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Protein

Transketolase 2

Gene

TKL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.By similarity

Catalytic activityi

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Ca2+By similarity, Mn2+By similarity, Co2+By similarityNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30SubstrateBy similarity1
Sitei30Important for catalytic activityBy similarity1
Binding sitei69Thiamine pyrophosphateBy similarity1
Metal bindingi157MagnesiumBy similarity1
Binding sitei158Thiamine pyrophosphate; via amide nitrogenBy similarity1
Metal bindingi187MagnesiumBy similarity1
Binding sitei187Thiamine pyrophosphateBy similarity1
Metal bindingi189Magnesium; via carbonyl oxygenBy similarity1
Binding sitei263SubstrateBy similarity1
Binding sitei263Thiamine pyrophosphateBy similarity1
Sitei263Important for catalytic activityBy similarity1
Binding sitei359SubstrateBy similarity1
Binding sitei386SubstrateBy similarity1
Active sitei418Proton donorBy similarity1
Binding sitei418Thiamine pyrophosphateBy similarity1
Binding sitei445Thiamine pyrophosphateBy similarity1
Binding sitei469SubstrateBy similarity1
Binding sitei477SubstrateBy similarity1
Binding sitei528SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 118Thiamine pyrophosphateBy similarity3

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transketolase activity Source: SGD

GO - Biological processi

  • pentose-phosphate shunt Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YBR117C-MONOMER.
BRENDAi2.2.1.1. 984.
SABIO-RKP33315.

Names & Taxonomyi

Protein namesi
Recommended name:
Transketolase 2 (EC:2.2.1.1)
Short name:
TK 2
Gene namesi
Name:TKL2
Ordered Locus Names:YBR117C
ORF Names:YBR0912
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR117C.
SGDiS000000321. TKL2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001919052 – 681Transketolase 2Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei286PhosphoserineBy similarity1
Cross-linki648Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP33315.
PRIDEiP33315.
TopDownProteomicsiP33315.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32819. 19 interactors.
DIPiDIP-757N.
IntActiP33315. 2 interactors.
MINTiMINT-545508.

Structurei

3D structure databases

ProteinModelPortaliP33315.
SMRiP33315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transketolase family.Curated

Phylogenomic databases

HOGENOMiHOG000225953.
InParanoidiP33315.
KOiK00615.
OMAiHTTASER.
OrthoDBiEOG092C0ZWN.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR005478. Transketolase_bac-like.
IPR020826. Transketolase_BS.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
IPR005474. Transketolase_N.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 2 hits.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
PF00456. Transketolase_N. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR00232. tktlase_bact. 1 hit.
PROSITEiPS00801. TRANSKETOLASE_1. 1 hit.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC
60 70 80 90 100
NPNNEHWINR DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT
110 120 130 140 150
PGHPEFHSAG VEITSGPLGQ GISNAVGMAI AQANFAATYN EDGFPISDSY
160 170 180 190 200
TFAIVGDGCL QEGVSSETSS LAGHLQLGNL ITFYDSNSIS IDGKTSYSFD
210 220 230 240 250
EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK PTIIKVTTTI
260 270 280 290 300
GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT
310 320 330 340 350
VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT
360 370 380 390 400
PDDDALATRK TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP
410 420 430 440 450
ITQLGNYAGR YIRYGVREHG MGAIMNGISA FGANYKPYGG TFLNFVSYAA
460 470 480 490 500
GAVRLAALSG NPVIWVATHD SIGLGEDGPT HQPIETLAHL RAIPNMHVWR
510 520 530 540 550
PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE KALKGGYVIH
560 570 580 590 600
DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE
610 620 630 640 650
EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF
660 670 680
DFTADGVASR AEKTINYYKG KQLLSPMGRA F
Length:681
Mass (Da):75,029
Last modified:February 1, 1994 - v1
Checksum:i84D7477916D136D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73532 Genomic DNA. Translation: CAA51937.1.
X78993 Genomic DNA. Translation: CAA55619.1.
Z35985 Genomic DNA. Translation: CAA85074.1.
BK006936 Genomic DNA. Translation: DAA07235.1.
PIRiS37809.
RefSeqiNP_009675.3. NM_001178465.3.

Genome annotation databases

EnsemblFungiiYBR117C; YBR117C; YBR117C.
GeneIDi852414.
KEGGisce:YBR117C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73532 Genomic DNA. Translation: CAA51937.1.
X78993 Genomic DNA. Translation: CAA55619.1.
Z35985 Genomic DNA. Translation: CAA85074.1.
BK006936 Genomic DNA. Translation: DAA07235.1.
PIRiS37809.
RefSeqiNP_009675.3. NM_001178465.3.

3D structure databases

ProteinModelPortaliP33315.
SMRiP33315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32819. 19 interactors.
DIPiDIP-757N.
IntActiP33315. 2 interactors.
MINTiMINT-545508.

Proteomic databases

MaxQBiP33315.
PRIDEiP33315.
TopDownProteomicsiP33315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR117C; YBR117C; YBR117C.
GeneIDi852414.
KEGGisce:YBR117C.

Organism-specific databases

EuPathDBiFungiDB:YBR117C.
SGDiS000000321. TKL2.

Phylogenomic databases

HOGENOMiHOG000225953.
InParanoidiP33315.
KOiK00615.
OMAiHTTASER.
OrthoDBiEOG092C0ZWN.

Enzyme and pathway databases

BioCyciYEAST:YBR117C-MONOMER.
BRENDAi2.2.1.1. 984.
SABIO-RKP33315.

Miscellaneous databases

PROiP33315.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR005478. Transketolase_bac-like.
IPR020826. Transketolase_BS.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
IPR005474. Transketolase_N.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 2 hits.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
PF00456. Transketolase_N. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR00232. tktlase_bact. 1 hit.
PROSITEiPS00801. TRANSKETOLASE_1. 1 hit.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTKT2_YEAST
AccessioniPrimary (citable) accession number: P33315
Secondary accession number(s): D6VQB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.