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P33307

- CSE1_YEAST

UniProt

P33307 - CSE1_YEAST

Protein

Importin alpha re-exporter

Gene

CSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.4 Publications

    GO - Molecular functioni

    1. importin-alpha export receptor activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein export from nucleus Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30711-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Importin alpha re-exporter
    Alternative name(s):
    Chromosome segregation protein CSE1
    Gene namesi
    Name:CSE1
    Ordered Locus Names:YGL238W
    ORF Names:HRC135
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL238w.
    SGDiS000003207. CSE1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear envelope Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 960960Importin alpha re-exporterPRO_0000117293Add
    BLAST

    Proteomic databases

    MaxQBiP33307.
    PaxDbiP33307.
    PeptideAtlasiP33307.

    Expressioni

    Gene expression databases

    GenevestigatoriP33307.

    Interactioni

    Subunit structurei

    Binds with high affinity to SRP1 only in the presence of RanGTP. The complex is dissociated by the RanGTP-binding protein YRB1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SRP1Q028212EBI-5168,EBI-1797

    Protein-protein interaction databases

    BioGridi33001. 42 interactions.
    DIPiDIP-1131N.
    IntActiP33307. 22 interactions.
    MINTiMINT-404965.
    STRINGi4932.YGL238W.

    Structurei

    Secondary structure

    1
    960
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1311
    Helixi17 – 193
    Helixi20 – 3112
    Helixi36 – 4510
    Helixi51 – 6818
    Beta strandi72 – 743
    Beta strandi76 – 783
    Helixi80 – 9617
    Helixi99 – 11618
    Turni118 – 1203
    Helixi124 – 1296
    Helixi139 – 15113
    Helixi152 – 1543
    Helixi161 – 18828
    Helixi197 – 21721
    Helixi223 – 2275
    Helixi229 – 24012
    Helixi245 – 2473
    Beta strandi253 – 2553
    Helixi258 – 27619
    Helixi278 – 29821
    Helixi303 – 3053
    Helixi306 – 32015
    Helixi323 – 3264
    Helixi327 – 3293
    Helixi332 – 34110
    Helixi343 – 3475
    Helixi351 – 3533
    Turni354 – 3596
    Helixi361 – 37010
    Helixi377 – 39115
    Helixi393 – 41220
    Turni415 – 4173
    Helixi419 – 43315
    Beta strandi434 – 4363
    Helixi452 – 4598
    Helixi461 – 4655
    Helixi472 – 48413
    Helixi486 – 4883
    Helixi491 – 50414
    Helixi510 – 52314
    Beta strandi530 – 5323
    Beta strandi535 – 5373
    Helixi539 – 5413
    Turni542 – 5454
    Helixi546 – 55813
    Helixi564 – 5674
    Helixi571 – 58414
    Turni585 – 5884
    Helixi589 – 5913
    Helixi592 – 60615
    Helixi613 – 62816
    Helixi632 – 6343
    Helixi635 – 65117
    Turni655 – 6573
    Helixi658 – 67114
    Beta strandi672 – 6743
    Turni677 – 6793
    Helixi680 – 6823
    Helixi683 – 6864
    Helixi689 – 6924
    Turni695 – 6973
    Helixi698 – 71215
    Helixi713 – 7153
    Helixi720 – 73011
    Turni733 – 7353
    Helixi736 – 74914
    Helixi752 – 7554
    Helixi756 – 7583
    Helixi759 – 76810
    Helixi769 – 7713
    Helixi776 – 79318
    Helixi795 – 8039
    Helixi809 – 8168
    Helixi818 – 8214
    Helixi822 – 8243
    Helixi828 – 84316
    Helixi846 – 8516
    Helixi853 – 8553
    Helixi856 – 86813
    Helixi894 – 8963
    Helixi899 – 9013
    Turni914 – 9163
    Helixi917 – 93216
    Beta strandi934 – 9363
    Helixi937 – 9415
    Helixi942 – 9443
    Helixi947 – 95711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WA5X-ray2.00C1-960[»]
