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Protein

Protein SGE1

Gene

SGE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Drug export permease. Multi-copy suppressor of loss-of-function mutation of GAL11. Involved specifically in transcription of GAL4-dependent genes. Can link GAL4 with the basal transcription machinery if GAL11 is missing. Confers resistance to 10-N-nonyl acridine orange (NAO) and in general to cationic dyes.

GO - Molecular functioni

  • drug transmembrane transporter activity Source: SGD

GO - Biological processi

  • drug transmembrane transport Source: GOC
  • drug transport Source: SGD
  • response to drug Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34318-MONOMER.

Protein family/group databases

TCDBi2.A.1.3.54. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SGE1
Alternative name(s):
10-N-nonyl acridine orange resistance protein
Crystal violet resistance protein
Gene namesi
Name:SGE1
Synonyms:NOR1
Ordered Locus Names:YPR198W
ORF Names:P9677.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR198W.
SGDiS000006402. SGE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 4112ExtracellularSequence analysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Topological domaini63 – 7917CytoplasmicSequence analysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Topological domaini101 – 1033ExtracellularSequence analysis
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 1317CytoplasmicSequence analysis
Transmembranei132 – 15221HelicalSequence analysisAdd
BLAST
Topological domaini153 – 16210ExtracellularSequence analysis
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Topological domaini184 – 22744CytoplasmicSequence analysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence analysisAdd
BLAST
Topological domaini249 – 2557ExtracellularSequence analysis
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Topological domaini277 – 30024CytoplasmicSequence analysisAdd
BLAST
Transmembranei301 – 32121HelicalSequence analysisAdd
BLAST
Topological domaini322 – 34120ExtracellularSequence analysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 37311CytoplasmicSequence analysisAdd
BLAST
Transmembranei374 – 39421HelicalSequence analysisAdd
BLAST
Topological domaini395 – 3995ExtracellularSequence analysis
Transmembranei400 – 42021HelicalSequence analysisAdd
BLAST
Topological domaini421 – 44323CytoplasmicSequence analysisAdd
BLAST
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 50844ExtracellularSequence analysisAdd
BLAST
Transmembranei509 – 52921HelicalSequence analysisAdd
BLAST
Topological domaini530 – 54314CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Protein SGE1PRO_0000173422Add
BLAST

Proteomic databases

MaxQBiP33335.

Interactioni

Protein-protein interaction databases

BioGridi36368. 53 interactions.
DIPiDIP-7575N.
IntActiP33335. 3 interactions.
MINTiMINT-1363018.

Structurei

3D structure databases

ProteinModelPortaliP33335.
SMRiP33335. Positions 20-227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP33335.
OMAiGSTIMAT.
OrthoDBiEOG769ZTJ.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSTLSLTLC VISLLLTLFL AALDIVIVVT LYDTIGIKFH DFGNIGWLVT
60 70 80 90 100
GYALSNAVFM LLWGRLAEIL GTKECLMISV IVFEIGSLIS ALSNSMATLI
110 120 130 140 150
SGRVVAGFGG SGIESLAFVV GTSIVRENHR GIMITALAIS YVIAEGVGPF
160 170 180 190 200
IGGAFNEHLS WRWCFYINLP IGAFAFIILA FCNTSGEPHQ KMWLPSKIKK
210 220 230 240 250
IMNYDYGELL KASFWKNTFE VLVFKLDMVG IILSSAGFTL LMLGLSFGGN
260 270 280 290 300
NFPWNSGIII CFFTVGPILL LLFCAYDFHF LSLSGLHYDN KRIKPLLTWN
310 320 330 340 350
IASNCGIFTS SITGFLSCFA YELQSAYLVQ LYQLVFKKKP TLASIHLWEL
360 370 380 390 400
SIPAMIATMA IAYLNSKYGI IKPAIVFGVL CGIVGSGLFT LINGELSQSI
410 420 430 440 450
GYSILPGIAF GSIFQATLLS SQVQITSDDP DFQNKFIEVT AFNSFAKSLG
460 470 480 490 500
FAFGGNMGAM IFTASLKNQM RSSQLNIPQF TSVETLLAYS TEHYDGPQSS
510 520 530 540
LSKFINTAIH DVFYCALGCY ALSFFFGIFT SSKKTTISAK KQQ
Length:543
Mass (Da):59,426
Last modified:February 1, 1994 - v1
Checksum:i16A3D91DA0B950FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti429 – 4291D → G in AAA65606 (Ref. 3) Curated
Sequence conflicti447 – 48640KSLGF…SVETL → NPWALRLEGYGGNDIHCITQ KPDALFPIKHTTIYVCRNT in AAA65606 (Ref. 3) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11640 Genomic DNA. Translation: AAA35043.1.
U02077 Genomic DNA. Translation: AAA52190.1.
L24961 Genomic DNA. Translation: AAA65606.1.
X77765 Genomic DNA. Translation: CAA54805.1.
U25841 Genomic DNA. Translation: AAB64626.1.
BK006949 Genomic DNA. Translation: DAA11612.1.
PIRiS40888.
RefSeqiNP_015524.1. NM_001184295.1.

Genome annotation databases

EnsemblFungiiYPR198W; YPR198W; YPR198W.
GeneIDi856327.
KEGGisce:YPR198W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11640 Genomic DNA. Translation: AAA35043.1.
U02077 Genomic DNA. Translation: AAA52190.1.
L24961 Genomic DNA. Translation: AAA65606.1.
X77765 Genomic DNA. Translation: CAA54805.1.
U25841 Genomic DNA. Translation: AAB64626.1.
BK006949 Genomic DNA. Translation: DAA11612.1.
PIRiS40888.
RefSeqiNP_015524.1. NM_001184295.1.

3D structure databases

ProteinModelPortaliP33335.
SMRiP33335. Positions 20-227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36368. 53 interactions.
DIPiDIP-7575N.
IntActiP33335. 3 interactions.
MINTiMINT-1363018.

Protein family/group databases

TCDBi2.A.1.3.54. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiP33335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR198W; YPR198W; YPR198W.
GeneIDi856327.
KEGGisce:YPR198W.

Organism-specific databases

EuPathDBiFungiDB:YPR198W.
SGDiS000006402. SGE1.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP33335.
OMAiGSTIMAT.
OrthoDBiEOG769ZTJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34318-MONOMER.

Miscellaneous databases

PROiP33335.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of SGE1: a yeast gene that partially suppresses the gal11 mutation in multiple copies."
    Amakasu H., Suzuki Y., Nishizawa M., Fukasawa T.
    Genetics 134:675-683(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae SGE1 gene product: a novel drug-resistance protein within the major facilitator superfamily."
    Ehrenhofer-Murray A.E., Wuergler F.E., Sengstag C.
    Mol. Gen. Genet. 244:287-294(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Membrane protein confering resistance to drug."
    Urdaci M.C., Jacquot C.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  4. "The Saccharomyces cerevisiae MFS superfamily SGE1 gene confers resistance to cationic dyes."
    Jacquot C., Julien R., Guilloton M.
    Yeast 13:891-902(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "The Sge1 protein of Saccharomyces cerevisiae is a membrane-associated multidrug transporter."
    Ehrenhofer-Murray A.E., Keller Seitz M.U., Sengstag C.
    Yeast 14:49-65(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSGE1_YEAST
AccessioniPrimary (citable) accession number: P33335
Secondary accession number(s): D6W4J6, Q05720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.