Reviewed, UniProtKB/Swiss-Prot P33298 (PRS6B_YEAST)

Names and origin

Protein names

Recommended name:
    26S protease regulatory subunit 6B homolog
Alternative name(s):
    Protein YNT1
    TAT-binding homolog 2

Gene names

Name:	RPT3
Synonyms:	YNT1, YTA2
Ordered Locus Names:	YDR394W
ORF Names:	D9509.14

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex By similarity.
Subcellular location	Cytoplasm Potential. Nucleus Potential.
Post-translational modification	N-acetylated by NAT3.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Traceable author statement. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome regulatory particle, base subcomplex Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from direct assay. Source: SGD endopeptidase activity Traceable author statement. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-13905,EBI-13905
NAS6	P50086	3	EBI-13905,EBI-14028
NAT3	Q06504	1	EBI-13905,EBI-31002
RPT1	P33299	1	EBI-13905,EBI-13910
RPT5	P33297	4	EBI-13905,EBI-13920
RPT6	Q01939	1	EBI-13905,EBI-13914

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 428

428

26S protease regulatory subunit 6B homolog

PRO_0000084697

Regions

Nucleotide binding

213 – 220

8

ATP Potential

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.4 Ref.5

Modified residue

8

1

Phosphothreonine Ref.5 Ref.6

Experimental info

Sequence conflict

342

1

Y → S in AAB64836. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

428

Helix Strand Turn

Details...

Helix

380 – 396

17

Beta strand

400 – 402

3

Helix

404 – 412

9

Sequences

	Sequence		Length	Mass (Da)
	P33298-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EB8FA39F0C073FDD	FASTA	428	47,970
	10 20 30 40 50 60 MEELGIVTPV EKAVEEKPAV KSYASLLAQL NGTVNNNSAL SNVNSDIYFK LKKLEKEYEL 70 80 90 100 110 120 LTLQEDYIKD EQRHLKRELK RAQEEVKRIQ SVPLVIGQFL EPIDQNTGIV SSTTGMSYVV 130 140 150 160 170 180 RILSTLDREL LKPSMSVALH RHSNALVDIL PPDSDSSISV MGENEKPDVT YADVGGLDMQ 190 200 210 220 230 240 KQEIREAVEL PLVQADLYEQ IGIDPPRGVL LYGPPGTGKT MLVKAVANST KAAFIRVNGS 250 260 270 280 290 300 EFVHKYLGEG PRMVRDVFRL ARENAPSIIF IDEVDSIATK RFDAQTGSDR EVQRILIELL 310 320 330 340 350 360 TQMDGFDQST NVKVIMATNR ADTLDPALLR PGRLDRKIEF PYLRDRRERR LIFGTIASKM 370 380 390 400 410 420 SLAPEADLDS LIIRNDSLSG AVIAAIMQEA GLRAVRKNRY VILQSDLEEA YATQVKTDNT VDKFDFYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex." Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H. Yeast 10:1141-1155(1994) [PubMed: 7754704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C836.
[2]	Campbell C.L., Tanaka N., White K.H., Thorsness P.E. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome." Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H. Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6, ACETYLATION AT MET-1.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION AT THR-8, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X73570 Genomic DNA. Translation: CAA51972.1.
U06229 Genomic DNA. Translation: AAA81916.1.
U32274 Genomic DNA. Translation: AAB64836.1.

PIR

S69678.

RefSeq

NP_010682.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DZN	X-ray	2.20	B/D/F	348-428	[»]
2DZO	X-ray	3.00	B/D	348-428	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1587N.

IntAct

P33298. 72 interactions.

STRING

P33298.

Proteomic databases

PeptideAtlas

P33298.

PRIDE

P33298.

Genome annotation databases

Ensembl

YDR394W; YDR394W; YDR394W; Saccharomyces cerevisiae. [Genome view]

GeneID

852003.

KEGG

sce:YDR394W.

Organism-specific databases

CYGD

YDR394w.

SGD

S000002802. RPT3.

Phylogenomic databases

HOGENOM

HBG724153.

OrthoDB

EOG9QRJMR.

Gene expression databases

ArrayExpress

P33298.

Genevestigator

P33298.

GermOnline

YDR394W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005937. 26S_Psome_P45.
IPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
[Graphical view]

Pfam

PF00004. AAA. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

PROSITE

PS00674. AAA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PRS6B_YEAST

Accession

Primary (citable) accession number: P33298

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 15, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)