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P33307

- CSE1_YEAST

UniProt

P33307 - CSE1_YEAST

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Protein

Importin alpha re-exporter

Gene

CSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.4 Publications

GO - Molecular functioni

  1. importin-alpha export receptor activity Source: SGD

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. protein export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30711-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin alpha re-exporter
Alternative name(s):
Chromosome segregation protein CSE1
Gene namesi
Name:CSE1
Ordered Locus Names:YGL238W
ORF Names:HRC135
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL238w.
SGDiS000003207. CSE1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nuclear envelope Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Importin alpha re-exporterPRO_0000117293Add
BLAST

Proteomic databases

MaxQBiP33307.
PaxDbiP33307.
PeptideAtlasiP33307.

Expressioni

Gene expression databases

GenevestigatoriP33307.

Interactioni

Subunit structurei

Binds with high affinity to SRP1 only in the presence of RanGTP. The complex is dissociated by the RanGTP-binding protein YRB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SRP1Q028212EBI-5168,EBI-1797

Protein-protein interaction databases

BioGridi33001. 42 interactions.
DIPiDIP-1131N.
IntActiP33307. 22 interactions.
MINTiMINT-404965.
STRINGi4932.YGL238W.

Structurei

Secondary structure

1
960
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1311
Helixi17 – 193
Helixi20 – 3112
Helixi36 – 4510
Helixi51 – 6818
Beta strandi72 – 743
Beta strandi76 – 783
Helixi80 – 9617
Helixi99 – 11618
Turni118 – 1203
Helixi124 – 1296
Helixi139 – 15113
Helixi152 – 1543
Helixi161 – 18828
Helixi197 – 21721
Helixi223 – 2275
Helixi229 – 24012
Helixi245 – 2473
Beta strandi253 – 2553
Helixi258 – 27619
Helixi278 – 29821
Helixi303 – 3053
Helixi306 – 32015
Helixi323 – 3264
Helixi327 – 3293
Helixi332 – 34110
Helixi343 – 3475
Helixi351 – 3533
Turni354 – 3596
Helixi361 – 37010
Helixi377 – 39115
Helixi393 – 41220
Turni415 – 4173
Helixi419 – 43315
Beta strandi434 – 4363
Helixi452 – 4598
Helixi461 – 4655
Helixi472 – 48413
Helixi486 – 4883
Helixi491 – 50414
Helixi510 – 52314
Beta strandi530 – 5323
Beta strandi535 – 5373
Helixi539 – 5413
Turni542 – 5454
Helixi546 – 55813
Helixi564 – 5674
Helixi571 – 58414
Turni585 – 5884
Helixi589 – 5913
Helixi592 – 60615
Helixi613 – 62816
Helixi632 – 6343
Helixi635 – 65117
Turni655 – 6573
Helixi658 – 67114
Beta strandi672 – 6743
Turni677 – 6793
Helixi680 – 6823
Helixi683 – 6864
Helixi689 – 6924
Turni695 – 6973
Helixi698 – 71215
Helixi713 – 7153
Helixi720 – 73011
Turni733 – 7353
Helixi736 – 74914
Helixi752 – 7554
Helixi756 – 7583
Helixi759 – 76810
Helixi769 – 7713
Helixi776 – 79318
Helixi795 – 8039
Helixi809 – 8168
Helixi818 – 8214
Helixi822 – 8243
Helixi828 – 84316
Helixi846 – 8516
Helixi853 – 8553
Helixi856 – 86813
Helixi894 – 8963
Helixi899 – 9013
Turni914 – 9163
Helixi917 – 93216
Beta strandi934 – 9363
Helixi937 – 9415
Helixi942 – 9443
Helixi947 – 95711

