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Protein

26S protease regulatory subunit 7 homolog

Gene

RPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Miscellaneous

Present with 105 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 257ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent ERAD pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31920-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 7 homolog
Alternative name(s):
Protein CIM5
Tat-binding homolog 3
Gene namesi
Name:RPT1
Synonyms:CIM5, YTA3
Ordered Locus Names:YKL145W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL145W.
SGDiS000001628. RPT1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847191 – 46726S protease regulatory subunit 7 homologAdd BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33299.
PRIDEiP33299.

2D gel databases

SWISS-2DPAGEiP33299.

PTM databases

iPTMnetiP33299.

Interactioni

Subunit structurei

Interacts with UBR1 and CIC1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33991. 568 interactors.
DIPiDIP-2883N.
IntActiP33299. 83 interactors.
MINTiMINT-612502.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80H1-467[»]
3JCPelectron microscopy4.60H1-467[»]
3VLFX-ray3.80B/D381-467[»]
4A3VX-ray3.80B/D378-467[»]
4CR2electron microscopy7.70H1-467[»]
4CR3electron microscopy9.30H1-467[»]
4CR4electron microscopy8.80H1-467[»]
4JPOX-ray5.00C/D379-467[»]
5A5Belectron microscopy9.50H1-467[»]
5MP9electron microscopy4.10H1-467[»]
5MPAelectron microscopy4.50H1-467[»]
5MPBelectron microscopy7.80H1-467[»]
5MPCelectron microscopy7.70H1-467[»]
5WVIelectron microscopy6.30H1-467[»]
5WVKelectron microscopy4.20H1-467[»]
ProteinModelPortaliP33299.
SMRiP33299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074978.
HOGENOMiHOG000225143.
InParanoidiP33299.
KOiK03061.
OMAiFQVARCT.
OrthoDBiEOG092C19WS.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

P33299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK
60 70 80 90 100
QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR LGEEHPLQVA
110 120 130 140 150
RCTKIIKGNG ESDETTTDNN NSGNSNSNSN QQSTDADEDD EDAKYVINLK
160 170 180 190 200
QIAKFVVGLG ERVSPTDIEE GMRVGVDRSK YNIELPLPPR IDPSVTMMTV
210 220 230 240 250
EEKPDVTYSD VGGCKDQIEK LREVVELPLL SPERFATLGI DPPKGILLYG
260 270 280 290 300
PPGTGKTLCA RAVANRTDAT FIRVIGSELV QKYVGEGARM VRELFEMART
310 320 330 340 350
KKACIIFFDE IDAVGGARFD DGAGGDNEVQ RTMLELITQL DGFDPRGNIK
360 370 380 390 400
VMFATNRPNT LDPALLRPGR IDRKVEFSLP DLEGRANIFR IHSKSMSVER
410 420 430 440 450
GIRWELISRL CPNSTGAELR SVCTEAGMFA IRARRKVATE KDFLKAVDKV
460
ISGYKKFSST SRYMQYN
Length:467
Mass (Da):51,983
Last modified:February 1, 1994 - v1
Checksum:i8454BBC9D549EC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22817 Genomic DNA. Translation: CAA80470.1.
X73571 Genomic DNA. Translation: CAA51973.1.
Z28145 Genomic DNA. Translation: CAA81986.1.
BK006944 Genomic DNA. Translation: DAA09017.1.
PIRiS34354.
RefSeqiNP_012777.1. NM_001179711.1.

Genome annotation databases

EnsemblFungiiYKL145W; YKL145W; YKL145W.
GeneIDi853712.
KEGGisce:YKL145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22817 Genomic DNA. Translation: CAA80470.1.
X73571 Genomic DNA. Translation: CAA51973.1.
Z28145 Genomic DNA. Translation: CAA81986.1.
BK006944 Genomic DNA. Translation: DAA09017.1.
PIRiS34354.
RefSeqiNP_012777.1. NM_001179711.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80H1-467[»]
3JCPelectron microscopy4.60H1-467[»]
3VLFX-ray3.80B/D381-467[»]
4A3VX-ray3.80B/D378-467[»]
4CR2electron microscopy7.70H1-467[»]
4CR3electron microscopy9.30H1-467[»]
4CR4electron microscopy8.80H1-467[»]
4JPOX-ray5.00C/D379-467[»]
5A5Belectron microscopy9.50H1-467[»]
5MP9electron microscopy4.10H1-467[»]
5MPAelectron microscopy4.50H1-467[»]
5MPBelectron microscopy7.80H1-467[»]
5MPCelectron microscopy7.70H1-467[»]
5WVIelectron microscopy6.30H1-467[»]
5WVKelectron microscopy4.20H1-467[»]
ProteinModelPortaliP33299.
SMRiP33299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33991. 568 interactors.
DIPiDIP-2883N.
IntActiP33299. 83 interactors.
MINTiMINT-612502.

PTM databases

iPTMnetiP33299.

2D gel databases

SWISS-2DPAGEiP33299.

Proteomic databases

MaxQBiP33299.
PRIDEiP33299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL145W; YKL145W; YKL145W.
GeneIDi853712.
KEGGisce:YKL145W.

Organism-specific databases

EuPathDBiFungiDB:YKL145W.
SGDiS000001628. RPT1.

Phylogenomic databases

GeneTreeiENSGT00550000074978.
HOGENOMiHOG000225143.
InParanoidiP33299.
KOiK03061.
OMAiFQVARCT.
OrthoDBiEOG092C19WS.

Enzyme and pathway databases

BioCyciYEAST:G3O-31920-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiPR:P33299.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRS7_YEAST
AccessioniPrimary (citable) accession number: P33299
Secondary accession number(s): D6VX51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 10, 2017
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.