Pleiotropic ABC efflux transporter of multiple drugs - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P33302 (PDR5_YEAST)

Last modified July 7, 2009. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Pleiotropic ABC efflux transporter of multiple drugs
Alternative name(s):
    Pleiotropic drug resistance protein 5
    Suppressor of toxicity of sporidesmin

Gene names

Name:	PDR5
Synonyms:	LEM1, STS1, YDR1
Ordered Locus Names:	YOR153W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	1511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Active efflux of weakly charged organic compounds of 90 cubic Angstroms to 300 cubic Angstroms surface volume. Confers resistance to numerous chemicals including cycloheximide, sulfomethuron methyl, steroids, antiseptics, antibiotics, anticancer, herbicides, mycotoxins, insecticides, ionophores, alkaloids, flavonoids, phenothiazines, organotin compounds, carbazoles, lysosomotropic aminoesters, detergents, rhodamines and other fluorophores, azoles and other antifungals. Exhibits nucleoside triphosphatase activity. Ref.13 Ref.16 Ref.18 Ref.19 Ref.26 Ref.27 Ref.33 Ref.39 Ref.44 Ref.45 Ref.56 Ref.62 Ref.68 Ref.70
Enzyme regulation	FK506, isonitrile, enniatin, RU49953, kitasatospora E420, staurosporine CGP42700, prenyl-flavonoids, D-octapeptides were found to be inhibitors in vivo. Vanadate and oligomycin were found to be inhibitors in vitro.
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: The ERAD mutants 'Pro-183' and 'Tyr-1427' fail to reach the plasma membrane. The mutant 'Pro-183' accumulates into ER-associated compartments. Ref.43
Induction	Expressed during exponential growth. Transcription is transiently activated within 40 min after induction by benomyl and other toxic chemicals. Multidrug resistance and PDR5 mRNA level are activated by the transcription regulators PDR1, PDR3, YAP1, YAP2, STB5 and by the mitochondrial rho zero mutation. Mutations or deletion in the PDR1 or PDR3 transcription factors strongly activate PDR5 mRNA and PDR5 translation. The transcription regulator RDR1 represses PDR5 expression. Ref.22 Ref.57 Ref.63 Ref.65 Ref.66 Ref.67
Domain	The N-terminal ABC transporter domain (positions 161 to 410) contains degenerated Walker A and B ATP-binding motifs, suggesting that it may be less efficient in ATP binding or not functional at all. This is a distinctive feature of the PDR subfamily. Ref.2 The unusual length of the two extracellular loops at positions 686 to 774 and 1408 to 1476 is another specific feature of the PDR subfamily which may have an important role for function. Ref.2
Post-translational modification	Ubiquitinylation mediates endocytosis and vacuolar degradation. Phosphorylation by casein kinase I stabilizes the protein half-life.
Biotechnological use	Strains lacking PDR5 are used for toxicity tests. Strains overexpressing PDR5 are used for screening antifungal sensitizers. Ref.35 Ref.53 Ref.54
Miscellaneous	Present with 42000 molecules/cell in log phase SD medium in log phase SD medium. Ref.50 Full-sized PDR5 orthologs are found only in fungi and plants. Their topology and substrate specificity are distinct from mammalian MDR transporters. This protein has been 'adopted' by Andre Goffeau from the Catholic University of Louvain (Belgium). The above-mentioned scientist has agreed to help us to curate information available on this protein. We are grateful to that person for committing precious time to help producing annotation useful to the whole community. However that person is not responsible for any errors or omissions in this UniProtKB/Swiss-Prot entry. If you have found something wrong or missing in this entry you should submit an update report to: help@uniprot.org.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily. [View classification] Contains 2 ABC transporter domains.
Biophysicochemical properties	Kinetic parameters: Activity measured in plasma membranes. K_M=0.5 mM for MgATP V_max=2.5 µmol/min/mg enzyme pH dependence: Optimum pH is 7.0.
Sequence caution	The sequence BAA05547.1 differs from that shown. Reason: Frameshift at position 61.

