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Protein

Exocyst complex component SEC3

Gene

SEC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

GO - Molecular functioni

  • GTP-Rho binding Source: SGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: SGD

GO - Biological processi

  • endoplasmic reticulum inheritance Source: SGD
  • exocyst assembly Source: SGD
  • exocyst localization Source: SGD
  • exocytosis Source: SGD
  • Golgi to plasma membrane transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle tethering involved in exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30195-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component SEC3
Alternative name(s):
Protein PSL1
Gene namesi
Name:SEC3
Synonyms:PSL1
Ordered Locus Names:YER008C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER008C.
SGDiS000000810. SEC3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • exocyst Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • plasma membrane Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001189171 – 1336Exocyst complex component SEC3Add BLAST1336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei290PhosphothreonineCombined sources1
Modified residuei606PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33332.
PRIDEiP33332.

PTM databases

iPTMnetiP33332.

Interactioni

Subunit structurei

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.

GO - Molecular functioni

  • GTP-Rho binding Source: SGD

Protein-protein interaction databases

BioGridi36739. 119 interactors.
DIPiDIP-5992N.
IntActiP33332. 17 interactors.
MINTiMINT-629249.

Structurei

Secondary structure

11336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi76 – 93Combined sources18
Beta strandi94 – 96Combined sources3
Beta strandi99 – 102Combined sources4
Beta strandi107 – 123Combined sources17
Helixi131 – 133Combined sources3
Beta strandi134 – 143Combined sources10
Beta strandi151 – 158Combined sources8
Helixi160 – 162Combined sources3
Beta strandi164 – 171Combined sources8
Helixi172 – 174Combined sources3
Beta strandi177 – 180Combined sources4
Beta strandi187 – 199Combined sources13
Helixi203 – 219Combined sources17
Turni221 – 223Combined sources3
Beta strandi229 – 231Combined sources3
Beta strandi235 – 238Combined sources4
Helixi240 – 246Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A58X-ray2.60A/C/E1-320[»]
3HIEX-ray2.00A/B/C/D71-241[»]
ProteinModelPortaliP33332.
SMRiP33332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33332.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili319 – 464Sequence analysisAdd BLAST146
Coiled coili1309 – 1336Sequence analysisAdd BLAST28

Sequence similaritiesi

Belongs to the SEC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000142343.
InParanoidiP33332.
KOiK19983.
OMAiNNYITHV.
OrthoDBiEOG092C05LD.

Family and domain databases

InterProiIPR028258. Sec3-PIP2_bind.
IPR019160. Sec3_C.
[Graphical view]
PfamiPF15277. Sec3-PIP2_bind. 1 hit.
PF09763. Sec3_C. 1 hit.
[Graphical view]
SMARTiSM01313. Sec3-PIP2_bind. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS
60 70 80 90 100
APPVSGGNYS HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK
110 120 130 140 150
TGEPPNNYIT HVRIIEDSKF PSSRPPPDSK LENKKKRLLI LSAKPNNAKL
160 170 180 190 200
IQIHKARENS DGSFQIGRTW QLTELVRVEK DLEISEGFIL TMSKKYYWET
210 220 230 240 250
NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE RSYQRAVITN
260 270 280 290 300
RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA
310 320 330 340 350
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR
360 370 380 390 400
LQLQKENEMK RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK
410 420 430 440 450
QMELKRQRQF EEEQRLKKER ELLEIQRKQR EQETAERLKK EEQEALAKKE
460 470 480 490 500
EEEKSKRNKV DNESYTQEIN GKVDNLLEDL NAVLAEETET TPTMQNGTYV
510 520 530 540 550
PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA GLNTSNLSGE
560 570 580 590 600
DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP
610 620 630 640 650
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID
660 670 680 690 700
LRLAETEYLF NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS
710 720 730 740 750
NFSNDIENVE SQDNGLQVES ANKKLLWNTL DELLKTVSLD EISLNQLLEC
760 770 780 790 800
PIREKNLPWM ENQLNLLLKA FQAIGSDGNE VEYNLREISG LKQRLQFYEK
810 820 830 840 850
VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF SPLILFCKEI
860 870 880 890 900
SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL
910 920 930 940 950
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI
960 970 980 990 1000
SSKHNFEEYI KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI
1010 1020 1030 1040 1050
VTRLSSYFVE LVKAEPTVAP ALTFYLENEI KSLESSNHEF LLSAVTRMYT
1060 1070 1080 1090 1100
QIKQVWSDNV EEQVLHFERI SNATTNGEIL PGILDLPVGL KNSEDLFQFA
1110 1120 1130 1140 1150
KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN SSINPNLSDT
1160 1170 1180 1190 1200
AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK
1210 1220 1230 1240 1250
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN
1260 1270 1280 1290 1300
ILLAYTSHEI ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV
1310 1320 1330
SLYLKLYTVI DKHYRGTNIR FTKNDIISAF EEYKNA
Length:1,336
Mass (Da):154,695
Last modified:February 1, 1994 - v1
Checksum:i6F0C1BF13A77985E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22204 Genomic DNA. Translation: AAB49380.1.
U18778 Genomic DNA. Translation: AAB64541.1.
BK006939 Genomic DNA. Translation: DAA07659.1.
PIRiS41794.
RefSeqiNP_010924.1. NM_001178899.1.

Genome annotation databases

EnsemblFungiiYER008C; YER008C; YER008C.
GeneIDi856726.
KEGGisce:YER008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22204 Genomic DNA. Translation: AAB49380.1.
U18778 Genomic DNA. Translation: AAB64541.1.
BK006939 Genomic DNA. Translation: DAA07659.1.
PIRiS41794.
RefSeqiNP_010924.1. NM_001178899.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A58X-ray2.60A/C/E1-320[»]
3HIEX-ray2.00A/B/C/D71-241[»]
ProteinModelPortaliP33332.
SMRiP33332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36739. 119 interactors.
DIPiDIP-5992N.
IntActiP33332. 17 interactors.
MINTiMINT-629249.

PTM databases

iPTMnetiP33332.

Proteomic databases

MaxQBiP33332.
PRIDEiP33332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER008C; YER008C; YER008C.
GeneIDi856726.
KEGGisce:YER008C.

Organism-specific databases

EuPathDBiFungiDB:YER008C.
SGDiS000000810. SEC3.

Phylogenomic databases

HOGENOMiHOG000142343.
InParanoidiP33332.
KOiK19983.
OMAiNNYITHV.
OrthoDBiEOG092C05LD.

Enzyme and pathway databases

BioCyciYEAST:G3O-30195-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP33332.
PROiP33332.

Family and domain databases

InterProiIPR028258. Sec3-PIP2_bind.
IPR019160. Sec3_C.
[Graphical view]
PfamiPF15277. Sec3-PIP2_bind. 1 hit.
PF09763. Sec3_C. 1 hit.
[Graphical view]
SMARTiSM01313. Sec3-PIP2_bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEC3_YEAST
AccessioniPrimary (citable) accession number: P33332
Secondary accession number(s): D3DLQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.