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Reviewed, UniProtKB/Swiss-Prot P33307 (CSE1_YEAST)

Last modified December 15, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Importin alpha re-exporter
Alternative name(s):
    Chromosome segregation protein CSE1
Gene names
Name: CSE1
Ordered Locus Names: YGL238W
ORF Names: HRC135
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length960 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Binds with high affinity to SRP1 only in the presence of RanGTP. The complex is dissociated by the RanGTP-binding protein YRB1.

Subcellular location

Cytoplasm. Nucleus.

Miscellaneous

Present with 23500 molecules/cell in log phase SD medium. Ref.8

Sequence similarities

Belongs to the XPO2/CSE1 family.

Contains 20 HEAT repeats.

Contains 1 importin N-terminal domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 960960Importin alpha re-exporter
PRO_0000117293

Regions

Repeat1 – 3333HEAT 1
Domain23 – 9674Importin N-terminal
Repeat34 – 7340HEAT 2
Repeat74 – 12047HEAT 3
Repeat121 – 15737HEAT 4
Repeat158 – 22063HEAT 5
Repeat221 – 27858HEAT 6
Repeat279 – 32345HEAT 7
Repeat324 – 39269HEAT 8
Repeat393 – 44553HEAT 9
Repeat446 – 48944HEAT 10
Repeat490 – 52839HEAT 11
Repeat529 – 58658HEAT 12
Repeat587 – 63044HEAT 13
Repeat631 – 67444HEAT 14
Repeat675 – 71642HEAT 15
Repeat717 – 75135HEAT 16
Repeat752 – 79443HEAT 17
Repeat795 – 82632HEAT 18
Repeat827 – 928102HEAT 19; with insert
Repeat929 – 96032HEAT 20
Motif366 – 38116Nuclear localization signal Potential

Amino acid modifications

Modified residue181Phosphoserine Ref.9

Experimental info

Sequence conflict5151Y → D in AAA34531. Ref.1

Secondary structure

1 ........................................................................................................................................... 960
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P33307-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 0A32D221DFC2C483

FASTA960109,356
        10         20         30         40         50         60 
MSDLETVAKF LAESVIASTA KTSERNLRQL ETQDGFGLTL LHVIASTNLP LSTRLAGALF 

        70         80         90        100        110        120 
FKNFIKRKWV DENGNHLLPA NNVELIKKEI VPLMISLPNN LQVQIGEAIS SIADSDFPDR 

       130        140        150        160        170        180 
WPTLLSDLAS RLSNDDMVTN KGVLTVAHSI FKRWRPLFRS DELFLEIKLV LDVFTAPFLN 

       190        200        210        220        230        240 
LLKTVDEQIT ANENNKASLN ILFDVLLVLI KLYYDFNCQD IPEFFEDNIQ VGMGIFHKYL 

       250        260        270        280        290        300 
SYSNPLLEDP DETEHASVLI KVKSSIQELV QLYTTRYEDV FGPMINEFIQ ITWNLLTSIS 

       310        320        330        340        350        360 
NQPKYDILVS KSLSFLTAVT RIPKYFEIFN NESAMNNITE QIILPNVTLR EEDVELFEDD 

       370        380        390        400        410        420 
PIEYIRRDLE GSDTDTRRRA CTDFLKELKE KNEVLVTNIF LAHMKGFVDQ YMSDPSKNWK 

       430        440        450        460        470        480 
FKDLYIYLFT ALAINGNITN AGVSSTNNLL NVVDFFTKEI APDLTSNNIP HIILRVDAIK 

       490        500        510        520        530        540 
YIYTFRNQLT KAQLIELMPI LATFLQTDEY VVYTYAAITI EKILTIRESN TSPAFIFHKE 

       550        560        570        580        590        600 
DISNSTEILL KNLIALILKH GSSPEKLAEN EFLMRSIFRV LQTSEDSIQP LFPQLLAQFI 

       610        620        630        640        650        660 
EIVTIMAKNP SNPRFTHYTF ESIGAILNYT QRQNLPLLVD SMMPTFLTVF SEDIQEFIPY 