Hide | Top

Ontologies

Keywords
Biological process	Antibiotic resistance Cycloheximide resistance Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	drug transport Traceable author statement. Source: SGD response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to cycloheximide Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from expression pattern. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: SGD plasma membrane Ref.18 Ref.43 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct xenobiotic-transporting ATPase activity Traceable author statement. Source: SGD
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-13038,EBI-13038
	P38845	1	EBI-13038,EBI-24770
	P40474	1	EBI-13038,EBI-25203
AGP1	P25376	1	EBI-13038,EBI-2357
CAN1	P04817	1	EBI-13038,EBI-3993
DIP5	P53388	1	EBI-13038,EBI-5904
FMP45	Q07651	1	EBI-13038,EBI-2051056
GAP1	P19145	1	EBI-13038,EBI-7314
HNM1	P19807	1	EBI-13038,EBI-8409
HXT1	P32465	1	EBI-13038,EBI-8759
HXT3	P32466	1	EBI-13038,EBI-8770
HXT5	P38695	1	EBI-13038,EBI-8778
HXT7	P39004	1	EBI-13038,EBI-8790
IST2	P38250	1	EBI-13038,EBI-21520
MUP1	P50276	1	EBI-13038,EBI-11624
PDR12	Q02785	1	EBI-13038,EBI-13065
PMP2	P40975	1	EBI-13038,EBI-2043041
PMP3	P87284	1	EBI-13038,EBI-13555
SDS23	P53172	1	EBI-13038,EBI-23782
SFK1	P35735	1	EBI-13038,EBI-26678
SNQ2	P32568	1	EBI-13038,EBI-17590
SPF1	P39986	1	EBI-13038,EBI-3128
TPO1	Q07824	1	EBI-13038,EBI-38106
TPO2	P53283	1	EBI-13038,EBI-2044753

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1511

1511

Pleiotropic ABC efflux transporter of multiple drugs

PRO_0000093442

Regions

Topological domain

1 – 517

517

Cytoplasmic Potential

Transmembrane

518 – 542

Potential

Topological domain

543 – 558

Extracellular Potential

Transmembrane

559 – 579

Potential

Topological domain

580 – 611

Cytoplasmic Potential

Transmembrane

612 – 628

Potential

Topological domain

629 – 631

Extracellular Potential

Transmembrane

632 – 650

Potential

Topological domain

651 – 665

Cytoplasmic Potential

Transmembrane

666 – 685

Potential

Topological domain

686 – 774

Extracellular Potential

Transmembrane

775 – 793

Potential

Topological domain

794 – 1237

444

Cytoplasmic Potential

Transmembrane

1238 – 1260

Potential

Topological domain

1261 – 1291

Extracellular Potential

Transmembrane

1292 – 1313

Potential

Topological domain

1314 – 1324

Cytoplasmic Potential

Transmembrane

1325 – 1349

Potential

Topological domain

1350 – 1354

Extracellular Potential

Transmembrane

1355 – 1379

Potential

Topological domain

1380 – 1388

Cytoplasmic Potential

Transmembrane

1389 – 1407

Potential

Topological domain

1408 – 1476

Extracellular Potential

Transmembrane

1477 – 1499

Potential

Topological domain

1500 – 1511

Cytoplasmic Potential

Domain

161 – 410

250

ABC transporter 1

Domain

869 – 1112

244

ABC transporter 2

Nucleotide binding

905 – 912

ATP Potential

Compositional bias

784 – 787

Poly-Phe

Amino acid modifications

Modified residue

Phosphothreonine Ref.72

Modified residue

Phosphoserine Ref.72 Ref.76

Modified residue

Phosphoserine Ref.76 Ref.42

Modified residue

Phosphothreonine Ref.76

Modified residue

Phosphoserine Ref.72 Ref.42

Modified residue

837

Phosphoserine Ref.72 Ref.76 Ref.74 Ref.75

Modified residue

840

Phosphoserine Ref.72 Ref.76 Ref.74 Ref.75

Modified residue

841

Phosphoserine Ref.72 Ref.74 Ref.75

Modified residue

849

Phosphoserine Ref.72 Ref.76 Ref.51

Modified residue

850

Phosphoserine Ref.72 Ref.76

Modified residue

854

Phosphoserine Ref.72 Ref.76

Modified residue

857

Phosphotyrosine Ref.51

Modified residue

863

Phosphoserine Ref.51

Glycosylation

734

N-linked (GlcNAc...) Potential

Glycosylation

1447

N-linked (GlcNAc...) Potential

Cross-link

825

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.51 Ref.52

Experimental info

Mutagenesis

183

L → P: Activates ER-associated degradation.

Mutagenesis

257

T → I: Alters drug specificity.

Mutagenesis

302

G → D: Confers generalized drug resistance.

Mutagenesis

648

S → F: Alters drug specificity.

Mutagenesis

905

G → S: Inactivates drug transport.

Mutagenesis

908

G → S: Inactivates drug transport.

Mutagenesis

1009

G → C: Confers generalized drug resistance.

Mutagenesis

1040

G → D: Alters drug specificity.

Mutagenesis

1048

S → V: Alters drug specificity.

Mutagenesis

1289

E → K: Alters drug specificity.

Mutagenesis

1311

Y → S: Alters drug specificity.

Mutagenesis

1360

S → F: Alters drug specificity.

Mutagenesis

1393

T → I: Alters drug specificity.

Mutagenesis

1427

C → Y: Activates ER-associated degradation.

