P33306 (BCK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BCK2

Alternative name(s):
Bypass of kinase C protein

Gene names

Name:	BCK2
Synonyms:	CTR7
Ordered Locus Names:	YER167W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Dosage dependent suppressor of PKC1 deletion and MPK1 deletion. Involved in cell lysis.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from genetic interaction PubMed 12518319. Source: SGD positive regulation of gene expression Inferred from mutant phenotype PubMed 19158491. Source: SGD regulation of cell cycle Inferred from mutant phenotype PubMed 7891677. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FUS3	P16892	2	EBI-3480,EBI-7193
KEL1	P38853	2	EBI-3480,EBI-9619
KIN2	P13186	4	EBI-3480,EBI-9723
KSS1	P14681	5	EBI-3480,EBI-9945

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	Protein BCK2		PRO_0000064883
Amino acid modifications
	Modified residue	317	1	Phosphoserine Ref.5
	Modified residue	334	1	Phosphoserine Ref.4
	Modified residue	757	1	Phosphoserine Ref.5
	Modified residue	761	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	4 – 5	2	NS → HC in AAA34452. Ref.1
	Sequence conflict	100	1	T → A in AAA34452. Ref.1
	Sequence conflict	167	1	L → F in AAA34452. Ref.1
	Sequence conflict	812	1	F → C in AAA34452. Ref.1
	Sequence conflict	827	1	N → K in AAA34452. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33306 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 9F824F2701FC452D	FASTA	851	93,738
	10 20 30 40 50 60 MPKNSHHHRS SSVNSTKSRS TESTNKWKIP HYYRRSASGS TQASPDRNSS TGSCSTPVLP 70 80 90 100 110 120 TMNVMSSPKK VLLEDPRDNH TKAKKSSRKK SGEMVFVNYT VQDTANENDT DLQTQPVSVP 130 140 150 160 170 180 APKAKLKKKS SKRRMLKIFG SSKNEHIEDI VEEQPMVLQM DSESKPLSGT PISESGIDAS 190 200 210 220 230 240 SLTTKRSYNS FLKHNRLNGK TPFSGNLSFP SLNMMGNTTD LPIDNNDFCS EKEVVPKSTH 250 260 270 280 290 300 DPSLAKPPSR FTESETNSTP NLSSIPLMNT KNTRLKYNKV APQSSDRQKS QESGLYHSTE 310 320 330 340 350 360 SFNFKDQNYS NNKSSLSLNS DLSTPHFAKH SPDSPRTSRS FNCGDSQSKV KLPEENDASI 370 380 390 400 410 420 AFSKMFTRKR ANTGGSTCSL ASPTIAQTIQ QSNIKVNKLP TQRTTSVGSL SSMSNRYSPI 430 440 450 460 470 480 RVASPGRARS ATRGSSLYRL SRDLNSLPSV TDLPEMDSTT PVNEIFLDGQ PQHKSGSVKG 490 500 510 520 530 540 GHRKKQESIS DAQRIQHSNS YITTPSSSLV TPPYYMTGYT LPSSASASST PNVLETHNMN 550 560 570 580 590 600 FVPSTSTVTS YRPSSNFSSF DKEYSNENDA SGEFSAFNTP MENIPALKGI PRSTLEENEE 610 620 630 640 650 660 EDVLVQDIPN TAHFQRRDIM GMDTHRKDDS LDFNSLMPHG STTSSSIVDS VMTNSISTTT 670 680 690 700 710 720 SNATGNYFQD QDKYTLVNTG LGLSDANLDH FIRSQWKHAS RSESNNNTGN RVSYSGSTPN 730 740 750 760 770 780 NVDTTKTNLQ VYTEFDFENP ESFFHEQSKL LGEMGHSNNN SNSAINMNEP KSADTYIGNI 790 800 810 820 830 840 SPDTSATVSL GDLMGSNVSN NSERNFYDGH TFVPQYQANS SVENSNNQNA APIANNDIDN 850 NLQSFYFDNS N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A pair of functionally redundant yeast genes (PPZ1 and PPZ2) encoding type 1-related protein phosphatases function within the PKC1-mediated pathway." Lee K.S., Hines L.K., Levin D.E. Mol. Cell. Biol. 13:5843-5853(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-334, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317; SER-757 AND SER-761, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10242 Genomic DNA. Translation: AAA34452.1. U18922 Genomic DNA. Translation: AAB64694.1. BK006939 Genomic DNA. Translation: DAA07829.1.
PIR	S50670.
RefSeq	NP_011094.3. NM_001179057.3.
3D structure databases
ProteinModelPortal	P33306.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6406N.
IntAct	P33306. 13 interactions.
MINT	MINT-4484608.
STRING	4932.YER167W.
Proteomic databases
PaxDb	P33306.
PeptideAtlas	P33306.
PRIDE	P33306.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER167W; YER167W; YER167W.
GeneID	856914.
KEGG	sce:YER167W.
Organism-specific databases
CYGD	YER167w.
SGD	S000000969. BCK2.
Phylogenomic databases
eggNOG	NOG44363.
OMA	RPRSNTR.
OrthoDB	EOG4M3DK6.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30328-MONOMER.
Gene expression databases
Genevestigator	P33306.
GermOnline	YER167W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	983362.

Entry information

Entry name

BCK2_YEAST

Accession

Primary (citable) accession number: P33306
Secondary accession number(s): D3DM75

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program