P33287 (PDC_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate decarboxylase
EC=4.1.1.1

Alternative name(s):
8-10 nm cytoplasmic filament-associated protein
P59NC

Gene names

Name:	cfp
ORF Names:	NCU02193

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A 2-oxo acid = an aldehyde + CO2.
Cofactor	Binds 1 metal ion per subunit. Binds 1 thiamine pyrophosphate per subunit.
Pathway	Carbohydrate metabolism; pyruvate metabolism.
Subunit structure	Homopolymer.
Subcellular location	Cytoplasm. Note: Cytoplasmic filaments.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	pyruvate metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro pyruvate decarboxylase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 2	2	Removed in mature form		PRO_0000035653
	Chain	3 – 570	568	Pyruvate decarboxylase		PRO_0000035654
Regions
	Region	394 – 476	83	Thiamine pyrophosphate binding
Sites
	Metal binding	444	1	Magnesium By similarity
	Metal binding	471	1	Magnesium By similarity
	Metal binding	473	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	477	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33287 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: DF48B86C24EDAACF	FASTA	570	62,263
	10 20 30 40 50 60 MVAQQQGKFT VGDYLAERLA QVGVRHHFVV PGDYNLILLD KLQAHPDLKE VGCANELNCS 70 80 90 100 110 120 LAAEGYARAN GISACVVTYS VGALSAFNGT GSAYAENLPL VLISGSPNTN DPSQYHILHH 130 140 150 160 170 180 TLGHPDYTYQ YEMAKKITCC AVAIPRAIDA PRLIDRALRA AILARKPCYI EIPTNLAGAT 190 200 210 220 230 240 CVRPGPISAI TDPITSDKSA LEAAAKCAAE YLDGKLKPVI LVGPKAGRAG SEKELIEFAE 250 260 270 280 290 300 AMGCAVALQP AAKGMFPEDH KQFVGIFWGQ VSSDAADAMV HWADAMICVG AVFNDYSTVG 310 320 330 340 350 360 WTAVPNIPLM TVDMDHVTFP GAHFSRVRMC EFLSHLATQV TFNDSTMIEY KRLKPDPPHV 370 380 390 400 410 420 HTAEREEPLS RKEISRQVQE MLTDKTSLFV DTGDSWFNGI QLKLPPGAKF EIEMQWGHIG 430 440 450 460 470 480 WSIPAAFGYA LRHPDRHTIV LVGDGSFQVT AQEVSQMVRF KVPITIMLIN NRGYTIEVEI 490 500 510 520 530 540 HDGSYNKIKN WDYAMLVEAF NSTDGHAKGL LANTAGELAD AIKVAESHKE GPTLIECTID 550 560 570 QDDCSKELIT WGHYVAAANA RPPRNMSVQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 59-kDa polypeptide constituent of 8-10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase." Alvarez M.E., Rosa A.L., Temporini E.D., Wolstenholme A., Panzetta G., Patrito L., Maccioni H.J.F. Gene 130:253-258(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-25. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	Temporini E.D., Alvarez M.E., Mautino M., Kawaguchi T., Kinghorn J., Rosa A.L. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Mycelium.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]	"Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa." Haedo S.D., Temporini E.D., Alvarez M.E., Maccioni H.J., Rosa A.L. Genetics 131:575-580(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09125 mRNA. Translation: AAA33567.1. U65927 Genomic DNA. Translation: AAB17969.1. AABX02000010 Genomic DNA. Translation: EAA30351.1. S40298 mRNA. Translation: AAB22524.2.
PIR	JN0782.
RefSeq	XP_959587.1. XM_954494.2.
UniGene	Ncr.14819.
3D structure databases
ProteinModelPortal	P33287.
SMR	P33287. Positions 9-565.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003234; EFNCRP00000003234; EFNCRG00000003230.
GeneID	3875734.
KEGG	ncr:NCU02193.
Phylogenomic databases
eggNOG	COG3961.
HOGENOM	HOG000061335.
OMA	FIEVIAH.
OrthoDB	EOG444PTS.
Enzyme and pathway databases
UniPathway	UPA00231.
Family and domain databases
InterPro	IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR012110. TPP_enzyme. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF4. PTHR18968:SF4. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PIRSF	PIRSF036565. Pyruvt_ip_decrb. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDC_NEUCR

Accession

Primary (citable) accession number: P33287
Secondary accession number(s): Q7RV97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program