    1Z3HX-ray3.10A/B1-960[»]
    ProteinModelPortaliP33307.
    SMRiP33307. Positions 1-958.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33307.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 3333HEAT 1Add
    BLAST
    Domaini23 – 9674Importin N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Repeati34 – 7340HEAT 2Add
    BLAST
    Repeati74 – 12047HEAT 3Add
    BLAST
    Repeati121 – 15737HEAT 4Add
    BLAST
    Repeati158 – 22063HEAT 5Add
    BLAST
    Repeati221 – 27858HEAT 6Add
    BLAST
    Repeati279 – 32345HEAT 7Add
    BLAST
    Repeati324 – 39269HEAT 8Add
    BLAST
    Repeati393 – 44553HEAT 9Add
    BLAST
    Repeati446 – 48944HEAT 10Add
    BLAST
    Repeati490 – 52839HEAT 11Add
    BLAST
    Repeati529 – 58658HEAT 12Add
    BLAST
    Repeati587 – 63044HEAT 13Add
    BLAST
    Repeati631 – 67444HEAT 14Add
    BLAST
    Repeati675 – 71642HEAT 15Add
    BLAST
    Repeati717 – 75135HEAT 16Add
    BLAST
    Repeati752 – 79443HEAT 17Add
    BLAST
    Repeati795 – 82632HEAT 18Add
    BLAST
    Repeati827 – 928102HEAT 19; with insertAdd
    BLAST
    Repeati929 – 96032HEAT 20Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi366 – 38116Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the XPO2/CSE1 family.Curated
    Contains 20 HEAT repeats.Curated
    Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5657.
    GeneTreeiENSGT00550000074884.
    HOGENOMiHOG000201317.
    OMAiQFVTAVW.
    OrthoDBiEOG78WM14.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR005043. CAS_CSE1_C.
    IPR013713. Cse1.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF03378. CAS_CSE1. 1 hit.
    PF08506. Cse1. 1 hit.
    PF03810. IBN_N. 1 hit.
    [Graphical view]
    SMARTiSM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33307-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDLETVAKF LAESVIASTA KTSERNLRQL ETQDGFGLTL LHVIASTNLP    50
    LSTRLAGALF FKNFIKRKWV DENGNHLLPA NNVELIKKEI VPLMISLPNN 100
    LQVQIGEAIS SIADSDFPDR WPTLLSDLAS RLSNDDMVTN KGVLTVAHSI 150
    FKRWRPLFRS DELFLEIKLV LDVFTAPFLN LLKTVDEQIT ANENNKASLN 200
    ILFDVLLVLI KLYYDFNCQD IPEFFEDNIQ VGMGIFHKYL SYSNPLLEDP 250
    DETEHASVLI KVKSSIQELV QLYTTRYEDV FGPMINEFIQ ITWNLLTSIS 300
    NQPKYDILVS KSLSFLTAVT RIPKYFEIFN NESAMNNITE QIILPNVTLR 350
    EEDVELFEDD PIEYIRRDLE GSDTDTRRRA CTDFLKELKE KNEVLVTNIF 400
    LAHMKGFVDQ YMSDPSKNWK FKDLYIYLFT ALAINGNITN AGVSSTNNLL 450
    NVVDFFTKEI APDLTSNNIP HIILRVDAIK YIYTFRNQLT KAQLIELMPI 500
    LATFLQTDEY VVYTYAAITI EKILTIRESN TSPAFIFHKE DISNSTEILL 550
    KNLIALILKH GSSPEKLAEN EFLMRSIFRV LQTSEDSIQP LFPQLLAQFI 600
    EIVTIMAKNP SNPRFTHYTF ESIGAILNYT QRQNLPLLVD SMMPTFLTVF 650
    SEDIQEFIPY VFQIIAFVVE QSATIPESIK PLAQPLLAPN VWELKGNIPA 700
    VTRLLKSFIK TDSSIFPDLV PVLGIFQRLI ASKAYEVHGF DLLEHIMLLI 750
    DMNRLRPYIK QIAVLLLQRL QNSKTERYVK KLTVFFGLIS NKLGSDFLIH 800
    FIDEVQDGLF QQIWGNFIIT TLPTIGNLLD RKIALIGVLN MVINGQFFQS 850
    KYPTLISSTM NSIIETASSQ SIANLKNDYV DLDNLEEIST FGSHFSKLVS 900
    ISEKPFDPLP EIDVNNGVRL YVAEALNKYN AISGNTFLNT ILPQLTQENQ 950
    VKLNQLLVGN 960
    Length:960
    Mass (Da):109,356
    Last modified:October 1, 1996 - v2
    Checksum:i0A32D221DFC2C483
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti515 – 5151Y → D in AAA34531. (PubMed:8336709)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14838 Genomic DNA. Translation: AAA34531.1.
    Z72761 Genomic DNA. Translation: CAA96957.1.
    Z49149 Genomic DNA. Translation: CAA89018.1.
    BK006941 Genomic DNA. Translation: DAA07880.1.
    PIRiA48083.
    RefSeqiNP_011276.1. NM_001181104.1.

