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P33331

- NTF2_YEAST

UniProt

P33331 - NTF2_YEAST

Protein

Nuclear transport factor 2

Gene

NTF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. In vitro, the NTF2-Ran-GDP association, in the presence of GTP, triggers dissociation of the karyopherin alpha-beta complex, allowing nuclear translocation of karyopherin alpha and the NLS substrate.

    GO - Molecular functioni

    1. Ran GTPase binding Source: SGD

    GO - Biological processi

    1. nucleocytoplasmic transport Source: SGD
    2. protein import into nucleus Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30196-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear transport factor 2
    Short name:
    NTF-2
    Alternative name(s):
    Nuclear transport factor P10
    Gene namesi
    Name:NTF2
    Ordered Locus Names:YER009W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER009w.
    SGDiS000000811. NTF2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear envelope Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 125124Nuclear transport factor 2PRO_0000194792Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP33331.
    PaxDbiP33331.
    PeptideAtlasiP33331.

    Expressioni

    Gene expression databases

    GenevestigatoriP33331.

    Interactioni

    Protein-protein interaction databases

    BioGridi36740. 20 interactions.
    DIPiDIP-2245N.
    IntActiP33331. 1 interaction.
    MINTiMINT-525215.
    STRINGi4932.YER009W.

    Structurei

    Secondary structure

    1
    125
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 2319
    Helixi24 – 307
    Beta strandi31 – 399
    Beta strandi42 – 465
    Helixi47 – 5610
    Beta strandi62 – 7514
    Beta strandi80 – 9011
    Beta strandi97 – 10812
    Beta strandi111 – 12212

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GY7X-ray1.60A/B/C/D1-125[»]
    1GYBX-ray1.90A/B/C/D1-125[»]
    ProteinModelPortaliP33331.
    SMRiP33331. Positions 3-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33331.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 121114NTF2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 NTF2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG286483.
    GeneTreeiENSGT00510000047030.
    HOGENOMiHOG000197024.
    OMAiQFTEFYY.
    OrthoDBiEOG78PVP2.

    Family and domain databases

    InterProiIPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    [Graphical view]
    PfamiPF02136. NTF2. 1 hit.
    [Graphical view]
    PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33331-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLDFNTLAQ NFTQFYYNQF DTDRSQLGNL YRNESMLTFE TSQLQGAKDI    50
    VEKLVSLPFQ KVQHRITTLD AQPASPNGDV LVMITGDLLI DEEQNPQRFS 100
    QVFHLIPDGN SYYVFNDIFR LNYSA 125
    Length:125
    Mass (Da):14,453
    Last modified:February 1, 1995 - v2
    Checksum:i5660D8C00E634714
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64542.1.
    AY558448 Genomic DNA. Translation: AAS56774.1.
    L22204 Genomic DNA. Translation: AAB49379.1.
    BK006939 Genomic DNA. Translation: DAA07660.1.
    PIRiS50467.
    RefSeqiNP_010925.1. NM_001178900.1.

    Genome annotation databases

    EnsemblFungiiYER009W; YER009W; YER009W.
    GeneIDi856727.
    KEGGisce:YER009W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64542.1 .
    AY558448 Genomic DNA. Translation: AAS56774.1 .
    L22204 Genomic DNA. Translation: AAB49379.1 .
    BK006939 Genomic DNA. Translation: DAA07660.1 .
    PIRi S50467.
    RefSeqi NP_010925.1. NM_001178900.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GY7 X-ray 1.60 A/B/C/D 1-125 [» ]
    1GYB X-ray 1.90 A/B/C/D 1-125 [» ]
    ProteinModelPortali P33331.
    SMRi P33331. Positions 3-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36740. 20 interactions.
    DIPi DIP-2245N.
    IntActi P33331. 1 interaction.
    MINTi MINT-525215.
    STRINGi 4932.YER009W.

    Proteomic databases

    MaxQBi P33331.
    PaxDbi P33331.
    PeptideAtlasi P33331.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER009W ; YER009W ; YER009W .
    GeneIDi 856727.
    KEGGi sce:YER009W.

    Organism-specific databases

    CYGDi YER009w.
    SGDi S000000811. NTF2.

    Phylogenomic databases

    eggNOGi NOG286483.
    GeneTreei ENSGT00510000047030.
    HOGENOMi HOG000197024.
    OMAi QFTEFYY.
    OrthoDBi EOG78PVP2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30196-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P33331.
    NextBioi 982832.
    PROi P33331.

    Gene expression databases

    Genevestigatori P33331.

    Family and domain databases

    InterProi IPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    [Graphical view ]
    Pfami PF02136. NTF2. 1 hit.
    [Graphical view ]
    PROSITEi PS50177. NTF2_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection."
      Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.
      Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
    5. "Role of the nuclear transport factor p10 in nuclear import."
      Nehrbass U., Blobel G.
      Science 272:120-122(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats."
      Bayliss R., Leung S.W., Baker R.P., Quimby B.B., Corbett A.H., Stewart M.
      EMBO J. 21:2843-2853(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiNTF2_YEAST
    AccessioniPrimary (citable) accession number: P33331
    Secondary accession number(s): D3DLQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3