P33311 (MDL2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent permease MDL2, mitochondrial

Alternative name(s):
Multidrug resistance-like protein 2

Gene names

Name:	MDL2
Synonyms:	SSH1
Ordered Locus Names:	YPL270W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Ref.5 Ref.7 Ref.9.
Miscellaneous	Present with 3390 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
Sequence caution	The sequence AAA20682.1 differs from that shown. Reason: Frameshift at position 754. The sequence AAT93160.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA98006.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oligopeptide export from mitochondrion Inferred from sequence alignment Ref.5. Source: SGD
Cellular_component	integral to membrane Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrial inner membrane Inferred from direct assay Ref.5. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW oligopeptide-transporting ATPase activity Inferred from sequence alignment Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 90	90	Mitochondrion Potential
	Chain	91 – 773	683	ATP-dependent permease MDL2, mitochondrial		PRO_0000045331
Regions
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	170 – 192	23	Helical; Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Domain	119 – 413	295	ABC transmembrane type-1
	Domain	493 – 733	241	ABC transporter
	Nucleotide binding	481 – 488	8	ATP Potential
Experimental info
	Sequence conflict	56	1	G → A in AAA20682. Ref.1
	Sequence conflict	265	1	S → C in AAA20682. Ref.1
	Sequence conflict	752	1	S → C in AAA20682. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33311 [UniParc]. Last modified December 20, 2005. Version 3. Checksum: 58B1DF92D60E1E58	FASTA	773	85,137
	10 20 30 40 50 60 MLNGRLPLLR LGICRNMLSR PRLAKLPSIR FRSLVTPSSS QLIPLSRLCL RSPAVGKSLI 70 80 90 100 110 120 LQSFRCNSSK TVPETSLPSA SPISKGSARS AHAKEQSKTD DYKDIIRLFM LAKRDWKLLL 130 140 150 160 170 180 TAILLLTISC SIGMSIPKVI GIVLDTLKTS SGSDFFDLKI PIFSLPLYEF LSFFTVALLI 190 200 210 220 230 240 GCAANFGRFI LLRILSERVV ARLRANVIKK TLHQDAEFFD NHKVGDLISR LGSDAYVVSR 250 260 270 280 290 300 SMTQKVSDGV KALICGVVGV GMMCSLSPQL SILLLFFTPP VLFSASVFGK QIRNTSKDLQ 310 320 330 340 350 360 EATGQLTRVA EEQLSGIKTV QSFVAEGNEL SRYNVAIRDI FQVGKTAAFT NAKFFTTTSL 370 380 390 400 410 420 LGDLSFLTVL AYGSYLVLQS QLSIGDLTAF MLYTEYTGNA VFGLSTFYSE IMQGAGAASR 430 440 450 460 470 480 LFELTDRKPS ISPTVGHKYK PDRGVIEFKD VSFSYPTRPS VQIFKNLNFK IAPGSSVCIV 490 500 510 520 530 540 GPSGRGKSTI ALLLLRYYNP TTGTITIDNQ DISKLNCKSL RRHIGIVQQE PVLMSGTIRD 550 560 570 580 590 600 NITYGLTYTP TKEEIRSVAK QCFCHNFITK FPNTYDTVIG PHGTLLSGGQ KQRIAIARAL 610 620 630 640 650 660 IKKPTILILD EATSALDVES EGAINYTFGQ LMKSKSMTIV SIAHRLSTIR RSENVIVLGH 670 680 690 700 710 720 DGSVVEMGKF KELYANPTSA LSQLLNEKAA PGPSDQQLQI EKVIEKEDLN ESKEHDDQKK 730 740 750 760 770 DDNDDNDNNH DNDSNNQSPE TKDNNSDDIE KSVEHLLKDA AKEANPIKIT PQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily." Dean M.C., Allikmets R., Gerrard B.C., Stewart C., Kistler A., Shafer B., Michaelis S., Strathern J. Yeast 10:377-383(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Role of the ABC transporter Mdl1 in peptide export from mitochondria." Young L., Leonhard K., Tatsuta T., Trowsdale J., Langer T. Science 291:2135-2138(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16959 Genomic DNA. Translation: AAA20682.1. Frameshift. Z73626 Genomic DNA. Translation: CAA98006.1. Different initiation. AY693141 Genomic DNA. Translation: AAT93160.1. Different initiation. BK006949 Genomic DNA. Translation: DAA11166.1.
PIR	S65303.
RefSeq	NP_015053.2. NM_001184084.1.
3D structure databases
ProteinModelPortal	P33311.
SMR	P33311. Positions 107-675.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4226N.
MINT	MINT-509434.
STRING	4932.YPL270W.
Protein family/group databases
TCDB	3.A.1.212.2. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PaxDb	P33311.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL270W; YPL270W; YPL270W.
GeneID	855858.
KEGG	sce:YPL270W.
Organism-specific databases
CYGD	YPL270w.
SGD	S000006191. MDL2.
Phylogenomic databases
eggNOG	COG1132.
GeneTree	ENSGT00550000074497.
KO	K02021.
OMA	LPQEFIE.
OrthoDB	EOG4T1MW2.
Gene expression databases
Genevestigator	P33311.
GermOnline	YPL270W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR005293. Ag_transporter2. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
TIGRFAMs	TIGR00958. 3a01208. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980469.

Entry information

Entry name

MDL2_YEAST

Accession

Primary (citable) accession number: P33311
Secondary accession number(s): D6W3A0, Q08983

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program