Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 6

Gene

UBC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains.PROSITE-ProRule annotation3 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediate

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30265-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 6 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 6
Ubiquitin carrier protein UBC6
Ubiquitin-protein ligase UBC6
Gene namesi
Name:UBC6
Synonyms:DOA2
Ordered Locus Names:YER100W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER100W.
SGDiS000000902. UBC6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 232232CytoplasmicSequence analysisAdd
BLAST
Transmembranei233 – 24917HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi87 – 871C → S: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Ubiquitin-conjugating enzyme E2 6PRO_0000082552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391PhosphoserineCombined sources
Modified residuei178 – 1781PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33296.

PTM databases

iPTMnetiP33296.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-19745,EBI-16219

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36846. 88 interactions.
DIPiDIP-1713N.
IntActiP33296. 8 interactions.
MINTiMINT-393639.

Structurei

3D structure databases

ProteinModelPortaliP33296.
SMRiP33296. Positions 6-160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063379.
HOGENOMiHOG000170329.
InParanoidiP33296.
KOiK04554.
OMAiYILARPN.
OrthoDBiEOG092C4P07.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK
60 70 80 90 100
GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP
110 120 130 140 150
GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF
160 170 180 190 200
KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI
210 220 230 240 250
LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK
Length:250
Mass (Da):28,383
Last modified:February 1, 1994 - v1
Checksum:i6D80C35ECC9CF5AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73234 Genomic DNA. Translation: CAA51706.1.
U18839 Genomic DNA. Translation: AAB64655.1.
BK006939 Genomic DNA. Translation: DAA07761.1.
PIRiS36769.
RefSeqiNP_011026.3. NM_001178991.3.

Genome annotation databases

EnsemblFungiiYER100W; YER100W; YER100W.
GeneIDi856837.
KEGGisce:YER100W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73234 Genomic DNA. Translation: CAA51706.1.
U18839 Genomic DNA. Translation: AAB64655.1.
BK006939 Genomic DNA. Translation: DAA07761.1.
PIRiS36769.
RefSeqiNP_011026.3. NM_001178991.3.

3D structure databases

ProteinModelPortaliP33296.
SMRiP33296. Positions 6-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36846. 88 interactions.
DIPiDIP-1713N.
IntActiP33296. 8 interactions.
MINTiMINT-393639.

PTM databases

iPTMnetiP33296.

Proteomic databases

MaxQBiP33296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER100W; YER100W; YER100W.
GeneIDi856837.
KEGGisce:YER100W.

Organism-specific databases

EuPathDBiFungiDB:YER100W.
SGDiS000000902. UBC6.

Phylogenomic databases

GeneTreeiENSGT00530000063379.
HOGENOMiHOG000170329.
InParanoidiP33296.
KOiK04554.
OMAiYILARPN.
OrthoDBiEOG092C4P07.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-30265-MONOMER.

Miscellaneous databases

PROiP33296.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC6_YEAST
AccessioniPrimary (citable) accession number: P33296
Secondary accession number(s): D3DM07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.