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P33297

- PRS6A_YEAST

UniProt

P33297 - PRS6A_YEAST

Protein

26S protease regulatory subunit 6A

Gene

RPT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi222 – 2298ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. proteasome regulatory particle assembly Source: SGD
    4. ubiquitin-dependent protein catabolic process Source: SGD

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33646-MONOMER.
    ReactomeiREACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189025. ER-Phagosome pathway.
    REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_83036. Orc1 removal from chromatin.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26S protease regulatory subunit 6A
    Alternative name(s):
    Tat-binding protein homolog 1
    Short name:
    TBP-1
    Gene namesi
    Name:RPT5
    Synonyms:YTA1
    Ordered Locus Names:YOR117W
    ORF Names:O3258, YOR3258W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    SGDiS000005643. RPT5.

    Subcellular locationi

    Cytoplasm Curated. Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome regulatory particle, base subcomplex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 43443326S protease regulatory subunit 6APRO_0000084708Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine2 Publications
    Modified residuei180 – 1801Phosphotyrosine1 Publication

    Post-translational modificationi

    N-acetylated by NAT1.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP33297.
    PaxDbiP33297.
    PeptideAtlasiP33297.
    PRIDEiP33297.

    Expressioni

    Gene expression databases

    GenevestigatoriP33297.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSM3P383486EBI-13920,EBI-21152
    NAS2P405556EBI-13920,EBI-14024
    RPN1P387644EBI-13920,EBI-15913
    RPN10P388862EBI-13920,EBI-15949
    RPN14P531964EBI-13920,EBI-23691
    RPT1P332994EBI-13920,EBI-13910
    RPT3P332986EBI-13920,EBI-13905
    RPT4P535497EBI-13920,EBI-18520

    Protein-protein interaction databases

    BioGridi34513. 369 interactions.
    DIPiDIP-1590N.
    IntActiP33297. 38 interactions.
    MINTiMINT-383887.
    STRINGi4932.YOR117W.

    Structurei

    Secondary structure

    1
    434
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi356 – 36510
    Helixi376 – 3816
    Turni382 – 3854
    Helixi388 – 40417
    Beta strandi408 – 4103
    Helixi412 – 42716

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WHKX-ray2.60A/B/C/D/E/F/G/H356-434[»]
    3WHLX-ray4.00A/C/E/G356-434[»]
    4CR2electron microscopy7.70M1-434[»]
    4CR3electron microscopy9.30M1-434[»]
    4CR4electron microscopy8.80M1-434[»]
    ProteinModelPortaliP33297.
    SMRiP33297. Positions 41-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Phylogenomic databases

    eggNOGiCOG1222.
    GeneTreeiENSGT00730000111070.
    HOGENOMiHOG000225143.
    KOiK03065.
    OMAiQKISHEH.
    OrthoDBiEOG7X3R1G.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR005937. 26S_Psome_P45.
    IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLEELDAQ TLPGDDELDQ EILNLSTQEL QTRAKLLDNE IRIFRSELQR    50
    LSHENNVMLE KIKDNKEKIK NNRQLPYLVA NVVEVMDMNE IEDKENSEST 100
    TQGGNVNLDN TAVGKAAVVK TSSRQTVFLP MVGLVDPDKL KPNDLVGVNK 150
    DSYLILDTLP SEFDSRVKAM EVDEKPTETY SDVGGLDKQI EELVEAIVLP 200
    MKRADKFKDM GIRAPKGALM YGPPGTGKTL LARACAAQTN ATFLKLAAPQ 250
    LVQMYIGEGA KLVRDAFALA KEKAPTIIFI DELDAIGTKR FDSEKSGDRE 300
    VQRTMLELLN QLDGFSSDDR VKVLAATNRV DVLDPALLRS GRLDRKIEFP 350
    LPSEDSRAQI LQIHSRKMTT DDDINWQELA RSTDEFNGAQ LKAVTVEAGM 400
    IALRNGQSSV KHEDFVEGIS EVQARKSKSV SFYA 434
    Length:434
    Mass (Da):48,256
    Last modified:January 23, 2007 - v3
    Checksum:iC215422033FCE830
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73569 Genomic DNA. Translation: CAA51971.1.
    X90518 Genomic DNA. Translation: CAA62114.1.
    X94335 Genomic DNA. Translation: CAA64037.1.
    Z75025 Genomic DNA. Translation: CAA99315.1.
    BK006948 Genomic DNA. Translation: DAA10892.1.
    PIRiS46605.
    RefSeqiNP_014760.3. NM_001183536.3.

    Genome annotation databases

    EnsemblFungiiYOR117W; YOR117W; YOR117W.
    GeneIDi854284.
    KEGGisce:YOR117W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73569 Genomic DNA. Translation: CAA51971.1 .
    X90518 Genomic DNA. Translation: CAA62114.1 .
    X94335 Genomic DNA. Translation: CAA64037.1 .
    Z75025 Genomic DNA. Translation: CAA99315.1 .
    BK006948 Genomic DNA. Translation: DAA10892.1 .
    PIRi S46605.
    RefSeqi NP_014760.3. NM_001183536.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WHK X-ray 2.60 A/B/C/D/E/F/G/H 356-434 [» ]
    3WHL X-ray 4.00 A/C/E/G 356-434 [» ]
    4CR2 electron microscopy 7.70 M 1-434 [» ]
    4CR3 electron microscopy 9.30 M 1-434 [» ]
    4CR4 electron microscopy 8.80 M 1-434 [» ]
    ProteinModelPortali P33297.
    SMRi P33297. Positions 41-434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34513. 369 interactions.
    DIPi DIP-1590N.
    IntActi P33297. 38 interactions.
    MINTi MINT-383887.
    STRINGi 4932.YOR117W.

    Proteomic databases

    MaxQBi P33297.
    PaxDbi P33297.
    PeptideAtlasi P33297.
    PRIDEi P33297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR117W ; YOR117W ; YOR117W .
    GeneIDi 854284.
    KEGGi sce:YOR117W.

    Organism-specific databases

    SGDi S000005643. RPT5.

    Phylogenomic databases

    eggNOGi COG1222.
    GeneTreei ENSGT00730000111070.
    HOGENOMi HOG000225143.
    KOi K03065.
    OMAi QKISHEH.
    OrthoDBi EOG7X3R1G.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33646-MONOMER.
    Reactomei REACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189025. ER-Phagosome pathway.
    REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_83036. Orc1 removal from chromatin.

    Miscellaneous databases

    NextBioi 976260.
    PROi P33297.

    Gene expression databases

    Genevestigatori P33297.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR005937. 26S_Psome_P45.
    IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01242. 26Sp45. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
      Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
      Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C836.
    2. "Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames."
      Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W.
      Yeast 12:281-288(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
      Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
      Arch. Biochem. Biophys. 409:341-348(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, ACETYLATION AT ALA-2.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-180, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE 26S PROTEASOME.

    Entry informationi

    Entry nameiPRS6A_YEAST
    AccessioniPrimary (citable) accession number: P33297
    Secondary accession number(s): D6W2H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 133 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3