Reviewed,
UniProtKB/Swiss-Prot P33333 (PLSC_YEAST)
Last modified
February 9, 2010.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase Short name=1-AGP acyltransferase Short name=1-AGPAT EC=2.3.1.51 Alternative name(s): Lysophosphatidic acid acyltransferase Short name=LPAAT | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 303 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May be an acyltransferase with an altered substrate specificity that enables it to use a C-26-CoA in place of the C-16 or C-18-CoAs used by the wild-type protein. |
| Catalytic activity | Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. |
| Pathway | |
| Subcellular location | Membrane; Single-pass membrane protein Potential. |
| Domain | The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. |
| Sequence similarities | Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phospholipid biosynthesis |
| Cellular component | Membrane |
| Domain | Transmembrane |
| Molecular function | Acyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycerophospholipid biosynthetic process Inferred from genetic interaction. Source: SGD |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lipid particleInferred from direct assay. Source: SGD |
| Molecular function | 1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from direct assay. Source: SGD protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 303 | 303 | Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase | PRO_0000208189 | |||||
Regions | |||||||||
| Transmembrane | 16 – 30 | 15 | Potential | ||||||
| Motif | 82 – 87 | 6 | HXXXXD motif | ||||||
Natural variations | |||||||||
| Natural variant | 44 | 1 | Q → L in allele suppressor SLC1-1. | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase." Nagiec M.M., Wells G.B., Lester R.L., Dickson R.C. J. Biol. Chem. 268:22156-22163(1993) [PubMed: 8408076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.  Zaccaria P.Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L13282 Unassigned DNA. Translation: AAA16514.1. Z74100 Genomic DNA. Translation: CAA98614.1. |
| PIR | A48600. |
| RefSeq | NP_010231.1. |
3D structure databases | |
| SMR | P33333. Positions 67-224. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5136N. |
| IntAct | P33333. 59 interactions. |
| STRING | P33333. |
Proteomic databases | |
| PRIDE | P33333. |
Genome annotation databases | |
| Ensembl | YDL052C; YDL052C; YDL052C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 851508. |
| KEGG | sce:YDL052C. |
| NMPDR | fig|4932.3.peg.971. |
Organism-specific databases | |
| CYGD | YDL052c. |
| SGD | S000002210. SLC1. |
Phylogenomic databases | |
| eggNOG | fuNOG07013. |
| HOGENOM | HBG736534. |
| OMA | FNLCAYL. |
| OrthoDB | EOG9J3Z01. |
| PhylomeDB | P33333. |
Enzyme and pathway databases | |
| BRENDA | 2.3.1.51. 250. |
Gene expression databases | |
| ArrayExpress | P33333. |
| Genevestigator | P33333. |
| GermOnline | YDL052C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view] |
| Pfam | PF01553. Acyltransferase. 1 hit. [Graphical view] |
| SMART | SM00563. PlsC. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00530. AGP_acyltrn. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 968866. |
Entry information
| Entry name | PLSC_YEAST | ||||||||
| Accession | Primary (citable) accession number: P33333 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
