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Protein

26S proteasome regulatory subunit 6B homolog

Gene

RPT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi213 – 220ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent ERAD pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29942-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 6B homolog
Alternative name(s):
Protein YNT1
Tat-binding homolog 2
Gene namesi
Name:RPT3
Synonyms:YNT1, YTA2
Ordered Locus Names:YDR394W
ORF Names:D9509.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR394W
SGDiS000002802 RPT3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846971 – 42826S proteasome regulatory subunit 6B homologAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Cross-linki280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

N-acetylated by NAT3.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP33298
PaxDbiP33298
PRIDEiP33298

PTM databases

iPTMnetiP33298

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • TBP-class protein binding Source: GO_Central

Protein-protein interaction databases

BioGridi32456, 418 interactors
DIPiDIP-1587N
IntActiP33298, 104 interactors
MINTiP33298
STRINGi4932.YDR394W

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi351 – 359Combined sources9
Helixi368 – 373Combined sources6
Helixi380 – 396Combined sources17
Beta strandi400 – 402Combined sources3
Helixi404 – 412Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DZNX-ray2.20B/D/F348-428[»]
2DZOX-ray3.00B/D348-428[»]
3JCOelectron microscopy4.80K1-428[»]
3JCPelectron microscopy4.60K1-428[»]
4CR2electron microscopy7.70K1-428[»]
4CR3electron microscopy9.30K1-428[»]
4CR4electron microscopy8.80K1-428[»]
5A5Belectron microscopy9.50K1-428[»]
5MP9electron microscopy4.10K1-428[»]
5MPAelectron microscopy4.50K1-428[»]
5MPBelectron microscopy7.80K1-428[»]
5MPCelectron microscopy7.70K1-428[»]
5WVIelectron microscopy6.30K1-428[»]
5WVKelectron microscopy4.20K1-428[»]
ProteinModelPortaliP33298
SMRiP33298
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33298

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074962
HOGENOMiHOG000225143
InParanoidiP33298
KOiK03063
OMAiLIIRNDP
OrthoDBiEOG092C19WS

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035256 PSMC4
PANTHERiPTHR23073:SF8 PTHR23073:SF8, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P33298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELGIVTPV EKAVEEKPAV KSYASLLAQL NGTVNNNSAL SNVNSDIYFK
60 70 80 90 100
LKKLEKEYEL LTLQEDYIKD EQRHLKRELK RAQEEVKRIQ SVPLVIGQFL
110 120 130 140 150
EPIDQNTGIV SSTTGMSYVV RILSTLDREL LKPSMSVALH RHSNALVDIL
160 170 180 190 200
PPDSDSSISV MGENEKPDVT YADVGGLDMQ KQEIREAVEL PLVQADLYEQ
210 220 230 240 250
IGIDPPRGVL LYGPPGTGKT MLVKAVANST KAAFIRVNGS EFVHKYLGEG
260 270 280 290 300
PRMVRDVFRL ARENAPSIIF IDEVDSIATK RFDAQTGSDR EVQRILIELL
310 320 330 340 350
TQMDGFDQST NVKVIMATNR ADTLDPALLR PGRLDRKIEF PSLRDRRERR
360 370 380 390 400
LIFGTIASKM SLAPEADLDS LIIRNDSLSG AVIAAIMQEA GLRAVRKNRY
410 420
VILQSDLEEA YATQVKTDNT VDKFDFYK
Length:428
Mass (Da):47,894
Last modified:October 5, 2010 - v2
Checksum:iE12F0995A60732BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti342S → Y in CAA51972 (PubMed:7754704).Curated1
Sequence conflicti342S → Y in AAA81916 (PubMed:7803857).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73570 Genomic DNA Translation: CAA51972.1
U06229 Genomic DNA Translation: AAA81916.1
U32274 Genomic DNA Translation: AAB64836.1
BK006938 Genomic DNA Translation: DAA12238.1
PIRiS69678
RefSeqiNP_010682.3, NM_001180702.3

Genome annotation databases

EnsemblFungiiYDR394W; YDR394W; YDR394W
GeneIDi852003
KEGGisce:YDR394W

Similar proteinsi

Entry informationi

Entry nameiPRS6B_YEAST
AccessioniPrimary (citable) accession number: P33298
Secondary accession number(s): D6VT28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 5, 2010
Last modified: March 28, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health