Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein AFR1

Gene

AFR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation.

GO - Molecular functioni

  1. receptor signaling protein activity Source: SGD

GO - Biological processi

  1. cell morphogenesis involved in conjugation Source: SGD
  2. cell morphogenesis involved in conjugation with cellular fusion Source: SGD
  3. intracellular signal transduction Source: GOC
  4. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  5. regulation of G-protein coupled receptor protein signaling pathway Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AFR1
Gene namesi
Name:AFR1
Ordered Locus Names:YDR085C
ORF Names:D4471
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR085c.
EuPathDBiFungiDB:YDR085C.
SGDiS000002492. AFR1.

Subcellular locationi

GO - Cellular componenti

  1. mating projection base Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 620620Protein AFR1PRO_0000064485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei472 – 4721Phosphoserine1 Publication
Modified residuei526 – 5261Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP33304.

Expressioni

Inductioni

By pheromone (alpha-factor).

Gene expression databases

GenevestigatoriP33304.

Interactioni

Protein-protein interaction databases

BioGridi32141. 19 interactions.
DIPiDIP-824N.
IntActiP33304. 6 interactions.
MINTiMINT-599820.
STRINGi4932.YDR085C.

Structurei

3D structure databases

ProteinModelPortaliP33304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast YER158C.Curated

Phylogenomic databases

eggNOGiNOG43797.
InParanoidiP33304.
OrthoDBiEOG7WT490.

Sequencei

Sequence statusi: Complete.

P33304-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN
60 70 80 90 100
PNKKRHIYSG EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY
110 120 130 140 150
HEGFNNDQDY VSQYQKPQYT FGVYKELTPY QLQRSKMKRS FQFPNGEIYK
160 170 180 190 200
PKLDGKCTHS LKKPELNSRD SSLFKFSEKK GRNLSKDFVG PHNGTSVIHI
210 220 230 240 250
PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS LPESQTDDDD
260 270 280 290 300
GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK
310 320 330 340 350
TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID
360 370 380 390 400
DMVTHSDGNS EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND
410 420 430 440 450
KYDLRRRQLE IRQKLHETSH NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS
460 470 480 490 500
KLGEYIIDTR NQPPPRSSKR PSLDDNESAR YFYNISTDLR QSLSGPISLP
510 520 530 540 550
MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK PIKKDVRFAK
560 570 580 590 600
EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS
610 620
YKKNEMVVHE NSKCFTHYLI
Length:620
Mass (Da):71,364
Last modified:February 1, 1994 - v1
Checksum:iA9ABFBC9A26E62E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46796 Genomic DNA. Translation: CAA86807.1.
L21702 Unassigned DNA. Translation: AAC37365.1.
X82086 Genomic DNA. Translation: CAA57614.1.
Z74381 Genomic DNA. Translation: CAA98905.1.
AY723775 Genomic DNA. Translation: AAU09692.1.
BK006938 Genomic DNA. Translation: DAA11932.1.
PIRiS48772.
RefSeqiNP_010370.1. NM_001180393.1.

Genome annotation databases

EnsemblFungiiYDR085C; YDR085C; YDR085C.
GeneIDi851658.
KEGGisce:YDR085C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46796 Genomic DNA. Translation: CAA86807.1.
L21702 Unassigned DNA. Translation: AAC37365.1.
X82086 Genomic DNA. Translation: CAA57614.1.
Z74381 Genomic DNA. Translation: CAA98905.1.
AY723775 Genomic DNA. Translation: AAU09692.1.
BK006938 Genomic DNA. Translation: DAA11932.1.
PIRiS48772.
RefSeqiNP_010370.1. NM_001180393.1.

3D structure databases

ProteinModelPortaliP33304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32141. 19 interactions.
DIPiDIP-824N.
IntActiP33304. 6 interactions.
MINTiMINT-599820.
STRINGi4932.YDR085C.

Proteomic databases

PaxDbiP33304.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR085C; YDR085C; YDR085C.
GeneIDi851658.
KEGGisce:YDR085C.

Organism-specific databases

CYGDiYDR085c.
EuPathDBiFungiDB:YDR085C.
SGDiS000002492. AFR1.

Phylogenomic databases

eggNOGiNOG43797.
InParanoidiP33304.
OrthoDBiEOG7WT490.

Enzyme and pathway databases

BioCyciYEAST:G3O-29690-MONOMER.

Miscellaneous databases

NextBioi969260.
PROiP33304.

Gene expression databases

GenevestigatoriP33304.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation."
    Konopka J.B.
    Mol. Cell. Biol. 13:6876-6888(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472 AND SER-526, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAFR1_YEAST
AccessioniPrimary (citable) accession number: P33304
Secondary accession number(s): D6VS72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 29, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.