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Protein

26S proteasome regulatory subunit 7 homolog

Gene

RPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Miscellaneous

Present with 105 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 257ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent ERAD pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31920-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 7 homolog
Alternative name(s):
Protein CIM5
Tat-binding homolog 3
Gene namesi
Name:RPT1
Synonyms:CIM5, YTA3
Ordered Locus Names:YKL145W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL145W
SGDiS000001628 RPT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847191 – 46726S proteasome regulatory subunit 7 homologAdd BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33299
PaxDbiP33299
PRIDEiP33299

2D gel databases

SWISS-2DPAGEiP33299

PTM databases

iPTMnetiP33299

Interactioni

Subunit structurei

Interacts with UBR1 and CIC1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33991, 572 interactors
DIPiDIP-2883N
IntActiP33299, 87 interactors
MINTiP33299
STRINGi4932.YKL145W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80H1-467[»]
3JCPelectron microscopy4.60H1-467[»]
3VLFX-ray3.80B/D381-467[»]
4A3VX-ray3.80B/D378-467[»]
4CR2electron microscopy7.70H1-467[»]
4CR3electron microscopy9.30H1-467[»]
4CR4electron microscopy8.80H1-467[»]
4JPOX-ray5.00C/D379-467[»]
5A5Belectron microscopy9.50H1-467[»]
5MP9electron microscopy4.10H1-467[»]
5MPAelectron microscopy4.50H1-467[»]
5MPBelectron microscopy7.80H1-467[»]
5MPCelectron microscopy7.70H1-467[»]
5WVIelectron microscopy6.30H1-467[»]
5WVKelectron microscopy4.20H1-467[»]
ProteinModelPortaliP33299
SMRiP33299
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074978
HOGENOMiHOG000225143
InParanoidiP33299
KOiK03061
OMAiNKYQIHL
OrthoDBiEOG092C19WS

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035245 PSMC2
PANTHERiPTHR23073:SF13 PTHR23073:SF13, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P33299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK
60 70 80 90 100
QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR LGEEHPLQVA
110 120 130 140 150
RCTKIIKGNG ESDETTTDNN NSGNSNSNSN QQSTDADEDD EDAKYVINLK
160 170 180 190 200
QIAKFVVGLG ERVSPTDIEE GMRVGVDRSK YNIELPLPPR IDPSVTMMTV
210 220 230 240 250
EEKPDVTYSD VGGCKDQIEK LREVVELPLL SPERFATLGI DPPKGILLYG
260 270 280 290 300
PPGTGKTLCA RAVANRTDAT FIRVIGSELV QKYVGEGARM VRELFEMART
310 320 330 340 350
KKACIIFFDE IDAVGGARFD DGAGGDNEVQ RTMLELITQL DGFDPRGNIK
360 370 380 390 400
VMFATNRPNT LDPALLRPGR IDRKVEFSLP DLEGRANIFR IHSKSMSVER
410 420 430 440 450
GIRWELISRL CPNSTGAELR SVCTEAGMFA IRARRKVATE KDFLKAVDKV
460
ISGYKKFSST SRYMQYN
Length:467
Mass (Da):51,983
Last modified:February 1, 1994 - v1
Checksum:i8454BBC9D549EC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22817 Genomic DNA Translation: CAA80470.1
X73571 Genomic DNA Translation: CAA51973.1
Z28145 Genomic DNA Translation: CAA81986.1
BK006944 Genomic DNA Translation: DAA09017.1
PIRiS34354
RefSeqiNP_012777.1, NM_001179711.1

Genome annotation databases

EnsemblFungiiYKL145W; YKL145W; YKL145W
GeneIDi853712
KEGGisce:YKL145W

Similar proteinsi

Entry informationi

Entry nameiPRS7_YEAST
AccessioniPrimary (citable) accession number: P33299
Secondary accession number(s): D6VX51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health