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P33308

- MED9_YEAST

UniProt

P33308 - MED9_YEAST

Protein

Mediator of RNA polymerase II transcription subunit 9

Gene

CSE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

    GO - Molecular functioni

    1. RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
    2. structural molecule activity Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33327-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 9
    Alternative name(s):
    Chromosome segregation protein 2
    Mediator complex subunit 9
    Gene namesi
    Name:CSE2
    Synonyms:MED9
    Ordered Locus Names:YNR010W
    ORF Names:N2046
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR010w.
    SGDiS000005293. CSE2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. core mediator complex Source: SGD
    2. cytosol Source: SGD
    3. mediator complex Source: InterPro
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 149149Mediator of RNA polymerase II transcription subunit 9PRO_0000076529Add
    BLAST

    Proteomic databases

    MaxQBiP33308.
    PaxDbiP33308.
    PeptideAtlasiP33308.

    Expressioni

    Gene expression databases

    GenevestigatoriP33308.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. CSE2/MED9 interacts directly with MED4.2 Publications

    Protein-protein interaction databases

    BioGridi35835. 478 interactions.
    DIPiDIP-1276N.
    IntActiP33308. 11 interactions.
    MINTiMINT-390653.
    STRINGi4932.YNR010W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi77 – 9923Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi136 – 14914Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 9 family.Curated

    Phylogenomic databases

    eggNOGiNOG79103.
    KOiK15150.
    OMAiSKSPSEW.
    OrthoDBiEOG7VHT8R.

    Family and domain databases

    InterProiIPR011425. Mediator_Med9.
    [Graphical view]
    PfamiPF07544. Med9. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33308-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ    50
    PKNLSTPSTS SNGEFIPHIF YSLHQIRKDP NNLSNQLETL TGSIRHRLKL 100
    CKSLISENED TKDLLSKSPS EWQDIIHQRE QELQIKRDVL DDLYRKLQR 149
    Length:149
    Mass (Da):17,376
    Last modified:February 1, 1994 - v1
    Checksum:i99D2347F5287AA3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14839 Genomic DNA. Translation: AAA34532.1.
    X77395 Genomic DNA. Translation: CAA54578.1.
    Z71625 Genomic DNA. Translation: CAA96287.1.
    AY558422 Genomic DNA. Translation: AAS56748.1.
    BK006947 Genomic DNA. Translation: DAA10551.1.
    PIRiB48083.
    RefSeqiNP_014407.3. NM_001183187.3.

    Genome annotation databases

    EnsemblFungiiYNR010W; YNR010W; YNR010W.
    GeneIDi855744.
    KEGGisce:YNR010W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14839 Genomic DNA. Translation: AAA34532.1 .
    X77395 Genomic DNA. Translation: CAA54578.1 .
    Z71625 Genomic DNA. Translation: CAA96287.1 .
    AY558422 Genomic DNA. Translation: AAS56748.1 .
    BK006947 Genomic DNA. Translation: DAA10551.1 .
    PIRi B48083.
    RefSeqi NP_014407.3. NM_001183187.3.

    3D structure databases

    ProteinModelPortali P33308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35835. 478 interactions.
    DIPi DIP-1276N.
    IntActi P33308. 11 interactions.
    MINTi MINT-390653.
    STRINGi 4932.YNR010W.

    Proteomic databases

    MaxQBi P33308.
    PaxDbi P33308.
    PeptideAtlasi P33308.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR010W ; YNR010W ; YNR010W .
    GeneIDi 855744.
    KEGGi sce:YNR010W.

    Organism-specific databases

    CYGDi YNR010w.
    SGDi S000005293. CSE2.

    Phylogenomic databases

    eggNOGi NOG79103.
    KOi K15150.
    OMAi SKSPSEW.
    OrthoDBi EOG7VHT8R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33327-MONOMER.

    Miscellaneous databases

    NextBioi 980145.

    Gene expression databases

    Genevestigatori P33308.

    Family and domain databases

    InterProi IPR011425. Mediator_Med9.
    [Graphical view ]
    Pfami PF07544. Med9. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae."
      Xiao Z., McGrew J.T., Schroeder A.J., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 13:4691-4702(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
      Verhasselt P., Aert R., Voet M., Volckaert G.
      Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae."
      Gustafsson C.M., Myers L.C., Beve J., Spaahr H., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPONENT OF MEDIATOR COMPLEX.
    7. "The structural and functional organization of the yeast mediator complex."
      Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
      J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. Cited for: NOMENCLATURE.
    11. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    12. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
      Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
      J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
    13. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    14. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
      van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
      Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "Mediator as a general transcription factor."
      Takagi Y., Kornberg R.D.
      J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    16. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
      Baidoobonso S.M., Guidi B.W., Myers L.C.
      J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB5, CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
      Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
      Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED9_YEAST
    AccessioniPrimary (citable) accession number: P33308
    Secondary accession number(s): D6W1I5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1364 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3