ECOL316407:JW2116-MONOMER.
MetaCyc:YEHW-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.15. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine uptake system permease protein YehWCurated
Gene namesi
Name:yehW
Ordered Locus Names:b2128, JW2116
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12009. yehW.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicCurated8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 46PeriplasmicCuratedAdd BLAST17
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21
Topological domaini68 – 101CytoplasmicCuratedAdd BLAST34
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123PeriplasmicCurated1
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 161CytoplasmicCuratedAdd BLAST17
Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
Topological domaini183 – 206PeriplasmicCuratedAdd BLAST24
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 243Cytoplasmic1 PublicationAdd BLAST16

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000602551 – 243Glycine betaine uptake system permease protein YehWAdd BLAST243

Proteomic databases

PaxDbiP33359.
PRIDEiP33359.

Expressioni

Inductioni

Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ).Curated

Protein-protein interaction databases

STRINGi511145.b2128.

Structurei

3D structure databases

ProteinModelPortaliP33359.
SMRiP33359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 231ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST182

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105WJV. Bacteria.
COG1174. LUCA.
HOGENOMiHOG000012909.
InParanoidiP33359.
KOiK05846.
OMAiPIFENTV.
PhylomeDBiP33359.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMLRDPLFW LIALFVALIF WLPYSQPLFA ALFPQLPRPV YQQESFAALA
60 70 80 90 100
LAHFWLVGIS SLFAVIIGTG AGIAVTRPWG AEFRPLVETI AAVGQTFPPV
110 120 130 140 150
AVLAIAVPVI GFGLQPAIIA LILYGVLPVL QATLAGLGAI DASVTEVAKG
160 170 180 190 200
MGMSRGQRVR KVELPLAAPV ILAGVRTSVI INIGTATIAS TVGASTLGTP
210 220 230 240
IIIGLSGFNT AYVIQGALLV ALAAIIADRL FERLVQALSQ HAK
Length:243
Mass (Da):25,515
Last modified:February 1, 1994 - v1
Checksum:i16D90AD1338083DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60491.1.
U00096 Genomic DNA. Translation: AAC75189.1.
AP009048 Genomic DNA. Translation: BAE76605.1.
PIRiG64980.
RefSeqiNP_416632.1. NC_000913.3.
WP_000783123.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75189; AAC75189; b2128.
BAE76605; BAE76605; BAE76605.
GeneIDi949028.
KEGGiecj:JW2116.
eco:b2128.
PATRICi32119595. VBIEscCol129921_2208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60491.1.
U00096 Genomic DNA. Translation: AAC75189.1.
AP009048 Genomic DNA. Translation: BAE76605.1.
PIRiG64980.
RefSeqiNP_416632.1. NC_000913.3.
WP_000783123.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33359.
SMRiP33359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b2128.

Protein family/group databases

TCDBi3.A.1.12.15. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP33359.
PRIDEiP33359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75189; AAC75189; b2128.
BAE76605; BAE76605; BAE76605.
GeneIDi949028.
KEGGiecj:JW2116.
eco:b2128.
PATRICi32119595. VBIEscCol129921_2208.

Organism-specific databases

EchoBASEiEB1947.
EcoGeneiEG12009. yehW.

Phylogenomic databases

eggNOGiENOG4105WJV. Bacteria.
COG1174. LUCA.
HOGENOMiHOG000012909.
InParanoidiP33359.
KOiK05846.
OMAiPIFENTV.
PhylomeDBiP33359.

Enzyme and pathway databases

BioCyciEcoCyc:YEHW-MONOMER.
ECOL316407:JW2116-MONOMER.
MetaCyc:YEHW-MONOMER.

Miscellaneous databases

PROiP33359.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYEHW_ECOLI
AccessioniPrimary (citable) accession number: P33359
Secondary accession number(s): Q2MAV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be part of an osmoprotectant uptake system (PubMed:15251200). However, it was shown later that the complex does not mediate osmotic stress protection (PubMed:26325238).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.