Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Synaptobrevin homolog 2

Gene

SNC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNC1 and SNC2 are vesicle-targeting proteins essential for normal secretory traffic between the Golgi and the plasma membrane. They may also be involved in vesicle fusion.

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • endocytosis Source: SGD
  • exocytosis Source: SGD
  • Golgi to plasma membrane transport Source: SGD
  • vesicle fusion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33804-MONOMER.
ReactomeiR-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptobrevin homolog 2
Gene namesi
Name:SNC2
Ordered Locus Names:YOR327C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR327C.
SGDiS000005854. SNC2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9393CytoplasmicSequence analysisAdd
BLAST
Transmembranei94 – 11219Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini113 – 1153VesicularSequence analysis

GO - Cellular componenti

  • endosome Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • SNARE complex Source: SGD
  • trans-Golgi network Source: SGD
  • transport vesicle membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 115115Synaptobrevin homolog 2PRO_0000206745Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581PhosphoserineCombined sources
Cross-linki62 – 62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication
Lipidationi94 – 941S-palmitoyl cysteineCurated

Post-translational modificationi

Palmitoylated by SWF1.2 Publications

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP33328.
TopDownProteomicsiP33328.

PTM databases

iPTMnetiP33328.
SwissPalmiP33328.

Interactioni

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi34712. 133 interactions.
DIPiDIP-4667N.
IntActiP33328. 10 interactions.
MINTiMINT-477413.

Structurei

3D structure databases

ProteinModelPortaliP33328.
SMRiP33328. Positions 28-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
InParanoidiP33328.
KOiK08513.
OMAiKATNYAN.
OrthoDBiEOG7W9S5V.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSVPYDPY VPPEESNSGA NPNSQNKTAA LRQEIDDTVG IMRDNINKVA
60 70 80 90 100
ERGERLTSIE DKADNLAISA QGFKRGANRV RKQMWWKDLK MRMCLFLVVI
110
ILLVVIIVPI VVHFS
Length:115
Mass (Da):12,957
Last modified:October 1, 1996 - v2
Checksum:i815F027FAD8A599B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151S → T in AAA19816 (PubMed:8374953).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16243 Unassigned DNA. Translation: AAA19816.1.
Z49821 Genomic DNA. Translation: CAA89974.1.
Z75235 Genomic DNA. Translation: CAA99647.1.
BK006948 Genomic DNA. Translation: DAA11090.1.
PIRiS62059.
RefSeqiNP_014972.3. NM_001183747.3.

Genome annotation databases

EnsemblFungiiYOR327C; YOR327C; YOR327C.
GeneIDi854505.
KEGGisce:YOR327C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16243 Unassigned DNA. Translation: AAA19816.1.
Z49821 Genomic DNA. Translation: CAA89974.1.
Z75235 Genomic DNA. Translation: CAA99647.1.
BK006948 Genomic DNA. Translation: DAA11090.1.
PIRiS62059.
RefSeqiNP_014972.3. NM_001183747.3.

3D structure databases

ProteinModelPortaliP33328.
SMRiP33328. Positions 28-85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34712. 133 interactions.
DIPiDIP-4667N.
IntActiP33328. 10 interactions.
MINTiMINT-477413.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiP33328.
SwissPalmiP33328.

Proteomic databases

MaxQBiP33328.
TopDownProteomicsiP33328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR327C; YOR327C; YOR327C.
GeneIDi854505.
KEGGisce:YOR327C.

Organism-specific databases

EuPathDBiFungiDB:YOR327C.
SGDiS000005854. SNC2.

Phylogenomic databases

GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
InParanoidiP33328.
KOiK08513.
OMAiKATNYAN.
OrthoDBiEOG7W9S5V.

Enzyme and pathway databases

BioCyciYEAST:G3O-33804-MONOMER.
ReactomeiR-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiP33328.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae."
    Protopopov V., Govidan B., Novick P., Gerst J.E.
    Cell 74:855-861(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: S288c / GRF88.
  2. "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
    Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
    Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-62.
    Strain: SUB592.
  7. "Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation."
    Valdez-Taubas J., Pelham H.R.B.
    EMBO J. 24:2524-2532(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  8. Cited for: PALMITOYLATION.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSNC2_YEAST
AccessioniPrimary (citable) accession number: P33328
Secondary accession number(s): D6W324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.