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P33296

- UBC6_YEAST

UniProt

P33296 - UBC6_YEAST

Protein

Ubiquitin-conjugating enzyme E2 6

Gene

UBC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains.3 PublicationsPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. ubiquitin-protein transferase activity Source: SGD

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. protein monoubiquitination Source: SGD
    3. protein polyubiquitination Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30265-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 6 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein UBC6
    Ubiquitin-protein ligase UBC6
    Gene namesi
    Name:UBC6
    Synonyms:DOA2
    Ordered Locus Names:YER100W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER100w.
    SGDiS000000902. UBC6.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi87 – 871C → S: Loss of activity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250Ubiquitin-conjugating enzyme E2 6PRO_0000082552Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei139 – 1391Phosphoserine1 Publication
    Modified residuei178 – 1781Phosphothreonine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP33296.
    PaxDbiP33296.
    PeptideAtlasiP33296.

    Expressioni

    Gene expression databases

    GenevestigatoriP33296.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399402EBI-19745,EBI-16219

    Protein-protein interaction databases

    BioGridi36846. 88 interactions.
    DIPiDIP-1713N.
    IntActiP33296. 8 interactions.
    MINTiMINT-393639.
    STRINGi4932.YER100W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33296.
    SMRiP33296. Positions 6-160.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 232232CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei233 – 24917HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00530000063379.
    HOGENOMiHOG000170329.
    KOiK04554.
    OMAiFMTGDES.
    OrthoDBiEOG70S7GF.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK    50
    GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP 100
    GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF 150
    KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI 200
    LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK 250
    Length:250
    Mass (Da):28,383
    Last modified:February 1, 1994 - v1
    Checksum:i6D80C35ECC9CF5AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73234 Genomic DNA. Translation: CAA51706.1.
    U18839 Genomic DNA. Translation: AAB64655.1.
    BK006939 Genomic DNA. Translation: DAA07761.1.
    PIRiS36769.
    RefSeqiNP_011026.3. NM_001178991.3.

    Genome annotation databases

    EnsemblFungiiYER100W; YER100W; YER100W.
    GeneIDi856837.
    KEGGisce:YER100W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73234 Genomic DNA. Translation: CAA51706.1 .
    U18839 Genomic DNA. Translation: AAB64655.1 .
    BK006939 Genomic DNA. Translation: DAA07761.1 .
    PIRi S36769.
    RefSeqi NP_011026.3. NM_001178991.3.

    3D structure databases

    ProteinModelPortali P33296.
    SMRi P33296. Positions 6-160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36846. 88 interactions.
    DIPi DIP-1713N.
    IntActi P33296. 8 interactions.
    MINTi MINT-393639.
    STRINGi 4932.YER100W.

    Proteomic databases

    MaxQBi P33296.
    PaxDbi P33296.
    PeptideAtlasi P33296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER100W ; YER100W ; YER100W .
    GeneIDi 856837.
    KEGGi sce:YER100W.

    Organism-specific databases

    CYGDi YER100w.
    SGDi S000000902. UBC6.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00530000063379.
    HOGENOMi HOG000170329.
    KOi K04554.
    OMAi FMTGDES.
    OrthoDBi EOG70S7GF.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-30265-MONOMER.

    Miscellaneous databases

    NextBioi 983144.
    PROi P33296.

    Gene expression databases

    Genevestigatori P33296.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A protein translocation defect linked to ubiquitin conjugation at the endoplasmic reticulum."
      Sommer T., Jentsch S.
      Nature 365:176-179(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor."
      Chen P., Johnson P., Sommer T., Jentsch S., Hochstrasser M.
      Cell 74:357-369(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Degradation signal masking by heterodimerization of MATalpha2 and MATa1 blocks their mutual destruction by the ubiquitin-proteasome pathway."
      Johnson P.R., Swanson R., Rakhilina L., Hochstrasser M.
      Cell 94:217-227(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation."
      Swanson R., Locher M., Hochstrasser M.
      Genes Dev. 15:2660-2674(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Ubx2 links the Cdc48 complex to ER-associated protein degradation."
      Neuber O., Jarosch E., Volkwein C., Walter J., Sommer T.
      Nat. Cell Biol. 7:993-998(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SSM4.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-178, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-178, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBC6_YEAST
    AccessioniPrimary (citable) accession number: P33296
    Secondary accession number(s): D3DM07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3