P33358 (MLRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator mlrA

Alternative name(s):
MerR-like regulator A

Gene names

Name:	mlrA
Synonyms:	yehV
Ordered Locus Names:	b2127, JW2115

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Transcriptional activator of the csg genes required for production of the curli (AgF). Ref.1
Sequence similarities	Contains 1 HTH merR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	HTH-type transcriptional regulator mlrA		PRO_0000098144
Regions
	Domain	3 – 72	70	HTH merR-type
	DNA binding	6 – 25	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P33358 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 48F6D16051C04319	FASTA	243	28,046
	10 20 30 40 50 60 MALYTIGEVA LLCDINPVTL RAWQRRYGLL KPQRTDGGHR LFNDADIDRI REIKRWIDNG 70 80 90 100 110 120 VQVSKVKMLL SNENVDVQNG WRDQQETLLT YLQSGNLHSL RTWIKERGQD YPAQTLTTHL 130 140 150 160 170 180 FIPLRRRLQC QQPTLQALLA ILDGVLINYI AICLASARKK QGKDALVVGW NIQDTTRLWL 190 200 210 220 230 240 EGWIASQQGW RIDVLAHSLN QLRPELFEGR TLLVWCGENR TSAQQQQLTS WQEQGHDIFP LGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium." Brown P.K., Dozois C.M., Nickerson C.A., Zuppardo A., Terlonge J., Curtiss R. III Mol. Microbiol. 41:349-363(2001) [PubMed: 11489123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 33694 / HB101 and Chi7122.
[2]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288173 Genomic DNA. Translation: AAK84657.1. AF288174 Genomic DNA. Translation: AAK84658.1. U00007 Genomic DNA. Translation: AAA60490.1. U00096 Genomic DNA. Translation: AAC75188.1. AP009048 Genomic DNA. Translation: BAE76603.1.
PIR	F64980.
RefSeq	NP_416631.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P33358.
SMR	P33358. Positions 3-73.
ModBase	Search...
Protein-protein interaction databases
IntAct	P33358. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000508; EBESCP00000000508; EBESCG00000000426. EBESCT00000016764; EBESCP00000016055; EBESCG00000015823.
GeneID	949029.
GenomeReviews	Gene locus JW2115 in contig AP009048_GR. Gene locus b2127 in contig U00096_GR.
KEGG	ecj:JW2115. eco:b2127.
PATRIC	32119591. VBIEscCol129921_2206.
Organism-specific databases
EchoBASE	EB1946.
EcoGene	EG12008. mlrA.
Phylogenomic databases
eggNOG	COG0789.
GeneTree	EBGT00050000010907.
HOGENOM	HBG417247.
OMA	WLEAWIA.
PhylomeDB	P33358.
ProtClustDB	PRK15043.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12008-MONOMER.
Gene expression databases
Genevestigator	P33358.
Family and domain databases
InterPro	IPR009061. DNA-bd_dom_put. IPR000551. Tscrpt_reg_HTH_MerR. [Graphical view]
Pfam	PF00376. MerR. 1 hit. [Graphical view]
SMART	SM00422. HTH_MERR. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 1 hit.
PROSITE	PS00552. HTH_MERR_1. 1 hit. PS50937. HTH_MERR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MLRA_ECOLI

Accession

Primary (citable) accession number: P33358
Secondary accession number(s): Q2MAV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program