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Protein

Hexokinase

Gene

RAG5

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.1. 2825.
SABIO-RKP33284.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase (EC:2.7.1.1)
Gene namesi
Name:RAG5
Ordered Locus Names:KLLA0D11352g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001976082 – 485HexokinaseAdd BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP33284.

Interactioni

Subunit structurei

Monomer and homodimer. The monomeric form is active, the homodimeric form inactive.1 Publication

Protein-protein interaction databases

STRINGi284590.XP_453567.1.

Structurei

Secondary structure

1485
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 34Combined sources14
Helixi38 – 56Combined sources19
Beta strandi57 – 59Combined sources3
Beta strandi61 – 63Combined sources3
Beta strandi79 – 103Combined sources25
Beta strandi105 – 113Combined sources9
Helixi118 – 120Combined sources3
Helixi123 – 141Combined sources19
Beta strandi150 – 156Combined sources7
Beta strandi158 – 161Combined sources4
Beta strandi163 – 166Combined sources4
Helixi187 – 197Combined sources11
Beta strandi202 – 208Combined sources7
Helixi210 – 221Combined sources12
Beta strandi225 – 242Combined sources18
Helixi243 – 245Combined sources3
Helixi247 – 249Combined sources3
Turni250 – 252Combined sources3
Beta strandi262 – 266Combined sources5
Helixi269 – 271Combined sources3
Turni272 – 275Combined sources4
Beta strandi277 – 279Combined sources3
Helixi283 – 291Combined sources9
Beta strandi292 – 294Combined sources3
Helixi299 – 304Combined sources6
Turni306 – 308Combined sources3
Helixi309 – 322Combined sources14
Beta strandi325 – 327Combined sources3
Helixi333 – 336Combined sources4
Helixi344 – 351Combined sources8
Helixi358 – 368Combined sources11
Helixi374 – 406Combined sources33
Beta strandi409 – 417Combined sources9
Helixi418 – 422Combined sources5
Helixi426 – 438Combined sources13
Helixi445 – 447Combined sources3
Beta strandi449 – 454Combined sources6
Turni458 – 460Combined sources3
Helixi461 – 476Combined sources16
Beta strandi480 – 482Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3O08X-ray2.00A/B1-485[»]
3O1BX-ray2.80A1-485[»]
3O1WX-ray1.66A/B1-485[»]
3O4WX-ray1.61A/B1-485[»]
3O5BX-ray1.97A/B1-485[»]
3O6WX-ray1.48A/B1-485[»]
3O80X-ray2.18A1-485[»]
3O8MX-ray1.42A1-485[»]
4JAXX-ray2.26A/B/C/D/E/F1-485[»]
ProteinModelPortaliP33284.
SMRiP33284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 468HexokinasePROSITE-ProRule annotationAdd BLAST448

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 208Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST134
Regioni151 – 177Glucose-bindingSequence analysisAdd BLAST27
Regioni209 – 457Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
HOGENOMiHOG000162670.
InParanoidiP33284.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLGPKKPP ARKGSMADVP ANLMEQIHGL ETLFTVSSEK MRSIVKHFIS
60 70 80 90 100
ELDKGLSKKG GNIPMIPGWV VEYPTGKETG DFLALDLGGT NLRVVLVKLG
110 120 130 140 150
GNHDFDTTQN KYRLPDHLRT GTSEQLWSFI AKCLKEFVDE WYPDGVSEPL
160 170 180 190 200
PLGFTFSYPA SQKKINSGVL QRWTKGFDIE GVEGHDVVPM LQEQIEKLNI
210 220 230 240 250
PINVVALIND TTGTLVASLY TDPQTKMGII IGTGVNGAYY DVVSGIEKLE
260 270 280 290 300
GLLPEDIGPD SPMAINCEYG SFDNEHLVLP RTKYDVIIDE ESPRPGQQAF
310 320 330 340 350
EKMTSGYYLG EIMRLVLLDL YDSGFIFKDQ DISKLKEAYV MDTSYPSKIE
360 370 380 390 400
DDPFENLEDT DDLFKTNLNI ETTVVERKLI RKLAELVGTR AARLTVCGVS
410 420 430 440 450
AICDKRGYKT AHIAADGSVF NRYPGYKEKA AQALKDIYNW DVEKMEDHPI
460 470 480
QLVAAEDGSG VGAAIIACLT QKRLAAGKSV GIKGE
Length:485
Mass (Da):53,610
Last modified:January 23, 2007 - v3
Checksum:i3E93D80C92663FB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206A → R in CAA43855 (PubMed:8321195).Curated1

Mass spectrometryi

Molecular mass is 53476±5 Da from positions 2 - 485. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61680 Genomic DNA. Translation: CAA43855.1.
CR382124 Genomic DNA. Translation: CAH00663.1.
PIRiA48132.
RefSeqiXP_453567.1. XM_453567.1.

Genome annotation databases

EnsemblFungiiCAH00663; CAH00663; KLLA0_D11352g.
GeneIDi2893280.
KEGGikla:KLLA0D11352g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61680 Genomic DNA. Translation: CAA43855.1.
CR382124 Genomic DNA. Translation: CAH00663.1.
PIRiA48132.
RefSeqiXP_453567.1. XM_453567.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3O08X-ray2.00A/B1-485[»]
3O1BX-ray2.80A1-485[»]
3O1WX-ray1.66A/B1-485[»]
3O4WX-ray1.61A/B1-485[»]
3O5BX-ray1.97A/B1-485[»]
3O6WX-ray1.48A/B1-485[»]
3O80X-ray2.18A1-485[»]
3O8MX-ray1.42A1-485[»]
4JAXX-ray2.26A/B/C/D/E/F1-485[»]
ProteinModelPortaliP33284.
SMRiP33284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_453567.1.

PTM databases

iPTMnetiP33284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH00663; CAH00663; KLLA0_D11352g.
GeneIDi2893280.
KEGGikla:KLLA0D11352g.

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
HOGENOMiHOG000162670.
InParanoidiP33284.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Enzyme and pathway databases

UniPathwayiUPA00242.
BRENDAi2.7.1.1. 2825.
SABIO-RKP33284.

Miscellaneous databases

EvolutionaryTraceiP33284.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK_KLULA
AccessioniPrimary (citable) accession number: P33284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.