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Protein

Inhibitory regulator protein BUD2/CLA2

Gene

BUD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the GTPase activity of BUD1/RSR1. Participates in the regulation of bud-site selection.

GO - Molecular functioni

  • GTPase activator activity Source: SGD

GO - Biological processi

  • axial cellular bud site selection Source: SGD
  • bipolar cellular bud site selection Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • filamentous growth Source: SGD
  • invasive filamentous growth Source: SGD
  • mitotic spindle orientation checkpoint Source: SGD
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-31883-MONOMER.
ReactomeiR-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5658442. Regulation of RAS by GAPs.
R-SCE-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein BUD2/CLA2
Alternative name(s):
Bud site selection protein 2
Gene namesi
Name:BUD2
Synonyms:CLA2, ERC25
Ordered Locus Names:YKL092C
ORF Names:YKL424
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL092C.
SGDiS000001575. BUD2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 11041103Inhibitory regulator protein BUD2/CLA2PRO_0000056656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei854 – 8541PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP33314.

PTM databases

iPTMnetiP33314.

Interactioni

Protein-protein interaction databases

BioGridi34041. 25 interactions.
DIPiDIP-2689N.
IntActiP33314. 9 interactions.
MINTiMINT-583817.

Structurei

3D structure databases

ProteinModelPortaliP33314.
SMRiP33314. Positions 559-740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini322 – 425104C2PROSITE-ProRule annotationAdd
BLAST
Domaini505 – 721217Ras-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi496 – 4994Poly-Ser
Compositional biasi1096 – 10994Poly-Lys

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119092.
HOGENOMiHOG000095279.
InParanoidiP33314.
OMAiFFCPVIL.
OrthoDBiEOG092C09G5.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNNEPAQS RTSYFKLNEF LSNVKHYKNT FKGEIQWCNN LSLNDWKTHY
60 70 80 90 100
LQITSTGALT HSIDELTADS TNIQPIIKHL QQCRIEIIKD KHSSFKDINA
110 120 130 140 150
NCNFIIQVNT SGKDNKVYLR VKSWSDFKKL LTCLIWWSSM KTNGIFNKFQ
160 170 180 190 200
VSRPLEFKSK KMAKPESLLV YKLNVFGPIV KNIVLPPATN ILESPDIINN
210 220 230 240 250
DDNSVGWFSA MGVLKSNGML DLLLQSDGSL IYSLNISQLL RSEIRILDSS
260 270 280 290 300
VLQSENSLFL GELPLLRSQL GLEKFRIENI ASAATNSSDI SQEIIVEFPL
310 320 330 340 350
RIDLEDCFIA LQSFARSEYL SITGSDKSND MKISNSFKIS ILEANFQSIN
360 370 380 390 400
LNDKNNTPWS IFTDITAWGH TWARTSMVSN SSNPFWREEF QFNELLRLTN
410 420 430 440 450
SYLEIKQLFH DLNNKKRLRL IGKIKITQEI INDTRYNKET RLPIMDVDNK
460 470 480 490 500
NFQIGTICIK ISSNLNFILP STNFVKLEKL LMNANLSMVS NLIYKSSSSM
510 520 530 540 550
ENDNKLTQTS IIFLDIFQSL SRIEEWFHVL IDKELAKIDG TVSRINQKNL
560 570 580 590 600
DSKHVFNSLF RGNSILTKSI EQYFFRVGNE YLSKALSAIL KEIIESNKSC
610 620 630 640 650
ELDPARVKEK DEVKKRKIIA DNYKRLYSWV TKIWKRLYAT SNDLPIEIRN
660 670 680 690 700
VLKIFRQKLE IICIDDTLQI ILNGISGLLF LRFFCPVILN PKLFKYVSQN
710 720 730 740 750
LNETARRNLT LISKVLLNLS TLTQFANKEP WLMKMNNFID KRHNDLLDYI
760 770 780 790 800
DKMTQKKLDF NSKILNLSST ISRPKLAIEQ TMLDDLPQIP YLLDKNLRET
810 820 830 840 850
EFVNLIVNFS QEDMTKMEKY NHMDNGGKGE LIEEEGLLSG SSLNLSVDKK
860 870 880 890 900
DLDSPIEVKP EIGELEFEKI TENNTEIFGD DLMNLLKSDD VGSRSRDLDN
910 920 930 940 950
GANSGIKFNS IIPKAEEEKH AMKELEQESC LLYNRINHIR KRLSGYECAS
960 970 980 990 1000
STLFEDKKYS ISLSHKIFYE EIKEGKEIVL KLLNKPTNEN SSARLQKFFT
1010 1020 1030 1040 1050
KGVSSKSNNT VGDSYCKFLT IDVSDENPKS SNKTSVHGTS SENGAKDDYL
1060 1070 1080 1090 1100
TLPNSQGKGN LGNRFSPTKL SRIMRKPPNA DVPKEQNSRK LTRWFKKKKE

TGGS
Length:1,104
Mass (Da):126,663
Last modified:June 1, 1994 - v2
Checksum:i451AFC3A78384760
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371N → Y in CAA52228 (PubMed:8262070).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74130 Genomic DNA. Translation: CAA52228.1.
L19162 Genomic DNA. Translation: AAA34461.1.
X75561 Genomic DNA. Translation: CAA53241.1.
Z28092 Genomic DNA. Translation: CAA81930.1.
BK006944 Genomic DNA. Translation: DAA09066.1.
PIRiS36773.
RefSeqiNP_012831.1. NM_001179658.1.

Genome annotation databases

EnsemblFungiiYKL092C; YKL092C; YKL092C.
GeneIDi853770.
KEGGisce:YKL092C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74130 Genomic DNA. Translation: CAA52228.1.
L19162 Genomic DNA. Translation: AAA34461.1.
X75561 Genomic DNA. Translation: CAA53241.1.
Z28092 Genomic DNA. Translation: CAA81930.1.
BK006944 Genomic DNA. Translation: DAA09066.1.
PIRiS36773.
RefSeqiNP_012831.1. NM_001179658.1.

3D structure databases

ProteinModelPortaliP33314.
SMRiP33314. Positions 559-740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34041. 25 interactions.
DIPiDIP-2689N.
IntActiP33314. 9 interactions.
MINTiMINT-583817.

PTM databases

iPTMnetiP33314.

Proteomic databases

MaxQBiP33314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL092C; YKL092C; YKL092C.
GeneIDi853770.
KEGGisce:YKL092C.

Organism-specific databases

EuPathDBiFungiDB:YKL092C.
SGDiS000001575. BUD2.

Phylogenomic databases

GeneTreeiENSGT00760000119092.
HOGENOMiHOG000095279.
InParanoidiP33314.
OMAiFFCPVIL.
OrthoDBiEOG092C09G5.

Enzyme and pathway databases

BioCyciYEAST:G3O-31883-MONOMER.
ReactomeiR-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5658442. Regulation of RAS by GAPs.
R-SCE-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiP33314.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUD2_YEAST
AccessioniPrimary (citable) accession number: P33314
Secondary accession number(s): D6VXJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.