Sequence conflict

171

N → L in BAA05547. Ref.1

Sequence conflict

190

V → I in BAA05547. Ref.1

Sequence conflict

214

D → T in BAA05547. Ref.1

Sequence conflict

308

G → V in BAA05547. Ref.1

Sequence conflict

340 – 345

Missing in BAA05547. Ref.1

Sequence conflict

476

R → H in BAA05547. Ref.1

Sequence conflict

648

Missing in BAA05547. Ref.1

Sequence conflict

770

D → H in BAA05547. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P33302-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4540DC0BF04744BA
Show »

FASTA

1,511

170,438

        10         20         30         40         50         60 
MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL TAQSMQNSTQ 

        70         80         90        100        110        120 
SAPNKSDAQS IFSSGVEGVN PIFSDPEAPG YDPKLDPNSE NFSSAAWVKN MAHLSAADPD 

       130        140        150        160        170        180 
FYKPYSLGCA WKNLSASGAS ADVAYQSTVV NIPYKILKSG LRKFQRSKET NTFQILKPMD 

       190        200        210        220        230        240 
GCLNPGELLV VLGRPGSGCT TLLKSISSNT HGFDLGADTK ISYSGYSGDD IKKHFRGEVV 

       250        260        270        280        290        300 
YNAEADVHLP HLTVFETLVT VARLKTPQNR IKGVDRESYA NHLAEVAMAT YGLSHTRNTK 

       310        320        330        340        350        360 
VGNDIVRGVS GGERKRVSIA EVSICGSKFQ CWDNATRGLD SATALEFIRA LKTQADISNT 

       370        380        390        400        410        420 
SATVAIYQCS QDAYDLFNKV CVLDDGYQIY YGPADKAKKY FEDMGYVCPS RQTTADFLTS 

       430        440        450        460        470        480 
VTSPSERTLN KDMLKKGIHI PQTPKEMNDY WVKSPNYKEL MKEVDQRLLN DDEASREAIK 

       490        500        510        520        530        540 
EAHIAKQSKR ARPSSPYTVS YMMQVKYLLI RNMWRLRNNI GFTLFMILGN CSMALILGSM 

       550        560        570        580        590        600 
FFKIMKKGDT STFYFRGSAM FFAILFNAFS SLLEIFSLYE ARPITEKHRT YSLYHPSADA 

       610        620        630        640        650        660 
FASVLSEIPS KLIIAVCFNI IFYFLVDFRR NGGVFFFYLL INIVAVFSMS HLFRCVGSLT 

       670        680        690        700        710        720 
KTLSEAMVPA SMLLLALSMY TGFAIPKKKI LRWSKWIWYI NPLAYLFESL LINEFHGIKF 

       730        740        750        760        770        780 
PCAEYVPRGP AYANISSTES VCTVVGAVPG QDYVLGDDFI RGTYQYYHKD KWRGFGIGMA 

       790        800        810        820        830        840 
YVVFFFFVYL FLCEYNEGAK QKGEILVFPR SIVKRMKKRG VLTEKNANDP ENVGERSDLS 

       850        860        870        880        890        900 
SDRKMLQESS EEESDTYGEI GLSKSEAIFH WRNLCYEVQI KAETRRILNN VDGWVKPGTL 

       910        920        930        940        950        960 
TALMGASGAG KTTLLDCLAE RVTMGVITGD ILVNGIPRDK SFPRSIGYCQ QQDLHLKTAT 

       970        980        990       1000       1010       1020 
VRESLRFSAY LRQPAEVSIE EKNRYVEEVI KILEMEKYAD AVVGVAGEGL NVEQRKRLTI 

      1030       1040       1050       1060       1070       1080 
GVELTAKPKL LVFLDEPTSG LDSQTAWSIC QLMKKLANHG QAILCTIHQP SAILMQEFDR 

      1090       1100       1110       1120       1130       1140 
LLFMQRGGKT VYFGDLGEGC KTMIDYFESH GAHKCPADAN PAEWMLEVVG AAPGSHANQD 

      1150       1160       1170       1180       1190       1200 
YYEVWRNSEE YRAVQSELDW MERELPKKGS ITAAEDKHEF SQSIIYQTKL VSIRLFQQYW 

      1210       1220       1230       1240       1250       1260 
RSPDYLWSKF ILTIFNQLFI GFTFFKAGTS LQGLQNQMLA VFMFTVIFNP ILQQYLPSFV 

      1270       1280       1290       1300       1310       1320 
QQRDLYEARE RPSRTFSWIS FIFAQIFVEV PWNILAGTIA YFIYYYPIGF YSNASAAGQL 

      1330       1340       1350       1360       1370       1380 
HERGALFWLF SCAFYVYVGS MGLLVISFNQ VAESAANLAS LLFTMSLSFC GVMTTPSAMP 

