SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P33332

- SEC3_YEAST

UniProt

P33332 - SEC3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Exocyst complex component SEC3
Gene
SEC3, PSL1, YER008C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

GO - Molecular functioni

  1. GTP-Rho binding Source: SGD
  2. phosphatidylinositol-4,5-bisphosphate binding Source: SGD
Complete GO annotation...

GO - Biological processi

  1. Golgi to plasma membrane transport Source: SGD
  2. endoplasmic reticulum inheritance Source: SGD
  3. exocyst assembly Source: SGD
  4. exocyst localization Source: SGD
  5. exocytosis Source: SGD
  6. protein transport Source: UniProtKB-KW
  7. vesicle tethering involved in exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30195-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component SEC3
Alternative name(s):
Protein PSL1
Gene namesi
Name:SEC3
Synonyms:PSL1
Ordered Locus Names:YER008C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER008c.
SGDiS000000810. SEC3.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. exocyst Source: SGD
  4. incipient cellular bud site Source: SGD
  5. mating projection tip Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13361336Exocyst complex component SEC3
PRO_0000118917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei290 – 2901Phosphothreonine1 Publication
Modified residuei606 – 6061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33332.
PaxDbiP33332.
PRIDEiP33332.

Expressioni

Gene expression databases

GenevestigatoriP33332.

Interactioni

Subunit structurei

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.

Protein-protein interaction databases

BioGridi36739. 114 interactions.
DIPiDIP-5992N.
IntActiP33332. 16 interactions.
MINTiMINT-629249.
STRINGi4932.YER008C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi76 – 9318
Beta strandi94 – 963
Beta strandi99 – 1024
Beta strandi107 – 12317
Helixi131 – 1333
Beta strandi134 – 14310
Beta strandi151 – 1588
Helixi160 – 1623
Beta strandi164 – 1718
Helixi172 – 1743
Beta strandi177 – 1804
Beta strandi187 – 19913
Helixi203 – 21917
Turni221 – 2233
Beta strandi229 – 2313
Beta strandi235 – 2384
Helixi240 – 2467

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A58X-ray2.60A/C/E1-320[»]
3HIEX-ray2.00A/B/C/D71-241[»]
ProteinModelPortaliP33332.
SMRiP33332. Positions 76-260.

Miscellaneous databases

EvolutionaryTraceiP33332.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili319 – 464146 Reviewed prediction
Add
BLAST
Coiled coili1309 – 133628 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the SEC3 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG81454.
HOGENOMiHOG000142343.
OMAiFKILAPE.
OrthoDBiEOG7SFJ59.

Family and domain databases

InterProiIPR019160. Exocyst_Exoc1/SEC3.
IPR028258. Sec3-PIP2_bind.
[Graphical view]
PfamiPF15277. Sec3-PIP2_bind. 1 hit.
PF09763. Sec3_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33332-1 [UniParc]FASTAAdd to Basket

« Hide

MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS     50
APPVSGGNYS HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK 100
TGEPPNNYIT HVRIIEDSKF PSSRPPPDSK LENKKKRLLI LSAKPNNAKL 150
IQIHKARENS DGSFQIGRTW QLTELVRVEK DLEISEGFIL TMSKKYYWET 200
NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE RSYQRAVITN 250
RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA 300
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR 350
LQLQKENEMK RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK 400
QMELKRQRQF EEEQRLKKER ELLEIQRKQR EQETAERLKK EEQEALAKKE 450
EEEKSKRNKV DNESYTQEIN GKVDNLLEDL NAVLAEETET TPTMQNGTYV 500
PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA GLNTSNLSGE 550
DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP 600
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID 650
LRLAETEYLF NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS 700
NFSNDIENVE SQDNGLQVES ANKKLLWNTL DELLKTVSLD EISLNQLLEC 750
PIREKNLPWM ENQLNLLLKA FQAIGSDGNE VEYNLREISG LKQRLQFYEK 800
VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF SPLILFCKEI 850
SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL 900
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI 950
SSKHNFEEYI KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI 1000
VTRLSSYFVE LVKAEPTVAP ALTFYLENEI KSLESSNHEF LLSAVTRMYT 1050
QIKQVWSDNV EEQVLHFERI SNATTNGEIL PGILDLPVGL KNSEDLFQFA 1100
KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN SSINPNLSDT 1150
AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK 1200
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN 1250
ILLAYTSHEI ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV 1300
SLYLKLYTVI DKHYRGTNIR FTKNDIISAF EEYKNA 1336
Length:1,336
Mass (Da):154,695
Last modified:February 1, 1994 - v1
Checksum:i6F0C1BF13A77985E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22204 Genomic DNA. Translation: AAB49380.1.
U18778 Genomic DNA. Translation: AAB64541.1.
BK006939 Genomic DNA. Translation: DAA07659.1.
PIRiS41794.
RefSeqiNP_010924.1. NM_001178899.1.

Genome annotation databases

EnsemblFungiiYER008C; YER008C; YER008C.
GeneIDi856726.
KEGGisce:YER008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22204 Genomic DNA. Translation: AAB49380.1 .
U18778 Genomic DNA. Translation: AAB64541.1 .
BK006939 Genomic DNA. Translation: DAA07659.1 .
PIRi S41794.
RefSeqi NP_010924.1. NM_001178899.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3A58 X-ray 2.60 A/C/E 1-320 [» ]
3HIE X-ray 2.00 A/B/C/D 71-241 [» ]
ProteinModelPortali P33332.
SMRi P33332. Positions 76-260.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36739. 114 interactions.
DIPi DIP-5992N.
IntActi P33332. 16 interactions.
MINTi MINT-629249.
STRINGi 4932.YER008C.

Proteomic databases

MaxQBi P33332.
PaxDbi P33332.
PRIDEi P33332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER008C ; YER008C ; YER008C .
GeneIDi 856726.
KEGGi sce:YER008C.

Organism-specific databases

CYGDi YER008c.
SGDi S000000810. SEC3.

Phylogenomic databases

eggNOGi NOG81454.
HOGENOMi HOG000142343.
OMAi FKILAPE.
OrthoDBi EOG7SFJ59.

Enzyme and pathway databases

BioCyci YEAST:G3O-30195-MONOMER.

Miscellaneous databases

EvolutionaryTracei P33332.
NextBioi 982829.

Gene expression databases

Genevestigatori P33332.

Family and domain databases

InterProi IPR019160. Exocyst_Exoc1/SEC3.
IPR028258. Sec3-PIP2_bind.
[Graphical view ]
Pfami PF15277. Sec3-PIP2_bind. 1 hit.
PF09763. Sec3_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection."
    Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.
    Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae."
    Finger F.P., Novick P.
    Mol. Biol. Cell 8:647-662(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
    TerBush D.R., Maurice T., Roth D., Novick P.
    EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 793-800.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-606, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSEC3_YEAST
AccessioniPrimary (citable) accession number: P33332
Secondary accession number(s): D3DLQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi