Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P33287 (PDC_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate decarboxylase

EC=4.1.1.1
Alternative name(s):
8-10 nm cytoplasmic filament-associated protein
P59NC
Gene names
Name:cfp
Synonyms:pdc-1
ORF Names:NCU02193
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A 2-oxo acid = an aldehyde + CO2.

Cofactor

Binds 1 metal ion per subunit.

Binds 1 thiamine pyrophosphate per subunit.

Pathway

Carbohydrate metabolism; pyruvate metabolism.

Subunit structure

Homopolymer.

Subcellular location

Cytoplasm. Note: Cytoplasmic filaments.

Sequence similarities

Belongs to the TPP enzyme family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself5EBI-7533403,EBI-7533403

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 22Removed in mature form
PRO_0000035653
Chain3 – 570568Pyruvate decarboxylase
PRO_0000035654

Regions

Region394 – 47683Thiamine pyrophosphate binding

Sites

Metal binding4441Magnesium By similarity
Metal binding4711Magnesium By similarity
Metal binding4731Magnesium; via carbonyl oxygen By similarity
Binding site331Substrate By similarity
Binding site1201Substrate By similarity
Binding site4771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P33287 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: DF48B86C24EDAACF

FASTA57062,263
        10         20         30         40         50         60 
MVAQQQGKFT VGDYLAERLA QVGVRHHFVV PGDYNLILLD KLQAHPDLKE VGCANELNCS 

        70         80         90        100        110        120 
LAAEGYARAN GISACVVTYS VGALSAFNGT GSAYAENLPL VLISGSPNTN DPSQYHILHH 

       130        140        150        160        170        180 
TLGHPDYTYQ YEMAKKITCC AVAIPRAIDA PRLIDRALRA AILARKPCYI EIPTNLAGAT 

       190        200        210        220        230        240 
CVRPGPISAI TDPITSDKSA LEAAAKCAAE YLDGKLKPVI LVGPKAGRAG SEKELIEFAE 

       250        260        270        280        290        300 
AMGCAVALQP AAKGMFPEDH KQFVGIFWGQ VSSDAADAMV HWADAMICVG AVFNDYSTVG 

       310        320        330        340        350        360 
WTAVPNIPLM TVDMDHVTFP GAHFSRVRMC EFLSHLATQV TFNDSTMIEY KRLKPDPPHV 

       370        380        390        400        410        420 
HTAEREEPLS RKEISRQVQE MLTDKTSLFV DTGDSWFNGI QLKLPPGAKF EIEMQWGHIG 

       430        440        450        460        470        480 
WSIPAAFGYA LRHPDRHTIV LVGDGSFQVT AQEVSQMVRF KVPITIMLIN NRGYTIEVEI 

       490        500        510        520        530        540 
HDGSYNKIKN WDYAMLVEAF NSTDGHAKGL LANTAGELAD AIKVAESHKE GPTLIECTID 

       550        560        570 
QDDCSKELIT WGHYVAAANA RPPRNMSVQE 

« Hide

References

« Hide 'large scale' references
[1]"The 59-kDa polypeptide constituent of 8-10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase."
Alvarez M.E., Rosa A.L., Temporini E.D., Wolstenholme A., Panzetta G., Patrito L., Maccioni H.J.F.
Gene 130:253-258(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-25.
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]Temporini E.D., Alvarez M.E., Mautino M., Kawaguchi T., Kinghorn J., Rosa A.L.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Mycelium.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa."
Haedo S.D., Temporini E.D., Alvarez M.E., Maccioni H.J., Rosa A.L.
Genetics 131:575-580(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L09125 mRNA. Translation: AAA33567.1.
U65927 Genomic DNA. Translation: AAB17969.1.
CM002242 Genomic DNA. Translation: ESA42032.1.
S40298 mRNA. Translation: AAB22524.2.
PIRJN0782.
RefSeqXP_959587.1. XM_954494.2.
UniGeneNcr.14819.

3D structure databases

ProteinModelPortalP33287.
SMRP33287. Positions 9-565.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8291074.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003234; EFNCRP00000003234; EFNCRG00000003230.
GeneID3875734.
KEGGncr:NCU02193.

Phylogenomic databases

eggNOGCOG3961.
HOGENOMHOG000061335.
KOK01568.
OMAHIGWSIP.
OrthoDBEOG7K6Q3N.

Enzyme and pathway databases

UniPathwayUPA00231.

Family and domain databases

InterProIPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR012110. TPP_enzyme.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PANTHERPTHR18968:SF4. PTHR18968:SF4. 1 hit.
PfamPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF036565. Pyruvt_ip_decrb. 1 hit.
PROSITEPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDC_NEUCR
AccessionPrimary (citable) accession number: P33287
Secondary accession number(s): Q7RV97, V5IKN9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways