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P33332 (SEC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exocyst complex component SEC3
Alternative name(s):
Protein PSL1
Gene names
Name:SEC3
Synonyms:PSL1
Ordered Locus Names:YER008C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

Subunit structure

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SEC3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13361336Exocyst complex component SEC3
PRO_0000118917

Regions

Coiled coil319 – 464146 Potential
Coiled coil1309 – 133628 Potential

Amino acid modifications

Modified residue2901Phosphothreonine Ref.7
Modified residue6061Phosphoserine Ref.9

Secondary structure

................................ 1336
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33332 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 6F0C1BF13A77985E

FASTA1,336154,695
        10         20         30         40         50         60 
MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS APPVSGGNYS 

        70         80         90        100        110        120 
HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK TGEPPNNYIT HVRIIEDSKF 

       130        140        150        160        170        180 
PSSRPPPDSK LENKKKRLLI LSAKPNNAKL IQIHKARENS DGSFQIGRTW QLTELVRVEK 

       190        200        210        220        230        240 
DLEISEGFIL TMSKKYYWET NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE 

       250        260        270        280        290        300 
RSYQRAVITN RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA 

       310        320        330        340        350        360 
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR LQLQKENEMK 

       370        380        390        400        410        420 
RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK QMELKRQRQF EEEQRLKKER 

       430        440        450        460        470        480 
ELLEIQRKQR EQETAERLKK EEQEALAKKE EEEKSKRNKV DNESYTQEIN GKVDNLLEDL 

       490        500        510        520        530        540 
NAVLAEETET TPTMQNGTYV PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA 

       550        560        570        580        590        600 
GLNTSNLSGE DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP 

       610        620        630        640        650        660 
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID LRLAETEYLF 

       670        680        690        700        710        720 
NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS NFSNDIENVE SQDNGLQVES 

       730        740        750        760        770        780 
ANKKLLWNTL DELLKTVSLD EISLNQLLEC PIREKNLPWM ENQLNLLLKA FQAIGSDGNE 

       790        800        810        820        830        840 
VEYNLREISG LKQRLQFYEK VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF 

       850        860        870        880        890        900 
SPLILFCKEI SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL 

       910        920        930        940        950        960 
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI SSKHNFEEYI 

       970        980        990       1000       1010       1020 
KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI VTRLSSYFVE LVKAEPTVAP 

      1030       1040       1050       1060       1070       1080 
ALTFYLENEI KSLESSNHEF LLSAVTRMYT QIKQVWSDNV EEQVLHFERI SNATTNGEIL 

      1090       1100       1110       1120       1130       1140 
PGILDLPVGL KNSEDLFQFA KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN 

      1150       1160       1170       1180       1190       1200 
SSINPNLSDT AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK 

      1210       1220       1230       1240       1250       1260 
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN ILLAYTSHEI 

      1270       1280       1290       1300       1310       1320 
ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV SLYLKLYTVI DKHYRGTNIR 

      1330 
FTKNDIISAF EEYKNA 

« Hide

References

« Hide 'large scale' references
[1]"SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection."
Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.
Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae."
Finger F.P., Novick P.
Mol. Biol. Cell 8:647-662(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
TerBush D.R., Maurice T., Roth D., Novick P.
EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 793-800.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-606, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22204 Genomic DNA. Translation: AAB49380.1.
U18778 Genomic DNA. Translation: AAB64541.1.
BK006939 Genomic DNA. Translation: DAA07659.1.
PIRS41794.
RefSeqNP_010924.1. NM_001178899.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3A58X-ray2.60A/C/E1-320[»]
3HIEX-ray2.00A/B/C/D71-241[»]
ProteinModelPortalP33332.
SMRP33332. Positions 76-260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36739. 114 interactions.
DIPDIP-5992N.
IntActP33332. 16 interactions.
MINTMINT-629249.
STRING4932.YER008C.

Proteomic databases

MaxQBP33332.
PaxDbP33332.
PRIDEP33332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER008C; YER008C; YER008C.
GeneID856726.
KEGGsce:YER008C.

Organism-specific databases

CYGDYER008c.
SGDS000000810. SEC3.

Phylogenomic databases

eggNOGNOG81454.
HOGENOMHOG000142343.
OMAFKILAPE.
OrthoDBEOG7SFJ59.

Enzyme and pathway databases

BioCycYEAST:G3O-30195-MONOMER.

Gene expression databases

GenevestigatorP33332.

Family and domain databases

InterProIPR019160. Exocyst_Exoc1/SEC3.
IPR028258. Sec3-PIP2_bind.
[Graphical view]
PfamPF15277. Sec3-PIP2_bind. 1 hit.
PF09763. Sec3_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33332.
NextBio982829.

Entry information

Entry nameSEC3_YEAST
AccessionPrimary (citable) accession number: P33332
Secondary accession number(s): D3DLQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references