       670        680        690        700        710        720 
VFQIIAFVVE QSATIPESIK PLAQPLLAPN VWELKGNIPA VTRLLKSFIK TDSSIFPDLV 

       730        740        750        760        770        780 
PVLGIFQRLI ASKAYEVHGF DLLEHIMLLI DMNRLRPYIK QIAVLLLQRL QNSKTERYVK 

       790        800        810        820        830        840 
KLTVFFGLIS NKLGSDFLIH FIDEVQDGLF QQIWGNFIIT TLPTIGNLLD RKIALIGVLN 

       850        860        870        880        890        900 
MVINGQFFQS KYPTLISSTM NSIIETASSQ SIANLKNDYV DLDNLEEIST FGSHFSKLVS 

       910        920        930        940        950        960 
ISEKPFDPLP EIDVNNGVRL YVAEALNKYN AISGNTFLNT ILPQLTQENQ VKLNQLLVGN

« Hide

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References

« Hide 'large scale' references
[1]"CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae."
Xiao Z., McGrew J.T., Schroeder A.J., Fitzgerald-Hayes M.
Mol. Cell. Biol. 13:4691-4702(1993) [PubMed: 8336709] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
Vandenbol M., Durand P., Portetelle D., Hilger F.
Yeast 11:1519-1523(1995) [PubMed: 8750240] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
Strain: ATCC 204508 / S288c.
[4]"Cse1p functions as the nuclear export receptor for importin alpha in yeast."
Kunzler M., Hurt E.C.
FEBS Lett. 433:185-190(1998) [PubMed: 9744791] [Abstract]
Cited for: FUNCTION.
[5]"Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae."
Hood J.K., Silver P.A.
J. Biol. Chem. 273:35142-35146(1998) [PubMed: 9857050] [Abstract]
Cited for: FUNCTION.
[6]"Cse1p is involved in export of yeast importin alpha from the nucleus."
Solsbacher J., Maurer P., Bischoff F.R., Schlenstedt G.
Mol. Cell. Biol. 18:6805-6815(1998) [PubMed: 9774694] [Abstract]
Cited for: FUNCTION.
[7]"Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p."
Schroeder A.J., Chen X.H., Xiao Z., Fitzgerald-Hayes M.
Mol. Gen. Genet. 261:788-795(1999) [PubMed: 10394916] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY.
[10]"Structural basis for the assembly of a nuclear export complex."
Matsuura Y., Stewart M.
Nature 432:872-877(2004) [PubMed: 15602554] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH SRP1 CARGO AND DOG RANGTP.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L14838 Genomic DNA. Translation: AAA34531.1.
Z72761 Genomic DNA. Translation: CAA96957.1.
Z49149 Genomic DNA. Translation: CAA89018.1.
PIRA48083.
RefSeqNP_011276.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1WA5X-ray2.00C1-960[»]
1Z3HX-ray3.10A/B1-960[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1131N.
IntActP33307. 23 interactions.
STRINGP33307.

Proteomic databases

PeptideAtlasP33307.

Genome annotation databases

EnsemblYGL238W; YGL238W; YGL238W; Saccharomyces cerevisiae. [Genome view]
GeneID852612.
KEGGsce:YGL238W.
NMPDRfig|4932.3.peg.2379.

Organism-specific databases

CYGDYGL238w.
SGDS000003207. CSE1.

Phylogenomic databases

HOGENOMHBG327833.
OMAGNIPPLV.
OrthoDBEOG9HB031.

Gene expression databases

ArrayExpressP33307.
GenevestigatorP33307.
GermOnlineYGL238W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR005043. CAS_CSE1_C.
IPR013713. Exportin_Cse1-like.
IPR001494. Importin-b_N.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 1 hit.
PfamPF03378. CAS_CSE1. 1 hit.
PF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
PROSITEPS50077. HEAT_REPEAT. False negative.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio971811.

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Entry information

Entry nameCSE1_YEAST
AccessionPrimary (citable) accession number: P33307
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: December 15, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents