Nuclear transport factor 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P33331 (NTF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear transport factor 2
Short name=NTF-2

Alternative name(s):
Nuclear transport factor P10

Gene names

Name:	NTF2
Ordered Locus Names:	YER009W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. In vitro, the NTF2-Ran-GDP association, in the presence of GTP, triggers dissociation of the karyopherin alpha-beta complex, allowing nuclear translocation of karyopherin alpha and the NLS substrate.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 NTF2 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into nucleus Inferred from mutant phenotype PubMed 8702493. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD nuclear envelope Inferred from direct assay PubMed 8702493. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 125

125

Nuclear transport factor 2

PRO_0000194792

Regions

Domain

8 – 121

114

NTF2

Secondary structure

125

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P33331 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 5660D8C00E634714
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FASTA

125

14,453

        10         20         30         40         50         60 
MSLDFNTLAQ NFTQFYYNQF DTDRSQLGNL YRNESMLTFE TSQLQGAKDI VEKLVSLPFQ 

        70         80         90        100        110        120 
KVQHRITTLD AQPASPNGDV LVMITGDLLI DEEQNPQRFS QVFHLIPDGN SYYVFNDIFR 


LNYSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection." Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S. Genetics 144:495-510(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
[5]	"Role of the nuclear transport factor p10 in nuclear import." Nehrbass U., Blobel G. Science 272:120-122(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats." Bayliss R., Leung S.W., Baker R.P., Quimby B.B., Corbett A.H., Stewart M. EMBO J. 21:2843-2853(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18778 Genomic DNA. Translation: AAB64542.1.
AY558448 Genomic DNA. Translation: AAS56774.1.
L22204 Genomic DNA. Translation: AAB49379.1.
BK006939 Genomic DNA. Translation: DAA07660.1.

PIR

S50467.

RefSeq

NP_010925.1. NM_001178900.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GY7	X-ray	1.60	A/B/C/D	1-125	[»]
1GYB	X-ray	1.90	A/B/C/D	1-125	[»]

ProteinModelPortal

P33331.

SMR

P33331. Positions 3-124.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2245N.

IntAct

P33331. 2 interactions.

MINT

MINT-525215.

STRING

4932.YER009W.

Proteomic databases

PaxDb

P33331.

PeptideAtlas

P33331.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER009W; YER009W; YER009W.

GeneID

856727.

KEGG

sce:YER009W.

Organism-specific databases

CYGD

YER009w.

SGD

S000000811. NTF2.

Phylogenomic databases

eggNOG

NOG286483.

GeneTree

ENSGT00510000047030.

HOGENOM

HOG000197024.

OMA

MTFEGVQ.

OrthoDB

EOG4HHSBX.

Gene expression databases

Genevestigator

P33331.

GermOnline

YER009W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]

Pfam

PF02136. NTF2. 1 hit.
[Graphical view]

PROSITE

PS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P33331.

NextBio

982832.

Entry information

Entry name

NTF2_YEAST

Accession

Primary (citable) accession number: P33331
Secondary accession number(s): D3DLQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents