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Protein

26S proteasome regulatory subunit 6A

Gene

RPT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 229ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent ERAD pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33646-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 6A
Alternative name(s):
Tat-binding protein homolog 1
Short name:
TBP-1
Gene namesi
Name:RPT5
Synonyms:YTA1
Ordered Locus Names:YOR117W
ORF Names:O3258, YOR3258W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR117W
SGDiS000005643 RPT5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000847082 – 43426S proteasome regulatory subunit 6AAdd BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei180PhosphotyrosineCombined sources1

Post-translational modificationi

N-acetylated by NAT1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP33297
PaxDbiP33297
PRIDEiP33297

PTM databases

iPTMnetiP33297

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34513624 interactors.
DIPiDIP-1590N
IntActiP33297 62 interactors.
MINTiP33297
STRINGi4932.YOR117W

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi356 – 365Combined sources10
Helixi376 – 381Combined sources6
Turni382 – 385Combined sources4
Helixi388 – 404Combined sources17
Beta strandi408 – 410Combined sources3
Helixi412 – 427Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80M1-434[»]
3JCPelectron microscopy4.60M1-434[»]
3WHKX-ray2.60A/B/C/D/E/F/G/H356-434[»]
3WHLX-ray4.00A/C/E/G356-434[»]
4CR2electron microscopy7.70M1-434[»]
4CR3electron microscopy9.30M1-434[»]
4CR4electron microscopy8.80M1-434[»]
5A5Belectron microscopy9.50M1-434[»]
5MP9electron microscopy4.10M1-434[»]
5MPAelectron microscopy4.50M1-434[»]
5MPBelectron microscopy7.80M1-434[»]
5MPCelectron microscopy7.70M1-434[»]
5WVIelectron microscopy6.30M1-434[»]
5WVKelectron microscopy4.20M1-434[»]
ProteinModelPortaliP33297
SMRiP33297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000111070
HOGENOMiHOG000225143
InParanoidiP33297
KOiK03065
OMAiPMKEADR
OrthoDBiEOG092C19WS

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035254 PSMC3
PANTHERiPTHR23073:SF7 PTHR23073:SF7, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLEELDAQ TLPGDDELDQ EILNLSTQEL QTRAKLLDNE IRIFRSELQR
60 70 80 90 100
LSHENNVMLE KIKDNKEKIK NNRQLPYLVA NVVEVMDMNE IEDKENSEST
110 120 130 140 150
TQGGNVNLDN TAVGKAAVVK TSSRQTVFLP MVGLVDPDKL KPNDLVGVNK
160 170 180 190 200
DSYLILDTLP SEFDSRVKAM EVDEKPTETY SDVGGLDKQI EELVEAIVLP
210 220 230 240 250
MKRADKFKDM GIRAPKGALM YGPPGTGKTL LARACAAQTN ATFLKLAAPQ
260 270 280 290 300
LVQMYIGEGA KLVRDAFALA KEKAPTIIFI DELDAIGTKR FDSEKSGDRE
310 320 330 340 350
VQRTMLELLN QLDGFSSDDR VKVLAATNRV DVLDPALLRS GRLDRKIEFP
360 370 380 390 400
LPSEDSRAQI LQIHSRKMTT DDDINWQELA RSTDEFNGAQ LKAVTVEAGM
410 420 430
IALRNGQSSV KHEDFVEGIS EVQARKSKSV SFYA
Length:434
Mass (Da):48,256
Last modified:January 23, 2007 - v3
Checksum:iC215422033FCE830
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73569 Genomic DNA Translation: CAA51971.1
X90518 Genomic DNA Translation: CAA62114.1
X94335 Genomic DNA Translation: CAA64037.1
Z75025 Genomic DNA Translation: CAA99315.1
BK006948 Genomic DNA Translation: DAA10892.1
PIRiS46605
RefSeqiNP_014760.3, NM_001183536.3

Genome annotation databases

EnsemblFungiiYOR117W; YOR117W; YOR117W
GeneIDi854284
KEGGisce:YOR117W

Similar proteinsi

Entry informationi

Entry nameiPRS6A_YEAST
AccessioniPrimary (citable) accession number: P33297
Secondary accession number(s): D6W2H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome