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P33287

- PDC_NEUCR

UniProt

P33287 - PDC_NEUCR

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Protein
Pyruvate decarboxylase
Gene
cfp, pdc-1, NCU02193
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 2-oxo acid = an aldehyde + CO2.

Cofactori

Binds 1 metal ion per subunit.
Binds 1 thiamine pyrophosphate per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331Substrate By similarity
Binding sitei120 – 1201Substrate By similarity
Metal bindingi444 – 4441Magnesium By similarity
Metal bindingi471 – 4711Magnesium By similarity
Metal bindingi473 – 4731Magnesium; via carbonyl oxygen By similarity
Binding sitei477 – 4771Substrate By similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. magnesium ion binding Source: InterPro
  3. pyruvate decarboxylase activity Source: UniProtKB-EC
  4. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. pyruvate metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00231.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate decarboxylase (EC:4.1.1.1)
Alternative name(s):
8-10 nm cytoplasmic filament-associated protein
P59NC
Gene namesi
Name:cfp
Synonyms:pdc-1
ORF Names:NCU02193
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 7, Linkage Group VII

Subcellular locationi

Cytoplasm
Note: Cytoplasmic filaments.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 22Removed in mature form
PRO_0000035653
Chaini3 – 570568Pyruvate decarboxylase
PRO_0000035654Add
BLAST

Interactioni

Subunit structurei

Homopolymer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-7533403,EBI-7533403

Protein-protein interaction databases

MINTiMINT-8291074.

Structurei

3D structure databases

ProteinModelPortaliP33287.
SMRiP33287. Positions 9-565.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni394 – 47683Thiamine pyrophosphate binding
Add
BLAST

Sequence similaritiesi

Belongs to the TPP enzyme family.

Phylogenomic databases

eggNOGiCOG3961.
HOGENOMiHOG000061335.
KOiK01568.
OMAiYIEIPTN.
OrthoDBiEOG7K6Q3N.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR012110. TPP_enzyme.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036565. Pyruvt_ip_decrb. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33287-1 [UniParc]FASTAAdd to Basket

« Hide

MVAQQQGKFT VGDYLAERLA QVGVRHHFVV PGDYNLILLD KLQAHPDLKE    50
VGCANELNCS LAAEGYARAN GISACVVTYS VGALSAFNGT GSAYAENLPL 100
VLISGSPNTN DPSQYHILHH TLGHPDYTYQ YEMAKKITCC AVAIPRAIDA 150
PRLIDRALRA AILARKPCYI EIPTNLAGAT CVRPGPISAI TDPITSDKSA 200
LEAAAKCAAE YLDGKLKPVI LVGPKAGRAG SEKELIEFAE AMGCAVALQP 250
AAKGMFPEDH KQFVGIFWGQ VSSDAADAMV HWADAMICVG AVFNDYSTVG 300
WTAVPNIPLM TVDMDHVTFP GAHFSRVRMC EFLSHLATQV TFNDSTMIEY 350
KRLKPDPPHV HTAEREEPLS RKEISRQVQE MLTDKTSLFV DTGDSWFNGI 400
QLKLPPGAKF EIEMQWGHIG WSIPAAFGYA LRHPDRHTIV LVGDGSFQVT 450
AQEVSQMVRF KVPITIMLIN NRGYTIEVEI HDGSYNKIKN WDYAMLVEAF 500
NSTDGHAKGL LANTAGELAD AIKVAESHKE GPTLIECTID QDDCSKELIT 550
WGHYVAAANA RPPRNMSVQE 570
Length:570
Mass (Da):62,263
Last modified:February 1, 1994 - v1
Checksum:iDF48B86C24EDAACF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L09125 mRNA. Translation: AAA33567.1.
U65927 Genomic DNA. Translation: AAB17969.1.
CM002242 Genomic DNA. Translation: ESA42032.1.
S40298 mRNA. Translation: AAB22524.2.
PIRiJN0782.
RefSeqiXP_959587.1. XM_954494.2.
UniGeneiNcr.14819.

Genome annotation databases

EnsemblFungiiEFNCRT00000003234; EFNCRP00000003234; EFNCRG00000003230.
GeneIDi3875734.
KEGGincr:NCU02193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L09125 mRNA. Translation: AAA33567.1 .
U65927 Genomic DNA. Translation: AAB17969.1 .
CM002242 Genomic DNA. Translation: ESA42032.1 .
S40298 mRNA. Translation: AAB22524.2 .
PIRi JN0782.
RefSeqi XP_959587.1. XM_954494.2.
UniGenei Ncr.14819.

3D structure databases

ProteinModelPortali P33287.
SMRi P33287. Positions 9-565.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8291074.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000003234 ; EFNCRP00000003234 ; EFNCRG00000003230 .
GeneIDi 3875734.
KEGGi ncr:NCU02193.

Phylogenomic databases

eggNOGi COG3961.
HOGENOMi HOG000061335.
KOi K01568.
OMAi YIEIPTN.
OrthoDBi EOG7K6Q3N.

Enzyme and pathway databases

UniPathwayi UPA00231 .

Family and domain databases

Gene3Di 3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProi IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR012110. TPP_enzyme.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF036565. Pyruvt_ip_decrb. 1 hit.
SUPFAMi SSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
PROSITEi PS00187. TPP_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 59-kDa polypeptide constituent of 8-10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase."
    Alvarez M.E., Rosa A.L., Temporini E.D., Wolstenholme A., Panzetta G., Patrito L., Maccioni H.J.F.
    Gene 130:253-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-25.
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. Temporini E.D., Alvarez M.E., Mautino M., Kawaguchi T., Kinghorn J., Rosa A.L.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Mycelium.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa."
    Haedo S.D., Temporini E.D., Alvarez M.E., Maccioni H.J., Rosa A.L.
    Genetics 131:575-580(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.

Entry informationi

Entry nameiPDC_NEUCR
AccessioniPrimary (citable) accession number: P33287
Secondary accession number(s): Q7RV97, V5IKN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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