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Protein

HTH-type transcriptional regulator MlrA

Gene

mlrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates transcription of csgD, the master regulator of biofilm formation, by binding to its promoter region (PubMed:11489123, PubMed:20874755). Also controls the transcription of cadC and ibaG (PubMed:20874755). Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA (PubMed:23708798).3 Publications

Enzyme regulationi

Activity is regulated by DgcM and PdeR.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi6 – 25H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG12008-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator MlrACurated
Alternative name(s):
MerR-like regulator A1 Publication
Gene namesi
Name:mlrA1 Publication
Synonyms:yehV
Ordered Locus Names:b2127, JW2115
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12008 mlrA

Pathology & Biotechi

Disruption phenotypei

Knockout mutant no longer produces curli, is mannose-resistant haemagglutination (MRHA) negative and produces smooth white colonies on Congo red agar.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981441 – 243HTH-type transcriptional regulator MlrAAdd BLAST243

Proteomic databases

PaxDbiP33358
PRIDEiP33358

Interactioni

Subunit structurei

Interacts with DgcM and PdeR.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260445, 16 interactors
IntActiP33358, 8 interactors
MINTiP33358
STRINGi316385.ECDH10B_2282

Structurei

3D structure databases

ProteinModelPortaliP33358
SMRiP33358
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 72HTH merR-typePROSITE-ProRule annotationAdd BLAST70

Phylogenomic databases

eggNOGiENOG4108XDN Bacteria
COG0789 LUCA
HOGENOMiHOG000119656
InParanoidiP33358
KOiK21089
OMAiHYLQSNN

Family and domain databases

InterProiView protein in InterPro
IPR009061 DNA-bd_dom_put_sf
IPR000551 MerR-type_HTH_dom
PfamiView protein in Pfam
PF13411 MerR_1, 1 hit
SMARTiView protein in SMART
SM00422 HTH_MERR, 1 hit
SUPFAMiSSF46955 SSF46955, 1 hit
PROSITEiView protein in PROSITE
PS00552 HTH_MERR_1, 1 hit
PS50937 HTH_MERR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALYTIGEVA LLCDINPVTL RAWQRRYGLL KPQRTDGGHR LFNDADIDRI
60 70 80 90 100
REIKRWIDNG VQVSKVKMLL SNENVDVQNG WRDQQETLLT YLQSGNLHSL
110 120 130 140 150
RTWIKERGQD YPAQTLTTHL FIPLRRRLQC QQPTLQALLA ILDGVLINYI
160 170 180 190 200
AICLASARKK QGKDALVVGW NIQDTTRLWL EGWIASQQGW RIDVLAHSLN
210 220 230 240
QLRPELFEGR TLLVWCGENR TSAQQQQLTS WQEQGHDIFP LGI
Length:243
Mass (Da):28,046
Last modified:February 1, 1994 - v1
Checksum:i48F6D16051C04319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288173 Genomic DNA Translation: AAK84657.1
AF288174 Genomic DNA Translation: AAK84658.1
U00007 Genomic DNA Translation: AAA60490.1
U00096 Genomic DNA Translation: AAC75188.1
AP009048 Genomic DNA Translation: BAE76603.1
PIRiF64980
RefSeqiNP_416631.1, NC_000913.3
WP_001240401.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75188; AAC75188; b2127
BAE76603; BAE76603; BAE76603
GeneIDi949029
KEGGiecj:JW2115
eco:b2127
PATRICifig|1411691.4.peg.118

Entry informationi

Entry nameiMLRA_ECOLI
AccessioniPrimary (citable) accession number: P33358
Secondary accession number(s): Q2MAV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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