    Genome annotation databases

    EnsemblFungiiYGL238W; YGL238W; YGL238W.
    GeneIDi852612.
    KEGGisce:YGL238W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14838 Genomic DNA. Translation: AAA34531.1 .
    Z72761 Genomic DNA. Translation: CAA96957.1 .
    Z49149 Genomic DNA. Translation: CAA89018.1 .
    BK006941 Genomic DNA. Translation: DAA07880.1 .
    PIRi A48083.
    RefSeqi NP_011276.1. NM_001181104.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WA5 X-ray 2.00 C 1-960 [» ]
    1Z3H X-ray 3.10 A/B 1-960 [» ]
    ProteinModelPortali P33307.
    SMRi P33307. Positions 1-958.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33001. 42 interactions.
    DIPi DIP-1131N.
    IntActi P33307. 22 interactions.
    MINTi MINT-404965.
    STRINGi 4932.YGL238W.

    Proteomic databases

    MaxQBi P33307.
    PaxDbi P33307.
    PeptideAtlasi P33307.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL238W ; YGL238W ; YGL238W .
    GeneIDi 852612.
    KEGGi sce:YGL238W.

    Organism-specific databases

    CYGDi YGL238w.
    SGDi S000003207. CSE1.

    Phylogenomic databases

    eggNOGi COG5657.
    GeneTreei ENSGT00550000074884.
    HOGENOMi HOG000201317.
    OMAi QFVTAVW.
    OrthoDBi EOG78WM14.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30711-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P33307.
    NextBioi 971811.
    PROi P33307.

    Gene expression databases

    Genevestigatori P33307.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR005043. CAS_CSE1_C.
    IPR013713. Cse1.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF03378. CAS_CSE1. 1 hit.
    PF08506. Cse1. 1 hit.
    PF03810. IBN_N. 1 hit.
    [Graphical view ]
    SMARTi SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae."
      Xiao Z., McGrew J.T., Schroeder A.J., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 13:4691-4702(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
      Vandenbol M., Durand P., Portetelle D., Hilger F.
      Yeast 11:1519-1523(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
      Strain: ATCC 204508 / S288c.
    5. "Cse1p functions as the nuclear export receptor for importin alpha in yeast."
      Kunzler M., Hurt E.C.
      FEBS Lett. 433:185-190(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae."
      Hood J.K., Silver P.A.
      J. Biol. Chem. 273:35142-35146(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Cse1p is involved in export of yeast importin alpha from the nucleus."
      Solsbacher J., Maurer P., Bischoff F.R., Schlenstedt G.
      Mol. Cell. Biol. 18:6805-6815(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p."
      Schroeder A.J., Chen X.H., Xiao Z., Fitzgerald-Hayes M.
      Mol. Gen. Genet. 261:788-795(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Structural basis for the assembly of a nuclear export complex."
      Matsuura Y., Stewart M.
      Nature 432:872-877(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH SRP1 CARGO AND DOG RANGTP.

    Entry informationi

    Entry nameiCSE1_YEAST
    AccessioniPrimary (citable) accession number: P33307
    Secondary accession number(s): D6VV96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 23500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3