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P33331 (NTF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear transport factor 2

Short name=NTF-2
Alternative name(s):
Nuclear transport factor P10
Gene names
Name:NTF2
Ordered Locus Names:YER009W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length125 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. In vitro, the NTF2-Ran-GDP association, in the presence of GTP, triggers dissociation of the karyopherin alpha-beta complex, allowing nuclear translocation of karyopherin alpha and the NLS substrate.

Subcellular location

Cytoplasm.

Sequence similarities

Contains 1 NTF2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 125124Nuclear transport factor 2
PRO_0000194792

Regions

Domain8 – 121114NTF2

Amino acid modifications

Modified residue21N-acetylserine Ref.6

Secondary structure

................ 125
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33331 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 5660D8C00E634714

FASTA12514,453
        10         20         30         40         50         60 
MSLDFNTLAQ NFTQFYYNQF DTDRSQLGNL YRNESMLTFE TSQLQGAKDI VEKLVSLPFQ 

        70         80         90        100        110        120 
KVQHRITTLD AQPASPNGDV LVMITGDLLI DEEQNPQRFS QVFHLIPDGN SYYVFNDIFR 


LNYSA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection."
Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.
Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
[5]"Role of the nuclear transport factor p10 in nuclear import."
Nehrbass U., Blobel G.
Science 272:120-122(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[7]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats."
Bayliss R., Leung S.W., Baker R.P., Quimby B.B., Corbett A.H., Stewart M.
EMBO J. 21:2843-2853(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18778 Genomic DNA. Translation: AAB64542.1.
AY558448 Genomic DNA. Translation: AAS56774.1.
L22204 Genomic DNA. Translation: AAB49379.1.
BK006939 Genomic DNA. Translation: DAA07660.1.
PIRS50467.
RefSeqNP_010925.1. NM_001178900.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GY7X-ray1.60A/B/C/D1-125[»]
1GYBX-ray1.90A/B/C/D1-125[»]
ProteinModelPortalP33331.
SMRP33331. Positions 3-124.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36740. 20 interactions.
DIPDIP-2245N.
IntActP33331. 1 interaction.
MINTMINT-525215.
STRING4932.YER009W.

Proteomic databases

PaxDbP33331.
PeptideAtlasP33331.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER009W; YER009W; YER009W.
GeneID856727.
KEGGsce:YER009W.

Organism-specific databases

CYGDYER009w.
SGDS000000811. NTF2.

Phylogenomic databases

eggNOGNOG286483.
GeneTreeENSGT00510000047030.
HOGENOMHOG000197024.
OMAQFTEFYY.
OrthoDBEOG78PVP2.

Enzyme and pathway databases

BioCycYEAST:G3O-30196-MONOMER.

Gene expression databases

GenevestigatorP33331.

Family and domain databases

InterProIPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamPF02136. NTF2. 1 hit.
[Graphical view]
PROSITEPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33331.
NextBio982832.
PROP33331.

Entry information

Entry nameNTF2_YEAST
AccessionPrimary (citable) accession number: P33331
Secondary accession number(s): D3DLQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references