      1390       1400       1410       1420       1430       1440 
RFWIFMYRVS PLTYFIQALL AVGVANVDVK CADYELLEFT PPSGMTCGQY MEPYLQLAKT 

      1450       1460       1470       1480       1490       1500 
GYLTDENATD TCSFCQISTT NDYLANVNSF YSERWRNYGI FICYIAFNYI AGVFFYWLAR 

      1510 
VPKKNGKLSK K

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae YDR1, which encodes a member of the ATP-binding cassette (ABC) superfamily, is required for multidrug resistance." Hirata D., Yano K., Miyahara K., Miyakawa T. Curr. Genet. 26:285-294(1994) [PubMed: 7882421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[2]	"PDR5, a novel yeast multidrug resistance conferring transporter controlled by the transcription regulator PDR1." Balzi E., Wang M., Leterme S., van Dyck L., Goffeau A. J. Biol. Chem. 269:2206-2214(1994) [PubMed: 8294477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, TOPOLOGY, DOMAINS. Strain: ATCC 204508 / S288c.
[3]	"Molecular cloning and expression of the Saccharomyces cerevisiae STS1 gene product. A yeast ABC transporter conferring mycotoxin resistance." Bissinger P.H., Kuchler K. J. Biol. Chem. 269:4180-4186(1994) [PubMed: 8307980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[4]	"Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV." Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 13:73-83(1997) [PubMed: 9046089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1678.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[6]	"The multidrug resistance gene PDR1 from Saccharomyces cerevisiae." Balzi E., Chen W., Ulaszewski S., Capieaux E., Goffeau A. J. Biol. Chem. 262:16871-16879(1987) [PubMed: 3316228] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[7]	"Interaction of the yeast pleiotropic drug resistance genes PDR1 and PDR5." Meyers S., Schauer W., Balzi E., Wagner M., Goffeau A., Golin J. Curr. Genet. 21:431-436(1992) [PubMed: 1319843] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[8]	"Loss of function mutation in the yeast multiple drug resistance gene PDR5 causes a reduction in chloramphenicol efflux." Leonard P.J., Rathod P.K., Golin J. Antimicrob. Agents Chemother. 38:2492-2494(1994) [PubMed: 7840595] [Abstract] Cited for: SUBSTRATES.
[9]	"Solubilization and characterization of the overexpressed PDR5 multidrug resistance nucleotide triphosphatase of yeast." Decottignies A., Kolaczkowski M., Balzi E., Goffeau A. J. Biol. Chem. 269:12797-12803(1994) [PubMed: 8175692] [Abstract] Cited for: PURIFICATION, BIOPHYSICOCHEMICAL PROPERTIES.
[10]	"Transcriptional control of the yeast PDR5 gene by the PDR3 gene product." Katzmann D.J., Burnett P.E., Golin J., Mahe Y., Moye-Rowley W.S. Mol. Cell. Biol. 14:4653-4661(1994) [PubMed: 8007969] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[11]	"Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance." Delahodde A., Delaveau T., Jacq C. Mol. Cell. Biol. 15:4043-4051(1995) [PubMed: 7623800] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[12]	"Endocytosis and vacuolar degradation of the plasma membrane-localized Pdr5 ATP-binding cassette multidrug transporter in Saccharomyces cerevisiae." Egner R., Mahe Y., Pandjaitan R., Kuchler K. Mol. Cell. Biol. 15:5879-5887(1995) [PubMed: 7565740] [Abstract] Cited for: DEGRADATION.
[13]	"LEM1, an ATP-binding-cassette transporter, selectively modulates the biological potency of steroid hormones." Kralli A., Bohen S.P., Yamamoto K.R. Proc. Natl. Acad. Sci. U.S.A. 92:4701-4705(1995) [PubMed: 7753868] [Abstract] Cited for: FUNCTION.
[14]	"yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae." Miyahara K., Hirata D., Miyakawa T. Curr. Genet. 29:103-105(1996) [PubMed: 8821655] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[15]	"The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole." Egner R., Kuchler K. FEBS Lett. 378:177-181(1996) [PubMed: 8549828] [Abstract] Cited for: DEGRADATION.
[16]	"The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance." Miyahara K., Mizunuma M., Hirata D., Tsuchiya E., Miyakawa T. FEBS Lett. 399:317-320(1996) [PubMed: 8985171] [Abstract] Cited for: FUNCTION.