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WA5X-ray2.00C1-960[»]
1Z3HX-ray3.10A/B1-960[»]
ProteinModelPortaliP33307.
SMRiP33307. Positions 1-958.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33307.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 3333HEAT 1Add
BLAST
Domaini23 – 9674Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati34 – 7340HEAT 2Add
BLAST
Repeati74 – 12047HEAT 3Add
BLAST
Repeati121 – 15737HEAT 4Add
BLAST
Repeati158 – 22063HEAT 5Add
BLAST
Repeati221 – 27858HEAT 6Add
BLAST
Repeati279 – 32345HEAT 7Add
BLAST
Repeati324 – 39269HEAT 8Add
BLAST
Repeati393 – 44553HEAT 9Add
BLAST
Repeati446 – 48944HEAT 10Add
BLAST
Repeati490 – 52839HEAT 11Add
BLAST
Repeati529 – 58658HEAT 12Add
BLAST
Repeati587 – 63044HEAT 13Add
BLAST
Repeati631 – 67444HEAT 14Add
BLAST
Repeati675 – 71642HEAT 15Add
BLAST
Repeati717 – 75135HEAT 16Add
BLAST
Repeati752 – 79443HEAT 17Add
BLAST
Repeati795 – 82632HEAT 18Add
BLAST
Repeati827 – 928102HEAT 19; with insertAdd
BLAST
Repeati929 – 96032HEAT 20Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi366 – 38116Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the XPO2/CSE1 family.Curated
Contains 20 HEAT repeats.Curated
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5657.
GeneTreeiENSGT00550000074884.
HOGENOMiHOG000201317.
InParanoidiP33307.
KOiK18423.
OMAiQFVTAVW.
OrthoDBiEOG78WM14.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR005043. CAS_CSE1_C.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03378. CAS_CSE1. 1 hit.
PF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33307-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDLETVAKF LAESVIASTA KTSERNLRQL ETQDGFGLTL LHVIASTNLP
60 70 80 90 100
LSTRLAGALF FKNFIKRKWV DENGNHLLPA NNVELIKKEI VPLMISLPNN
110 120 130 140 150
LQVQIGEAIS SIADSDFPDR WPTLLSDLAS RLSNDDMVTN KGVLTVAHSI
160 170 180 190 200
FKRWRPLFRS DELFLEIKLV LDVFTAPFLN LLKTVDEQIT ANENNKASLN
210 220 230 240 250
ILFDVLLVLI KLYYDFNCQD IPEFFEDNIQ VGMGIFHKYL SYSNPLLEDP
260 270 280 290 300
DETEHASVLI KVKSSIQELV QLYTTRYEDV FGPMINEFIQ ITWNLLTSIS
310 320 330 340 350
NQPKYDILVS KSLSFLTAVT RIPKYFEIFN NESAMNNITE QIILPNVTLR
360 370 380 390 400
EEDVELFEDD PIEYIRRDLE GSDTDTRRRA CTDFLKELKE KNEVLVTNIF
410 420 430 440 450
LAHMKGFVDQ YMSDPSKNWK FKDLYIYLFT ALAINGNITN AGVSSTNNLL
460 470 480 490 500
NVVDFFTKEI APDLTSNNIP HIILRVDAIK YIYTFRNQLT KAQLIELMPI
510 520 530 540 550
LATFLQTDEY VVYTYAAITI EKILTIRESN TSPAFIFHKE DISNSTEILL
560 570 580 590 600
KNLIALILKH GSSPEKLAEN EFLMRSIFRV LQTSEDSIQP LFPQLLAQFI
610 620 630 640 650
EIVTIMAKNP SNPRFTHYTF ESIGAILNYT QRQNLPLLVD SMMPTFLTVF
660 670 680 690 700
SEDIQEFIPY VFQIIAFVVE QSATIPESIK PLAQPLLAPN VWELKGNIPA
710 720 730 740 750
VTRLLKSFIK TDSSIFPDLV PVLGIFQRLI ASKAYEVHGF DLLEHIMLLI
760 770 780 790 800
DMNRLRPYIK QIAVLLLQRL QNSKTERYVK KLTVFFGLIS NKLGSDFLIH
810 820 830 840 850
FIDEVQDGLF QQIWGNFIIT TLPTIGNLLD RKIALIGVLN MVINGQFFQS
860 870 880 890 900
KYPTLISSTM NSIIETASSQ SIANLKNDYV DLDNLEEIST FGSHFSKLVS
910 920 930 940 950
ISEKPFDPLP EIDVNNGVRL YVAEALNKYN AISGNTFLNT ILPQLTQENQ
960
VKLNQLLVGN
Length:960
Mass (Da):109,356
Last modified:October 1, 1996 - v2
Checksum:i0A32D221DFC2C483
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti515 – 5151Y → D in AAA34531. (PubMed:8336709)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14838 Genomic DNA. Translation: AAA34531.1.
Z72761 Genomic DNA. Translation: CAA96957.1.
Z49149 Genomic DNA. Translation: CAA89018.1.
BK006941 Genomic DNA. Translation: DAA07880.1.
PIRiA48083.
RefSeqiNP_011276.1. NM_001181104.1.

