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Protein

Phosphoserine aminotransferase

Gene

SER1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.By similarity

Catalytic activityi

O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.

Cofactori

pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity

Pathwayi: L-serine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase 1 (SER3), D-3-phosphoglycerate dehydrogenase 2 (SER33)
  2. Phosphoserine aminotransferase (SER1)
  3. Phosphoserine phosphatase (SER2)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Phosphoserine aminotransferase (SER1)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461L-glutamateBy similarity
Binding sitei113 – 1131Pyridoxal phosphateBy similarity
Binding sitei170 – 1701Pyridoxal phosphateBy similarity
Binding sitei194 – 1941Pyridoxal phosphateBy similarity
Binding sitei217 – 2171Pyridoxal phosphateBy similarity

GO - Molecular functioni

  • O-phospho-L-serine:2-oxoglutarate aminotransferase activity Source: SGD

GO - Biological processi

  • L-serine biosynthetic process Source: SGD
  • purine nucleobase biosynthetic process Source: SGD
  • serine family amino acid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Serine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YOR184W-MONOMER.
ReactomeiR-SCE-977347. Serine biosynthesis.
UniPathwayiUPA00135; UER00197.
UPA00244; UER00311.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine aminotransferase (EC:2.6.1.52)
Short name:
PSAT
Alternative name(s):
Phosphohydroxythreonine aminotransferase
Gene namesi
Name:SER1
Synonyms:SERC
Ordered Locus Names:YOR184W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR184W.
SGDiS000005710. SER1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Phosphoserine aminotransferasePRO_0000150141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201PhosphothreonineCombined sources
Modified residuei112 – 1121PhosphoserineCombined sources
Modified residuei218 – 2181N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33330.

2D gel databases

SWISS-2DPAGEP33330.

PTM databases

iPTMnetiP33330.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi34579. 101 interactions.
DIPiDIP-4686N.
IntActiP33330. 6 interactions.
MINTiMINT-496928.

Structurei

3D structure databases

ProteinModelPortaliP33330.
SMRiP33330. Positions 10-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 812Pyridoxal phosphate bindingBy similarity
Regioni271 – 2722Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000015177.
HOGENOMiHOG000088965.
InParanoidiP33330.
KOiK00831.
OMAiDTHEVFF.
OrthoDBiEOG092C2RHE.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00160. SerC_aminotrans_5. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR022278. Pser_aminoTfrase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000525. SerC. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01364. serC_1. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEREEPQH FGAGPAQMPT PVLQQAAKDL INFNDIGLGI GEISHRSKDA
60 70 80 90 100
TKVIEDSKKH LIELLNIPDT HEVFYLQGGG TTGFSSVATN LAAAYVGKHG
110 120 130 140 150
KIAPAGYLVT GSWSQKSFEE AKRLHVPAEV IFNAKDYNNG KFGKIPDESL
160 170 180 190 200
WEDKIKGKAF SYVYLCENET VHGVEWPELP KCLVNDPNIE IVADLSSDIL
210 220 230 240 250
SRKIDVSQYG VIMAGAQKNI GLAGLTLYII KKSILKNISG ASDETLHELG
260 270 280 290 300
VPITPIAFDY PTVVKNNSAY NTIPIFTLHV MDLVFQHILK KGGVEAQQAE
310 320 330 340 350
NEEKAKILYE ALDANSDFYN VPVDPKCRSK MNVVFTLKKD GLDDQFLKEA
360 370 380 390
AARHLTGLKG HRSVGGFRAS IYNALSVKAV QNLVDFIKEF AEKNA
Length:395
Mass (Da):43,416
Last modified:February 1, 1994 - v1
Checksum:iA22F020CC969B4BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti258 – 2592FD → LH in AAA85703 (PubMed:7553933).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20917 Genomic DNA. Translation: AAA20886.1.
U19714 Genomic DNA. Translation: AAA85703.1.
Z75092 Genomic DNA. Translation: CAA99393.1.
BK006948 Genomic DNA. Translation: DAA10956.1.
PIRiS42680.
RefSeqiNP_014827.3. NM_001183603.3.

Genome annotation databases

EnsemblFungiiYOR184W; YOR184W; YOR184W.
GeneIDi854356.
KEGGisce:YOR184W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20917 Genomic DNA. Translation: AAA20886.1.
U19714 Genomic DNA. Translation: AAA85703.1.
Z75092 Genomic DNA. Translation: CAA99393.1.
BK006948 Genomic DNA. Translation: DAA10956.1.
PIRiS42680.
RefSeqiNP_014827.3. NM_001183603.3.

3D structure databases

ProteinModelPortaliP33330.
SMRiP33330. Positions 10-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34579. 101 interactions.
DIPiDIP-4686N.
IntActiP33330. 6 interactions.
MINTiMINT-496928.

PTM databases

iPTMnetiP33330.

2D gel databases

SWISS-2DPAGEP33330.

Proteomic databases

MaxQBiP33330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR184W; YOR184W; YOR184W.
GeneIDi854356.
KEGGisce:YOR184W.

Organism-specific databases

EuPathDBiFungiDB:YOR184W.
SGDiS000005710. SER1.

Phylogenomic databases

GeneTreeiENSGT00390000015177.
HOGENOMiHOG000088965.
InParanoidiP33330.
KOiK00831.
OMAiDTHEVFF.
OrthoDBiEOG092C2RHE.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00197.
UPA00244; UER00311.
BioCyciYEAST:YOR184W-MONOMER.
ReactomeiR-SCE-977347. Serine biosynthesis.

Miscellaneous databases

PROiP33330.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00160. SerC_aminotrans_5. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR022278. Pser_aminoTfrase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000525. SerC. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01364. serC_1. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERC_YEAST
AccessioniPrimary (citable) accession number: P33330
Secondary accession number(s): D6W2P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.