Exocyst complex component SEC3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P33332 (SEC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Exocyst complex component SEC3

Alternative name(s):
Protein PSL1

Gene names

Name:	SEC3
Synonyms:	PSL1
Ordered Locus Names:	YER008C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Subunit structure	The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.
Miscellaneous	Present with 377 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SEC3 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane transport Inferred from mutant phenotype PubMed 7026045. Source: SGD endoplasmic reticulum inheritance Inferred from mutant phenotype PubMed 12960429 PubMed 15583030. Source: SGD exocyst assembly Inferred from mutant phenotype PubMed 15583030. Source: SGD exocyst localization Inferred from genetic interaction PubMed 17717527 PubMed 18946089. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle tethering involved in exocytosis Inferred by curator Ref.5. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 11595741 PubMed 22842922 PubMed 9491896. Source: SGD cellular bud tip Inferred from direct assay PubMed 11595741 PubMed 17717527 PubMed 22842922 PubMed 9491896. Source: SGD exocyst Inferred from direct assay Ref.5. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 11595741 PubMed 9491896. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807 PubMed 9491896. Source: SGD
Molecular_function	GTP-Rho binding Inferred from direct assay PubMed 11283608. Source: SGD phosphatidylinositol-4,5-bisphosphate binding Inferred from direct assay PubMed 18195105. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1336

1336

Exocyst complex component SEC3

PRO_0000118917

Regions

Coiled coil

319 – 464

146

Potential

Coiled coil

1309 – 1336

Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.6

Modified residue

Phosphoserine Ref.6

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

290

Phosphothreonine Ref.8

Modified residue

317

Phosphoserine Ref.11

Modified residue

318

Phosphoserine Ref.10 Ref.11

Modified residue

320

Phosphoserine Ref.11

Modified residue

321

Phosphothreonine Ref.11

Modified residue

464

Phosphoserine Ref.10 Ref.11

Modified residue

465

Phosphotyrosine Ref.11

Modified residue

548

Phosphoserine Ref.11

Modified residue

606

Phosphoserine Ref.11

Secondary structure

1336

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P33332 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 6F0C1BF13A77985E
Show »

FASTA

1,336

154,695

        10         20         30         40         50         60 
MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS APPVSGGNYS 

        70         80         90        100        110        120 
HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK TGEPPNNYIT HVRIIEDSKF 

       130        140        150        160        170        180 
PSSRPPPDSK LENKKKRLLI LSAKPNNAKL IQIHKARENS DGSFQIGRTW QLTELVRVEK 

       190        200        210        220        230        240 
DLEISEGFIL TMSKKYYWET NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE 

       250        260        270        280        290        300 
RSYQRAVITN RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA 

       310        320        330        340        350        360 
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR LQLQKENEMK 

       370        380        390        400        410        420 
RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK QMELKRQRQF EEEQRLKKER 

       430        440        450        460        470        480 
ELLEIQRKQR EQETAERLKK EEQEALAKKE EEEKSKRNKV DNESYTQEIN GKVDNLLEDL 

       490        500        510        520        530        540 
NAVLAEETET TPTMQNGTYV PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA 

       550        560        570        580        590        600 
GLNTSNLSGE DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP 

       610        620        630        640        650        660 
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID LRLAETEYLF 

       670        680        690        700        710        720 
NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS NFSNDIENVE SQDNGLQVES 

       730        740        750        760        770        780 
ANKKLLWNTL DELLKTVSLD EISLNQLLEC PIREKNLPWM ENQLNLLLKA FQAIGSDGNE 

       790        800        810        820        830        840 
VEYNLREISG LKQRLQFYEK VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF 

       850        860        870        880        890        900 
SPLILFCKEI SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL 

       910        920        930        940        950        960 
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI SSKHNFEEYI 

       970        980        990       1000       1010       1020 
KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI VTRLSSYFVE LVKAEPTVAP 

      1030       1040       1050       1060       1070       1080 
ALTFYLENEI KSLESSNHEF LLSAVTRMYT QIKQVWSDNV EEQVLHFERI SNATTNGEIL 

      1090       1100       1110       1120       1130       1140 
PGILDLPVGL KNSEDLFQFA KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN 

      1150       1160       1170       1180       1190       1200 
SSINPNLSDT AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK 

      1210       1220       1230       1240       1250       1260 
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN ILLAYTSHEI 

      1270       1280       1290       1300       1310       1320 
ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV SLYLKLYTVI DKHYRGTNIR 

      1330 
FTKNDIISAF EEYKNA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection." Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S. Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae." Finger F.P., Novick P. Mol. Biol. Cell 8:647-662(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae." TerBush D.R., Maurice T., Roth D., Novick P. EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 793-800.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32 AND SER-35, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-290, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-18, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-318 AND SER-464, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-69; SER-72; THR-74; SER-75; SER-317; SER-318; SER-320; THR-321; SER-464; TYR-465; SER-548 AND SER-606, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L22204 Genomic DNA. Translation: AAB49380.1.
U18778 Genomic DNA. Translation: AAB64541.1.
BK006939 Genomic DNA. Translation: DAA07659.1.

PIR

S41794.

RefSeq

NP_010924.1. NM_001178899.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3A58	X-ray	2.60	A/C/E	1-320	[»]
3HIE	X-ray	2.00	A/B/C/D	71-241	[»]

ProteinModelPortal

P33332.

SMR

P33332. Positions 76-260.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5992N.

IntAct

P33332. 14 interactions.

MINT

MINT-629249.

STRING

4932.YER008C.

Proteomic databases

PaxDb

P33332.

PRIDE

P33332.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER008C; YER008C; YER008C.

GeneID

856726.

KEGG

sce:YER008C.

Organism-specific databases

CYGD

YER008c.

SGD

S000000810. SEC3.

Phylogenomic databases

eggNOG

NOG81454.

GeneTree

ENSGT00660000095160.

HOGENOM

HOG000142343.

OrthoDB

EOG4SBJ66.

Gene expression databases

Genevestigator

P33332.

GermOnline

YER008C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR019160. Exocyst_Exoc1.
[Graphical view]

Pfam

PF09763. Sec3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P33332.

NextBio

982829.

Entry information

Entry name

SEC3_YEAST

Accession

Primary (citable) accession number: P33332
Secondary accession number(s): D3DLQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents