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P33284

- HXK_KLULA

UniProt

P33284 - HXK_KLULA

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Protein
Hexokinase
Gene
RAG5, KLLA0D11352g
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hexokinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
  2. hexose metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.1. 2825.
SABIO-RKiP33284.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase (EC:2.7.1.1)
Gene namesi
Name:RAG5
Ordered Locus Names:KLLA0D11352g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome D

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 485484Hexokinase
PRO_0000197608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer and homodimer. The monomeric form is active, the homodimeric form inactive.1 Publication

Protein-protein interaction databases

STRINGi28985.P33284.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 3414
Helixi38 – 5619
Beta strandi57 – 593
Beta strandi61 – 633
Beta strandi79 – 10325
Beta strandi105 – 1139
Helixi118 – 1203
Helixi123 – 14119
Beta strandi150 – 1567
Beta strandi158 – 1614
Beta strandi163 – 1664
Helixi187 – 19711
Beta strandi202 – 2087
Helixi210 – 22112
Beta strandi225 – 24218
Helixi243 – 2453
Helixi247 – 2493
Turni250 – 2523
Beta strandi262 – 2665
Helixi269 – 2713
Turni272 – 2754
Beta strandi277 – 2793
Helixi283 – 2919
Beta strandi292 – 2943
Helixi299 – 3046
Turni306 – 3083
Helixi309 – 32214
Beta strandi325 – 3273
Helixi333 – 3364
Helixi344 – 3518
Helixi358 – 36811
Helixi374 – 40633
Beta strandi409 – 4179
Helixi418 – 4225
Helixi426 – 43813
Helixi445 – 4473
Beta strandi449 – 4546
Turni458 – 4603
Helixi461 – 47616
Beta strandi480 – 4823

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O08X-ray2.00A/B1-485[»]
3O1BX-ray2.80A1-485[»]
3O1WX-ray1.66A/B1-485[»]
3O4WX-ray1.61A/B1-485[»]
3O5BX-ray1.97A/B1-485[»]
3O6WX-ray1.48A/B1-485[»]
3O80X-ray2.18A1-485[»]
3O8MX-ray1.42A1-485[»]
4JAXX-ray2.26A/B/C/D/E/F1-485[»]
ProteinModelPortaliP33284.
SMRiP33284. Positions 18-484.

Miscellaneous databases

EvolutionaryTraceiP33284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 222201Hexokinase type-1
Add
BLAST
Domaini224 – 472249Hexokinase type-2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 17727Glucose-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162670.
KOiK00844.
OMAiARISACG.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33284-1 [UniParc]FASTAAdd to Basket

« Hide

MVRLGPKKPP ARKGSMADVP ANLMEQIHGL ETLFTVSSEK MRSIVKHFIS    50
ELDKGLSKKG GNIPMIPGWV VEYPTGKETG DFLALDLGGT NLRVVLVKLG 100
GNHDFDTTQN KYRLPDHLRT GTSEQLWSFI AKCLKEFVDE WYPDGVSEPL 150
PLGFTFSYPA SQKKINSGVL QRWTKGFDIE GVEGHDVVPM LQEQIEKLNI 200
PINVVALIND TTGTLVASLY TDPQTKMGII IGTGVNGAYY DVVSGIEKLE 250
GLLPEDIGPD SPMAINCEYG SFDNEHLVLP RTKYDVIIDE ESPRPGQQAF 300
EKMTSGYYLG EIMRLVLLDL YDSGFIFKDQ DISKLKEAYV MDTSYPSKIE 350
DDPFENLEDT DDLFKTNLNI ETTVVERKLI RKLAELVGTR AARLTVCGVS 400
AICDKRGYKT AHIAADGSVF NRYPGYKEKA AQALKDIYNW DVEKMEDHPI 450
QLVAAEDGSG VGAAIIACLT QKRLAAGKSV GIKGE 485
Length:485
Mass (Da):53,610
Last modified:January 23, 2007 - v3
Checksum:i3E93D80C92663FB0
GO

Mass spectrometryi

Molecular mass is 53476±5 Da from positions 2 - 485. Determined by ESI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti206 – 2061A → R in CAA43855. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61680 Genomic DNA. Translation: CAA43855.1.
CR382124 Genomic DNA. Translation: CAH00663.1.
PIRiA48132.
RefSeqiXP_453567.1. XM_453567.1.

Genome annotation databases

GeneIDi2893280.
KEGGikla:KLLA0D11352g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61680 Genomic DNA. Translation: CAA43855.1 .
CR382124 Genomic DNA. Translation: CAH00663.1 .
PIRi A48132.
RefSeqi XP_453567.1. XM_453567.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3O08 X-ray 2.00 A/B 1-485 [» ]
3O1B X-ray 2.80 A 1-485 [» ]
3O1W X-ray 1.66 A/B 1-485 [» ]
3O4W X-ray 1.61 A/B 1-485 [» ]
3O5B X-ray 1.97 A/B 1-485 [» ]
3O6W X-ray 1.48 A/B 1-485 [» ]
3O80 X-ray 2.18 A 1-485 [» ]
3O8M X-ray 1.42 A 1-485 [» ]
4JAX X-ray 2.26 A/B/C/D/E/F 1-485 [» ]
ProteinModelPortali P33284.
SMRi P33284. Positions 18-484.
ModBasei Search...

Protein-protein interaction databases

STRINGi 28985.P33284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2893280.
KEGGi kla:KLLA0D11352g.

Phylogenomic databases

eggNOGi COG5026.
HOGENOMi HOG000162670.
KOi K00844.
OMAi ARISACG.
OrthoDBi EOG79SF68.

Enzyme and pathway databases

UniPathwayi UPA00242 .
BRENDAi 2.7.1.1. 2825.
SABIO-RKi P33284.

Miscellaneous databases

EvolutionaryTracei P33284.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis."
    Prior C., Mamessier P., Fukuhara H., Chen X.J., Wesolowski-Louvel M.
    Mol. Cell. Biol. 13:3882-3889(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 76492 / CBS 2359/152 / CLIB 210 and PM6-7A.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  3. "The unique hexokinase of Kluyveromyces lactis. Molecular and functional characterization and evaluation of a role in glucose signaling."
    Baer D., Golbik R., Huebner G., Lilie H., Mueller E.-C., Naumann M., Otto A., Reuter R., Breunig K.D., Kriegel T.M.
    J. Biol. Chem. 278:39280-39286(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21; 136-163 AND 198-226, MASS SPECTROMETRY, SUBUNIT, PHOSPHORYLATION AT SER-15.
    Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.

Entry informationi

Entry nameiHXK_KLULA
AccessioniPrimary (citable) accession number: P33284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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