P33308 (MED9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 9

Alternative name(s):
Chromosome segregation protein 2
Mediator complex subunit 9

Gene names

Name:	CSE2
Synonyms:	MED9
Ordered Locus Names:	YNR010W
ORF Names:	N2046

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.7 Ref.13 Ref.14 Ref.15
Subunit structure	Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. CSE2/MED9 interacts directly with MED4. Ref.7 Ref.16
Subcellular location	Nucleus Ref.8.
Miscellaneous	Present with 1364 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Mediator complex subunit 9 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11470794. Source: SGD
Cellular_component	core mediator complex Inferred from direct assay Ref.6. Source: SGD mediator complex Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Inferred from direct assay PubMed 19077037. Source: SGD structural molecule activity Inferred from mutant phenotype PubMed 19077037. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Mediator of RNA polymerase II transcription subunit 9		PRO_0000076529
Regions
	Motif	77 – 99	23	Nuclear localization signal Potential
	Motif	136 – 149	14	Nuclear localization signal Potential

Sequences

	Sequence		Length	Mass (Da)
	P33308 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 99D2347F5287AA3C	FASTA	149	17,376
	10 20 30 40 50 60 MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ PKNLSTPSTS 70 80 90 100 110 120 SNGEFIPHIF YSLHQIRKDP NNLSNQLETL TGSIRHRLKL CKSLISENED TKDLLSKSPS 130 140 EWQDIIHQRE QELQIKRDVL DDLYRKLQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae." Xiao Z., McGrew J.T., Schroeder A.J., Fitzgerald-Hayes M. Mol. Cell. Biol. 13:4691-4702(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm." Verhasselt P., Aert R., Voet M., Volckaert G. Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae." Gustafsson C.M., Myers L.C., Beve J., Spaahr H., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF MEDIATOR COMPLEX.
[7]	"The structural and functional organization of the yeast mediator complex." Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J. J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II." Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D. Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[11]	"A high resolution protein interaction map of the yeast Mediator complex." Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M. Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[12]	"Preponderance of free mediator in the yeast Saccharomyces cerevisiae." Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J. J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[13]	"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts." Nair D., Kim Y., Myers L.C. J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]	"Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets." van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P. Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"Mediator as a general transcription factor." Takagi Y., Kornberg R.D. J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[16]	"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex." Baidoobonso S.M., Guidi B.W., Myers L.C. J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRB5, CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[17]	"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction." Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J. Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14839 Genomic DNA. Translation: AAA34532.1. X77395 Genomic DNA. Translation: CAA54578.1. Z71625 Genomic DNA. Translation: CAA96287.1. AY558422 Genomic DNA. Translation: AAS56748.1. BK006947 Genomic DNA. Translation: DAA10551.1.
PIR	B48083.
RefSeq	NP_014407.3. NM_001183187.3.
3D structure databases
ProteinModelPortal	P33308.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1276N.
IntAct	P33308. 12 interactions.
MINT	MINT-390653.
STRING	4932.YNR010W.
Proteomic databases
PaxDb	P33308.
PeptideAtlas	P33308.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR010W; YNR010W; YNR010W.
GeneID	855744.
KEGG	sce:YNR010W. sce:YNR017W.
Organism-specific databases
CYGD	YNR010w.
SGD	S000005293. CSE2.
Phylogenomic databases
eggNOG	NOG79103.
KO	K15150.
OMA	SKSPSEW.
OrthoDB	EOG4FFHBP.
Gene expression databases
Genevestigator	P33308.
GermOnline	YNR010W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011425. Mediator_Med9. [Graphical view]
Pfam	PF07544. Med9. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980145.

Entry information

Entry name

MED9_YEAST

Accession

Primary (citable) accession number: P33308
Secondary accession number(s): D6W1I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program