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Protein

Inhibitory regulator protein BUD2/CLA2

Gene

BUD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates the GTPase activity of BUD1/RSR1. Participates in the regulation of bud-site selection.

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: SGD

GO - Biological processi

  • axial cellular bud site selection Source: SGD
  • bipolar cellular bud site selection Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • filamentous growth Source: SGD
  • invasive filamentous growth Source: SGD
  • mitotic spindle orientation checkpoint Source: SGD
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • signal transduction Source: InterPro

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-31883-MONOMER
ReactomeiR-SCE-5218921 VEGFR2 mediated cell proliferation
R-SCE-5658442 Regulation of RAS by GAPs
R-SCE-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein BUD2/CLA2
Alternative name(s):
Bud site selection protein 2
Gene namesi
Name:BUD2
Synonyms:CLA2, ERC25
Ordered Locus Names:YKL092C
ORF Names:YKL424
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL092C
SGDiS000001575 BUD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000566562 – 1104Inhibitory regulator protein BUD2/CLA2Add BLAST1103

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei854PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP33314
PaxDbiP33314
PRIDEiP33314

PTM databases

iPTMnetiP33314

Interactioni

Protein-protein interaction databases

BioGridi3404165 interactors.
DIPiDIP-2689N
IntActiP33314 24 interactors.
MINTiP33314
STRINGi4932.YKL092C

Structurei

3D structure databases

ProteinModelPortaliP33314
SMRiP33314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini322 – 425C2PROSITE-ProRule annotationAdd BLAST104
Domaini505 – 721Ras-GAPPROSITE-ProRule annotationAdd BLAST217

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi496 – 499Poly-Ser4
Compositional biasi1096 – 1099Poly-Lys4

Phylogenomic databases

GeneTreeiENSGT00760000119092
HOGENOMiHOG000095279
InParanoidiP33314
OMAiIGQEYLE
OrthoDBiEOG092C09G5

Family and domain databases

Gene3Di2.60.40.1501 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00616 RasGAP, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNNEPAQS RTSYFKLNEF LSNVKHYKNT FKGEIQWCNN LSLNDWKTHY
60 70 80 90 100
LQITSTGALT HSIDELTADS TNIQPIIKHL QQCRIEIIKD KHSSFKDINA
110 120 130 140 150
NCNFIIQVNT SGKDNKVYLR VKSWSDFKKL LTCLIWWSSM KTNGIFNKFQ
160 170 180 190 200
VSRPLEFKSK KMAKPESLLV YKLNVFGPIV KNIVLPPATN ILESPDIINN
210 220 230 240 250
DDNSVGWFSA MGVLKSNGML DLLLQSDGSL IYSLNISQLL RSEIRILDSS
260 270 280 290 300
VLQSENSLFL GELPLLRSQL GLEKFRIENI ASAATNSSDI SQEIIVEFPL
310 320 330 340 350
RIDLEDCFIA LQSFARSEYL SITGSDKSND MKISNSFKIS ILEANFQSIN
360 370 380 390 400
LNDKNNTPWS IFTDITAWGH TWARTSMVSN SSNPFWREEF QFNELLRLTN
410 420 430 440 450
SYLEIKQLFH DLNNKKRLRL IGKIKITQEI INDTRYNKET RLPIMDVDNK
460 470 480 490 500
NFQIGTICIK ISSNLNFILP STNFVKLEKL LMNANLSMVS NLIYKSSSSM
510 520 530 540 550
ENDNKLTQTS IIFLDIFQSL SRIEEWFHVL IDKELAKIDG TVSRINQKNL
560 570 580 590 600
DSKHVFNSLF RGNSILTKSI EQYFFRVGNE YLSKALSAIL KEIIESNKSC
610 620 630 640 650
ELDPARVKEK DEVKKRKIIA DNYKRLYSWV TKIWKRLYAT SNDLPIEIRN
660 670 680 690 700
VLKIFRQKLE IICIDDTLQI ILNGISGLLF LRFFCPVILN PKLFKYVSQN
710 720 730 740 750
LNETARRNLT LISKVLLNLS TLTQFANKEP WLMKMNNFID KRHNDLLDYI
760 770 780 790 800
DKMTQKKLDF NSKILNLSST ISRPKLAIEQ TMLDDLPQIP YLLDKNLRET
810 820 830 840 850
EFVNLIVNFS QEDMTKMEKY NHMDNGGKGE LIEEEGLLSG SSLNLSVDKK
860 870 880 890 900
DLDSPIEVKP EIGELEFEKI TENNTEIFGD DLMNLLKSDD VGSRSRDLDN
910 920 930 940 950
GANSGIKFNS IIPKAEEEKH AMKELEQESC LLYNRINHIR KRLSGYECAS
960 970 980 990 1000
STLFEDKKYS ISLSHKIFYE EIKEGKEIVL KLLNKPTNEN SSARLQKFFT
1010 1020 1030 1040 1050
KGVSSKSNNT VGDSYCKFLT IDVSDENPKS SNKTSVHGTS SENGAKDDYL
1060 1070 1080 1090 1100
TLPNSQGKGN LGNRFSPTKL SRIMRKPPNA DVPKEQNSRK LTRWFKKKKE

TGGS
Length:1,104
Mass (Da):126,663
Last modified:June 1, 1994 - v2
Checksum:i451AFC3A78384760
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti437N → Y in CAA52228 (PubMed:8262070).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74130 Genomic DNA Translation: CAA52228.1
L19162 Genomic DNA Translation: AAA34461.1
X75561 Genomic DNA Translation: CAA53241.1
Z28092 Genomic DNA Translation: CAA81930.1
BK006944 Genomic DNA Translation: DAA09066.1
PIRiS36773
RefSeqiNP_012831.1, NM_001179658.1

Genome annotation databases

EnsemblFungiiYKL092C; YKL092C; YKL092C
GeneIDi853770
KEGGisce:YKL092C

Similar proteinsi

Entry informationi

Entry nameiBUD2_YEAST
AccessioniPrimary (citable) accession number: P33314
Secondary accession number(s): D6VXJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome