ID   AFR1_YEAST              Reviewed;         620 AA.
AC   P33304; D6VS72;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   27-MAR-2024, entry version 156.
DE   RecName: Full=Protein AFR1;
GN   Name=AFR1; OrderedLocusNames=YDR085C; ORFNames=D4471;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8413281; DOI=10.1128/mcb.13.11.6876-6888.1993;
RA   Konopka J.B.;
RT   "AFR1 acts in conjunction with the alpha-factor receptor to promote
RT   morphogenesis and adaptation.";
RL   Mol. Cell. Biol. 13:6876-6888(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472 AND SER-526, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Acts in conjunction with the alpha-factor receptor to promote
CC       morphogenesis and adaptation.
CC   -!- INDUCTION: By pheromone (alpha-factor).
CC   -!- SIMILARITY: To yeast YER158C. {ECO:0000305}.
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DR   EMBL; Z46796; CAA86807.1; -; Genomic_DNA.
DR   EMBL; L21702; AAC37365.1; -; Unassigned_DNA.
DR   EMBL; X82086; CAA57614.1; -; Genomic_DNA.
DR   EMBL; Z74381; CAA98905.1; -; Genomic_DNA.
DR   EMBL; AY723775; AAU09692.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11932.1; -; Genomic_DNA.
DR   PIR; S48772; S48772.
DR   RefSeq; NP_010370.1; NM_001180393.1.
DR   AlphaFoldDB; P33304; -.
DR   BioGRID; 32141; 46.
DR   DIP; DIP-824N; -.
DR   IntAct; P33304; 7.
DR   MINT; P33304; -.
DR   STRING; 4932.YDR085C; -.
DR   iPTMnet; P33304; -.
DR   PaxDb; 4932-YDR085C; -.
DR   PeptideAtlas; P33304; -.
DR   EnsemblFungi; YDR085C_mRNA; YDR085C; YDR085C.
DR   GeneID; 851658; -.
DR   KEGG; sce:YDR085C; -.
DR   AGR; SGD:S000002492; -.
DR   SGD; S000002492; AFR1.
DR   VEuPathDB; FungiDB:YDR085C; -.
DR   eggNOG; ENOG502S3XF; Eukaryota.
DR   HOGENOM; CLU_440893_0_0_1; -.
DR   InParanoid; P33304; -.
DR   OMA; ESARYFY; -.
DR   OrthoDB; 2015099at2759; -.
DR   BioCyc; YEAST:G3O-29690-MONOMER; -.
DR   BioGRID-ORCS; 851658; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P33304; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P33304; Protein.
DR   GO; GO:0001400; C:mating projection base; IDA:SGD.
DR   GO; GO:0003779; F:actin binding; IBA:GO_Central.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0000753; P:cell morphogenesis involved in conjugation with cellular fusion; IMP:SGD.
DR   GO; GO:0097271; P:protein localization to bud neck; IMP:SGD.
DR   GO; GO:0032185; P:septin cytoskeleton organization; IMP:SGD.
DR   PANTHER; PTHR12751; PHOSPHATASE AND ACTIN REGULATOR PHACTR; 1.
DR   PANTHER; PTHR12751:SF20; PROTEIN AFR1; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..620
FT                   /note="Protein AFR1"
FT                   /id="PRO_0000064485"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         472
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         526
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
SQ   SEQUENCE   620 AA;  71364 MW;  A9ABFBC9A26E62E5 CRC64;
     MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN PNKKRHIYSG
     EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY HEGFNNDQDY VSQYQKPQYT
     FGVYKELTPY QLQRSKMKRS FQFPNGEIYK PKLDGKCTHS LKKPELNSRD SSLFKFSEKK
     GRNLSKDFVG PHNGTSVIHI PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS
     LPESQTDDDD GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK
     TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID DMVTHSDGNS
     EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND KYDLRRRQLE IRQKLHETSH
     NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS KLGEYIIDTR NQPPPRSSKR PSLDDNESAR
     YFYNISTDLR QSLSGPISLP MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK
     PIKKDVRFAK EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS
     YKKNEMVVHE NSKCFTHYLI
//