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P33329

- PPZ2_YEAST

UniProt

P33329 - PPZ2_YEAST

Protein

Serine/threonine-protein phosphatase PP-Z2

Gene

PPZ2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Essential for the maintenance of cell size and integrity in response to osmotic stress.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi454 – 4541Manganese 1By similarity
    Metal bindingi456 – 4561Manganese 1By similarity
    Metal bindingi482 – 4821Manganese 1By similarity
    Metal bindingi482 – 4821Manganese 2By similarity
    Metal bindingi514 – 5141Manganese 2By similarity
    Active sitei515 – 5151Proton donorBy similarity
    Metal bindingi563 – 5631Manganese 2By similarity
    Metal bindingi638 – 6381Manganese 2By similarity

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. magnesium-dependent protein serine/threonine phosphatase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. cellular sodium ion homeostasis Source: SGD
    2. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:YDR436W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP-Z2 (EC:3.1.3.16)
    Gene namesi
    Name:PPZ2
    Ordered Locus Names:YDR436W
    ORF Names:D9461.22
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    SGDiS000002844. PPZ2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 710709Serine/threonine-protein phosphatase PP-Z2PRO_0000058892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis
    Modified residuei71 – 711Phosphoserine1 Publication
    Modified residuei203 – 2031Phosphoserine1 Publication
    Modified residuei224 – 2241Phosphoserine1 Publication
    Modified residuei310 – 3101Phosphoserine1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiP33329.
    PaxDbiP33329.
    PRIDEiP33329.

    Expressioni

    Gene expression databases

    GenevestigatoriP33329.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAB3P360764EBI-13815,EBI-26778

    Protein-protein interaction databases

    BioGridi32492. 51 interactions.
    DIPiDIP-6355N.
    IntActiP33329. 12 interactions.
    MINTiMINT-694847.
    STRINGi4932.YDR436W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33329.
    SMRiP33329. Positions 396-688.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi346 – 3538Poly-Ser

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00710000107291.
    HOGENOMiHOG000172697.
    KOiK06269.
    OMAiYSTPLHS.
    OrthoDBiEOG79KPQ9.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33329-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNSGSKQHT KHNSKKDDHD GDRKKTLDLP PLTKSDTTHS LKSSRSLRSL    50
    RSKRSEASLA SNVQAQTQPL SRRSSTLGNG NRNHRRSNNA PITPPNNHYL 100
    TSHPSSSRRL SSSSRRSSMG NNNNSELPPS MIQMEPKSPI LKNSTSMHST 150
    SSFNSYENAL TDDDDDRGDD GGESPSMAKV TRINTSSSAD RGSKRTPLRR 200
    HNSLQPEKGV TGFSSTSSKL RRRSDNTLPA SYPLNAEAGG NGSDYFSNRS 250
    NSHASSRKSS FGSTGNTAYS TPLHSPALRK MSSRDNDDSG DNVNGRGTSP 300
    IPNLNIDKPS PSASSASKRE YLSAYPTLAH RDSSSSLSPR GKGQRSSSSS 350
    SSSQRIYVSP PSPTGDFVHG SCADGDNGSR TNTMVEMKRK KPVRPVDIDE 400
    IIQRLLDAGY AAKRTKNVCL KNSEIIQICH KARELFLAQP ALLELSPSVK 450
    IVGDVHGQYA DLLRLFTKCG FPPMANYLFL GDYVDRGKQS LETILLLLCY 500
    KIKYPENFFL LRGNHECANV TRVYGFYDEC KRRCNIKIWK TFVDTFNTLP 550
    LAAIVTGKIF CVHGGLSPVL NSMDEIRHVS RPTDVPDFGL INDLLWSDPT 600
    DSSNEWEDNE RGVSFCYNKV AINKFLNKFG FDLVCRAHMV VEDGYEFFND 650
    RSLVTVFSAP NYCGEFDNWG AVMTVSEGLL CSFELLDPLD STALKQVMKK 700
    GRQERKLANR 710
    Length:710
    Mass (Da):78,492
    Last modified:January 23, 2007 - v4
    Checksum:i3095F37046489AFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti231 – 2311S → T in AAA34899. (PubMed:8395014)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74136 Genomic DNA. Translation: CAA52233.1.
    L10241 Genomic DNA. Translation: AAA34899.1.
    U33007 Genomic DNA. Translation: AAB64859.1.
    BK006938 Genomic DNA. Translation: DAA12273.1.
    PIRiS35674.
    RefSeqiNP_010724.1. NM_001180744.1.

    Genome annotation databases

    EnsemblFungiiYDR436W; YDR436W; YDR436W.
    GeneIDi852046.
    KEGGisce:YDR436W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74136 Genomic DNA. Translation: CAA52233.1 .
    L10241 Genomic DNA. Translation: AAA34899.1 .
    U33007 Genomic DNA. Translation: AAB64859.1 .
    BK006938 Genomic DNA. Translation: DAA12273.1 .
    PIRi S35674.
    RefSeqi NP_010724.1. NM_001180744.1.

    3D structure databases

    ProteinModelPortali P33329.
    SMRi P33329. Positions 396-688.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32492. 51 interactions.
    DIPi DIP-6355N.
    IntActi P33329. 12 interactions.
    MINTi MINT-694847.
    STRINGi 4932.YDR436W.

    Proteomic databases

    MaxQBi P33329.
    PaxDbi P33329.
    PRIDEi P33329.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR436W ; YDR436W ; YDR436W .
    GeneIDi 852046.
    KEGGi sce:YDR436W.

    Organism-specific databases

    SGDi S000002844. PPZ2.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00710000107291.
    HOGENOMi HOG000172697.
    KOi K06269.
    OMAi YSTPLHS.
    OrthoDBi EOG79KPQ9.

    Enzyme and pathway databases

    BioCyci YEAST:YDR436W-MONOMER.

    Miscellaneous databases

    NextBioi 970299.

    Gene expression databases

    Genevestigatori P33329.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A pair of functionally redundant yeast genes (PPZ1 and PPZ2) encoding type 1-related protein phosphatases function within the PKC1-mediated pathway."
      Lee K.S., Hines L.K., Levin D.E.
      Mol. Cell. Biol. 13:5843-5853(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Both isoforms of protein phosphatase Z are essential for the maintenance of cell size and integrity in Saccharomyces cerevisiae in response to osmotic stress."
      Hughes V., Mueller A., Stark M.J.R., Cohen P.T.W.
      Eur. J. Biochem. 216:269-279(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AY926.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Protein phosphatase 2Bw and protein phosphatase Z are Saccharomyces cerevisiae enzymes."
      Da Cruz e Silva E.F., Hughes V., McDonald P., Stark M.J.R., Cohen P.T.W.
      Biochim. Biophys. Acta 1089:269-272(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 424-645.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-224 AND SER-310, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPZ2_YEAST
    AccessioniPrimary (citable) accession number: P33329
    Secondary accession number(s): D6VT63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 144 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 189 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3