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Protein

Ubiquitin-conjugating enzyme E2 6

Gene

UBC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains.PROSITE-ProRule annotation3 Publications

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30265-MONOMER
ReactomeiR-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 6 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 6
Ubiquitin carrier protein UBC6
Ubiquitin-protein ligase UBC6
Gene namesi
Name:UBC6
Synonyms:DOA2
Ordered Locus Names:YER100W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER100W
SGDiS000000902 UBC6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 232CytoplasmicSequence analysisAdd BLAST232
Transmembranei233 – 249HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87C → S: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825521 – 250Ubiquitin-conjugating enzyme E2 6Add BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphoserineCombined sources1
Modified residuei178PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33296
PaxDbiP33296
PRIDEiP33296

PTM databases

iPTMnetiP33296

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-19745,EBI-16219

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36846, 163 interactors
DIPiDIP-1713N
IntActiP33296, 27 interactors
MINTiP33296
STRINGi4932.YER100W

Structurei

3D structure databases

ProteinModelPortaliP33296
SMRiP33296
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063379
HOGENOMiHOG000170329
InParanoidiP33296
KOiK04554
OMAiFQPPSIY
OrthoDBiEOG092C4P07

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK
60 70 80 90 100
GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP
110 120 130 140 150
GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF
160 170 180 190 200
KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI
210 220 230 240 250
LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK
Length:250
Mass (Da):28,383
Last modified:February 1, 1994 - v1
Checksum:i6D80C35ECC9CF5AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73234 Genomic DNA Translation: CAA51706.1
U18839 Genomic DNA Translation: AAB64655.1
BK006939 Genomic DNA Translation: DAA07761.1
PIRiS36769
RefSeqiNP_011026.3, NM_001178991.3

Genome annotation databases

EnsemblFungiiYER100W; YER100W; YER100W
GeneIDi856837
KEGGisce:YER100W

Similar proteinsi

Entry informationi

Entry nameiUBC6_YEAST
AccessioniPrimary (citable) accession number: P33296
Secondary accession number(s): D3DM07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health