[17]	"An FK506-sensitive transporter selectively decreases intracellular levels and potency of steroid hormones." Kralli A., Yamamoto K.R. J. Biol. Chem. 271:17152-17156(1996) [PubMed: 8663352] [Abstract] Cited for: INHIBITORS.
[18]	"The ATP binding cassette transporters Pdr5 and Snq2 of Saccharomyces cerevisiae can mediate transport of steroids in vivo." Mahe Y., Lemoine Y., Kuchler K. J. Biol. Chem. 271:25167-25172(1996) [PubMed: 8810273] [Abstract] Cited for: FUNCTION.
[19]	"Anticancer drugs, ionophoric peptides, and steroids as substrates of the yeast multidrug transporter Pdr5p." Kolaczkowski M., van der Rest M., Cybularz-Kolaczkowska A., Soumillion J.P., Konings W.N., Goffeau A. J. Biol. Chem. 271:31543-31548(1996) [PubMed: 8940170] [Abstract] Cited for: FUNCTION.
[20]	"Camptothecin sensitivity is mediated by the pleiotropic drug resistance network in yeast." Reid R.J.D., Kauh E.A., Bjornsti M.-A. J. Biol. Chem. 272:12091-12099(1997) [PubMed: 9115278] [Abstract] Cited for: SUBSTRATES.
[21]	"Clustered amino acid substitutions in the yeast transcription regulator Pdr3p increase pleiotropic drug resistance and identify a new central regulatory domain." Nourani A., Papajova D., Delahodde A., Jacq C., Subik J. Mol. Gen. Genet. 256:397-405(1997) [PubMed: 9393437] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[22]	"Molecular and phenotypic characterization of yeast PDR1 mutants that show hyperactive transcription of various ABC multidrug transporter genes." Carvajal E., van den Hazel H.B., Cybularz-Kolaczkowska A., Balzi E., Goffeau A. Mol. Gen. Genet. 256:406-415(1997) [PubMed: 9393438] [Abstract] Cited for: INDUCTION.
[23]	"Complete inventory of the yeast ABC proteins." Decottignies A., Goffeau A. Nat. Genet. 15:137-145(1997) [PubMed: 9020838] [Abstract] Cited for: PROTEIN FAMILY.
[24]	"Role of ABC transporters in aureobasidin A resistance." Ogawa A., Hashida-Okado T., Endo M., Yoshioka H., Tsuruo T., Takesako K., Kato I. Antimicrob. Agents Chemother. 42:755-761(1998) [PubMed: 9559778] [Abstract] Cited for: SUBSTRATES.
[25]	"Endoplasmic reticulum degradation of a mutated ATP-binding cassette transporter Pdr5 proceeds in a concerted action of Sec61 and the proteasome." Plemper R.K., Egner R., Kuchler K., Wolf D.H. J. Biol. Chem. 273:32848-32856(1998) [PubMed: 9830032] [Abstract] Cited for: TRAFFICKING.
[26]	"Genetic separation of FK506 susceptibility and drug transport in the yeast Pdr5 ATP-binding cassette multidrug resistance transporter." Egner R., Rosenthal F.E., Kralli A., Sanglard D., Kuchler K. Mol. Biol. Cell 9:523-543(1998) [PubMed: 9450972] [Abstract] Cited for: FUNCTION, MUTAGENESIS.
[27]	"In vivo characterization of the drug resistance profile of the major ABC transporters and other components of the yeast pleiotropic drug resistance network." Kolaczkowski M., Kolaczowska A., Luczynski J., Witek S., Goffeau A. Microb. Drug Resist. 4:143-158(1998) [PubMed: 9818966] [Abstract] Cited for: FUNCTION.
[28]	"Casein kinase I-dependent phosphorylation and stability of the yeast multidrug transporter Pdr5p." Decottignies A., Owsianik G., Ghislain M. J. Biol. Chem. 274:37139-37146(1999) [PubMed: 10601275] [Abstract] Cited for: PHOSPHORYLATION BY CK1.
[29]	"Chemical specificity of the PDR5 multidrug resistance gene product of Saccharomyces cerevisiae based on studies with tri-n-alkyltin chlorides." Golin J., Barkatt A., Cronin S., Eng G., May L. Antimicrob. Agents Chemother. 44:134-138(2000) [PubMed: 10602734] [Abstract] Cited for: SUBSTRATES.
[30]	"Role of the PDR gene network in yeast susceptibility to the antifungal antibiotic mucidin." Michalkova-Papajova D., Obernauerova M., Subik J. Antimicrob. Agents Chemother. 44:418-420(2000) [PubMed: 10639374] [Abstract] Cited for: SUBSTRATES.
[31]	"The transmembrane domain 10 of the yeast Pdr5p ABC antifungal efflux pump determines both substrate specificity and inhibitor susceptibility." Egner R., Bauer B.E., Kuchler K. Mol. Microbiol. 35:1255-1263(2000) [PubMed: 10712705] [Abstract] Cited for: TOPOLOGY, MUTAGENESIS.
