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Protein

ATP-dependent permease MDL1, mitochondrial

Gene

MDL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates export of peptides with molecular masses of 2100 to 600 daltons generated upon proteolysis of mitochondrial inner membrane proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi467 – 4748ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • oligopeptide-transporting ATPase activity Source: SGD

GO - Biological processi

  • oligopeptide export from mitochondrion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32311-MONOMER.
ReactomeiR-SCE-382556. ABC-family proteins mediated transport.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Protein family/group databases

TCDBi3.A.1.212.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent permease MDL1, mitochondrial
Alternative name(s):
ABC transporter MDL1
Multidrug resistance-like protein 1
Gene namesi
Name:MDL1
Ordered Locus Names:YLR188W
ORF Names:L9470.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR188W.
SGDiS000004178. MDL1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei103 – 12321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei156 – 17621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei242 – 26221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei337 – 35721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei372 – 39221HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi467 – 4671G → V: Decreased release of long peptides from mitochondria. 1 Publication
Mutagenesisi575 – 5751S → N: Decreased release of long peptides from mitochondria. 1 Publication
Mutagenesisi598 – 5981D → A: Decreased release of long peptides from mitochondria. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 100100MitochondrionSequence analysisAdd
BLAST
Chaini101 – 695595ATP-dependent permease MDL1, mitochondrialPRO_0000045330Add
BLAST

Proteomic databases

MaxQBiP33310.
PeptideAtlasiP33310.

Interactioni

Protein-protein interaction databases

BioGridi31458. 77 interactions.
MINTiMINT-4495270.

Structurei

3D structure databases

ProteinModelPortaliP33310.
SMRiP33310. Positions 88-677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 398296ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini432 – 673242ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074497.
InParanoidiP33310.
KOiK02021.
OMAiKEIWRLL.
OrthoDBiEOG74BK1Z.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030279. Mdl1/Mdl2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24221:SF198. PTHR24221:SF198. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVRMIRLCK GPKLLRSQFA SASALYSTKS LFKPPMYQKA EINLIIPHRK
60 70 80 90 100
HFLLRSIRLQ SDIAQGKKST KPTLKLSNAN SKSSGFKDIK RLFVLSKPES
110 120 130 140 150
KYIGLALLLI LISSSVSMAV PSVIGKLLDL ASESDGEDEE GSKSNKLYGF
160 170 180 190 200
TKKQFFTALG AVFIIGAVAN ASRIIILKVT GERLVARLRT RTMKAALDQD
210 220 230 240 250
ATFLDTNRVG DLISRLSSDA SIVAKSVTQN VSDGTRAIIQ GFVGFGMMSF
260 270 280 290 300
LSWKLTCVMM ILAPPLGAMA LIYGRKIRNL SRQLQTSVGG LTKVAEEQLN
310 320 330 340 350
ATRTIQAYGG EKNEVRRYAK EVRNVFHIGL KEAVTSGLFF GSTGLVGNTA
360 370 380 390 400
MLSLLLVGTS MIQSGSMTVG ELSSFMMYAV YTGSSLFGLS SFYSELMKGA
410 420 430 440 450
GAAARVFELN DRKPLIRPTI GKDPVSLAQK PIVFKNVSFT YPTRPKHQIF
460 470 480 490 500
KDLNITIKPG EHVCAVGPSG SGKSTIASLL LRYYDVNSGS IEFGDEDIRN
510 520 530 540 550
FNLRKYRRLI GYVQQEPLLF NGTILDNILY CIPPEIAEQD DRIRRAIGKA
560 570 580 590 600
NCTKFLANFP DGLQTMVGAR GAQLSGGQKQ RIALARAFLL DPAVLILDEA
610 620 630 640 650
TSALDSQSEE IVAKNLQRRV ERGFTTISIA HRLSTIKHST RVIVLGKHGS
660 670 680 690
VVETGSFRDL IAIPNSELNA LLAEQQDEEG KGGVIDLDNS VAREV
Length:695
Mass (Da):75,950
Last modified:February 1, 1996 - v2
Checksum:i3175B17FBD779BBE
GO

Sequence cautioni

The sequence AAA20681.1 differs from that shown. Reason: Frameshift at positions 417 and 433. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → G in AAA20681 (PubMed:7912468).Curated
Sequence conflicti150 – 1501F → L in AAA20681 (PubMed:7912468).Curated
Sequence conflicti267 – 2682GA → WP in AAA20681 (PubMed:7912468).Curated
Sequence conflicti313 – 3142NE → KQ in AAA20681 (PubMed:7912468).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16958 Genomic DNA. Translation: AAA20681.1. Frameshift.
U17246 Genomic DNA. Translation: AAB67455.1.
BK006945 Genomic DNA. Translation: DAA09507.1.
PIRiS51433.
RefSeqiNP_013289.1. NM_001182075.1.

Genome annotation databases

EnsemblFungiiYLR188W; YLR188W; YLR188W.
GeneIDi850885.
KEGGisce:YLR188W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16958 Genomic DNA. Translation: AAA20681.1. Frameshift.
U17246 Genomic DNA. Translation: AAB67455.1.
BK006945 Genomic DNA. Translation: DAA09507.1.
PIRiS51433.
RefSeqiNP_013289.1. NM_001182075.1.

3D structure databases

ProteinModelPortaliP33310.
SMRiP33310. Positions 88-677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31458. 77 interactions.
MINTiMINT-4495270.

Protein family/group databases

TCDBi3.A.1.212.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBiP33310.
PeptideAtlasiP33310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR188W; YLR188W; YLR188W.
GeneIDi850885.
KEGGisce:YLR188W.

Organism-specific databases

EuPathDBiFungiDB:YLR188W.
SGDiS000004178. MDL1.

Phylogenomic databases

GeneTreeiENSGT00550000074497.
InParanoidiP33310.
KOiK02021.
OMAiKEIWRLL.
OrthoDBiEOG74BK1Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-32311-MONOMER.
ReactomeiR-SCE-382556. ABC-family proteins mediated transport.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

PROiP33310.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030279. Mdl1/Mdl2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24221:SF198. PTHR24221:SF198. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily."
    Dean M.C., Allikmets R., Gerrard B.C., Stewart C., Kistler A., Shafer B., Michaelis S., Strathern J.
    Yeast 10:377-383(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Role of the ABC transporter Mdl1 in peptide export from mitochondria."
    Young L., Leonhard K., Tatsuta T., Trowsdale J., Langer T.
    Science 291:2135-2138(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-467; SER-575 AND ASP-598.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiMDL1_YEAST
AccessioniPrimary (citable) accession number: P33310
Secondary accession number(s): D6VYJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.