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Protein

Nuclear transport factor 2

Gene

NTF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. In vitro, the NTF2-Ran-GDP association, in the presence of GTP, triggers dissociation of the karyopherin alpha-beta complex, allowing nuclear translocation of karyopherin alpha and the NLS substrate.

GO - Molecular functioni

  1. Ran GTPase binding Source: SGD

GO - Biological processi

  1. nucleocytoplasmic transport Source: SGD
  2. protein import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30196-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transport factor 2
Short name:
NTF-2
Alternative name(s):
Nuclear transport factor P10
Gene namesi
Name:NTF2
Ordered Locus Names:YER009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER009w.
SGDiS000000811. NTF2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nuclear envelope Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 125124Nuclear transport factor 2PRO_0000194792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP33331.
PaxDbiP33331.
PeptideAtlasiP33331.

Expressioni

Gene expression databases

GenevestigatoriP33331.

Interactioni

Protein-protein interaction databases

BioGridi36740. 21 interactions.
DIPiDIP-2245N.
IntActiP33331. 1 interaction.
MINTiMINT-525215.
STRINGi4932.YER009W.

Structurei

Secondary structure

1
125
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2319Combined sources
Helixi24 – 307Combined sources
Beta strandi31 – 399Combined sources
Beta strandi42 – 465Combined sources
Helixi47 – 5610Combined sources
Beta strandi62 – 7514Combined sources
Beta strandi80 – 9011Combined sources
Beta strandi97 – 10812Combined sources
Beta strandi111 – 12212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GY7X-ray1.60A/B/C/D1-125[»]
1GYBX-ray1.90A/B/C/D1-125[»]
ProteinModelPortaliP33331.
SMRiP33331. Positions 3-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33331.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 121114NTF2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286483.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
InParanoidiP33331.
OMAiQFTEFYY.
OrthoDBiEOG78PVP2.

Family and domain databases

InterProiIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33331-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLDFNTLAQ NFTQFYYNQF DTDRSQLGNL YRNESMLTFE TSQLQGAKDI
60 70 80 90 100
VEKLVSLPFQ KVQHRITTLD AQPASPNGDV LVMITGDLLI DEEQNPQRFS
110 120
QVFHLIPDGN SYYVFNDIFR LNYSA
Length:125
Mass (Da):14,453
Last modified:February 1, 1995 - v2
Checksum:i5660D8C00E634714
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64542.1.
AY558448 Genomic DNA. Translation: AAS56774.1.
L22204 Genomic DNA. Translation: AAB49379.1.
BK006939 Genomic DNA. Translation: DAA07660.1.
PIRiS50467.
RefSeqiNP_010925.1. NM_001178900.1.

Genome annotation databases

EnsemblFungiiYER009W; YER009W; YER009W.
GeneIDi856727.
KEGGisce:YER009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64542.1.
AY558448 Genomic DNA. Translation: AAS56774.1.
L22204 Genomic DNA. Translation: AAB49379.1.
BK006939 Genomic DNA. Translation: DAA07660.1.
PIRiS50467.
RefSeqiNP_010925.1. NM_001178900.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GY7X-ray1.60A/B/C/D1-125[»]
1GYBX-ray1.90A/B/C/D1-125[»]
ProteinModelPortaliP33331.
SMRiP33331. Positions 3-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36740. 21 interactions.
DIPiDIP-2245N.
IntActiP33331. 1 interaction.
MINTiMINT-525215.
STRINGi4932.YER009W.

Proteomic databases

MaxQBiP33331.
PaxDbiP33331.
PeptideAtlasiP33331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER009W; YER009W; YER009W.
GeneIDi856727.
KEGGisce:YER009W.

Organism-specific databases

CYGDiYER009w.
SGDiS000000811. NTF2.

Phylogenomic databases

eggNOGiNOG286483.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
InParanoidiP33331.
OMAiQFTEFYY.
OrthoDBiEOG78PVP2.

Enzyme and pathway databases

BioCyciYEAST:G3O-30196-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP33331.
NextBioi982832.
PROiP33331.

Gene expression databases

GenevestigatoriP33331.

Family and domain databases

InterProiIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection."
    Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.
    Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
  5. "Role of the nuclear transport factor p10 in nuclear import."
    Nehrbass U., Blobel G.
    Science 272:120-122(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats."
    Bayliss R., Leung S.W., Baker R.P., Quimby B.B., Corbett A.H., Stewart M.
    EMBO J. 21:2843-2853(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiNTF2_YEAST
AccessioniPrimary (citable) accession number: P33331
Secondary accession number(s): D3DLQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.