P33339 (TFC4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor tau 131 kDa subunit

Alternative name(s):
TFIIIC 131 kDa subunit
Transcription factor C subunit 4

Gene names

Name:	TFC4
Synonyms:	PCF1
Ordered Locus Names:	YGR047C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. TFC4 is the TFIIIB-assembling subunit of TFIIIC and essential for viability. Ref.1 Ref.8
Subunit structure	Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC1, TFC3, TFC6, TFIIIB subunits BRF1 and BDP1, and with RNA polymerase III subunit RPC10. Ref.10 Ref.11 Ref.13 Ref.14
Subcellular location	Nucleus Ref.15.
Post-translational modification	Phosphorylated. Ref.11
Miscellaneous	Present with 876 molecules/cell in log phase SD medium. Ref.16
Sequence similarities	Contains 11 TPR repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat TPR repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase III promoter Traceable author statement. Source: SGD
Cellular component	transcription factor TFIIIC complex Inferred from direct assay Ref.9Ref.1. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	Transcription factor tau 131 kDa subunit		PRO_0000106364
Regions
	Repeat	128 – 161	34	TPR 1
	Repeat	162 – 195	34	TPR 2
	Repeat	196 – 229	34	TPR 3
	Repeat	230 – 263	34	TPR 4
	Repeat	264 – 297	34	TPR 5
	Repeat	432 – 465	34	TPR 6
	Repeat	467 – 501	35	TPR 7
	Repeat	502 – 535	34	TPR 8
	Repeat	536 – 569	34	TPR 9
	Repeat	875 – 908	34	TPR 10
	Repeat	959 – 992	34	TPR 11
	Region	128 – 569	442	Sufficient to bind BDP1
Natural variations
	Natural variant	280	1	I → T in strain: SK1. Ref.5
	Natural variant	635	1	M → V in strain: SK1. Ref.5
	Natural variant	1025	1	I → V in strain: SK1. Ref.5
Experimental info
	Mutagenesis	148	1	E → K in PCF1-17; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	162	1	F → L in PCF1-12; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	162	1	F → S in PCF1-139; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	164	1	A → V in PCF1-19; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	167	1	T → I in PCF1-2; increases RNA polymerase III gene transcription due to an increase in the recruitment of BRF1 to TFIIIC-DNA. No affect on affinity of TFIIIC for DNA. Ref.3
	Mutagenesis	172	1	Y → C in PCF1-11; increases RNA polymerase III gene transcription.
	Mutagenesis	188	1	A → T in PCF1-23; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	190	1	H → Y in PCF1-1; affects the rate of recruitment of TFIIIB to the template. Increases the amount of transcriptionally active TFIIIB. Increases RNA polymerase III gene transcription. Increases the binding affinity for BRF1, but does not affect the binding affinity for BDP1 in the TFIIIC-dependent assembly of TFIIIB. Overcomes autoinhibition of BRF1 binding. Ref.2 Ref.3 Ref.4 Ref.12
	Mutagenesis	192	1	N → L in PCF1-138; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	199	1	W → R in PCF1-15; increases RNA polymerase III gene transcription. Ref.3
	Mutagenesis	469	1	L → K: RNA polymerase III defective. Defect in the recruitment of BRF1 into TFIIIB-TFIIIC-DNA complexes and diminished direct interaction between TFC4 and BRF1. Decreased binding affinity for BDP1 incorporation into TFIIIB-TFIIIC-DNA complexes and inhibited binary interaction between BDP1 and TFC4. Ref.14
	Mutagenesis	472	1	E → K: RNA polymerase III defective. Ref.14
	Mutagenesis	504	1	V → K: RNA polymerase III defective. Ref.14
	Mutagenesis	541	1	S → I: RNA polymerase III defective. Ref.14
	Mutagenesis	542	1	L → G: RNA polymerase III defective. Ref.14
	Mutagenesis	728	1	R → E in PCF1-8; increases RNA polymerase III transcription. Ref.12
