Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinate/fumarate mitochondrial transporter

Gene

SFC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transports cytoplasmic succinate, derived from isocitrate by the action of isocitrate lyase in the cytosol, into the mitochondrial matrix in exchange for fumarate.

GO - Molecular functioni

  • succinate:fumarate antiporter activity Source: SGD

GO - Biological processi

  • fumarate transport Source: SGD
  • succinate transmembrane transport Source: GOC
  • succinate transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31723-MONOMER.

Protein family/group databases

TCDBi2.A.29.13.1. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate/fumarate mitochondrial transporter
Alternative name(s):
Regulator of acetyl-CoA synthase activity
Gene namesi
Name:SFC1
Synonyms:ACR1
Ordered Locus Names:YJR095W
ORF Names:J1921
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR095W.
SGDiS000003856. SFC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei68 – 8821Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei114 – 13421Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei177 – 19317Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei219 – 23517Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei278 – 29518Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Succinate/fumarate mitochondrial transporterPRO_0000090679Add
BLAST

Proteomic databases

MaxQBiP33303.
PeptideAtlasiP33303.
TopDownProteomicsiP33303.

Expressioni

Inductioni

By ethanol or acetate as sole carbon sources. Repressed by glucose.

Interactioni

Protein-protein interaction databases

BioGridi33850. 30 interactions.
DIPiDIP-8019N.
IntActiP33303. 2 interactions.
MINTiMINT-1354606.

Structurei

3D structure databases

ProteinModelPortaliP33303.
SMRiP33303. Positions 12-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati8 – 9992Solcar 1Add
BLAST
Repeati111 – 20292Solcar 2Add
BLAST
Repeati212 – 30392Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000130868.
HOGENOMiHOG000168307.
InParanoidiP33303.
KOiK15100.
OMAiEMFRNEG.
OrthoDBiEOG7Q5HQD.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKKKASHP AINLMAGGTA GLFEALCCHP LDTIKVRMQI YRRVAGIEHV
60 70 80 90 100
KPPGFIKTGR TIYQKEGFLA LYKGLGAVVI GIIPKMAIRF SSYEFYRTLL
110 120 130 140 150
VNKESGIVST GNTFVAGVGA GITEAVLVVN PMEVVKIRLQ AQHLTPSEPN
160 170 180 190 200
AGPKYNNAIH AAYTIVKEEG VSALYRGVSL TAARQATNQG ANFTVYSKLK
210 220 230 240 250
EFLQNYHQMD VLPSWETSCI GLISGAIGPF SNAPLDTIKT RLQKDKSISL
260 270 280 290 300
EKQSGMKKII TIGAQLLKEE GFRALYKGIT PRVMRVAPGQ AVTFTVYEYV
310 320
REHLENLGIF KKNDTPKPKP LK
Length:322
Mass (Da):35,340
Last modified:November 1, 1995 - v2
Checksum:iEC29718A0F5011A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti300 – 32223VREHL…PKPLK → RKGAFQKIWVYSRRMTHQSQ SH in CAA80973 (PubMed:7908717).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25485 Genomic DNA. Translation: CAA80973.1.
Z49595 Genomic DNA. Translation: CAA89624.1.
AY692924 Genomic DNA. Translation: AAT92943.1.
BK006943 Genomic DNA. Translation: DAA08880.1.
PIRiS57116.
RefSeqiNP_012629.1. NM_001181753.1.

Genome annotation databases

EnsemblFungiiYJR095W; YJR095W; YJR095W.
GeneIDi853558.
KEGGisce:YJR095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25485 Genomic DNA. Translation: CAA80973.1.
Z49595 Genomic DNA. Translation: CAA89624.1.
AY692924 Genomic DNA. Translation: AAT92943.1.
BK006943 Genomic DNA. Translation: DAA08880.1.
PIRiS57116.
RefSeqiNP_012629.1. NM_001181753.1.

3D structure databases

ProteinModelPortaliP33303.
SMRiP33303. Positions 12-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33850. 30 interactions.
DIPiDIP-8019N.
IntActiP33303. 2 interactions.
MINTiMINT-1354606.

Protein family/group databases

TCDBi2.A.29.13.1. the mitochondrial carrier (mc) family.

Proteomic databases

MaxQBiP33303.
PeptideAtlasiP33303.
TopDownProteomicsiP33303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR095W; YJR095W; YJR095W.
GeneIDi853558.
KEGGisce:YJR095W.

Organism-specific databases

EuPathDBiFungiDB:YJR095W.
SGDiS000003856. SFC1.

Phylogenomic databases

GeneTreeiENSGT00840000130868.
HOGENOMiHOG000168307.
InParanoidiP33303.
KOiK15100.
OMAiEMFRNEG.
OrthoDBiEOG7Q5HQD.

Enzyme and pathway databases

BioCyciYEAST:G3O-31723-MONOMER.

Miscellaneous databases

PROiP33303.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ACR1, a gene encoding a protein related to mitochondrial carriers, is essential for acetyl-CoA synthetase activity in Saccharomyces cerevisiae."
    Fernandez M., Fernandez E., Rodicio R.
    Mol. Gen. Genet. 242:727-735(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification of the yeast ACR1 gene product as a succinate-fumarate transporter essential for growth on ethanol or acetate."
    Palmieri L., Lasorsa F.M., De Palma A., Palmieri F., Runswick M.J., Walker J.E.
    FEBS Lett. 417:114-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiSFC1_YEAST
AccessioniPrimary (citable) accession number: P33303
Secondary accession number(s): D6VWR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.