[32]	"Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants." DeRisi J., van den Hazel B., Marc P., Balzi E., Brown P., Jacq C., Goffeau A. FEBS Lett. 470:156-160(2000) [PubMed: 10734226] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[33]	"Prenyl-flavonoids as potent inhibitors of the Pdr5p multidrug ABC transporter from Saccharomyces cerevisiae." Conseil G., Decottignies A., Jault J.-M., Comte G., Barron D., Goffeau A., Di Pietro A. Biochemistry 39:6910-6917(2000) [PubMed: 10841772] [Abstract] Cited for: FUNCTION, INHIBITORS.
[34]	"Protein kinase C effectors bind to multidrug ABC transporters and inhibit their activity." Conseil G., Perez-Victoria J.M., Jault J.-M., Gamarro F., Goffeau A., Hofmann J., Di Pietro A. Biochemistry 40:2564-2571(2001) [PubMed: 11327879] [Abstract] Cited for: INHIBITORS.
[35]	"A novel screening for inhibitors of a pleiotropic drug resistant pump, Pdr5, in Saccharomyces cerevisiae." Hiraga K., Wanigasekera A., Sugi H., Hamanaka N., Oda K. Biosci. Biotechnol. Biochem. 65:1589-1595(2001) [PubMed: 11515543] [Abstract] Cited for: BIOTECHNOLOGY.
[36]	"Purification and some properties of an inhibitor for a yeast pleiotropic drug resistant pump from Kitasatospora sp. E-420." Wanigasekera A., Hiraga K., Hamanaka N., Oda K. Biosci. Biotechnol. Biochem. 65:2353-2357(2001) [PubMed: 11758940] [Abstract] Cited for: INHIBITORS.
[37]	"The ABC transporter Pdr5p mediates the efflux of nonsteroidal ecdysone agonists in Saccharomyces cerevisiae." Hu W., Feng Q., Palli S.R., Krell P.J., Arif B.M., Retnakaran A. Eur. J. Biochem. 268:3416-3422(2001) [PubMed: 11422371] [Abstract] Cited for: SUBSTRATES.
[38]	"Saccharomyces cerevisiae multidrug resistance gene expression inversely correlates with the status of the F(0) component of the mitochondrial ATPase." Zhang X., Moye-Rowley W.S. J. Biol. Chem. 276:47844-47852(2001) [PubMed: 11602584] [Abstract] Cited for: METABOLIC REGULATION.
[39]	"The pleitropic drug ABC transporters from Saccharomyces cerevisiae." Rogers B., Decottignies A., Kolaczkowski M., Carvajal E., Balzi E., Goffeau A. J. Mol. Microbiol. Biotechnol. 3:207-214(2001) [PubMed: 11321575] [Abstract] Cited for: FUNCTION.
[40]	"Zinc cluster protein Rdr1p is a transcriptional repressor of the PDR5 gene encoding a multidrug transporter." Hellauer K., Akache B., MacPherson S., Sirard E., Turcotte B. J. Biol. Chem. 277:17671-17676(2002) [PubMed: 11882665] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[41]	"New regulators of drug sensitivity in the family of yeast zinc cluster proteins." Akache B., Turcotte B. J. Biol. Chem. 277:21254-21260(2002) [PubMed: 11943786] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[42]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58 AND SER-61, MASS SPECTROMETRY.
[43]	"Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae." Navarre C., Degand H., Bennett K.L., Crawford J.S., Moertz E., Boutry M. Proteomics 2:1706-1714(2002) [PubMed: 12469340] [Abstract] Cited for: SUBCELLULAR LOCATION.
[44]	"Potent competitive inhibition of drug binding to the Saccharomyces cerevisiae ABC exporter Pdr5p by the hydrophobic estradiol-derivative RU49953." Conseil G., Perez-Victoria J.M., Renoir J.M., Goffeau A., Di Pietro A. Biochim. Biophys. Acta 1614:131-134(2003) [PubMed: 12896805] [Abstract] Cited for: FUNCTION, INHIBITORS.
[45]	"Phenothiazines as potent modulators of yeast multidrug resistance." Kolaczkowski M., Michalak K., Motohashi N. Int. J. Antimicrob. Agents 22:279-283(2003) [PubMed: 13678835] [Abstract] Cited for: FUNCTION, INHIBITORS.
[46]	"Studies with novel Pdr5p substrates demonstrate a strong size dependence for xenobiotic efflux." Golin J., Ambudkar S.V., Gottesman M.M., Habib A.D., Sczepanski J., Ziccardi W., May L. J. Biol. Chem. 278:5963-5969(2003) [PubMed: 12496287] [Abstract] Cited for: SUBSTRATES.
