Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase

Gene

SLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be an acyltransferase with an altered substrate specificity that enables it to use a C-26-CoA in place of the C-16 or C-18-CoAs used by the wild-type protein.

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate O-acyltransferase 2 (GPT2), Glycerol-3-phosphate O-acyltransferase 1 (SCT1)
  2. Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase (SLC1)
  3. Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAM41)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: SGD

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • glycerophospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YDL052C-MONOMER.
YEAST:YDL052C-MONOMER.
BRENDAi2.3.1.51. 984.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-163765. ChREBP activates metabolic gene expression.
R-SCE-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Chemistry

SwissLipidsiSLP:000000049.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC:2.3.1.51)
Short name:
1-AGP acyltransferase
Short name:
1-AGPAT
Alternative name(s):
Lysophosphatidic acid acyltransferase
Short name:
LPAAT
Gene namesi
Name:SLC1
Ordered Locus Names:YDL052C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL052C.
SGDiS000002210. SLC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3015HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Probable 1-acyl-sn-glycerol-3-phosphate acyltransferasePRO_0000208189Add
BLAST

Proteomic databases

MaxQBiP33333.

Interactioni

Protein-protein interaction databases

BioGridi32007. 58 interactions.
DIPiDIP-5136N.
IntActiP33333. 40 interactions.
MINTiMINT-519109.

Structurei

3D structure databases

ProteinModelPortaliP33333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 876HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000008726.
HOGENOMiHOG000026375.
InParanoidiP33333.
KOiK13509.
OMAiPDYKSGI.
OrthoDBiEOG092C4MB5.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.

Sequencei

Sequence statusi: Complete.

P33333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIGRFLYY LRSVLVVLAL AGCGFYGVIA SILCTLIGKQ HLAQWITARC
60 70 80 90 100
FYHVMKLMLG LDVKVVGEEN LAKKPYIMIA NHQSTLDIFM LGRIFPPGCT
110 120 130 140 150
VTAKKSLKYV PFLGWFMALS GTYFLDRSKR QEAIDTLNKG LENVKKNKRA
160 170 180 190 200
LWVFPEGTRS YTSELTMLPF KKGAFHLAQQ GKIPIVPVVV SNTSTLVSPK
210 220 230 240 250
YGVFNRGCMI VRILKPISTE NLTKDKIGEF AEKVRDQMVD TLKEIGYSPA
260 270 280 290 300
INDTTLPPQA IEYAALQHDK KVNKKIKNEP VPSVSISNDV NTHNEGSSVK

KMH
Length:303
Mass (Da):33,887
Last modified:February 1, 1994 - v1
Checksum:i36ECBBC2659655EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151W → R in AAT92911 (PubMed:17322287).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441Q → L in allele suppressor SLC1-1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13282 Unassigned DNA. Translation: AAA16514.1.
Z74100 Genomic DNA. Translation: CAA98614.1.
AY692892 Genomic DNA. Translation: AAT92911.1.
BK006938 Genomic DNA. Translation: DAA11804.1.
PIRiA48600.
RefSeqiNP_010231.1. NM_001180111.1.

Genome annotation databases

EnsemblFungiiYDL052C; YDL052C; YDL052C.
GeneIDi851508.
KEGGisce:YDL052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13282 Unassigned DNA. Translation: AAA16514.1.
Z74100 Genomic DNA. Translation: CAA98614.1.
AY692892 Genomic DNA. Translation: AAT92911.1.
BK006938 Genomic DNA. Translation: DAA11804.1.
PIRiA48600.
RefSeqiNP_010231.1. NM_001180111.1.

3D structure databases

ProteinModelPortaliP33333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32007. 58 interactions.
DIPiDIP-5136N.
IntActiP33333. 40 interactions.
MINTiMINT-519109.

Chemistry

SwissLipidsiSLP:000000049.

Proteomic databases

MaxQBiP33333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL052C; YDL052C; YDL052C.
GeneIDi851508.
KEGGisce:YDL052C.

Organism-specific databases

EuPathDBiFungiDB:YDL052C.
SGDiS000002210. SLC1.

Phylogenomic databases

GeneTreeiENSGT00390000008726.
HOGENOMiHOG000026375.
InParanoidiP33333.
KOiK13509.
OMAiPDYKSGI.
OrthoDBiEOG092C4MB5.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciMetaCyc:YDL052C-MONOMER.
YEAST:YDL052C-MONOMER.
BRENDAi2.3.1.51. 984.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-163765. ChREBP activates metabolic gene expression.
R-SCE-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiP33333.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLSC_YEAST
AccessioniPrimary (citable) accession number: P33333
Secondary accession number(s): D6VRU4, E9P8Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.