	Mutagenesis	728	1	R → G in PCF1-7; increases RNA polymerase III transcription. Ref.12
	Mutagenesis	728	1	R → H in PCF1-4; increases RNA polymerase III transcription ninefold over wild-type. Increases the amount of transcriptionally active TFIIIB. Ref.12
	Mutagenesis	728	1	R → K in PCF1-3; increases RNA polymerase III transcription two- to threefold over wild-type. Increases the amount of transcriptionally active TFIIIB. Ref.12
	Mutagenesis	728	1	R → M in PCF1-5; increases RNA polymerase III transcription. Ref.12
	Mutagenesis	728	1	R → V in PCF1-6; increases RNA polymerase III transcription. Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P33339 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 12DE18304AAF4862	FASTA	1,025	120,229
	10 20 30 40 50 60 MAAGKLKKEQ QNQSAERESA DTGKVNDEDE EHLYGNIDDY KHLIQDEEYD DEDVPHDLQL 70 80 90 100 110 120 SEDEYNSERD SSLLAEFSDY GEISEDDEED FMNAIREASN FKVKKKKKND KGKSYGRQRK 130 140 150 160 170 180 ERVLDPEVAQ LLSQANEAFV RNDLQVAERL FNEVIKKDAR NFAAYETLGD IYQLQGRLND 190 200 210 220 230 240 CCNSWFLAAH LNASDWEFWK IVAILSADLD HVRQAIYCFS RVISLNPMEW ESIYRRSMLY 250 260 270 280 290 300 KKTGQLARAL DGFQRLYMYN PYDANILREL AILYVDYDRI EDSIELYMKV FNANVERREA 310 320 330 340 350 360 ILAALENALD SSDEESAAEG EDADEKEPLE QDEDRQMFPD INWKKIDAKY KCIPFDWSSL 370 380 390 400 410 420 NILAELFLKL AVSEVDGIKT IKKCARWIQR RESQTFWDHV PDDSEFDNRR FKNSTFDSLL 430 440 450 460 470 480 AAEKEKSYNI PIDIRVRLGL LRLNTDNLVE ALNHFQCLYD ETFSDVADLY FEAATALTRA 490 500 510 520 530 540 EKYKEAIDFF TPLLSLEEWR TTDVFKPLAR CYKEIESYET AKEFYELAIK SEPDDLDIRV 550 560 570 580 590 600 SLAEVYYRLN DPETFKHMLV DVVEMRKHQV DETLHRISNE KSSNDTSDIS SKPLLEDSKF 610 620 630 640 650 660 RTFRKKKRTP YDAERERIER ERRITAKVVD KYEKMKKFEL NSGLNEAKQA SIWINTVSEL 670 680 690 700 710 720 VDIFSSVKNF FMKSRSRKFV GILRRTKKFN TELDFQIERL SKLAEGDSVF EGPLMEERVT 730 740 750 760 770 780 LTSATELRGL SYEQWFELFM ELSLVIAKYQ SVEDGLSVVE TAQEVNVFFQ DPERVKMMKF 790 800 810 820 830 840 VKLAIVLQMD DEEELAENLR GLLNQFQFNR KVLQVFMYSL CRGPSSLNIL SSTIQQKFFL 850 860 870 880 890 900 RQLKAFDSCR YNTEVNGQAS ITNKEVYNPN KKSSPYLYYI YAVLLYSSRG FLSALQYLTR 910 920 930 940 950 960 LEEDIPDDPM VNLLMGLSHI HRAMQRLTAQ RHFQIFHGLR YLYRYHKIRK SLYTDLEKQE 970 980 990 1000 1010 1020 ADYNLGRAFH LIGLVSIAIE YYNRVLENYD DGKLKKHAAY NSIIIYQQSG NVELADHLME KYLSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The TFIIIB-assembling subunit of yeast transcription factor TFIIIC has both tetratricopeptide repeats and basic helix-loop-helix motifs." Marck C., Lefebvre O., Carles C., Riva M., Chaussivert N., Ruet A., Sentenac A. Proc. Natl. Acad. Sci. U.S.A. 90:4027-4031(1993) [PubMed: 8387209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN TFIIIC. Strain: ATCC 204508 / S288c.
[2]	"A mutation in the second largest subunit of TFIIIC increases a rate-limiting step in transcription by RNA polymerase III." Rameau G., Puglia K., Crowe A., Sethy I., Willis I. Mol. Cell. Biol. 14:822-830(1994) [PubMed: 8264649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN TFIIIC, MUTAGENESIS OF HIS-190.
[3]	"A tetratricopeptide repeat mutation in yeast transcription factor IIIC131 (TFIIIC131) facilitates recruitment of TFIIB-related factor TFIIIB70." Moir R.D., Sethy-Coraci I., Puglia K., Librizzi M.D., Willis I.M. Mol. Cell. Biol. 17:7119-7125(1997) [PubMed: 9372943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 147-205, MUTAGENESIS OF GLU-148; PHE-162; ALA-164; THR-167; TYR-171; ALA-188; HIS-190; ASN-192 AND TRP-199.
[4]	"A gain-of-function mutation in the second tetratricopeptide repeat of TFIIIC131 relieves autoinhibition of Brf1 binding." Moir R.D., Puglia K.V., Willis I.M. Mol. Cell. Biol. 22:6131-6141(2002) [PubMed: 12167707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-190.
