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Protein

Phosphoserine aminotransferase

Gene

SER1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.By similarity

Miscellaneous

Present with 15900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.

Cofactori

pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity

Pathwayi: L-serine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase 1 (SER3), D-3-phosphoglycerate dehydrogenase 2 (SER33)
  2. Phosphoserine aminotransferase (SER1)
  3. Phosphoserine phosphatase (SER2)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Phosphoserine aminotransferase (SER1)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46L-glutamateBy similarity1
Binding sitei113Pyridoxal phosphateBy similarity1
Binding sitei170Pyridoxal phosphateBy similarity1
Binding sitei194Pyridoxal phosphateBy similarity1
Binding sitei217Pyridoxal phosphateBy similarity1

GO - Molecular functioni

  • O-phospho-L-serine:2-oxoglutarate aminotransferase activity Source: SGD

GO - Biological processi

  • L-serine biosynthetic process Source: SGD
  • purine nucleobase biosynthetic process Source: SGD
  • serine family amino acid biosynthetic process Source: SGD

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Serine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YOR184W-MONOMER
ReactomeiR-SCE-977347 Serine biosynthesis
UniPathwayiUPA00135; UER00197
UPA00244; UER00311

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine aminotransferase (EC:2.6.1.52)
Short name:
PSAT
Alternative name(s):
Phosphohydroxythreonine aminotransferase
Gene namesi
Name:SER1
Synonyms:SERC
Ordered Locus Names:YOR184W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR184W
SGDiS000005710 SER1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001501411 – 395Phosphoserine aminotransferaseAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphothreonineCombined sources1
Modified residuei112PhosphoserineCombined sources1
Modified residuei218N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33330
PaxDbiP33330
PRIDEiP33330

2D gel databases

SWISS-2DPAGEiP33330

PTM databases

iPTMnetiP33330

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
UBA1P2251510EBI-16970,EBI-19703

Protein-protein interaction databases

BioGridi34579, 245 interactors
DIPiDIP-4686N
IntActiP33330, 15 interactors
MINTiP33330
STRINGi4932.YOR184W

Structurei

3D structure databases

ProteinModelPortaliP33330
SMRiP33330
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 81Pyridoxal phosphate bindingBy similarity2
Regioni271 – 272Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000015177
HOGENOMiHOG000088965
InParanoidiP33330
KOiK00831
OMAiGAQKNMG
OrthoDBiEOG092C2RHE

Family and domain databases

CDDicd00611 PSAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00160 SerC_aminotrans_5, 1 hit
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR022278 Pser_aminoTfrase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
PIRSFiPIRSF000525 SerC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01364 serC_1, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit

Sequencei

Sequence statusi: Complete.

P33330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEREEPQH FGAGPAQMPT PVLQQAAKDL INFNDIGLGI GEISHRSKDA
60 70 80 90 100
TKVIEDSKKH LIELLNIPDT HEVFYLQGGG TTGFSSVATN LAAAYVGKHG
110 120 130 140 150
KIAPAGYLVT GSWSQKSFEE AKRLHVPAEV IFNAKDYNNG KFGKIPDESL
160 170 180 190 200
WEDKIKGKAF SYVYLCENET VHGVEWPELP KCLVNDPNIE IVADLSSDIL
210 220 230 240 250
SRKIDVSQYG VIMAGAQKNI GLAGLTLYII KKSILKNISG ASDETLHELG
260 270 280 290 300
VPITPIAFDY PTVVKNNSAY NTIPIFTLHV MDLVFQHILK KGGVEAQQAE
310 320 330 340 350
NEEKAKILYE ALDANSDFYN VPVDPKCRSK MNVVFTLKKD GLDDQFLKEA
360 370 380 390
AARHLTGLKG HRSVGGFRAS IYNALSVKAV QNLVDFIKEF AEKNA
Length:395
Mass (Da):43,416
Last modified:February 1, 1994 - v1
Checksum:iA22F020CC969B4BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti258 – 259FD → LH in AAA85703 (PubMed:7553933).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20917 Genomic DNA Translation: AAA20886.1
U19714 Genomic DNA Translation: AAA85703.1
Z75092 Genomic DNA Translation: CAA99393.1
BK006948 Genomic DNA Translation: DAA10956.1
PIRiS42680
RefSeqiNP_014827.3, NM_001183603.3

Genome annotation databases

EnsemblFungiiYOR184W; YOR184W; YOR184W
GeneIDi854356
KEGGisce:YOR184W

Similar proteinsi

Entry informationi

Entry nameiSERC_YEAST
AccessioniPrimary (citable) accession number: P33330
Secondary accession number(s): D6W2P0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health