Reviewed, UniProtKB/Swiss-Prot P33284 (HXK_KLULA)

Names and origin

Protein names

Recommended name:
    Hexokinase
    EC=2.7.1.1

Gene names

Name:	RAG5
Ordered Locus Names:	KLLA0D11352g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer and homodimer. The monomeric form is active, the homodimeric form inactive. Ref.3
Sequence similarities	Belongs to the hexokinase family.
Mass spectrometry	Molecular mass is 53476±5 Da from positions 2 - 485. Determined by ESI. Ref.3

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 485	484	Hexokinase		PRO_0000197608
Regions
	Region	151 – 177	27	Glucose-binding Potential
Sites
	Binding site	111	1	ATP By similarity
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	206	1	A → R in CAA43855. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33284-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3E93D80C92663FB0	FASTA	485	53,610
	10 20 30 40 50 60 MVRLGPKKPP ARKGSMADVP ANLMEQIHGL ETLFTVSSEK MRSIVKHFIS ELDKGLSKKG 70 80 90 100 110 120 GNIPMIPGWV VEYPTGKETG DFLALDLGGT NLRVVLVKLG GNHDFDTTQN KYRLPDHLRT 130 140 150 160 170 180 GTSEQLWSFI AKCLKEFVDE WYPDGVSEPL PLGFTFSYPA SQKKINSGVL QRWTKGFDIE 190 200 210 220 230 240 GVEGHDVVPM LQEQIEKLNI PINVVALIND TTGTLVASLY TDPQTKMGII IGTGVNGAYY 250 260 270 280 290 300 DVVSGIEKLE GLLPEDIGPD SPMAINCEYG SFDNEHLVLP RTKYDVIIDE ESPRPGQQAF 310 320 330 340 350 360 EKMTSGYYLG EIMRLVLLDL YDSGFIFKDQ DISKLKEAYV MDTSYPSKIE DDPFENLEDT 370 380 390 400 410 420 DDLFKTNLNI ETTVVERKLI RKLAELVGTR AARLTVCGVS AICDKRGYKT AHIAADGSVF 430 440 450 460 470 480 NRYPGYKEKA AQALKDIYNW DVEKMEDHPI QLVAAEDGSG VGAAIIACLT QKRLAAGKSV GIKGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis." Prior C., Mamessier P., Fukuhara H., Chen X.J., Wesolowski-Louvel M. Mol. Cell. Biol. 13:3882-3889(1993) [PubMed: 8321195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76492 / CBS 2359/152 / CLIB 210 and PM6-7A.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.
[3]	"The unique hexokinase of Kluyveromyces lactis. Molecular and functional characterization and evaluation of a role in glucose signaling." Baer D., Golbik R., Huebner G., Lilie H., Mueller E.-C., Naumann M., Otto A., Reuter R., Breunig K.D., Kriegel T.M. J. Biol. Chem. 278:39280-39286(2003) [PubMed: 12882981] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21; 136-163 AND 198-226, MASS SPECTROMETRY, SUBUNIT, PHOSPHORYLATION AT SER-15. Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61680 Genomic DNA. Translation: CAA43855.1. CR382124 Genomic DNA. Translation: CAH00663.1.
PIR	A48132.
RefSeq	XP_453567.1.
3D structure databases
HSSP	HSSP built from PDB template 1IG8 based on UniProtKB P04807.
SMR	P33284. Positions 18-484.
ModBase	Search...
Protein-protein interaction databases
STRING	P33284.
Genome annotation databases
GeneID	2893280.
GenomeReviews	Gene locus KLLA0D11352g in contig CR382124_GR.
KEGG	kla:KLLA0D11352g.
Phylogenomic databases
HOGENOM	P33284.
OMA	YGAFDNE.
Enzyme and pathway databases
BRENDA	2.7.1.1. 74088.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK_KLULA

Accession

Primary (citable) accession number: P33284

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)