P33345 (MOLR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative molybdate metabolism regulator

Gene names

Name:	yehH
Synonyms:	molR
Ordered Locus Names:	b4499
ORF Names:	b2115/b2116/b2117

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	1264 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Was originally thought to be involved in the regulation of molybdate metabolism (Ref.3), has also been implicated in selenate reduction (Ref.5).
Sequence similarities	To E.coli YehI.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AAA60477.1 differs from that shown. Reason: Frameshift at positions 275 and 950. The sequence U00096 differs from that shown. Reason: Frameshift at positions 275 and 950.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1264	1264	Putative molybdate metabolism regulator		PRO_0000096531

Sequences

	Sequence		Length	Mass (Da)
	P33345 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FCB85A5933CCEFF4	FASTA	1,264	140,939
	10 20 30 40 50 60 MRHFIYQDEK SHKFRAVEQQ GNELHISWGK VGTKGQSQIK SFSDAAAAAK AELKLIAEKV 70 80 90 100 110 120 KKGYVEQAKD NSLQPSQTVT GSLKVADLST IIQEQPSFVA ETRAPDKNTD AVLPWLAKDI 130 140 150 160 170 180 AVVFPPEVVH TTLSHRRFPG VPVQQADKLP QLRRLACSVS QRDNKTATFD FSACSLEWQN 190 200 210 220 230 240 TVAQAISQID GLKTTQLPSP VMAVLTALEM KCTRYKVRED VMDQIVQEGG LEYATDVIIH 250 260 270 280 290 300 LQQIDIEWDY ANNVIIILPS GIAPSYLEQY SRFEXRLRKH LSLTEESLWQ KCAQKLIAAI 310 320 330 340 350 360 PHIPEWRQPL IALLLPEKPE IAHEIAQRLL GQKKLPSLEW LKIVATDEHI LASLEKYHEP 370 380 390 400 410 420 YAIFDDYYCG AIWSATVLQE QGVAALPRFA PYAASDYCAD VLRHINHPFA LTLLIRVAGQ 430 440 450 460 470 480 TKRCHDRMTK AIAAFPHAAM AALTELLGQK EENSWRIMLM TMLISQPALA EQVIPWLSTP 490 500 510 520 530 540 AVAVLKSCQQ QLTQPSNHAS ADLLPAVVVS PPWLSKKKKS PIPVLDLAPL GIEPICYLTE 550 560 570 580 590 600 EISNQLLAKY IWYSKHITVS HEESTTNLLA RMGFQRRIAG TYIKAPEAVV EAWLNEDYST 610 620 630 640 650 660 LLSEFKVFHS PTGHYWQLGI LTTLPLEKAV KAWNALTLSP HTDTEYSMLH FGLKGLPGLV 670 680 690 700 710 720 NSLARYPQEA LPITNYFAAS ELAPAVARAF NKLKTLRENA RSWLLKYPEH ALTGLLPAAP 730 740 750 760 770 780 GKAGEAQDNA RAALRMLTEN GHQPLLQEIA RRYNQPEVTD AVNALLALDP LDNHPTKIPT 790 800 810 820 830 840 LPAFYQPSLW TRPVLKANAQ SLPDSALLHL GEMLRFPQEE ALYPGLLQVK DVCSADSLAG 850 860 870 880 890 900 FAWDLFTAWQ TAGAPSKESW AFTALGVLGN DDTARKLTPL IRAWPGESQH KRATVGLDIL 910 920 930 940 950 960 AAIGSDIALM QLNGIAQKLK FKALQERAKE KIADIAESRE LTVAELEDRL APDLGLDDNG 970 980 990 1000 1010 1020 SLLLDFGPRQ FTVSFDETLK PFVRDVSGSR LKDLPKPNKS DDETRANDAV NRYKLLKKDA 1030 1040 1050 1060 1070 1080 RTIAAQQVAR LESAMCLRRR WSLENFQLFL VEHPLVRHLT RRLIWGVYSA ENQLLACFRV 1090 1100 1110 1120 1130 1140 AEDNSSSTAD DDLFTLPEGD ISIGTPHVLE ISPTDAAAFG QLFADYELLP PFRQLDRNSY 1150 1160 1170 1180 1190 1200 ALTEAERNAS ELTRWAGRKC PSGRVMGLAN KGWIKGEPQD GGWIGWMIKP LGRWSLIMEI 1210 1220 1230 1240 1250 1260 DEGFAVGMSP AELSAEQLLS KLWLWEGKAE RYGWGSNSTQ EAQFSVIDAI TASELINDIE ALFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli." Lee J.H., Wendt J.C., Shanmugam K.T. J. Bacteriol. 172:2079-2087(1990) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / BW545.
[4]	Healy F., Grunden A., Ray R.M., Shanmugam K.T. Unpublished observations (FEB-1994) Cited for: CHARACTERIZATION.
[5]	"Involvement of a putative molybdenum enzyme in the reduction of selenate by Escherichia coli." Bebien M., Kirsch J., Mejean V., Vermeglio A. Microbiology 148:3865-3872(2002) [PubMed] [Europe PMC] [Abstract] Cited for: POTENTIAL INVOLVEMENT IN SELENATE REDUCTION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00007 Genomic DNA. Translation: AAA60477.1. Frameshift. U00096 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	P33345.
SMR	P33345. Positions 2-70.
ModBase	Search...
Protein-protein interaction databases
IntAct	P33345. 8 interactions.
Proteomic databases
PRIDE	P33345.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
EchoBASE	EB1935.
EcoGene	EG11992. yehH.
Phylogenomic databases
HOGENOM	HOG000122164.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11992-MONOMER. EcoCyc:G8208-MONOMER. EcoCyc:G8209-MONOMER.
Gene expression databases
Genevestigator	P33345.
Family and domain databases
Gene3D	2.20.140.10. 1 hit.
InterPro	IPR025406. DUF4132. IPR008893. WGR_domain. [Graphical view]
Pfam	PF13569. DUF4132. 1 hit. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF142921. SSF142921. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MOLR_ECOLI

Accession

Primary (citable) accession number: P33345
Secondary accession number(s): P76427, P76428, P76429

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program