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P33287

- PDC_NEUCR

UniProt

P33287 - PDC_NEUCR

Protein

Pyruvate decarboxylase

Gene

cfp

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A 2-oxo acid = an aldehyde + CO2.

    Cofactori

    Binds 1 metal ion per subunit.
    Binds 1 thiamine pyrophosphate per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Metal bindingi444 – 4441MagnesiumBy similarity
    Metal bindingi471 – 4711MagnesiumBy similarity
    Metal bindingi473 – 4731Magnesium; via carbonyl oxygenBy similarity
    Binding sitei477 – 4771SubstrateBy similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. magnesium ion binding Source: InterPro
    3. pyruvate decarboxylase activity Source: UniProtKB-EC
    4. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. pyruvate metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    UniPathwayiUPA00231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate decarboxylase (EC:4.1.1.1)
    Alternative name(s):
    8-10 nm cytoplasmic filament-associated protein
    P59NC
    Gene namesi
    Name:cfp
    Synonyms:pdc-1
    ORF Names:NCU02193
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 7, Linkage Group VII

    Subcellular locationi

    Cytoplasm
    Note: Cytoplasmic filaments.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 22Removed in mature form1 PublicationPRO_0000035653
    Chaini3 – 570568Pyruvate decarboxylasePRO_0000035654Add
    BLAST

    Interactioni

    Subunit structurei

    Homopolymer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-7533403,EBI-7533403

    Protein-protein interaction databases

    MINTiMINT-8291074.

    Structurei

    3D structure databases

    ProteinModelPortaliP33287.
    SMRiP33287. Positions 9-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni394 – 47683Thiamine pyrophosphate bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Phylogenomic databases

    eggNOGiCOG3961.
    HOGENOMiHOG000061335.
    KOiK01568.
    OMAiYIEIPTN.
    OrthoDBiEOG7K6Q3N.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR012110. TPP_enzyme.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036565. Pyruvt_ip_decrb. 1 hit.
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    PROSITEiPS00187. TPP_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33287-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAQQQGKFT VGDYLAERLA QVGVRHHFVV PGDYNLILLD KLQAHPDLKE    50
    VGCANELNCS LAAEGYARAN GISACVVTYS VGALSAFNGT GSAYAENLPL 100
    VLISGSPNTN DPSQYHILHH TLGHPDYTYQ YEMAKKITCC AVAIPRAIDA 150
    PRLIDRALRA AILARKPCYI EIPTNLAGAT CVRPGPISAI TDPITSDKSA 200
    LEAAAKCAAE YLDGKLKPVI LVGPKAGRAG SEKELIEFAE AMGCAVALQP 250
    AAKGMFPEDH KQFVGIFWGQ VSSDAADAMV HWADAMICVG AVFNDYSTVG 300
    WTAVPNIPLM TVDMDHVTFP GAHFSRVRMC EFLSHLATQV TFNDSTMIEY 350
    KRLKPDPPHV HTAEREEPLS RKEISRQVQE MLTDKTSLFV DTGDSWFNGI 400
    QLKLPPGAKF EIEMQWGHIG WSIPAAFGYA LRHPDRHTIV LVGDGSFQVT 450
    AQEVSQMVRF KVPITIMLIN NRGYTIEVEI HDGSYNKIKN WDYAMLVEAF 500
    NSTDGHAKGL LANTAGELAD AIKVAESHKE GPTLIECTID QDDCSKELIT 550
    WGHYVAAANA RPPRNMSVQE 570
    Length:570
    Mass (Da):62,263
    Last modified:February 1, 1994 - v1
    Checksum:iDF48B86C24EDAACF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09125 mRNA. Translation: AAA33567.1.
    U65927 Genomic DNA. Translation: AAB17969.1.
    CM002242 Genomic DNA. Translation: ESA42032.1.
    S40298 mRNA. Translation: AAB22524.2.
    PIRiJN0782.
    RefSeqiXP_959587.1. XM_954494.2.
    UniGeneiNcr.14819.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000003234; EFNCRP00000003234; EFNCRG00000003230.
    GeneIDi3875734.
    KEGGincr:NCU02193.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09125 mRNA. Translation: AAA33567.1 .
    U65927 Genomic DNA. Translation: AAB17969.1 .
    CM002242 Genomic DNA. Translation: ESA42032.1 .
    S40298 mRNA. Translation: AAB22524.2 .
    PIRi JN0782.
    RefSeqi XP_959587.1. XM_954494.2.
    UniGenei Ncr.14819.

    3D structure databases

    ProteinModelPortali P33287.
    SMRi P33287. Positions 9-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8291074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000003234 ; EFNCRP00000003234 ; EFNCRG00000003230 .
    GeneIDi 3875734.
    KEGGi ncr:NCU02193.

    Phylogenomic databases

    eggNOGi COG3961.
    HOGENOMi HOG000061335.
    KOi K01568.
    OMAi YIEIPTN.
    OrthoDBi EOG7K6Q3N.

    Enzyme and pathway databases

    UniPathwayi UPA00231 .

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR012110. TPP_enzyme.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036565. Pyruvt_ip_decrb. 1 hit.
    SUPFAMi SSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    PROSITEi PS00187. TPP_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 59-kDa polypeptide constituent of 8-10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase."
      Alvarez M.E., Rosa A.L., Temporini E.D., Wolstenholme A., Panzetta G., Patrito L., Maccioni H.J.F.
      Gene 130:253-258(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-25.
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. Temporini E.D., Alvarez M.E., Mautino M., Kawaguchi T., Kinghorn J., Rosa A.L.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Mycelium.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa."
      Haedo S.D., Temporini E.D., Alvarez M.E., Maccioni H.J., Rosa A.L.
      Genetics 131:575-580(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.

    Entry informationi

    Entry nameiPDC_NEUCR
    AccessioniPrimary (citable) accession number: P33287
    Secondary accession number(s): Q7RV97, V5IKN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3