[47]	"A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast." Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C. J. Biol. Chem. 278:11427-11432(2003) [PubMed: 12529331] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[48]	"Three-dimensional reconstruction of the Saccharomyces cerevisiae multidrug resistance protein Pdr5p." Ferreira-Pereira A., Marco S., Decottignies A., Nader J., Goffeau A., Rigaud J.L. J. Biol. Chem. 278:11995-11999(2003) [PubMed: 12551908] [Abstract] Cited for: PURIFICATION, ELECTRON MICROSCOPY.
[49]	"Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis." Gupta S.S., Ton V.-K., Beaudry V., Rulli S., Cunningham K., Rao R. J. Biol. Chem. 278:28831-28839(2003) [PubMed: 12754197] [Abstract] Cited for: SUBSTRATES.
[50]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[51]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-849; TYR-857 AND SER-863, UBIQUITINATION AT LYS-825, MASS SPECTROMETRY.
[52]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-825, MASS SPECTROMETRY.
[53]	"A yeast-based method for the detection of cyto and genotoxicity." Lichtenberg-Frate H., Schmitt M., Gellert G., Ludwig J. Toxicol. in Vitro 17:709-716(2003) [PubMed: 14599467] [Abstract] Cited for: BIOTECHNOLOGY.
[54]	"Chemosensitization of fluconazole resistance in Saccharomyces cerevisiae and pathogenic fungi by a D-octapeptide derivative." Niimi K., Harding D.R., Parshot R., King A., Lun D.J., Decottignies A., Niimi M., Lin S., Cannon R.D., Goffeau A., Monk B.C. Antimicrob. Agents Chemother. 48:1256-1271(2004) [PubMed: 15047528] [Abstract] Cited for: BIOTECHNOLOGY.
[55]	"The transporters Pdr5p and Snq2p mediate diazaborine resistance and are under the control of the gain-of-function allele PDR1-12." Wehrschutz-Sigl E., Jungwirth H., Bergler H., Hogenauer G. Eur. J. Biochem. 271:1145-1152(2004) [PubMed: 15009193] [Abstract] Cited for: SUBSTRATES.
[56]	"Expression regulation of the yeast PDR5 ATP-binding cassette (ABC) transporter suggests a role in cellular detoxification during the exponential growth phase." Mamnun Y.M., Schuller C., Kuchler K. FEBS Lett. 559:111-117(2004) [PubMed: 14960317] [Abstract] Cited for: FUNCTION.
[57]	"On the mechanism of constitutive Pdr1 activator-mediated PDR5 transcription in Saccharomyces cerevisiae: evidence for enhanced recruitment of coactivators and altered nucleosome structures." Gao C., Wang L., Milgrom E., Shen W.C. J. Biol. Chem. 279:42677-42686(2004) [PubMed: 15294907] [Abstract] Cited for: INDUCTION.
[58]	"Enniatin has a new function as an inhibitor of Pdr5p, one of the ABC transporters in Saccharomyces cerevisiae." Hiraga K., Yamamoto S., Fukuda H., Hamanaka N., Oda K. Biochem. Biophys. Res. Commun. 328:1119-1125(2005) [PubMed: 15707993] [Abstract] Cited for: INHIBITORS.
[59]	"A new function of isonitrile as an inhibitor of the Pdr5p multidrug ABC transporter in Saccharomyces cerevisiae." Yamamoto S., Hiraga K., Abiko A., Hamanaka N., Oda K. Biochem. Biophys. Res. Commun. 330:622-628(2005) [PubMed: 15796929] [Abstract] Cited for: INHIBITORS.
[60]	"The role of hydrogen bond acceptor groups in the interaction of substrates with Pdr5p, a major yeast drug transporter." Hanson L., May L., Tuma P., Keeven J., Mehl P., Ferenz M., Ambudkar S.V., Golin J. Biochemistry 44:9703-9713(2005) [PubMed: 16008355] [Abstract] Cited for: SUBSTRATES.
[61]	"Retrograde regulation of multidrug resistance in Saccharomyces cerevisiae." Moye-Rowley W.S. Gene 354:15-21(2005) [PubMed: 15896930] [Abstract] Cited for: METABOLIC REGULATION.
[62]	"Mutational analysis of the yeast multidrug resistance ABC transporter Pdr5p with altered drug specificity." Tutulan-Cunita A.C., Mikoshi M., Mizunuma M., Hirata D., Miyakawa T. Genes Cells 10:409-420(2005) [PubMed: 15836770] [Abstract] Cited for: FUNCTION, MUTAGENESIS.
[63]	"Activity of yeast multidrug resistance pumps during growth is controlled by carbon source and the composition of growth-depleted medium: DiS-C3(3) fluorescence assay." Malac J., Urbankova E., Sigler K., Gaskova D. Int. J. Biochem. Cell Biol. 37:2536-2543(2005) [PubMed: 16061415] [Abstract] Cited for: INDUCTION.
[64]	"Regulation of the sphingoid long-chain base kinase Lcb4p by ergosterol and heme: studies in phytosphingosine-resistant mutants." Sano T., Kihara A., Kurotsu F., Iwaki S., Igarashi Y. J. Biol. Chem. 280:36674-36682(2005) [PubMed: 16141212] [Abstract] Cited for: SUBSTRATES.