Genome annotation databases

EnsemblFungiiYGL238W; YGL238W; YGL238W.
GeneIDi852612.
KEGGisce:YGL238W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14838 Genomic DNA. Translation: AAA34531.1 .
Z72761 Genomic DNA. Translation: CAA96957.1 .
Z49149 Genomic DNA. Translation: CAA89018.1 .
BK006941 Genomic DNA. Translation: DAA07880.1 .
PIRi A48083.
RefSeqi NP_011276.1. NM_001181104.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WA5 X-ray 2.00 C 1-960 [» ]
1Z3H X-ray 3.10 A/B 1-960 [» ]
ProteinModelPortali P33307.
SMRi P33307. Positions 1-958.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33001. 42 interactions.
DIPi DIP-1131N.
IntActi P33307. 22 interactions.
MINTi MINT-404965.
STRINGi 4932.YGL238W.

Proteomic databases

MaxQBi P33307.
PaxDbi P33307.
PeptideAtlasi P33307.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL238W ; YGL238W ; YGL238W .
GeneIDi 852612.
KEGGi sce:YGL238W.

Organism-specific databases

CYGDi YGL238w.
SGDi S000003207. CSE1.

Phylogenomic databases

eggNOGi COG5657.
GeneTreei ENSGT00550000074884.
HOGENOMi HOG000201317.
InParanoidi P33307.
KOi K18423.
OMAi QFVTAVW.
OrthoDBi EOG78WM14.

Enzyme and pathway databases

BioCyci YEAST:G3O-30711-MONOMER.

Miscellaneous databases

EvolutionaryTracei P33307.
NextBioi 971811.
PROi P33307.

Gene expression databases

Genevestigatori P33307.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR005043. CAS_CSE1_C.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view ]
Pfami PF03378. CAS_CSE1. 1 hit.
PF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view ]
SMARTi SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae."
    Xiao Z., McGrew J.T., Schroeder A.J., Fitzgerald-Hayes M.
    Mol. Cell. Biol. 13:4691-4702(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1519-1523(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
    Strain: ATCC 204508 / S288c.
  5. "Cse1p functions as the nuclear export receptor for importin alpha in yeast."
    Kunzler M., Hurt E.C.
    FEBS Lett. 433:185-190(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae."
    Hood J.K., Silver P.A.
    J. Biol. Chem. 273:35142-35146(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Cse1p is involved in export of yeast importin alpha from the nucleus."
    Solsbacher J., Maurer P., Bischoff F.R., Schlenstedt G.
    Mol. Cell. Biol. 18:6805-6815(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p."
    Schroeder A.J., Chen X.H., Xiao Z., Fitzgerald-Hayes M.
    Mol. Gen. Genet. 261:788-795(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Structural basis for the assembly of a nuclear export complex."
    Matsuura Y., Stewart M.
    Nature 432:872-877(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH SRP1 CARGO AND DOG RANGTP.

Entry informationi

Entry nameiCSE1_YEAST
AccessioniPrimary (citable) accession number: P33307
Secondary accession number(s): D6VV96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3