[5]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed: 16273108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-280; VAL-635 AND VAL-1025. Strain: SK1.
[6]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[7]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[8]	"A selection for mutants of the RNA polymerase III transcription apparatus: PCF1 stimulates transcription of tRNA and 5S RNA genes." Willis I., Schmidt P., Soell D. EMBO J. 8:4281-4288(1989) [PubMed: 2686985] [Abstract] Cited for: FUNCTION.
[9]	"Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies." Parsons M.C., Weil P.A. J. Biol. Chem. 265:5095-5103(1990) [PubMed: 2180956] [Abstract] Cited for: IDENTIFICATION IN TFIIIC.
[10]	"Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC." Bartholomew B., Kassavetis G.A., Geiduschek E.P. Mol. Cell. Biol. 11:5181-5189(1991) [PubMed: 1922038] [Abstract] Cited for: INTERACTION WITH TFIIIB.
[11]	"On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau." Conesa C., Swanson R.N., Schultz P., Oudet P., Sentenac A. J. Biol. Chem. 268:18047-18052(1993) [PubMed: 7688737] [Abstract] Cited for: INTERACTION WITH TFC1; TFC3 AND TFC6, PHOSPHORYLATION.
[12]	"Recessive mutations in the second largest subunit of TFIIIC suggest a new step in RNA polymerase III transcription." Sethy I., Willis I.M. Gene Expr. 5:35-47(1995) [PubMed: 7488859] [Abstract] Cited for: MUTAGENESIS OF HIS-190 AND ARG-728.
[13]	"Interaction between yeast RNA polymerase III and transcription factor TFIIIC via ABC10alpha and tau131 subunits." Dumay H., Rubbi L., Sentenac A., Marck C. J. Biol. Chem. 274:33462-33468(1999) [PubMed: 10559229] [Abstract] Cited for: INTERACTION WITH RPC10.
[14]	"The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4." Liao Y., Willis I.M., Moir R.D. J. Biol. Chem. 278:44467-44474(2003) [PubMed: 12930823] [Abstract] Cited for: INTERACTION WITH BRF1 AND BDP1, MUTAGENESIS OF LEU-469; GLU-472; VAL-504; SER-541 AND LEU-542.
[15]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[16]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12722 Genomic DNA. Translation: AAA35145.1. DQ115391 Genomic DNA. Translation: AAZ22462.1. Z72832 Genomic DNA. Translation: CAA97046.1. Z72833 Genomic DNA. Translation: CAA97048.1. BK006941 Genomic DNA. Translation: DAA08145.1.
PIR	A47453.
RefSeq	NP_011561.1. NM_001181176.1.
3D structure databases
ProteinModelPortal	P33339.
SMR	P33339. Positions 132-297, 439-551.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-230N.
IntAct	P33339. 16 interactions.
MINT	MINT-402608.
STRING	P33339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR047C; YGR047C; YGR047C.
GeneID	852938.
KEGG	sce:YGR047C.
NMPDR	fig|4932.3.peg.2677.
Organism-specific databases
CYGD	YGR047c.
SGD	S000003279. TFC4.
Phylogenomic databases
eggNOG	fuNOG07762.
GeneTree	EFGT00050000004377.
HOGENOM	HBG396623.
OMA	YLEAYKL.
OrthoDB	EOG4R539Z.
Enzyme and pathway databases
Reactome	REACT_87991. Transcription.
Gene expression databases
ArrayExpress	P33339.
Genevestigator	P33339.
GermOnline	YGR047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 4 hits.
KO	K15201.
SMART	SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS50005. TPR. 6 hits. PS50293. TPR_REGION. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	972675.

Entry information

Entry name

TFC4_YEAST

Accession

Primary (citable) accession number: P33339
Secondary accession number(s): D6VUI4, Q45U37

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program