[65]	"Early expression of yeast genes affected by chemical stress." Lucau-Danila A., Lelandais G., Kozovska Z., Tanty V., Delaveau T., Devaux F., Jacq C. Mol. Cell. Biol. 25:1860-1868(2005) [PubMed: 15713640] [Abstract] Cited for: INDUCTION.
[66]	"Early transcriptional response of Saccharomyces cerevisiae to stress imposed by the herbicide 2,4-dichlorophenoxyacetic acid." Teixeira M.C., Fernandes A.R., Mira N.P., Becker J.D., Sa-Correia I. FEMS Yeast Res. 6:230-248(2006) [PubMed: 16487346] [Abstract] Cited for: INDUCTION.
[67]	"Adaptive response to the antimalarial drug artesunate in yeast involves Pdr1p/Pdr3p-mediated transcriptional activation of the resistance determinants TPO1 and PDR5." Alenquer M., Tenreiro S., Sa-Correia I. FEMS Yeast Res. 6:1130-1139(2006) [PubMed: 17156010] [Abstract] Cited for: INDUCTION.
[68]	"ELM1 is required for multidrug resistance in Saccharomyces cerevisiae." Souid A.K., Gao C., Wang L., Milgrom E., Shen W.C. Genetics 173:1919-1937(2006) [PubMed: 16751665] [Abstract] Cited for: METABOLIC REGULATION, FUNCTION.
[69]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[70]	"The yeast Pdr5p multidrug transporter: how does it recognize so many substrates?" Golin J., Ambudkar S.V., May L. Biochem. Biophys. Res. Commun. 356:1-5(2007) [PubMed: 17316560] [Abstract] Cited for: FUNCTION.
[71]	"The central role of PDR1 in the foundation of yeast drug resistance." Fardeau V., Lelandais G., Oldfield A., Salin H., Lemoine S., Garcia M., Tanty V., Le Crom S., Jacq C., Devaux F. J. Biol. Chem. 282:5063-5074(2007) [PubMed: 17158869] [Abstract] Cited for: TRANSCRIPTION REGULATION.
[72]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-49; SER-54; SER-61; SER-837; SER-840; SER-841; SER-849; SER-850 AND SER-854, MASS SPECTROMETRY.
[73]	"Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding fold." de Thozee C.P., Cronin S., Goj A., Golin J., Ghislain M. Mol. Microbiol. 63:811-825(2007) [PubMed: 17302805] [Abstract] Cited for: TRAFFICKING.
[74]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-837; SER-840 AND SER-841, MASS SPECTROMETRY.
[75]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-837; SER-840 AND SER-841, MASS SPECTROMETRY.
[76]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-54; SER-58; THR-59; SER-837; SER-840; SER-849; SER-850 AND SER-854, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	D26548 Genomic DNA. Translation: BAA05547.1. Frameshift. X74113 Genomic DNA. Translation: CAA52212.1. L19922 Genomic DNA. Translation: AAB53769.1. U55020 Genomic DNA. Translation: AAC49639.1. Z75061 Genomic DNA. Translation: CAA99359.1.
PIR	A53151.
RefSeq	NP_014796.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6776N.
IntAct	P33302. 39 interactions.
Protein family/group databases
TCDB	3.A.1.205.1. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PeptideAtlas	P33302.
Genome annotation databases
Ensembl	YOR153W. Saccharomyces cerevisiae. [Contig view]
GeneID	854324.
GenomeReviews	Gene locus YOR153W in contig Y13140_GR.
KEGG	sce:YOR153W.
NMPDR	fig\|4932.3.peg.5901.
Organism-specific databases
CYGD	YOR153w.
SGD	S000005679. PDR5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P33302.
OMA	P33302. AYANISS.
Gene expression databases
ArrayExpress	P33302.
GermOnline	YOR153W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013525. ABC_2_trans. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. IPR010929. PDR_CDR_ABC. IPR005285. Pleiotrop_drug-R_PDR. [Graphical view]
Pfam	PF01061. ABC2_membrane. 2 hits. PF00005. ABC_tran. 2 hits. PF06422. PDR_CDR. 1 hit. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
TIGRFAMs	TIGR00956. 3a01205. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976366.

Hide | Top

Entry information

Entry name

PDR5_YEAST

Accession

Primary (citable) accession number: P33302

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	July 7, 2009
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents